WO2002010747A3 - Method and apparatus for patch-clamp measurements on cells - Google Patents

Method and apparatus for patch-clamp measurements on cells Download PDF

Info

Publication number
WO2002010747A3
WO2002010747A3 PCT/EP2001/008831 EP0108831W WO0210747A3 WO 2002010747 A3 WO2002010747 A3 WO 2002010747A3 EP 0108831 W EP0108831 W EP 0108831W WO 0210747 A3 WO0210747 A3 WO 0210747A3
Authority
WO
WIPO (PCT)
Prior art keywords
capillary
cell
patch
cells
clamp measurements
Prior art date
Application number
PCT/EP2001/008831
Other languages
French (fr)
Other versions
WO2002010747A2 (en
Inventor
Albrecht Lepple-Wienhues
Original Assignee
Flyion Gmbh
Lepple Wienhues Albrecht
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Flyion Gmbh, Lepple Wienhues Albrecht filed Critical Flyion Gmbh
Priority to AU8204801A priority Critical patent/AU8204801A/en
Priority to DE60120396T priority patent/DE60120396T2/en
Priority to JP2002516623A priority patent/JP2004518109A/en
Priority to US10/110,751 priority patent/US7611861B2/en
Priority to CA002417796A priority patent/CA2417796A1/en
Priority to EP01960595A priority patent/EP1283985B1/en
Publication of WO2002010747A2 publication Critical patent/WO2002010747A2/en
Publication of WO2002010747A3 publication Critical patent/WO2002010747A3/en
Priority to HK04104902A priority patent/HK1061889A1/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48728—Investigating individual cells, e.g. by patch clamp, voltage clamp

Abstract

The invention shows a method for patch-clamp experiments on cells or similar structures, where at least one cell is inserted into the lumen of a capillary and is positioned inside the capillary so, that a sufficiently tight seal with a resistance exceeding 1, preferably 10 GigaOhm develops between cell membrane and inner surface of the capillary. Along its length said capillary has at least at one position a smaller inner diameter than the outer diameter of said cell. Preferably the cell is inserted and positioned in the capillary by pressure, suction, sedimentation or centrifugation of a suspension or solution containing said cell. An apparatus for performing experiments using this method is described.
PCT/EP2001/008831 2000-07-31 2001-07-31 Method and apparatus for patch-clamp measurements on cells WO2002010747A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
AU8204801A AU8204801A (en) 2000-07-31 2001-07-31 Method and apparatus for patch-clamp measurements on cells
DE60120396T DE60120396T2 (en) 2000-07-31 2001-07-31 METHOD AND DEVICE FOR PATCH CLAMP MEASUREMENTS ON CELLS
JP2002516623A JP2004518109A (en) 2000-07-31 2001-07-31 Method and apparatus for patch-clamp measurement on cells
US10/110,751 US7611861B2 (en) 2000-07-31 2001-07-31 Method and apparatus for patch-clamp measurements on cells
CA002417796A CA2417796A1 (en) 2000-07-31 2001-07-31 Method and apparatus for patch-clamp measurements on cells
EP01960595A EP1283985B1 (en) 2000-07-31 2001-07-31 Method and apparatus for patch-clamp measurements on cells
HK04104902A HK1061889A1 (en) 2000-07-31 2004-07-06 Method and apparatus for with the measurements on cells technique

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP00116515.8 2000-07-31
EP00116515A EP1178315A1 (en) 2000-07-31 2000-07-31 Method and apparatus for examining cells using the patch clamp method

Publications (2)

Publication Number Publication Date
WO2002010747A2 WO2002010747A2 (en) 2002-02-07
WO2002010747A3 true WO2002010747A3 (en) 2002-04-18

Family

ID=8169404

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/008831 WO2002010747A2 (en) 2000-07-31 2001-07-31 Method and apparatus for patch-clamp measurements on cells

Country Status (12)

