WO2001038583A3 - Capillary array-based sample screening - Google Patents

Capillary array-based sample screening Download PDF

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Publication number
WO2001038583A3
WO2001038583A3 PCT/US2000/032208 US0032208W WO0138583A3 WO 2001038583 A3 WO2001038583 A3 WO 2001038583A3 US 0032208 W US0032208 W US 0032208W WO 0138583 A3 WO0138583 A3 WO 0138583A3
Authority
WO
WIPO (PCT)
Prior art keywords
capillary array
based sample
sample screening
bioactive molecules
screening
Prior art date
Application number
PCT/US2000/032208
Other languages
French (fr)
Other versions
WO2001038583A2 (en
Inventor
William Michael Lafferty
Jay M Short
Martin Keller
Original Assignee
Diversa Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/444,112 external-priority patent/US6972183B1/en
Priority claimed from US09/687,219 external-priority patent/US6794127B1/en
Application filed by Diversa Corp filed Critical Diversa Corp
Priority to JP2001539924A priority Critical patent/JP2003515150A/en
Priority to EP00980740A priority patent/EP1144679A3/en
Priority to CA002360750A priority patent/CA2360750A1/en
Priority to AU17961/01A priority patent/AU1796101A/en
Priority to IL14425100A priority patent/IL144251A0/en
Publication of WO2001038583A2 publication Critical patent/WO2001038583A2/en
Publication of WO2001038583A3 publication Critical patent/WO2001038583A3/en
Priority to US10/151,469 priority patent/US20030219752A1/en

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50857—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates using arrays or bundles of open capillaries for holding samples
    • C—CHEMISTRY; METALLURGY
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
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    • B01J2219/00367—Pipettes capillary
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
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    • B01J2219/00603—Making arrays on substantially continuous surfaces
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    • B01J2219/0061—The surface being organic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
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    • B01J2219/00612—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports the surface being inorganic
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
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    • B01J2219/00603—Making arrays on substantially continuous surfaces
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    • B01J2219/00614—Delimitation of the attachment areas
    • B01J2219/00617—Delimitation of the attachment areas by chemical means
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    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00677—Ex-situ synthesis followed by deposition on the substrate
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    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
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    • B01J2219/00718—Type of compounds synthesised
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    • B01J2219/0074—Biological products
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
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    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
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    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries

Abstract

Provided are methods of screening and identification of bioactivities and bioactive molecules of interest using a capillary array system. More specifically, disclosed are methods of using optical detection and capillary array-based techniques for screening libraries and recovering bioactive molecules having a desired activity or a nucleic acid sequence encoding such bioactive molecules.
PCT/US2000/032208 1995-12-07 2000-11-22 Capillary array-based sample screening WO2001038583A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
JP2001539924A JP2003515150A (en) 1999-11-22 2000-11-22 Sample screening based on capillary array
EP00980740A EP1144679A3 (en) 1999-11-22 2000-11-22 Capillary array-based sample screening
CA002360750A CA2360750A1 (en) 1999-11-22 2000-11-22 Capillary array-based sample screening
AU17961/01A AU1796101A (en) 1999-11-22 2000-11-22 Capillary array-based sample screening
IL14425100A IL144251A0 (en) 1999-11-22 2000-11-22 Capillary array-based sample screening
US10/151,469 US20030219752A1 (en) 1995-12-07 2002-05-17 Novel antigen binding molecules for therapeutic, diagnostic, prophylactic, enzymatic, industrial, and agricultural applications, and methods for generating and screening thereof

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US09/444,112 US6972183B1 (en) 1997-06-16 1999-11-22 Capillary array-based enzyme screening
US09/444,112 1999-11-22
US09/687,219 US6794127B1 (en) 1997-06-16 2000-10-12 Capillary array-based sample screening
US09/687,219 2000-10-12

Related Child Applications (2)

