WO2001049839A3 - Method for expressing gene and use thereof - Google Patents

Method for expressing gene and use thereof Download PDF

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Publication number
WO2001049839A3
WO2001049839A3 PCT/JP2001/000048 JP0100048W WO0149839A3 WO 2001049839 A3 WO2001049839 A3 WO 2001049839A3 JP 0100048 W JP0100048 W JP 0100048W WO 0149839 A3 WO0149839 A3 WO 0149839A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein
rna
gene expression
dna
expressing gene
Prior art date
Application number
PCT/JP2001/000048
Other languages
French (fr)
Other versions
WO2001049839B1 (en
WO2001049839A2 (en
Inventor
Katsunobu Sakai
Makoto Hatakeyama
Keiji Umeda
Yositaka Nadachi
Masahiko Sudo
Original Assignee
Umeda Jimusho Ltd
Katsunobu Sakai
Makoto Hatakeyama
Keiji Umeda
Yositaka Nadachi
Masahiko Sudo
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Umeda Jimusho Ltd, Katsunobu Sakai, Makoto Hatakeyama, Keiji Umeda, Yositaka Nadachi, Masahiko Sudo filed Critical Umeda Jimusho Ltd
Priority to AU24069/01A priority Critical patent/AU2406901A/en
Publication of WO2001049839A2 publication Critical patent/WO2001049839A2/en
Publication of WO2001049839A3 publication Critical patent/WO2001049839A3/en
Publication of WO2001049839B1 publication Critical patent/WO2001049839B1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

The present invention relates to a method of converting one of DNA, RNA and protein to any one of DNA, RNA and protein by the novel gene expression routes which have not been known, a method of producing DNA, RNA or protein by the gene expression routes, a reagent having an action of promoting each reaction of the gene expression rotues, and a medicament for expressing an objective protein in vivo in the body of human or animal.
PCT/JP2001/000048 2000-01-07 2001-01-09 Method for expressing gene and use thereof WO2001049839A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU24069/01A AU2406901A (en) 2000-01-07 2001-01-09 Method for expressing gene and use thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2000002034 2000-01-07
JP2000-2034 2000-01-07

Publications (3)

Publication Number Publication Date
WO2001049839A2 WO2001049839A2 (en) 2001-07-12
WO2001049839A3 true WO2001049839A3 (en) 2002-01-03
WO2001049839B1 WO2001049839B1 (en) 2002-02-07

Family

ID=18531187

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2001/000048 WO2001049839A2 (en) 2000-01-07 2001-01-09 Method for expressing gene and use thereof

Country Status (2)

Country Link
AU (1) AU2406901A (en)
WO (1) WO2001049839A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002145785A (en) * 2000-11-06 2002-05-22 Hokkaido Gene digestion medicine having vaccine-similar action and enzyme
JP2003088387A (en) * 2001-09-19 2003-03-25 Japan Science & Technology Corp Method for synthesizing polynucleotide from protein by reverse translation and oligonucleotide used for the same

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1986003780A1 (en) * 1984-12-21 1986-07-03 Techniclone Research Partners I Method for electrically immortalizing lymphoid cells
WO1990011092A1 (en) * 1989-03-21 1990-10-04 Vical, Inc. Expression of exogenous polynucleotide sequences in a vertebrate
US5437291A (en) * 1993-08-26 1995-08-01 Univ Johns Hopkins Method for treating gastrointestinal muscle disorders and other smooth muscle dysfunction
WO1998018489A1 (en) * 1996-10-30 1998-05-07 The Uab Research Foundation Enhancement of tumor cell chemosensitivity and radiosensitivity using single chain intracellular antibodies

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1986003780A1 (en) * 1984-12-21 1986-07-03 Techniclone Research Partners I Method for electrically immortalizing lymphoid cells
WO1990011092A1 (en) * 1989-03-21 1990-10-04 Vical, Inc. Expression of exogenous polynucleotide sequences in a vertebrate
US5437291A (en) * 1993-08-26 1995-08-01 Univ Johns Hopkins Method for treating gastrointestinal muscle disorders and other smooth muscle dysfunction
WO1998018489A1 (en) * 1996-10-30 1998-05-07 The Uab Research Foundation Enhancement of tumor cell chemosensitivity and radiosensitivity using single chain intracellular antibodies

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CRAIG D ET AL: "Plasmid cDNA-directed protein synthesis in a coupled eukaryotic in vitro transcription-translation system", NUCLEIC ACIDS RESEARCH,GB,OXFORD UNIVERSITY PRESS, SURREY, vol. 20, no. 19, 1992, pages 4987 - 4995, XP002088195, ISSN: 0305-1048 *
SONNEMANN JUERGEN ET AL: "Elongation in a Dictyostelium in vitro translation system is affected by calmodulin antagonists.", FEBS (FEDERATION OF EUROPEAN BIOCHEMICAL SOCIETIES) LETTERS, vol. 329, no. 1-2, 1993, pages 183 - 188, XP001010426, ISSN: 0014-5793 *

Also Published As

Publication number Publication date
WO2001049839B1 (en) 2002-02-07
AU2406901A (en) 2001-07-16
WO2001049839A2 (en) 2001-07-12

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