WO2001096862A3 - Method for increasing luminescence assay sensitivity - Google Patents

Method for increasing luminescence assay sensitivity Download PDF

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Publication number
WO2001096862A3
WO2001096862A3 PCT/US2001/018363 US0118363W WO0196862A3 WO 2001096862 A3 WO2001096862 A3 WO 2001096862A3 US 0118363 W US0118363 W US 0118363W WO 0196862 A3 WO0196862 A3 WO 0196862A3
Authority
WO
WIPO (PCT)
Prior art keywords
assay sensitivity
luminescence assay
increasing luminescence
increasing
analyte
Prior art date
Application number
PCT/US2001/018363
Other languages
French (fr)
Other versions
WO2001096862A2 (en
Inventor
Erika Hawkins
John M Centanni
Jacqueline Sankbeil
Keith V Wood
Original Assignee
Promega Corp
Erika Hawkins
John M Centanni
Jacqueline Sankbeil
Keith V Wood
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=24364121&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO2001096862(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Promega Corp, Erika Hawkins, John M Centanni, Jacqueline Sankbeil, Keith V Wood filed Critical Promega Corp
Priority to AU7532501A priority Critical patent/AU7532501A/en
Priority to CA002411179A priority patent/CA2411179A1/en
Priority to AU2001275325A priority patent/AU2001275325B2/en
Priority to AT01942027T priority patent/ATE496298T1/en
Priority to EP01942027A priority patent/EP1297337B1/en
Priority to JP2002510941A priority patent/JP4685325B2/en
Priority to DE60143888T priority patent/DE60143888D1/en
Publication of WO2001096862A2 publication Critical patent/WO2001096862A2/en
Publication of WO2001096862A3 publication Critical patent/WO2001096862A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/902—Oxidoreductases (1.)
    • G01N2333/90241—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/968—High energy substrates, e.g. fluorescent, chemiluminescent, radioactive
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/975—Kit

Abstract

A method for increasing the sensitivity of a luminescent assay comprising carrying out the assay in the presence of an organic compound that reduces luminescence that is not dependent on the presence of an analyte by at least about 10 fold, and that reduces luminescence that is dependent on the presence of an analyte by less than about 7 fold.
PCT/US2001/018363 2000-06-09 2001-06-07 Method for increasing luminescence assay sensitivity WO2001096862A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
AU7532501A AU7532501A (en) 2000-06-09 2001-06-07 Method for increasing luminescence assay sensitivity
CA002411179A CA2411179A1 (en) 2000-06-09 2001-06-07 Method for increasing luminescence assay sensitivity
AU2001275325A AU2001275325B2 (en) 2000-06-09 2001-06-07 Method for increasing luminescence assay sensitivity
AT01942027T ATE496298T1 (en) 2000-06-09 2001-06-07 METHOD TO INCREASE THE SENSITIVITY OF A LUMINESSENCE ASSAY
EP01942027A EP1297337B1 (en) 2000-06-09 2001-06-07 Method for increasing luminescence assay sensitivity
JP2002510941A JP4685325B2 (en) 2000-06-09 2001-06-07 Methods to increase luminescence assay sensitivity
DE60143888T DE60143888D1 (en) 2000-06-09 2001-06-07 METHOD TO INCREASE THE SENSITIVITY OF LUMINESIS ASSAY

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/590,884 2000-06-09
US09/590,884 US7118878B1 (en) 2000-06-09 2000-06-09 Method for increasing luminescence assay sensitivity

Publications (2)

Publication Number Publication Date
WO2001096862A2 WO2001096862A2 (en) 2001-12-20
WO2001096862A3 true WO2001096862A3 (en) 2002-07-18

Family

ID=24364121

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/018363 WO2001096862A2 (en) 2000-06-09 2001-06-07 Method for increasing luminescence assay sensitivity

Country Status (8)

Country Link
US (3) US7118878B1 (en)
EP (1) EP1297337B1 (en)
JP (2) JP4685325B2 (en)
AT (1) ATE496298T1 (en)
AU (2) AU2001275325B2 (en)
CA (1) CA2411179A1 (en)
DE (1) DE60143888D1 (en)
WO (1) WO2001096862A2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9139836B2 (en) 2010-11-02 2015-09-22 Promega Corporation Imidazo[1,2-a]pyrazine derivatives
US9487520B2 (en) 2010-11-02 2016-11-08 Promega Corporation Coelenterazine derivatives and methods of using same

