WO2001081566A3 - Apparatus and method for separating and purifying polynucleotides - Google Patents

Apparatus and method for separating and purifying polynucleotides Download PDF

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Publication number
WO2001081566A3
WO2001081566A3 PCT/US2001/012913 US0112913W WO0181566A3 WO 2001081566 A3 WO2001081566 A3 WO 2001081566A3 US 0112913 W US0112913 W US 0112913W WO 0181566 A3 WO0181566 A3 WO 0181566A3
Authority
WO
WIPO (PCT)
Prior art keywords
separation medium
target polynucleotide
separation
polynucleotide
medium
Prior art date
Application number
PCT/US2001/012913
Other languages
French (fr)
Other versions
WO2001081566A2 (en
Inventor
Douglas T Gjerde
Christopher P Hanna
David Hornby
Mark Dickman
Benjamin J Legendre Jr
Paul Taylor
Robert Haefele
Arezou Azarani
Original Assignee
Transgenomic Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/557,424 external-priority patent/US6475388B1/en
Priority claimed from US09/764,041 external-priority patent/US6642374B2/en
Application filed by Transgenomic Inc filed Critical Transgenomic Inc
Priority to EP01932598A priority Critical patent/EP1274837A2/en
Priority to AU2001259113A priority patent/AU2001259113A1/en
Publication of WO2001081566A2 publication Critical patent/WO2001081566A2/en
Publication of WO2001081566A3 publication Critical patent/WO2001081566A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)

Abstract

The instant invention provides a non-HPLC chromatographic method for purifying a target polynucleotide comprising the steps of: applying the target polynucleotide to a separation medium having a non-polar separation surface in the presence of a counterion agent, whereby the polynucleotide is bound to the separation medium; eluting the target polynucleotide from the separation medium by passing through the separation medium an elution solution containing a concentration of organic solvent sufficient to elute the target polynucleotide from the separation medium; and collecting the eluted target polynucleotide. The separation medium can be supported in any of a variety of containers, non-limiting preferred examples of which include spin columns and vacuum trays. The invention is particularly useful for the separation of RNA and single and double stranded DNA. In preferred embodiments of the invention the purification is accomplished under conditions that are substantially free of multivalent cations capable of interfering with polynucleotide separations.
PCT/US2001/012913 2000-04-21 2001-04-20 Apparatus and method for separating and purifying polynucleotides WO2001081566A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP01932598A EP1274837A2 (en) 2000-04-21 2001-04-20 Apparatus and method for separating and purifying polynucleotides
AU2001259113A AU2001259113A1 (en) 2000-04-21 2001-04-20 Apparatus and method for separating and purifying polynucleotides

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US09/557,424 2000-04-21
US09/557,424 US6475388B1 (en) 1996-11-13 2000-04-21 Method and system for RNA analysis by matched ion polynucleotide chromatography
US09/764,041 2001-01-16
US09/764,041 US6642374B2 (en) 1997-04-25 2001-01-16 Process for separation of polynucleotide fragments

Publications (2)

Publication Number Publication Date
WO2001081566A2 WO2001081566A2 (en) 2001-11-01
WO2001081566A3 true WO2001081566A3 (en) 2002-05-10

Family

ID=27071415

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/012913 WO2001081566A2 (en) 2000-04-21 2001-04-20 Apparatus and method for separating and purifying polynucleotides

Country Status (3)

Country Link
EP (1) EP1274837A2 (en)
AU (1) AU2001259113A1 (en)
WO (1) WO2001081566A2 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4447365A (en) * 1981-11-19 1984-05-08 International Flavors & Fragrances Inc. 2-Ethyl hexyl and isobornyl methyl carbonates
US6521411B2 (en) 2000-09-28 2003-02-18 Transgenomic, Inc. Method and system for the preparation of cDNA
US6812341B1 (en) 2001-05-11 2004-11-02 Ambion, Inc. High efficiency mRNA isolation methods and compositions
GB0619442D0 (en) * 2006-10-02 2006-11-08 Q Chip Ltd Beads for use in reactions for the amplification and/or synthesis of a polynucleotide
GB0810990D0 (en) * 2008-06-16 2008-07-23 Q Chip Ltd Device and method of making solid beads
EP2983804A4 (en) * 2013-03-15 2017-03-01 Moderna Therapeutics, Inc. Ion exchange purification of mrna
US11377470B2 (en) 2013-03-15 2022-07-05 Modernatx, Inc. Ribonucleic acid purification
US10077439B2 (en) 2013-03-15 2018-09-18 Modernatx, Inc. Removal of DNA fragments in mRNA production process
US10138507B2 (en) 2013-03-15 2018-11-27 Modernatx, Inc. Manufacturing methods for production of RNA transcripts
WO2016057950A1 (en) 2014-10-09 2016-04-14 Illumina, Inc. Method and device for separating immiscible liquids to effectively isolate at least one of the liquids

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998056798A1 (en) * 1997-06-10 1998-12-17 Transgenomic, Inc. Improved process for performing polynucleotide separations
WO1999019514A1 (en) * 1997-10-09 1999-04-22 Transgenomic, Inc. Modifying double stranded dna to enhance separations by matched ion polynucleotide chromatography
US5986085A (en) * 1997-04-25 1999-11-16 Transgenomic, Inc. Matched ion polynucleotide chromatography (MIPC) process for separation of polynucleotide fragments
US6265168B1 (en) * 1998-10-06 2001-07-24 Transgenomic, Inc. Apparatus and method for separating and purifying polynucleotides

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5986085A (en) * 1997-04-25 1999-11-16 Transgenomic, Inc. Matched ion polynucleotide chromatography (MIPC) process for separation of polynucleotide fragments
WO1998056798A1 (en) * 1997-06-10 1998-12-17 Transgenomic, Inc. Improved process for performing polynucleotide separations
WO1999019514A1 (en) * 1997-10-09 1999-04-22 Transgenomic, Inc. Modifying double stranded dna to enhance separations by matched ion polynucleotide chromatography
US6265168B1 (en) * 1998-10-06 2001-07-24 Transgenomic, Inc. Apparatus and method for separating and purifying polynucleotides

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
YANG ET AL.: "Technical note: Improved DNA extraction from ancient bones using silica-based spin columns", AMERICAN JOURNAL OF PHYSICAL ANTHROPOLOGY, vol. 105, no. 4, April 1998 (1998-04-01), pages 539 - 543, XP008000703 *

Also Published As

Publication number Publication date
EP1274837A2 (en) 2003-01-15
WO2001081566A2 (en) 2001-11-01
AU2001259113A1 (en) 2001-11-07

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