WO2001090315A3 - Production of neurons from stem cells - Google Patents

Production of neurons from stem cells Download PDF

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Publication number
WO2001090315A3
WO2001090315A3 PCT/CA2001/000756 CA0100756W WO0190315A3 WO 2001090315 A3 WO2001090315 A3 WO 2001090315A3 CA 0100756 W CA0100756 W CA 0100756W WO 0190315 A3 WO0190315 A3 WO 0190315A3
Authority
WO
WIPO (PCT)
Prior art keywords
stem cells
medium
neurons
production
heat
Prior art date
Application number
PCT/CA2001/000756
Other languages
French (fr)
Other versions
WO2001090315A2 (en
Inventor
Harold A Robertson
Cindee Leopold
Victor Rafuse
Original Assignee
Novaneuron Inc
Harold A Robertson
Cindee Leopold
Victor Rafuse
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novaneuron Inc, Harold A Robertson, Cindee Leopold, Victor Rafuse filed Critical Novaneuron Inc
Priority to AU2001261984A priority Critical patent/AU2001261984A1/en
Publication of WO2001090315A2 publication Critical patent/WO2001090315A2/en
Publication of WO2001090315A3 publication Critical patent/WO2001090315A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0619—Neurons
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/08—Coculture with; Conditioned medium produced by cells of the nervous system

Abstract

A method is provided for producing neurons by incubating stem cells in a growth medium containing a growth factor; separating said growth medium from said stem cell, heat treating said growth medium to produce a heat-treated medium and subsequently incubating said stem cells in a treated medium which includes said heat-treated medium. Also provided are neurons produced by the present method and conditioned medium produced by the present method, and uses thereof.
PCT/CA2001/000756 2000-05-25 2001-05-25 Production of neurons from stem cells WO2001090315A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001261984A AU2001261984A1 (en) 2000-05-25 2001-05-25 Production of neurons from stem cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US20680700P 2000-05-25 2000-05-25
US60/206,807 2000-05-25

Publications (2)

Publication Number Publication Date
WO2001090315A2 WO2001090315A2 (en) 2001-11-29
WO2001090315A3 true WO2001090315A3 (en) 2002-08-01

Family

ID=22768053

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA2001/000756 WO2001090315A2 (en) 2000-05-25 2001-05-25 Production of neurons from stem cells

Country Status (2)

Country Link
AU (1) AU2001261984A1 (en)
WO (1) WO2001090315A2 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62107796A (en) * 1985-11-07 1987-05-19 Morinaga Milk Ind Co Ltd Production of novel human granulocyte-monocyte stem cell proliferation factor
JPH07265068A (en) * 1994-03-31 1995-10-17 Snow Brand Milk Prod Co Ltd Additive for culture medium used in cell culture
US5851832A (en) * 1991-07-08 1998-12-22 Neurospheres, Ltd. In vitro growth and proliferation of multipotent neural stem cells and their progeny

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62107796A (en) * 1985-11-07 1987-05-19 Morinaga Milk Ind Co Ltd Production of novel human granulocyte-monocyte stem cell proliferation factor
US5851832A (en) * 1991-07-08 1998-12-22 Neurospheres, Ltd. In vitro growth and proliferation of multipotent neural stem cells and their progeny
JPH07265068A (en) * 1994-03-31 1995-10-17 Snow Brand Milk Prod Co Ltd Additive for culture medium used in cell culture

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
DAADI MARCEL M ET AL: "Generation of tyrosine hydroxylase-producing neurons from precursors of the embryonic and adult forebrain.", JOURNAL OF NEUROSCIENCE, vol. 19, no. 11, 1 June 1999 (1999-06-01), pages 4484 - 4497, XP002191901, ISSN: 0270-6474 *
DATABASE BIOSIS BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; XP002191904 *
DATABASE WPI Section Ch Week 198725, Derwent World Patents Index; Class B04, AN 1987-175153, XP002191905 *
DATABASE WPI Section Ch Week 199550, Derwent World Patents Index; Class B04, AN 1995-388680, XP002191906 *
FERNANDES K: "Recent advances in neural stem cell technologies review", NEURAL NOTES, vol. 19, 10 November 2001 (2001-11-10), XP002191903 *
KAWASAKI HIROSHI ET AL: "Induction of midbrain dopaminergic neurons from ES cells by stromal cell-derived inducing activity.", NEURON, vol. 28, no. 1, October 2000 (2000-10-01), pages 31 - 40, XP002191902, ISSN: 0896-6273 *
REYNOLDS B A ET AL: "CLONAL AND POPULATION ANALYSES DEMONSTRATE THAT AN EGF-RESPONSIVE MAMMALIAN EMBRYONIC CNS PRECURSOR IS A STEM CELL", DEVELOPMENTAL BIOLOGY, ACADEMIC PRESS, NEW YORK, NY, US, vol. 175, 1996, pages 1 - 13, XP000916284, ISSN: 0012-1606 *
SHINGO T ET AL: "Robust induction of tyrosine hydroxylase in embryonic and adult striatal neural stem cell-derived neurons in defined media and the absence of gene transfer.", SOCIETY FOR NEUROSCIENCE ABSTRACTS, vol. 26, no. 1-2, 2000, 30th Annual Meeting of the Society of Neuroscience;New Orleans, LA, USA; November 04-09, 2000, pages abstract - 312.18, XP002203033 *
WEISS S ET AL: "Is there a neural stem cell in the mammalian forebrain?", TRENDS IN NEUROSCIENCE, ELSEVIER, AMSTERDAM, NL, vol. 19, no. 9, 1996, pages 387 - 393, XP002098734, ISSN: 0166-2236 *

Also Published As

Publication number Publication date
WO2001090315A2 (en) 2001-11-29
AU2001261984A1 (en) 2001-12-03

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