WO2002029003A2 - Massive parallel method for decoding dna and rna - Google Patents
Massive parallel method for decoding dna and rna Download PDFInfo
- Publication number
- WO2002029003A2 WO2002029003A2 PCT/US2001/031243 US0131243W WO0229003A2 WO 2002029003 A2 WO2002029003 A2 WO 2002029003A2 US 0131243 W US0131243 W US 0131243W WO 0229003 A2 WO0229003 A2 WO 0229003A2
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- WIPO (PCT)
- Prior art keywords
- group
- analogue
- nucleotide
- solid surface
- dna
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/06—Pyrimidine radicals
- C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
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- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/14—Pyrrolo-pyrimidine radicals
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- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6872—Methods for sequencing involving mass spectrometry
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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- C07—ORGANIC CHEMISTRY
- C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
- C07B2200/00—Indexing scheme relating to specific properties of organic compounds
- C07B2200/11—Compounds covalently bound to a solid support
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- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/117—Modifications characterised by incorporating modified base
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety
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- C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
- C12Q2535/101—Sanger sequencing method, i.e. oligonucleotide sequencing using primer elongation and dideoxynucleotides as chain terminators
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
- C12Q2535/122—Massive parallel sequencing
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- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
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- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/50—Detection characterised by immobilisation to a surface
- C12Q2565/501—Detection characterised by immobilisation to a surface being an array of oligonucleotides
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- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
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- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
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- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Abstract
Description
Claims
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP01977533A EP1337541B1 (en) | 2000-10-06 | 2001-10-05 | Massive parallel method for decoding DNA and RNA |
CA2425112A CA2425112C (en) | 2000-10-06 | 2001-10-05 | Massive parallel method for decoding dna and rna |
DE60127162T DE60127162T2 (en) | 2000-10-06 | 2001-10-05 | Massive parallel method for the decoding of DNA and RNA |
AU2001296645A AU2001296645A1 (en) | 2000-10-06 | 2001-10-05 | Massive parallel method for decoding dna and rna |
EP15195765.1A EP3034627B1 (en) | 2000-10-06 | 2001-10-05 | Massive parallel method for decoding dna and rna |
JP2002532574A JP2004510433A (en) | 2000-10-06 | 2001-10-05 | Massively parallel methods for decoding DNA and RNA |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US68467000A | 2000-10-06 | 2000-10-06 | |
US09/684,670 | 2000-10-06 | ||
US30089401P | 2001-06-26 | 2001-06-26 | |
US60/300,894 | 2001-06-26 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2002029003A2 true WO2002029003A2 (en) | 2002-04-11 |
WO2002029003A3 WO2002029003A3 (en) | 2002-07-18 |
Family
ID=26972030
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2001/031243 WO2002029003A2 (en) | 2000-10-06 | 2001-10-05 | Massive parallel method for decoding dna and rna |
Country Status (9)
Country | Link |
---|---|
US (25) | US6664079B2 (en) |
EP (3) | EP3034627B1 (en) |
JP (1) | JP2004510433A (en) |
AT (1) | ATE356222T1 (en) |
AU (1) | AU2001296645A1 (en) |
CA (1) | CA2425112C (en) |
DE (2) | DE20122767U1 (en) |
HK (1) | HK1227441A1 (en) |
WO (1) | WO2002029003A2 (en) |
Cited By (84)
Publication number | Priority date | Publication date | Assignee | Title |
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US6627748B1 (en) | 2000-09-11 | 2003-09-30 | The Trustees Of Columbia University In The City Of New York | Combinatorial fluorescence energy transfer tags and their applications for multiplex genetic analyses |
WO2003087839A1 (en) * | 2002-04-04 | 2003-10-23 | Xzillion Gmbh & Co. Kg | Method for characterising analytes |
US6664079B2 (en) | 2000-10-06 | 2003-12-16 | The Trustees Of Columbia University In The City Of New York | Massive parallel method for decoding DNA and RNA |
WO2004018493A1 (en) * | 2002-08-23 | 2004-03-04 | Solexa Limited | Labelled nucleotides |
WO2004018497A2 (en) | 2002-08-23 | 2004-03-04 | Solexa Limited | Modified nucleotides for polynucleotide sequencing |
EP1552007A1 (en) * | 2002-07-12 | 2005-07-13 | The Trustees of Columbia University in the City of New York | Multiplex genotyping using solid phase capturable dideoxynucleotides and mass spectrometry |
WO2006017208A1 (en) * | 2004-07-12 | 2006-02-16 | Applera Corporation | Mass tags for quantitative analyses |
US7057026B2 (en) | 2001-12-04 | 2006-06-06 | Solexa Limited | Labelled nucleotides |
EP1816130A1 (en) * | 2004-11-08 | 2007-08-08 | Riken | Novel nucleoside or nucleotide derivative and use thereof |
WO2008037568A2 (en) * | 2006-09-04 | 2008-04-03 | Quiatech Ab | Reversible terminators for efficient sequencing by synthesis |
US7405281B2 (en) | 2005-09-29 | 2008-07-29 | Pacific Biosciences Of California, Inc. | Fluorescent nucleotide analogs and uses therefor |
US7414116B2 (en) | 2002-08-23 | 2008-08-19 | Illumina Cambridge Limited | Labelled nucleotides |
WO2008101024A3 (en) * | 2007-02-13 | 2008-10-09 | Invitrogen Corp | Labeling and detection of nucleic acids |
US7622279B2 (en) | 2004-03-03 | 2009-11-24 | The Trustees Of Columbia University In The City Of New York | Photocleavable fluorescent nucleotides for DNA sequencing on chip constructed by site-specific coupling chemistry |
EP2125856A2 (en) * | 2006-12-05 | 2009-12-02 | Lasergen, Inc. | Photocleavable labeled nucleotides and nucleosides and labeled nucleotides and nucleosides and methods for their use in dna sequencing |
WO2009151921A1 (en) | 2008-05-27 | 2009-12-17 | Trilink Biotechnologies | Chemically modified nucleoside 5'-triphosphates for thermally initiated amplification of nucleic acid |
US7772384B2 (en) | 2001-12-04 | 2010-08-10 | Illumina Cambridge Limited | Labelled nucleotides |
US7883869B2 (en) | 2006-12-01 | 2011-02-08 | The Trustees Of Columbia University In The City Of New York | Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators |
GB2446084B (en) * | 2005-10-31 | 2011-03-02 | Univ Columbia | Synthesis of four color 3-o-allyl modified photocleavable fluorescent nucleotides and related methods |
GB2446083B (en) * | 2005-10-31 | 2011-03-02 | Univ Columbia | Chemically cleavable 3'-0-allyl-dntp-allyl-fluorophore fluorescent nucleotide analogues and related methods |
US7910335B2 (en) | 2005-10-27 | 2011-03-22 | President And Fellows Of Harvard College | Methods and compositions for labeling nucleic acids |
US8133515B2 (en) | 2007-11-21 | 2012-03-13 | University Of Georgia Research Foundation, Inc. | Alkynes and methods of reacting alkynes with 1,3-dipole-functional compounds |
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