WO2002000729A3 - Generation of libraries of antibodies in yeast and uses thereof - Google Patents

Generation of libraries of antibodies in yeast and uses thereof Download PDF

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Publication number
WO2002000729A3
WO2002000729A3 PCT/US2001/020542 US0120542W WO0200729A3 WO 2002000729 A3 WO2002000729 A3 WO 2002000729A3 US 0120542 W US0120542 W US 0120542W WO 0200729 A3 WO0200729 A3 WO 0200729A3
Authority
WO
WIPO (PCT)
Prior art keywords
tester
proteins
protein
target
library
Prior art date
Application number
PCT/US2001/020542
Other languages
French (fr)
Other versions
WO2002000729A2 (en
Inventor
Li Zhu
Shaobing Benjamin Hua
Original Assignee
Genetastix Corp
Li Zhu
Shaobing Benjamin Hua
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/603,658 external-priority patent/US6410246B1/en
Priority claimed from US09/603,663 external-priority patent/US6406863B1/en
Application filed by Genetastix Corp, Li Zhu, Shaobing Benjamin Hua filed Critical Genetastix Corp
Priority to EP01950579A priority Critical patent/EP1297124A2/en
Priority to AU2001271553A priority patent/AU2001271553A1/en
Priority to CA002411515A priority patent/CA2411515A1/en
Publication of WO2002000729A2 publication Critical patent/WO2002000729A2/en
Publication of WO2002000729A3 publication Critical patent/WO2002000729A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor

Abstract

Compositions, kits and methods are provided for generating highly diverse libraries of proteins such as antibodies via homologous recombination in vivo, and screening these libraries against protein, peptide and nucleic acid targets using a two-hybrid method in yeast. The method for screening a library of tester fusion proteins against a target protein or peptide comprises: expressing a library of tester proteins in yeast cells, the tester fusion protein comprising a first polypeptide subunit whose sequence varies within the library, a second polypeptide subunit whose sequence varies within the library independently of the first polypeptide, and a linker peptide which links the first and second polypeptide subunits; expressing one or more target fusion proteins in the yeast cells expressing the tester proteins, each of the target fusion proteins comprising a target peptide or protein; and selecting those yeast cells in which a reporter gene is expressed, the expression of the reporter gene being activated by binding of the tester fusion protein to the target fusion protein.
PCT/US2001/020542 2000-06-23 2001-06-25 Generation of libraries of antibodies in yeast and uses thereof WO2002000729A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP01950579A EP1297124A2 (en) 2000-06-23 2001-06-25 Generation of libraries of antibodies in yeast and uses thereof
AU2001271553A AU2001271553A1 (en) 2000-06-23 2001-06-25 High throughput generation and screening of fully human antibody repertoire in yeast
CA002411515A CA2411515A1 (en) 2000-06-23 2001-06-25 Generation of libraries of antibodies in yeast and uses thereof

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US60297300A 2000-06-23 2000-06-23
US60297200A 2000-06-23 2000-06-23
US09/603,658 US6410246B1 (en) 2000-06-23 2000-06-23 Highly diverse library of yeast expression vectors
US09/603,663 2000-06-23
US09/603,658 2000-06-23
US09/603,663 US6406863B1 (en) 2000-06-23 2000-06-23 High throughput generation and screening of fully human antibody repertoire in yeast
US09/602,373 US6410271B1 (en) 2000-06-23 2000-06-23 Generation of highly diverse library of expression vectors via homologous recombination in yeast
US09/602,972 2000-06-23
US09/602,373 2000-06-23

Publications (2)

Publication Number Publication Date
WO2002000729A2 WO2002000729A2 (en) 2002-01-03
WO2002000729A3 true WO2002000729A3 (en) 2002-06-13

Family

ID=24411088

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/020542 WO2002000729A2 (en) 2000-06-23 2001-06-25 Generation of libraries of antibodies in yeast and uses thereof