Country Link
US (1) US7611861B2 (en)
EP (2) EP1178315A1 (en)
JP (1) JP2004518109A (en)
CN (1) CN1327222C (en)
AT (1) ATE329261T1 (en)
AU (1) AU8204801A (en)
CA (1) CA2417796A1 (en)
DE (1) DE60120396T2 (en)
DK (1) DK1283985T3 (en)
ES (1) ES2266235T3 (en)
HK (1) HK1061889A1 (en)
WO (1) WO2002010747A2 (en)

Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU9786798A (en) * 1997-10-10 1999-05-03 Biosepra Inc. Aligned multiwell multiplate stack and method for processing biological/chemicalsamples using the same
DE59801410D1 (en) 1997-12-17 2001-10-11 Ecole Polytech POSITIONING AND ELECTROPHYSIOLOGICAL CHARACTERIZATION OF INDIVIDUAL CELLS AND RECONSTRUCTED MEMBRANE SYSTEMS ON MICROSTRUCTURED CARRIERS
US7244349B2 (en) * 1997-12-17 2007-07-17 Molecular Devices Corporation Multiaperture sample positioning and analysis system
US20020144905A1 (en) * 1997-12-17 2002-10-10 Christian Schmidt Sample positioning and analysis system
GB9930719D0 (en) 1999-12-24 2000-02-16 Central Research Lab Ltd Apparatus for and method of making electrical measurements on an object in a m edium
US7067046B2 (en) * 2000-08-04 2006-06-27 Essen Instruments, Inc. System for rapid chemical activation in high-throughput electrophysiological measurements
DE60217393T2 (en) * 2001-11-27 2007-10-18 Cellectricon Ab METHOD FOR THE COMBINED PARALLEL INTRODUCTION OF AGENTS AND ELECTROPORATION FOR CELL STRUCTURES AND USE THEREOF
AU2003205132A1 (en) * 2002-02-12 2003-09-04 Celectricon AB Systems and methods for rapidly changing the solution environment around sensors
ES2208082B1 (en) * 2002-05-29 2005-09-16 Jose Luis Bardasano Rubio DEVICE FOR MEASURING THE POTENTIAL OF TRANSMEMBRANE ACTION IN CELLULAR PREPARATIONS UNDER THE ACTION OF ELECTROMAGNETIC FIELDS OF VARIABLE FREQUENCY AND INTENSITY.
WO2004018690A1 (en) * 2002-08-21 2004-03-04 Cellectricon Ab System and method for obtaining and maintaining high resistance seals in patch clamp recordings
AU2003278461A1 (en) 2002-10-16 2004-05-04 Cellectricon Ab Nanoelectrodes and nanotips for recording transmembrane currents in a plurality of cells
FR2866958B1 (en) * 2004-02-26 2006-08-04 Commissariat Energie Atomique METHOD AND DEVICE FOR CONTROLLING THE POSITIONING OF A BIOLOGICAL ELEMENT ON A SUPPORT
US8058056B2 (en) * 2004-03-12 2011-11-15 The Regents Of The University Of California Method and apparatus for integrated cell handling and measurements
US20060134772A1 (en) * 2004-11-18 2006-06-22 The Regents Of The University Of California System for locating cells and for cellular analysis
WO2007108773A1 (en) * 2006-03-23 2007-09-27 Agengy For Science, Technology And Research Device for analyzing the status of a particle
DE102006014513A1 (en) * 2006-03-23 2007-09-27 Flyion Gmbh Glass pipette or glass capillary for patch clamp experiments
