WO2001094635A3 - Integrated active flux microfluidic devices and methods - Google Patents

Integrated active flux microfluidic devices and methods Download PDF

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Publication number
WO2001094635A3
WO2001094635A3 PCT/US2001/018400 US0118400W WO0194635A3 WO 2001094635 A3 WO2001094635 A3 WO 2001094635A3 US 0118400 W US0118400 W US 0118400W WO 0194635 A3 WO0194635 A3 WO 0194635A3
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WIPO (PCT)
Prior art keywords
molecules
proteins
dna
polynucleotides
methods
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Application number
PCT/US2001/018400
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French (fr)
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WO2001094635A9 (en
WO2001094635A2 (en
Inventor
Stephen R Quake
Hou-Pu Chou
Original Assignee
California Inst Of Techn
Stephen R Quake
Hou-Pu Chou
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Publication date
Application filed by California Inst Of Techn, Stephen R Quake, Hou-Pu Chou filed Critical California Inst Of Techn
Priority to EP01942042A priority Critical patent/EP1290226A2/en
Priority to AU2001275340A priority patent/AU2001275340A1/en
Priority to JP2002502175A priority patent/JP2003536058A/en
Publication of WO2001094635A2 publication Critical patent/WO2001094635A2/en
Publication of WO2001094635A9 publication Critical patent/WO2001094635A9/en
Publication of WO2001094635A3 publication Critical patent/WO2001094635A3/en

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    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/11—Automated chemical analysis
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/145555—Hetero-N
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/17—Nitrogen containing
    • Y10T436/173845—Amine and quaternary ammonium
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/2575—Volumetric liquid transfer

Abstract

The invention relates to a microfabricated device for the rapid detection of DNA, proteins or other molecules associated with a particular disease. The devices and methods of the invention can be used for the simultaneous diagnosis of multiple diseases by detecting molecules (e.g. amounts of molecules), such as polynucleotides (e.g., DNA) or proteins (e.g., antibodies), by measuring the signal of a detectable reporter associated with hybridized polynucleotides or antigen/antibody complex. In the microfabricated device according to the invention, detection of the presence of molecules (i.e., polynucleotides, proteins, or antigen/antibody complexes) are correlated to a hybridization signal from an optically-detectable (e.g. fluorescent) reporter associated with the bound molecules. These hybridization signals can be detected by any suitable means, for example optical, and can be stored for example in a computer as a representation of the presence of a particular gene. Hybridization probes can be immobilized on a substrate that forms part of or is exposed to a channel or channels of the device that form a closed loop, for circulation of sample to actively contact complementary probes. Universal chips according to the invention can be fabricated not only with DNA but also with other molecules such as RNA, proteins, peptide nucleic acid (PNA) and polyamide molecules.
PCT/US2001/018400 2000-06-05 2001-06-05 Integrated active flux microfluidic devices and methods WO2001094635A2 (en)

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AU2001275340A AU2001275340A1 (en) 2000-06-05 2001-06-05 Integrated active flux microfluidic devices and methods
JP2002502175A JP2003536058A (en) 2000-06-05 2001-06-05 Integrated active flux microfluidic device and method

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US20924300P 2000-06-05 2000-06-05
US60/209,243 2000-06-05
US21130900P 2000-06-13 2000-06-13
US60/211,309 2000-06-13
US24936000P 2000-11-16 2000-11-16
US60/249,360 2000-11-16
US09/724,548 2000-11-28
US09/724,548 US7351376B1 (en) 2000-06-05 2000-11-28 Integrated active flux microfluidic devices and methods

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