Country Status (2)

Country Link
US (2) US6410271B1 (en)
WO (1) WO2002000729A2 (en)

Families Citing this family (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6696251B1 (en) 1996-05-31 2004-02-24 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
US6759243B2 (en) * 1998-01-20 2004-07-06 Board Of Trustees Of The University Of Illinois High affinity TCR proteins and methods
US6610472B1 (en) * 2000-10-31 2003-08-26 Genetastix Corporation Assembly and screening of highly complex and fully human antibody repertoire in yeast
US7704964B2 (en) 2001-08-17 2010-04-27 Exothera L.L.C. Methods and compounds for the targeting of protein to exosomes
US20030059888A1 (en) * 2001-08-22 2003-03-27 Shengfeng Li Compositions and methods for generating antigen-binding units
EP1438400B1 (en) * 2001-10-01 2009-06-17 Dyax Corp. Multi-chain eukaryotic display vectors and uses thereof
US20050075298A1 (en) * 2002-01-31 2005-04-07 Wei Chen Methods and composition for delivering nucleic acids and/or proteins to the intestinal mucosa
US20040009937A1 (en) * 2002-01-31 2004-01-15 Wei Chen Methods and composition for delivering nucleic acids and/or proteins to the respiratory system
US20040043003A1 (en) * 2002-01-31 2004-03-04 Wei Chen Clinical grade vectors based on natural microflora for use in delivering therapeutic compositions
US8853362B2 (en) 2002-05-22 2014-10-07 Esbatech, An Alcon Biomedical Research Unit Llc Immunoglobulin frameworks which demonstrate enhanced stability in the intracellular environment and methods of identifying same
EP1932916A1 (en) * 2002-06-14 2008-06-18 Dyax Corporation Methods involving recombination of nucleic acid library members
ATE386810T1 (en) * 2002-06-14 2008-03-15 Dyax Corp RECOMBINATION OF NUCLEIC ACID LIBRARY MEMBERS
JP4748984B2 (en) * 2002-06-16 2011-08-17 ダイアックス、コープ Recombination of nucleic acid library members
US20040067532A1 (en) 2002-08-12 2004-04-08 Genetastix Corporation High throughput generation and affinity maturation of humanized antibody
JP4939926B2 (en) 2003-02-14 2012-05-30 アノシス・インコーポレーテッド Methods and compounds for generating antibodies and screening antibody repertoires
EP1613751A1 (en) 2003-04-11 2006-01-11 Esbatech AG Method for the construction of randomized gene sequence libraries in cells
US7709610B2 (en) 2003-05-08 2010-05-04 Facet Biotech Corporation Therapeutic use of anti-CS1 antibodies
US20050025763A1 (en) 2003-05-08 2005-02-03 Protein Design Laboratories, Inc. Therapeutic use of anti-CS1 antibodies
CA2525250A1 (en) * 2003-05-09 2004-11-25 Health Research Inc. Improved methods for protein interaction determination
US7973134B2 (en) 2004-07-07 2011-07-05 Cell Signaling Technology, Inc. Reagents for the detection of protein phosphorylation in anaplastic large cell lymphoma signaling pathways
US7935790B2 (en) 2004-10-04 2011-05-03 Cell Singaling Technology, Inc. Reagents for the detection of protein phosphorylation in T-cell receptor signaling pathways
US7807789B2 (en) 2004-12-21 2010-10-05 Cell Signaling Technology, Inc. Reagents for the detection of protein phosphorylation in EGFR-signaling pathways
WO2007027906A2 (en) 2005-08-31 2007-03-08 Cell Signaling Technology, Inc. Reagents for the detection of protein phosphorylation in leukemia signaling pathways
US7745393B2 (en) * 2005-11-03 2010-06-29 Jumi Shin Minimalist bZIP proteins and uses thereof
EP1951890A4 (en) 2005-11-16 2009-06-24 Ambrx Inc Methods and compositions comprising non-natural amino acids
