WO2002023185A3 - Devices and methods for direct electronic readout of biomolecular interactions - Google Patents

Devices and methods for direct electronic readout of biomolecular interactions Download PDF

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Publication number
WO2002023185A3
WO2002023185A3 PCT/US2001/028821 US0128821W WO0223185A3 WO 2002023185 A3 WO2002023185 A3 WO 2002023185A3 US 0128821 W US0128821 W US 0128821W WO 0223185 A3 WO0223185 A3 WO 0223185A3
Authority
WO
WIPO (PCT)
Prior art keywords
devices
methods
electrically conductive
probe
type
Prior art date
Application number
PCT/US2001/028821
Other languages
French (fr)
Other versions
WO2002023185A2 (en
Inventor
John J Schwartz
Joseph Jacobson
Original Assignee
Engeneos Inc
John J Schwartz
Joseph Jacobson
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Engeneos Inc, John J Schwartz, Joseph Jacobson filed Critical Engeneos Inc
Priority to AU2001290954A priority Critical patent/AU2001290954A1/en
Publication of WO2002023185A2 publication Critical patent/WO2002023185A2/en
Publication of WO2002023185A3 publication Critical patent/WO2002023185A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
    • G01N27/3276—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction being a hybridisation with immobilised receptors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Abstract

Disclosed herein are methods and devices for direct electronic readout of biomolecular interactions. The devices of the invention include substrates having a number of electrically conductive sites. The sites are arranged in a spatially-addressable array and are electrically isolated from each other. The devices also include a number of types of probes, each type of probe having a hybridization moiety capable of recognising and hybridizing to a different nucleic acid sequence. Generally, each site is in electrically conductive contact with one type of probe. Similarly, each type of probe is in electrically conductive contact with at least one electrically conductive site. The devices and methods of the invention permit detection of genetic lesions indicative of disease. Also, the devices and methods permit measurement of nucleic acid concentrations useful for predictive medicine as well as therapeutic drug monitoring, for example.
PCT/US2001/028821 2000-09-15 2001-09-14 Devices and methods for direct electronic readout of biomolecular interactions WO2002023185A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001290954A AU2001290954A1 (en) 2000-09-15 2001-09-14 Devices and methods for direct electronic readout of biomolecular interactions

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US23267200P 2000-09-15 2000-09-15
US60/232,672 2000-09-15

Publications (2)

Publication Number Publication Date
WO2002023185A2 WO2002023185A2 (en) 2002-03-21
WO2002023185A3 true WO2002023185A3 (en) 2004-09-02

Family

ID=22874075

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/028821 WO2002023185A2 (en) 2000-09-15 2001-09-14 Devices and methods for direct electronic readout of biomolecular interactions

Country Status (2)

Country Link
AU (1) AU2001290954A1 (en)
WO (1) WO2002023185A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ITPD20050365A1 (en) * 2005-12-14 2007-06-15 Univ Degli Studi Trieste METHOD AND SYSTEM OF RECOGNITION OF GENETIC PATTERNS
JP6455438B2 (en) 2012-12-13 2019-01-23 ワン バイオームド ピーティーイー リミテッド Label-free method for isolation and analysis of nucleic acids in solid phase devices
KR102533531B1 (en) * 2016-03-30 2023-05-18 와카스 칼리드 Sensors based on nanostructured arrays for electrochemical sensing, capacitive sensing and field emission sensing

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5653939A (en) * 1991-11-19 1997-08-05 Massachusetts Institute Of Technology Optical and electrical methods and apparatus for molecule detection
JPH10146183A (en) * 1996-09-19 1998-06-02 Toshiba Corp Electrode, detection device and sensor
US5972692A (en) * 1990-09-28 1999-10-26 Kabushiki Kaisha Toshiba Gene detection method
WO1999067628A1 (en) * 1998-06-24 1999-12-29 Therasense, Inc. Multi-sensor array for electrochemical recognition of nucleotide sequences and methods
WO2001044501A2 (en) * 1999-12-13 2001-06-21 november Aktiengesellschaft Gesellschaft für Molekulare Medizin Method and device for detecting and quantifying biomolecules

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5972692A (en) * 1990-09-28 1999-10-26 Kabushiki Kaisha Toshiba Gene detection method
US5653939A (en) * 1991-11-19 1997-08-05 Massachusetts Institute Of Technology Optical and electrical methods and apparatus for molecule detection
JPH10146183A (en) * 1996-09-19 1998-06-02 Toshiba Corp Electrode, detection device and sensor
WO1999067628A1 (en) * 1998-06-24 1999-12-29 Therasense, Inc. Multi-sensor array for electrochemical recognition of nucleotide sequences and methods
WO2001044501A2 (en) * 1999-12-13 2001-06-21 november Aktiengesellschaft Gesellschaft für Molekulare Medizin Method and device for detecting and quantifying biomolecules

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
PATENT ABSTRACTS OF JAPAN vol. 1998, no. 11 30 September 1998 (1998-09-30) *

Also Published As

Publication number Publication date
AU2001290954A1 (en) 2002-03-26
WO2002023185A2 (en) 2002-03-21

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