WO2001079535A3 - Enzyme-based regeneration of surface-attached nucleic acids - Google Patents

Enzyme-based regeneration of surface-attached nucleic acids Download PDF

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Publication number
WO2001079535A3
WO2001079535A3 PCT/CA2001/000500 CA0100500W WO0179535A3 WO 2001079535 A3 WO2001079535 A3 WO 2001079535A3 CA 0100500 W CA0100500 W CA 0100500W WO 0179535 A3 WO0179535 A3 WO 0179535A3
Authority
WO
WIPO (PCT)
Prior art keywords
target
microarray
enzyme
probe
nucleic acids
Prior art date
Application number
PCT/CA2001/000500
Other languages
French (fr)
Other versions
WO2001079535A2 (en
Inventor
Michael Thompson
L Michelle Furtado
Original Assignee
Sensorchem Internat Corp
Michael Thompson
L Michelle Furtado
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sensorchem Internat Corp, Michael Thompson, L Michelle Furtado filed Critical Sensorchem Internat Corp
Priority to US10/240,932 priority Critical patent/US7101669B2/en
Priority to AU2001248195A priority patent/AU2001248195A1/en
Publication of WO2001079535A2 publication Critical patent/WO2001079535A2/en
Publication of WO2001079535A3 publication Critical patent/WO2001079535A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Abstract

Enzyme-based regeneration of surface-attached nucleic acids is described herein. Microarrays involving hybridization of a probe to a target are important tools for genetic analysis. Conventionally, a microarray is used for a single analysis, after which it is discarded. The invention relates to a process for regeneration of a microarray through enzymatic digestion of a target from a surface-attached probe using a nuclease to digest a single strand of a nucleic acid duplex with directional specificity starting from the free end of the target strand. For example, a probe oligonucleotide bound to a gene chip at the 5'-end hybridizes to a target nucleic acid, leaving the 5' end of the target open to 5'-3' digestion. Lambda-exonuclease (μ-exonuclease) cleaves single nucleotides from the 5' end of a duplex, progressing in the 5'-3' direction. Once the target strand is digested, the enzyme is rinsed from the microarray. The microarray is thus regenerated and ready for a subsequent use.
PCT/CA2001/000500 2000-04-12 2001-04-12 Enzyme-based regeneration of surface-attached nucleic acids WO2001079535A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US10/240,932 US7101669B2 (en) 2000-04-12 2001-04-12 Enzyme-based regeneration of surface-attached nucleic acids
AU2001248195A AU2001248195A1 (en) 2000-04-12 2001-04-12 Enzyme-based regeneration of surface-attached nucleic acids

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CA2,302,827 2000-04-12
CA002302827A CA2302827A1 (en) 2000-04-12 2000-04-12 Oligonucleotide and dna de-hybridization on surfaces

Publications (2)

Publication Number Publication Date
WO2001079535A2 WO2001079535A2 (en) 2001-10-25
WO2001079535A3 true WO2001079535A3 (en) 2003-02-13

Family

ID=4165674

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA2001/000500 WO2001079535A2 (en) 2000-04-12 2001-04-12 Enzyme-based regeneration of surface-attached nucleic acids

Country Status (4)

Country Link
US (1) US7101669B2 (en)
AU (1) AU2001248195A1 (en)
CA (1) CA2302827A1 (en)
WO (1) WO2001079535A2 (en)

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Publication number Priority date Publication date Assignee Title
KR100436554B1 (en) * 2001-04-17 2004-06-18 삼성전자주식회사 A Method for Improving a Sensitivity of Sensing Device in the Detection of a Hybridized Nucleic Acid
US7297553B2 (en) * 2002-05-28 2007-11-20 Nanosphere, Inc. Method for attachment of silylated molecules to glass surfaces
AU2002322458A1 (en) * 2001-07-13 2003-01-29 Nanosphere, Inc. Method for immobilizing molecules onto surfaces
DE102007044708A1 (en) * 2007-09-18 2009-03-19 Eads Deutschland Gmbh Apparatus and method for regenerating biosensors
EP2189793A1 (en) * 2008-11-21 2010-05-26 Micronas GmbH Method for regenerating a biosensor
ES2812261T3 (en) * 2016-08-01 2021-03-16 Hoffmann La Roche Procedures for the removal of adapter dimers from nucleic acid sequencing preparations
RU2693595C1 (en) * 2018-03-15 2019-07-03 Федеральное государственное бюджетное образовательное учреждение высшего образования "Юго-Западный государственный университет" (ЮЗГУ) Method of phase referencing generated pulse train to external trigger pulse

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US5518900A (en) * 1993-01-15 1996-05-21 Molecular Tool, Inc. Method for generating single-stranded DNA molecules
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Also Published As

Publication number Publication date
US7101669B2 (en) 2006-09-05
AU2001248195A1 (en) 2001-10-30
US20040101838A1 (en) 2004-05-27
WO2001079535A2 (en) 2001-10-25
CA2302827A1 (en) 2001-10-12

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