JP2009520468A (en) 2005-12-23 2009-05-28 ヴィヴェンティア バイオテック インコーポレーティッド Fusion protein library production and screening methods, and uses thereof
US7939636B2 (en) 2006-08-11 2011-05-10 Cell Signaling Technology, Inc. Reagents for the detection of protein phosphorylation in c-Src signaling pathways
FR2910493B1 (en) * 2006-12-20 2012-12-14 Bio Modeling Systems Ou Bmsystems METHOD FOR THE RANDOM DIVERSIFICATION OF A GENETIC SEQUENCE FOR PRESERVING THE IDENTITY OF CERTAIN INTERNAL SEGMENTS OF THE GENETIC SEQUENCE
US20090081659A1 (en) 2007-03-07 2009-03-26 Cell Signaling Technology, Inc. Reagents for the detection of protein phosphorylation in carcinoma signaling pathways
EP1975184A3 (en) 2007-03-26 2008-11-26 Cell Signaling Technology, Inc. Serine or threonine phosphorylation sites
EP1983002A3 (en) 2007-04-19 2009-03-11 Peter Hornbeck Tyrosine phosphorylation sites and antibodies specific for them
US7977462B2 (en) 2007-04-19 2011-07-12 Cell Signaling Technology, Inc. Tyrosine phosphorylation sites
EP1983003A3 (en) 2007-04-19 2009-03-11 Peter Hornbeck Tyrosine phosphorylation sites and antibodies specific for them
US20090053831A1 (en) 2007-05-01 2009-02-26 Cell Signaling Technology, Inc. Tyrosine phosphorylation sites
EP2193146B1 (en) 2007-09-14 2016-05-25 Adimab, LLC Rationally designed, synthetic antibody libraries and uses therefor
US8877688B2 (en) 2007-09-14 2014-11-04 Adimab, Llc Rationally designed, synthetic antibody libraries and uses therefor
EP2062920A3 (en) 2007-11-21 2009-06-17 Peter Hornbeck Protein phosphorylation by basophilic serine/threonine kinases in insulin signalling pathways
US20110070601A1 (en) * 2008-01-23 2011-03-24 Rigshospitalet Classification of individuals suffering from cardiovascular diseases according to survival prognoses as found by measuring the levels of biomarker ykl-40
EP2274437B1 (en) 2008-04-10 2015-12-23 Cell Signaling Technology, Inc. Compositions and methods for detecting egfr mutations in cancer
CN101343323B (en) * 2008-08-29 2011-02-16 浙江大学 Anti-interleukins-8 antibody
EP4219805A1 (en) 2010-07-16 2023-08-02 Adimab, LLC Antibody libraries
US9915665B2 (en) 2012-12-28 2018-03-13 Abbvie Inc. High-throughput system and method for identifying antibodies having specific antigen binding activities
US9458244B2 (en) 2012-12-28 2016-10-04 Abbvie Inc. Single chain multivalent binding protein compositions and methods
EP3564379A1 (en) 2013-09-13 2019-11-06 California Institute of Technology Selective recovery
EP3077508B1 (en) 2013-12-04 2018-11-14 AxioMx, Inc. Methods of utilizing recombination for the identification of binding moieties
US10816554B2 (en) 2014-04-04 2020-10-27 The Board Of Trustees Of The Leland Stanford Junior University Ligand discovery for T cell receptors
SG11201804713VA (en) 2015-12-11 2018-07-30 California Inst Of Techn TARGETING PEPTIDES FOR DIRECTING ADENO-ASSOCIATED VIRUSES (AAVs)
AU2018359218B2 (en) 2017-11-01 2021-11-11 Nantbio, Inc. IL8 blocking EMT pathway and overcoming cancer stem cells
US20200370070A1 (en) * 2017-11-17 2020-11-26 The Johns Hopkins University Compositions and methods for efficient genome editing
WO2020068990A1 (en) 2018-09-26 2020-04-02 California Institute Of Technology Adeno-associated virus compositions for targeted gene therapy
CN113215145B (en) * 2021-05-08 2022-07-08 河南大学 Short fragment cloning method independent of PCR reaction