US8293524B2 (en) * 2006-03-31 2012-10-23 Fluxion Biosciences Inc. Methods and apparatus for the manipulation of particle suspensions and testing thereof
US8361094B2 (en) 2006-06-30 2013-01-29 Atheromed, Inc. Atherectomy devices and methods
CA2656594A1 (en) 2006-06-30 2008-01-10 Atheromed, Inc. Atherectomy devices and methods
US20090018566A1 (en) 2006-06-30 2009-01-15 Artheromed, Inc. Atherectomy devices, systems, and methods
US20080004645A1 (en) 2006-06-30 2008-01-03 Atheromed, Inc. Atherectomy devices and methods
US8070762B2 (en) 2007-10-22 2011-12-06 Atheromed Inc. Atherectomy devices and methods
EP2302375B1 (en) * 2009-09-29 2012-09-12 Karlsruher Institut für Technologie Method and device for recording the current voltage curve of a cell
CN102380357B (en) * 2010-08-31 2014-07-02 北京大学 Capillary tube with fixed phase formed by culturing cell layer through adhering cell layer on inner wall of capillary tube and manufacturing method and application thereof
DE102014203280B4 (en) 2014-02-24 2015-12-17 Cytocentrics Bioscience Gmbh Device for determining measured quantities on membranes
CN106033069B (en) * 2015-03-10 2019-05-28 中国科学院生物物理研究所 Single Vesicle fusion and recycling and its patch-clamp detection device coupled with calcium channel
WO2016161081A1 (en) 2015-04-03 2016-10-06 Fluxion Biosciences, Inc. Molecular characterization of single cells and cell populations for non-invasive diagnostics
US10830758B2 (en) 2015-08-09 2020-11-10 Georgia Tech Research Corporation Systems and methods enabling patch-clamp re-use
CN108489867B (en) * 2018-04-19 2020-01-31 中国科学院地球化学研究所 method for determining cell transport capacity of plant leaf
WO2020104348A1 (en) * 2018-11-20 2020-05-28 F. Hoffmann-La Roche Ag Method for seeding cells on a sensor surface
CN109374875A (en) * 2018-11-20 2019-02-22 中国科学院生物物理研究所 Low noise constant temperature gravity perfusion patch clamp
CN112345616B (en) * 2020-09-14 2022-03-11 中国科学院生物物理研究所 High-precision electrochemical detection method suitable for detecting biological active substances at single cell and sub-cell level
CN112280667A (en) * 2020-10-20 2021-01-29 深圳麦科田生物医疗技术有限公司 Single cell extraction method
US11304723B1 (en) 2020-12-17 2022-04-19 Avantec Vascular Corporation Atherectomy devices that are self-driving with controlled deflection
CN113281441A (en) * 2021-05-19 2021-08-20 中国科学技术大学 Single cell organelle mass spectrum detection method and detection device
CN113406316B (en) * 2021-06-17 2023-08-08 重庆医科大学附属儿童医院 Electrophysiology patch clamp perfusion device
EP4231011A1 (en) * 2022-02-16 2023-08-23 Luigs & Neumann Feinmechanik und Elektrotechnik GmbH Devices and methods for patch clamp measurement techniques

