WO2001037781A2

WO2001037781A2 - A stable aqueous dispersion of nutrients

Info

Publication number: WO2001037781A2
Application number: PCT/US2000/041706
Authority: WO
Inventors: Scott Peters; Darryl H. Woods
Original assignee: Ingredient Innovations International
Priority date: 1999-10-28
Filing date: 2000-10-30
Publication date: 2001-05-31
Also published as: WO2001037781A8; WO2001037781A3; EP1227780A4; EP1227780A2

Abstract

This invention relates to a stable aqueous dispersion of nutrients and more particularly, to an aqueous dispersions of an active nutritional ingredient selected form (a) an isoflavone, (b) lycopene (c) lutein, (d) a Coenzyme Qn where n is an integer of 1 to 12, or (e) a mixture of any of the foregoing nutrients.

Description

A STABLE AQUEOUS DISPERSION OF NUTRIENTS

This application is a continuation-in-part of U.S. patent application Serial No. 09/685,366 filed October 10, 2000, which is incorporated herein by reference.

BACKGROUND OF THE INVENTION

FIELD OF THE INVENTION

This invention relates to a stable aqueous dispersion of nutrients, and more particularly, to a dispersion comprising an ingredient selected from (a) an isoflavone, (b) lycopene, (c) lutein, (d) a Coenzyme Q or (e) a mixture of the foregoing ingredients; and a stabilizer. DESCRIPTION OF THE RELATED ART

Nutritional ingredients, such as an isoflavone, e.g. a soybean derived isoflavone, are currently available in tablets or other dry forms because heretofore they could not be satisfactorily dispersed in water. The nutritional ingredients, such as an isoflavone, lycopene, lutein, Coezyme Q's, are not ordinarily dispersable in aqueous systems because they are only slightly water or oil soluble.

These nutritional ingredients are desirable for use in beverages and cosmetics, in the form of aqueous dispersions or liposomes. For example, isoflavone is employed to treat humans to lower cholesterol, to treat solid tumors and angiogenic diseases. Additionally, it reduces bone calcium loss and is an antioxidant which can reduce free-radical damage to cells. Accordingly, a means for rendering these ingredients water dispersible is needed and desired. SUMMARY OF THE INVENTION This invention relates to a stable suspension comprising a nutrient or nutritional ingredient and a stabilizer therefore dispersed in an aqueous system, e.g. a solvent comprising water. In particular, the ingredient is a nutrient selected from the group of (a) an isoflavone (b) lycopene, (c) lutein, (d) a Coenzyme Q_n, where n is an integer of 1 to 12, (e) a mixture of any of the foregoing ingredients.

DETAILED DESCRIPTION OF THE INVENTION This invention involves a stable aqueous suspension which comprises a nutrient and a nutrient stabilizer dispersed in an aqueous system or solvent, e.g. water. A suitable nutrient or nutritional ingredient is one which is suitable for therapeutic treatment of an animal, e.g. a human being, by ingestion, e.g. via a beverage, or a topical application, e.g. via a lotion or cream, but which is unfortunately typically insoluble or only slightly soluble in water at room temperature, e.g. 20°C to 25°C. It is these ingredients which are the subject of this invention. Some suitable nutrients or nutritional ingredients include (1) a compound of the formula,

where R is OH, β-glucoside, 6"-O-acetylglucoside, or 6"-O-malonylglucoside; R' is H

or OH; and R" is H or OCH₃; such as isoflavone, e.g. a soybean derived isoflavone, and a substituted isoflavone, such as daidzein, genistein and glycitein; (2) lycopene, (3) lutein, (4) a Coenzyme Q_n, where n is an integer of 1-12, e.g. Coenzyme Q₁₀, and (5) a mixture of any of the foregoing ingredients.

For purposes of the dispersions of this invention, which are intended for therapeutic use or as additives in association with therapeutic treatment of animals, e.g. a human, the particular nutrient or mixture of nutritional ingredients is present in the inventive aqueous dispersions in an effective nutritional amount, that is an amount which causes its desired nutritional or therapeutic effect.

The term "amount" as used herein refers to quantity or concentration as appropriate to the context. The amount of nutrient that constitutes a nutritional amount varies according to factors such as potency of the particular ingredient or mixture of ingredients, the route of administration and the mechanical system used to administer the dispersion. A nutritionally effective amount of a particular nutrient can be selected by those of ordinary skill in the art with due consideration of such factors. Generally, a nutritionally effective amount will be from .005 parts by weight to about 10 parts by weight based on 100 parts by weight of the dispersion.

