WO2001081370A3 - Histone h2a-derived peptides useful in gene delivery - Google Patents

Histone h2a-derived peptides useful in gene delivery Download PDF

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Publication number
WO2001081370A3
WO2001081370A3 PCT/EP2001/004621 EP0104621W WO0181370A3 WO 2001081370 A3 WO2001081370 A3 WO 2001081370A3 EP 0104621 W EP0104621 W EP 0104621W WO 0181370 A3 WO0181370 A3 WO 0181370A3
Authority
WO
WIPO (PCT)
Prior art keywords
gene delivery
histone
derived peptides
nucleus
dna
Prior art date
Application number
PCT/EP2001/004621
Other languages
French (fr)
Other versions
WO2001081370A2 (en
Inventor
Danuta Balicki
Ernest Beutler
Original Assignee
Novartis Ag
Novartis Erfind Verwalt Gmbh
Scripps Research Inst
Danuta Balicki
Ernest Beutler
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novartis Ag, Novartis Erfind Verwalt Gmbh, Scripps Research Inst, Danuta Balicki, Ernest Beutler filed Critical Novartis Ag
Priority to US10/258,637 priority Critical patent/US20040102606A1/en
Priority to AU2001256319A priority patent/AU2001256319A1/en
Priority to EP01929598A priority patent/EP1280822A2/en
Publication of WO2001081370A2 publication Critical patent/WO2001081370A2/en
Publication of WO2001081370A3 publication Critical patent/WO2001081370A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity

Abstract

The present invention provides a novel gene delivery system in which a gene delivery facilitating peptide, generally derived from Histone H2A, is complexed with a nucleic acid for efficient and stable delivery of the nucleic acid into a cell, ultimately to the nucleus. Such peptide-mediated gene delivery is based on the principal that unneutralized positive charges on the histone are bound electrostatically both by the negatively charged phosphate backbone of DNA and that nuclear targeting signals in histones improve trafficking of the DNA to the nucleus for transcription.
PCT/EP2001/004621 2000-04-24 2001-04-24 Histone h2a-derived peptides useful in gene delivery WO2001081370A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US10/258,637 US20040102606A1 (en) 2001-04-24 2001-04-24 Histone H2A -derived peptides useful in gene delivery
AU2001256319A AU2001256319A1 (en) 2000-04-24 2001-04-24 Histone h2a-derived peptides useful in gene delivery
EP01929598A EP1280822A2 (en) 2000-04-24 2001-04-24 Histone h2a-derived peptides useful in gene delivery

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US19915300P 2000-04-24 2000-04-24
US60/199,153 2000-04-24

Publications (2)

Publication Number Publication Date
WO2001081370A2 WO2001081370A2 (en) 2001-11-01
WO2001081370A3 true WO2001081370A3 (en) 2002-06-27

Family

ID=22736440

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/004621 WO2001081370A2 (en) 2000-04-24 2001-04-24 Histone h2a-derived peptides useful in gene delivery

Country Status (3)

Country Link
EP (1) EP1280822A2 (en)
AU (1) AU2001256319A1 (en)
WO (1) WO2001081370A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060040882A1 (en) * 2004-05-04 2006-02-23 Lishan Chen Compostions and methods for enhancing delivery of nucleic acids into cells and for modifying expression of target genes in cells
WO2007030619A2 (en) * 2005-09-08 2007-03-15 Nastech Pharmaceutical Company Inc. Pharmaceutical compositions for delivery of ribonucleic acid to a cell
WO2019219721A1 (en) 2018-05-18 2019-11-21 F. Hoffmann-La Roche Ag Targeted intracellular delivery of large nucleic acids

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996014424A1 (en) * 1994-11-08 1996-05-17 Medical Research Council Dna transfer method
US5744335A (en) * 1995-09-19 1998-04-28 Mirus Corporation Process of transfecting a cell with a polynucleotide mixed with an amphipathic compound and a DNA-binding protein
WO1998040502A1 (en) * 1997-03-14 1998-09-17 Life Technologies, Inc. Peptide-enhanced transfections

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100314721B1 (en) * 1998-01-22 2001-11-23 김일웅 Biologically active peptides

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996014424A1 (en) * 1994-11-08 1996-05-17 Medical Research Council Dna transfer method
US5744335A (en) * 1995-09-19 1998-04-28 Mirus Corporation Process of transfecting a cell with a polynucleotide mixed with an amphipathic compound and a DNA-binding protein
WO1998040502A1 (en) * 1997-03-14 1998-09-17 Life Technologies, Inc. Peptide-enhanced transfections

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ANAL CHIM ACTA (1991) 249(1) 215-25 *
DATABASE CA [online] CHEMICAL ABSTRACTS SERVICE, COLUMBUS, OHIO, US; MILLER, CHARLEEN ET AL.: "SYNTHESIS PURIFICATION AND CHARACTERIZATION OF RAT HISTONE H2A (1-53)-NH2", XP002195949, retrieved from STN Database accession no. 115:227660 *
KIM H S ET AL: "CDNA CLONING AND CHARACTERIZATION OF BUFORIN I, AN ANTIMICROBIAL PEPTIDE: A CLEAVAGE PRODUCT OF HISTONE H2A", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US, vol. 229, 1996, pages 381 - 387, XP000941591, ISSN: 0006-291X *
PARK CHAN BAE ET AL: "Mechanism of action of the antimicrobial peptide buforin II: Buforin II kills microorganisms by penetrating the cell membrane and inhibiting cellular functions.", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 244, no. 1, 6 March 1998 (1998-03-06), pages 253 - 257, XP002195948, ISSN: 0006-291X *
See also references of EP1280822A2 *

Also Published As

Publication number Publication date
EP1280822A2 (en) 2003-02-05
WO2001081370A2 (en) 2001-11-01
AU2001256319A1 (en) 2001-11-07

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