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3714565A (en) * 1971-03-26 1973-01-30 Coulter Electronics Electronic particle analyzing apparatus with improved aperture tube
US4055799A (en) * 1975-01-23 1977-10-25 Kernforschungsanlage Julich Gesellschaft Mit Beschrankter Haftung Method of and device for measuring elastic and di-electric properties of the diaphragm of living cells
US4253058A (en) * 1977-11-11 1981-02-24 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. Device for measuring certain properties of particles suspended in a particle suspension
DE3626600A1 (en) * 1985-09-10 1987-03-19 Schwerionenforsch Gmbh Apparatus for determining properties of particles suspended in liquids
WO1997017426A1 (en) * 1995-11-08 1997-05-15 Trustees Of Boston University Cellular physiology workstations for automated data acquisition and perfusion control
DE19712309A1 (en) * 1996-11-16 1998-05-20 Nmi Univ Tuebingen Microelement arrangement, method for contacting cells in a liquid environment and method for producing a microelement arrangement
DE19744649A1 (en) * 1997-10-09 1999-04-15 Fraunhofer Ges Forschung Patch clamp cell measurement apparatus, has an array of micro dishes containing cells, and an assembly of micro pipettes with relative movements between them

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CH508539A (en) * 1969-06-28 1971-06-15 Coster Tecnologie Speciali Spa Apparatus for taking valves equipped with a suction tube for aerosol containers from a tank and conveying them to a collection guide
JPS6089238U (en) * 1983-11-25 1985-06-19 株式会社成茂科学器械研究所 Microelectrode manufacturing device for patch clamp
WO1998022819A1 (en) * 1996-11-16 1998-05-28 Nmi Naturwissenschaftliches Und Medizinisches Institut An Der Universität Tübingen In Reutlingen Stiftung Bürgerlichen Rechts Array of microelements, method of contacting cells in a liquid environment and method for the production of an array of microelements
DE59801410D1 (en) * 1997-12-17 2001-10-11 Ecole Polytech POSITIONING AND ELECTROPHYSIOLOGICAL CHARACTERIZATION OF INDIVIDUAL CELLS AND RECONSTRUCTED MEMBRANE SYSTEMS ON MICROSTRUCTURED CARRIERS
EP1061975B1 (en) * 1998-03-13 2004-02-11 Becton, Dickinson and Company Method for assembling and packaging medical devices
JPH11299496A (en) * 1998-04-24 1999-11-02 Hitachi Ltd Vessel for oocyte and measuring device using the same
GB9812783D0 (en) 1998-06-12 1998-08-12 Cenes Ltd High throuoghput screen
DE69907249T2 (en) * 1998-10-08 2004-01-29 Astrazeneca Ab MICROFABRICATED CELL INJECTOR
DE19948473A1 (en) * 1999-10-08 2001-04-12 Nmi Univ Tuebingen Method and device for measuring cells in a liquid environment
GB9930719D0 (en) * 1999-12-24 2000-02-16 Central Research Lab Ltd Apparatus for and method of making electrical measurements on an object in a m edium

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3714565A (en) * 1971-03-26 1973-01-30 Coulter Electronics Electronic particle analyzing apparatus with improved aperture tube
US4055799A (en) * 1975-01-23 1977-10-25 Kernforschungsanlage Julich Gesellschaft Mit Beschrankter Haftung Method of and device for measuring elastic and di-electric properties of the diaphragm of living cells
US4253058A (en) * 1977-11-11 1981-02-24 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. Device for measuring certain properties of particles suspended in a particle suspension
DE3626600A1 (en) * 1985-09-10 1987-03-19 Schwerionenforsch Gmbh Apparatus for determining properties of particles suspended in liquids
WO1997017426A1 (en) * 1995-11-08 1997-05-15 Trustees Of Boston University Cellular physiology workstations for automated data acquisition and perfusion control
DE19712309A1 (en) * 1996-11-16 1998-05-20 Nmi Univ Tuebingen Microelement arrangement, method for contacting cells in a liquid environment and method for producing a microelement arrangement
DE19744649A1 (en) * 1997-10-09 1999-04-15 Fraunhofer Ges Forschung Patch clamp cell measurement apparatus, has an array of micro dishes containing cells, and an assembly of micro pipettes with relative movements between them

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
HAMILL O P ET AL: "IMPROVED PATCH-CLAMP TECHNIQUES FOR HIGH-RESOLUTION CURRENT RECORDING FROM CELLS AND CELL-FREE MEMBRANE PATCHES", PFLUEGERS ARCHIV,DE,SPRINGER VERLAG, BERLIN, vol. 391, 1981, pages 85 - 100, XP000196663, ISSN: 0031-6768 *

Also Published As

Publication number Publication date
ATE329261T1 (en) 2006-06-15
EP1283985A2 (en) 2003-02-19
ES2266235T3 (en) 2007-03-01
US7611861B2 (en) 2009-11-03
CN1327222C (en) 2007-07-18
DE60120396D1 (en) 2006-07-20
HK1061889A1 (en) 2004-10-08
EP1178315A1 (en) 2002-02-06
EP1283985B1 (en) 2006-06-07
AU8204801A (en) 2002-02-13
WO2002010747A2 (en) 2002-02-07
CA2417796A1 (en) 2002-02-07
US20030022268A1 (en) 2003-01-30
CN1466681A (en) 2004-01-07
DK1283985T3 (en) 2006-10-09
JP2004518109A (en) 2004-06-17
DE60120396T2 (en) 2007-06-06

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