A suitable aqueous system or medium is selected. A suitable aqueous system or medium for the dispersions of this invention include water and an aqueous solution of an organic alcohol of 1 to 6 carbon atoms, e.g. ethanol, propylene glycol, glycerin, etc., and a mixture of the foregoing; present in an amount of up to ten percent (10%) by weight. The aqueous system is one which will permit a stable dispersion to be formed therein when combined with the selected nutrient or mixture of nutrients, which in turn is destined to be combined with a suitable nutrient stabilizer. The aqueous system is present in an amount which affords the desired dispersion and is dependent upon the selected nutrient or mixture of nutritional ingredients with the selected nutrient stabilizer. Typically, the aqueous system comprises 75 to 95 weight percent of the dispersion.

A suitable stabilizer is selected. A suitable stabilizer includes (1) a lecithin, derived from soybean or egg which contain a complex mixture of phospholipids consisting mainly of phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, and phosphatidic acid combined with varying amounts of other substances such as triglycerides; the lecithin can be of standard grade or can be modified or refined lecithin e.g. deoiled, hydrogenated, hydroxylated, enzyme modified, acetylated, etc.; (2) a hydrocolloid, e.g. starch, xanthan gum, a pectin, gelatin, guar gum, carrageenan; (3) a surfactant, e.g. cetylpyridinlium chloride, polysorbate 80, sorbitan monostearate, polyglycerolesters, block copolymers of propylene oxide and ethylene oxide; (4) a Dowicil, a product of The Dow Chemical Company, e.g. Dowicil 200 of the formula

(5) a mixture of any of the foregoing stabilizers.

An aqueous dispersion of the selected nutrient comprises the nutrient stabilizer in an amount effective to stabilize the aqueous dispersion relative to an identical aqueous formulation or mixture not containing the nutrient stabilizer, such that the active ingredient does not settle, cream or flocculate after agitation so quickly as to prevent reproducibility, e.g. reproducible dosing. Reproducible application, e.g. dosing, can be achieved if the resultant aqueous suspension is substantially uniform for about 1 to 2 hours after agitation thereof.

The particular amount of stabilizer that constitutes an effective amount is dependent upon the particular stabilizer, the particular aqueous system or medium employed and the particular nutritional ingredient or mixture of ingredients employed. It is therefore not practical to enumerate a specific effective amount for use with specific dispersions or formulations of the invention, but such amount can readily be determined by those skilled in the art with due consideration of the factors set forth above. Generally, however, the stabilizer can be present in a formulation in an amount from about 0.05 percent by weight to about 10 percent by weight, more preferably about .2 percent to about 5 percent by weight, based on the weight of the dispersion or formulation.

Typically, the nutrient stabilizer, e.g. Dowicil 200 preservative, is combined with the aqueous system, e.g. water, at a temperature of 20° to 70°C and is mixed for 2 to 10 minutes. Thereafter the active ingredient or nutritional agent, e.g. isoflavone, is added thereto to form a mixture. The resultant mixture is then subjected to a high shear pressure treatment using any commercially available equipment for such purpose, e.g. a Microfluidizer, a product manufactured Microfluidics International

Corporation, 30 Ossipee Road, New ton, MA 02164, at a minimum shear pressure of

6500-7000 psi, preferably at a sheer pressure of 6500 to 8000 psi, and most preferably at a shear pressure of 7000 to 7500 psi, whereby a particle size to the active ingredient typically is less than 500nm, preferably less than 300 nm, most preferably less than 250 nm, to form the desired aqueous dispersion.

It is noted that the procedure described above can be modified, namely the stabilizer is added to the resultant aqueous dispersion, i.e. subsequent to the described a high shear pressure treatment of the mixture of the nutrient combined with the aqueous system.

The resultant aqueous nutrient dispersion can then be further formulated and administered to a patient, e.g. a mammal such as a human being, by any conventional means, such as topically, orally; etc. Typically the dispersion is combined with other drugs, adjuvants, etc. in the form of a cream or lotion, e.g. a cosmetic, or in the form of a liquid, e. g. a beverage.