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7118878B1 (en) 2000-06-09 2006-10-10 Promega Corporation Method for increasing luminescence assay sensitivity
AU2003267245C1 (en) 2002-09-20 2008-05-29 Promega Corporation Luminescence-based methods and probes for measuring cytochrome P450 activity
EP2325328B1 (en) 2002-12-23 2014-02-12 Promega Corporation Improved luciferase-based assays
US20040224377A1 (en) * 2003-02-12 2004-11-11 Erika Hawkins Compositions and methods to quench light from optical reactions
EP3312292B1 (en) 2005-05-31 2019-09-25 Promega Corporation Luminogenic and fluorogenic compounds and methods to detect molecules or conditions
US20100129840A1 (en) * 2006-10-13 2010-05-27 Charm Scineces, Inc Method and Apparatus for Reducing Luminescent Test Result Interferences
WO2008118445A1 (en) * 2007-03-26 2008-10-02 Promega Corporation Methods to quench light from optical reactions
US8431698B2 (en) * 2007-08-29 2013-04-30 Biolume Inc. Bioluminescent endoscopy methods and compounds
ES2390395T3 (en) * 2007-10-29 2012-11-12 Perkinelmer Health Sciences B.V. Methods, reagents and kits for the luciferase assay
JP5597200B2 (en) * 2008-08-18 2014-10-01 プロメガ コーポレイション Compounds producing luminescence and methods for detecting cytochrome P4503A enzyme
ES2559505T3 (en) 2009-05-01 2016-02-12 Promega Corporation Synthetic Oplophorus luciferases with greater light emission
US9856510B2 (en) * 2010-12-14 2018-01-02 Stichting Katholieke Universiteit Chemiluminescence-based haemostasis assay
US20120204906A1 (en) * 2011-02-15 2012-08-16 3M Innovative Properties Company Method of controlling a decontamination process
CN104685346B (en) 2012-05-02 2018-05-29 查尔斯河实验室公司 Cell capture system and its use
JP6285915B2 (en) 2012-05-02 2018-02-28 チャールズ リバー ラボラトリーズ， インコーポレイテッド Life-and-death discrimination staining method
US9709500B2 (en) 2012-05-02 2017-07-18 Charles River Laboratories, Inc. Optical method for detecting viable microorganisms in a cell sample
JP6588917B2 (en) 2014-01-29 2019-10-09 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ Pro-matrix for live cell applications
JP6703484B2 (en) 2014-01-29 2020-06-03 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ Quinone-masked probe as a labeling reagent for cellular uptake measurements
EP3428277B1 (en) 2016-03-11 2023-05-10 National Institute of Advanced Industrial Science and Technology Light-emitting enzyme protein
JP7350713B2 (en) 2017-08-04 2023-09-26 プロメガ コーポレイション Compositions and methods for stabilizing benzothiazole luciferin analogs
CN113891895A (en) * 2019-04-10 2022-01-04 普洛麦格公司 Compositions and methods for detecting analytes using bioluminescence

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Publication number Priority date Publication date Assignee Title
EP0437013A2 (en) * 1989-04-10 1991-07-17 Ela Technologies Inc. Luminescent assays
US5629168A (en) * 1992-02-10 1997-05-13 British Technology Group Limited Chemiluminescent enhancers
US5744307A (en) * 1993-02-26 1998-04-28 Mochida Pharmaceutical Co., Ltd. Method for measuring adenyl group-containing susbstances

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DE2828658C3 (en) 1978-06-29 1981-10-22 Lkb-Produkter Ab, Stockholm Method for the photometric determination of subunit B of creatine kinase and reagent therefor
DE2901919A1 (en) 1979-01-18 1980-07-24 Berthold Lab Prof Dr APPLICATION OF A - IN PARTICULAR AUTOMATIC - METHOD FOR LIGHT MEASUREMENT AND DEVELOPMENTS OF THIS METHOD AND DEVICES FOR IMPLEMENTING IT
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US5744320A (en) * 1995-06-07 1998-04-28 Promega Corporation Quenching reagents and assays for enzyme-mediated luminescence
US6602657B1 (en) 1995-12-28 2003-08-05 Tropix, Inc. Multiple reporter gene assay
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US5814504A (en) * 1996-08-22 1998-09-29 Kikkoman Corporation Protein involved in regenerating firefly luciferin
JPH1087621A (en) 1996-09-13 1998-04-07 Sankyo Co Ltd Enhancer for lucigenin chemiluminescence
US6200762B1 (en) 1997-08-01 2001-03-13 Aurora Biosciences Corporation Photon reducing agents and compositions for fluorescence assays
US6221612B1 (en) 1997-08-01 2001-04-24 Aurora Biosciences Corporation Photon reducing agents for use in fluorescence assays
ATE261583T1 (en) 1999-09-01 2004-03-15 Invitrogen Corp PHOTON DAMPER FOR FLUORESCENCE ASSAY
US7118878B1 (en) 2000-06-09 2006-10-10 Promega Corporation Method for increasing luminescence assay sensitivity
US6867005B2 (en) * 2001-10-24 2005-03-15 Beckman Coulter, Inc. Method and apparatus for increasing the dynamic range and accuracy of binding assays
US20040224377A1 (en) 2003-02-12 2004-11-11 Erika Hawkins Compositions and methods to quench light from optical reactions

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0437013A2 (en) * 1989-04-10 1991-07-17 Ela Technologies Inc. Luminescent assays
US5629168A (en) * 1992-02-10 1997-05-13 British Technology Group Limited Chemiluminescent enhancers
US5744307A (en) * 1993-02-26 1998-04-28 Mochida Pharmaceutical Co., Ltd. Method for measuring adenyl group-containing susbstances

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9139836B2 (en) 2010-11-02 2015-09-22 Promega Corporation Imidazo[1,2-a]pyrazine derivatives
US9487520B2 (en) 2010-11-02 2016-11-08 Promega Corporation Coelenterazine derivatives and methods of using same

Also Published As

Publication number Publication date
US7078181B2 (en) 2006-07-18
US7108996B2 (en) 2006-09-19
WO2001096862A2 (en) 2001-12-20
DE60143888D1 (en) 2011-03-03
ATE496298T1 (en) 2011-02-15
EP1297337A2 (en) 2003-04-02
AU2001275325B2 (en) 2006-10-05
CA2411179A1 (en) 2001-12-20
JP4685325B2 (en) 2011-05-18
JP2011064690A (en) 2011-03-31
US7118878B1 (en) 2006-10-10
AU7532501A (en) 2001-12-24
EP1297337B1 (en) 2011-01-19
US20040096924A1 (en) 2004-05-20
US20060051827A1 (en) 2006-03-09
JP2004503777A (en) 2004-02-05

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