Application Number Title Priority Date Filing Date
PCT/US1997/012239 Continuation-In-Part WO1998001581A1 (en) 1996-07-09 1997-07-09 Method of dna shuffling with polynucleotides produced by blockingor interrupting a synthesis or amplification process
US10/151,469 Continuation-In-Part US20030219752A1 (en) 1995-12-07 2002-05-17 Novel antigen binding molecules for therapeutic, diagnostic, prophylactic, enzymatic, industrial, and agricultural applications, and methods for generating and screening thereof

Publications (2)

Publication Number Publication Date
WO2001038583A2 WO2001038583A2 (en) 2001-05-31
WO2001038583A3 true WO2001038583A3 (en) 2001-10-11

Family

ID=27033783

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/032208 WO2001038583A2 (en) 1995-12-07 2000-11-22 Capillary array-based sample screening

Country Status (6)

Country Link
EP (1) EP1144679A3 (en)
JP (1) JP2003515150A (en)
AU (1) AU1796101A (en)
CA (1) CA2360750A1 (en)
IL (1) IL144251A0 (en)
WO (1) WO2001038583A2 (en)

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US8906618B2 (en) 2000-02-18 2014-12-09 The Board Of Trustees Of The Leland Stanford Junior University Apparatus and methods for parallel processing of micro-volume liquid reactions
US9266108B2 (en) 2004-03-12 2016-02-23 Life Technologies Corporation Nanoliter array loading
US9428800B2 (en) 2002-12-20 2016-08-30 Life Technologies Corporation Thermal cycling apparatus and method

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US7019827B2 (en) * 1997-06-16 2006-03-28 Diversa Corporation GigaMatrix holding tray having through-hole wells
DE10040857A1 (en) * 2000-08-11 2002-02-28 Jens P Fuerste Nucleic acid library or protein or peptide library
US20100261159A1 (en) 2000-10-10 2010-10-14 Robert Hess Apparatus for assay, synthesis and storage, and methods of manufacture, use, and manipulation thereof
EP1330306A2 (en) 2000-10-10 2003-07-30 BioTrove, Inc. Apparatus for assay, synthesis and storage, and methods of manufacture, use, and manipulation thereof
CA2450577A1 (en) * 2001-06-21 2003-01-03 Diversa Corporation Methods for the manufacture of pure single enantiomer compounds and for selecting enantioselective enzymes
JP2010207225A (en) * 2002-02-13 2010-09-24 Dow Global Technologies Inc Overexpression of extremozyme gene in pseudomonas bacterium and allied bacterium
WO2003068948A1 (en) * 2002-02-13 2003-08-21 Dow Global Technologies Inc. Over-expression of extremozyme genes in pseudomonads and closely related bacteria
US20060105453A1 (en) 2004-09-09 2006-05-18 Brenan Colin J Coating process for microfluidic sample arrays
JP2006061023A (en) * 2004-08-24 2006-03-09 Suntory Ltd Screening method using microchamber array and screening apparatus
NZ594810A (en) 2005-03-15 2012-12-21 Verenium Corp Cellulases, nucleic acids encoding them and methods for making and using them
DK2450439T3 (en) 2006-02-10 2014-02-10 Verenium Corp Cellulose-degrading enzymes, nucleic acids encoding them, and methods of preparing and utilizing them
CA2674721C (en) 2007-01-30 2018-04-03 Verenium Corporation Enzymes for the treatment of lignocellulosics, nucleic acids encoding them and methods for making and using them
US9657290B2 (en) 2012-07-03 2017-05-23 The Board Of Trustees Of The Leland Stanford Junior University Scalable bio-element analysis
US10370653B2 (en) 2015-02-22 2019-08-06 The Board Of Trustees Of The Leland Stanford Junior University Micro-screening apparatus, process, and products
SG10201504170UA (en) * 2015-05-27 2016-12-29 Jn Medsys Pte Ltd Method Of Evaporating Liquid In Micro-Capillaries
WO2018089953A1 (en) 2016-11-14 2018-05-17 Orca Biosystems, Inc. Methods and apparatuses for sorting target particles

Citations (7)

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