EXAMPLES EXAMPLE 1 :

SoyLife 100, a product manufactured by Schouten, USA Inc., 3300 Edinborough Way, Minneapolis, MN 55435, contains ten percent by weight of soy derived isoflavone. Ten weight percent of this material was mixed or dispersed with 0.2% by weight of Dowicil 200 and 89.8% by weight of water using a laboratory mixer. The resultant mixture was fluidized twice through a Microfluidizer, Model Ml 10A, at 7,400-psi sheer pressure and 40 psi head pressure. The resultant slurry or dispersion was homogeneous and could easily be incorporated into a skin cream formulation.

EXAMPLE 2:

Prevastein HC, a product of Central Soya Company Inc., 1946 West Cook Road, Fort Wayne, _IN 46801, contains 40% by weight of isoflavone. Ten weight percent of this nutrient was combined with 0.1 percent by weight of potassium sorbate and 0.1%) by weight of citric acid in 89.8%> by weight of water. The resultant mixture was mixed with a laboratory mixer, then fluidized twice through the Microfluidizer as in Example 1, at 7500-psi sheer. Rhodigel (xanthan gum), 0.5% by weight, was added to the fluidized sample during high-speed mixing with a laboratory mixer. The resultant product was a thick viscous liquid that did not separate.

The resultant dispersion obtained in Example 2 was compared to samples prepared similarly to Example 2 but did not include a stabilizer and/or was not fluidized. When diluting the dispersion prepared in Example 2 with water (0.25-0.5% dispersion), the nutrient remained dispersed for over a week, with very little precipitation forming. The other samples prepared were diluted at similar levels as the Example 2 sample, but these dispersions precipitated much more quickly (within 1-2 hours).

Claims

WE CLAIM:

1. A stable suspension comprising, a nutrient selected from the group consisting of (a) an isoflavone, (b) lycopene, (c) lutein, (d) Coenzyme Q_n, were n is integer of 1 to 12; and (e) a mixture of any of the foregoing nutrients; a nutrient stabilizer; and an aqueous solvent system.

2. The suspension as defined in claim 1 where in said stabilizer is selected for the group consisting of (a') a lecithin, (b') a hydrocolloid, (c') a surfactant, (d') a Dowicil and (e') a mixture of any of the foregoing stabilizers.

3. The suspension as defined in claim 2 wherein said lecithin is selected from the group consisting of a lecithin derived from soybean or egg.

4. The suspension as defined in claim 2 wherein said hydrocolloid is selected from the group consisting of xanthan gum, starch, a pectin, gelatin, guar gum, carrageenan, methylcelluloses, hydroxypropyl celluloses, gum arabic and a mixture of the foregoing hydrocolloids.

5. The suspension as defined in claim 2 wherein said surfactant is selected from the group consisting of cetylpyridinium chloride, polysorbate 80, sorbitan monostearate, a polyglycerol ester, a block copolymer of propylene oxide and ethylene oxide and a mixture of any of the foregoing surfactants.

6. The suspension as defined in claim 3 wherein said stabilizer comprises said Dowicil.

7. A method of making a stabilized aqueous suspension of a nutrient which comprises:

(a) combining the nutrient with an aqueous medium to form an aqueous mixture; (b) combining a nutrient stabilizer with the nutrient; and

(c) adding a high shear pressure treatment to said aqueous mixture before or after step (b), above, to form the stabilized aqueous suspension.

8. The method as defined in claim 7 wherein the nutrient is selected form the group consisting of (a) an isoflavone, (b) lycopene, (c) lutein, (d) Coenzyme Q_n, were n is integer of 1 to 12; and (e) a mixture of any of the foregoing nutrients.

9. The method as defined in claim 7 wherein said nutrient stabilizer is selected from the group consisting of (a') a lecithin, (b') a hydrocolloid, (c') a surfactant, (d') a Dowicil and (e') a mixture of any of the foregoing stabilizers.

10. The method as defined in claim 9 wherein said lecithin is selected from the group consisting of a lecithin derived from soybean or egg.

11. The method as defined in claim 9 wherein said hydrocolloid is selected from the group consisting of xanthan gum, starch, a pectin, gelatin, guar gum, carrageenan, a methylcellulose, an hydroxypropyl cellulose, gum arabic and a mixture of the foregoing hydrocollids.

12. The method as defined in claim 9 wherein said surfactant is selected from the group consisting of cetylpyridinium chloride, polysorbate 80, sorbitan monostearate, a polyglycerol ester, a block copolymer of propylene oxide and ethylene oxide and a mixture of any of the foregoing surfactants.

13. The method as defined in claim 9 wherein said stabilizer comprises said Dowicil.