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999028502A1 (en) * 1997-11-28 1999-06-10 Invitrogen Corporation Single chain monoclonal antibody fusion reagents that regulate transcription in vivo

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4946778A (en) 1987-09-21 1990-08-07 Genex Corporation Single polypeptide chain binding molecules
US5283173A (en) 1990-01-24 1994-02-01 The Research Foundation Of State University Of New York System to detect protein-protein interactions
US5962255A (en) * 1992-03-24 1999-10-05 Cambridge Antibody Technology Limited Methods for producing recombinant vectors
DE69334095T2 (en) 1992-07-17 2007-04-12 Dana-Farber Cancer Institute, Boston Method for intracellular binding of targeted molecules
US5922545A (en) 1993-10-29 1999-07-13 Affymax Technologies N.V. In vitro peptide and antibody display libraries
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5525490A (en) 1994-03-29 1996-06-11 Onyx Pharmaceuticals, Inc. Reverse two-hybrid method
US5888773A (en) 1994-08-17 1999-03-30 The United States Of America As Represented By The Department Of Health And Human Services Method of producing single-chain Fv molecules
US5763733A (en) 1994-10-13 1998-06-09 Enzon, Inc. Antigen-binding fusion proteins
GB9504344D0 (en) 1995-03-03 1995-04-19 Unilever Plc Antibody fragment production
WO1997041255A1 (en) 1996-04-26 1997-11-06 Massachusetts Institute Of Technology Three-hybrid screening assay
US5955275A (en) 1997-02-14 1999-09-21 Arcaris, Inc. Methods for identifying nucleic acid sequences encoding agents that affect cellular phenotypes
US5869250A (en) 1996-12-02 1999-02-09 The University Of North Carolina At Chapel Hill Method for the identification of peptides that recognize specific DNA sequences
AU7266898A (en) * 1997-04-30 1998-11-24 Enzon, Inc. Single-chain antigen-binding proteins capable of glycosylation, production and uses thereof
US20020029391A1 (en) 1998-04-15 2002-03-07 Claude Geoffrey Davis Epitope-driven human antibody production and gene expression profiling

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999028502A1 (en) * 1997-11-28 1999-06-10 Invitrogen Corporation Single chain monoclonal antibody fusion reagents that regulate transcription in vivo

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BENDIXEN C ET AL: "A yeast mating selection scheme for detection of protein-protein interaction", NUCLEIC ACIDS RES., vol. 22, no. 9, 1994, pages 1778 - 1779, XP002004038 *
HOLTZ, A. E. ET AL.: "Yeast Hybrid Technologies: Chapter 5 (Mating-Based Yeast Two-Hybrid Screening)", 2000, EATON PUBLISHING, NATICK, MA, XP001065214 *
VISINTIN MICHELA ET AL: "Selection of antibodies for intracellular function using a two-hybrid in vivo system", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, NATIONAL ACADEMY OF SCIENCE, WASHINGTON, DC, US, vol. 96, no. 21, 12 October 1999 (1999-10-12), pages 11723 - 11728, XP002143442, ISSN: 0027-8424 *
WANG PETER L: "Creating hybrid genes by homologous recombination.", DISEASE MARKERS, vol. 16, no. 1-2, 2000, Meeting on New Horizons for Phage Antibody Display Technology;Hull, England, UK; April 11-13, 2000, pages 3 - 13, XP001064283, ISSN: 0278-0240 *

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Publication number Publication date
US6410271B1 (en) 2002-06-25
US20030165990A1 (en) 2003-09-04
WO2002000729A2 (en) 2002-01-03

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