WO2002031203A3 - High throughput or capillary-based screening for a bioactivity or biomolecule - Google Patents

High throughput or capillary-based screening for a bioactivity or biomolecule Download PDF

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Publication number
WO2002031203A3
WO2002031203A3 PCT/US2001/031806 US0131806W WO0231203A3 WO 2002031203 A3 WO2002031203 A3 WO 2002031203A3 US 0131806 W US0131806 W US 0131806W WO 0231203 A3 WO0231203 A3 WO 0231203A3
Authority
WO
WIPO (PCT)
Prior art keywords
organisms
based screening
mixed population
library
devices
Prior art date
Application number
PCT/US2001/031806
Other languages
French (fr)
Other versions
WO2002031203A9 (en
WO2002031203A2 (en
Inventor
Jay M Short
Martin Keller
William Michael Lafferty
Original Assignee
Diversa Corp
Jay M Short
Martin Keller
William Michael Lafferty
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/738,871 external-priority patent/US20010041333A1/en
Priority claimed from US09/790,321 external-priority patent/US20020048809A1/en
Priority claimed from US09/894,956 external-priority patent/US20020015997A1/en
Priority to CA002393374A priority Critical patent/CA2393374A1/en
Priority to JP2002534567A priority patent/JP2005501217A/en
Priority to IL15002001A priority patent/IL150020A0/en
Application filed by Diversa Corp, Jay M Short, Martin Keller, William Michael Lafferty filed Critical Diversa Corp
Priority to EP01979708A priority patent/EP1364052A2/en
Priority to MXPA02005717A priority patent/MXPA02005717A/en
Priority to AU11642/02A priority patent/AU1164202A/en
Publication of WO2002031203A2 publication Critical patent/WO2002031203A2/en
Publication of WO2002031203A9 publication Critical patent/WO2002031203A9/en
Publication of WO2002031203A3 publication Critical patent/WO2002031203A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Abstract

Provided is a method of screening or enriching a sample containing polynucleotides from a mixed population of organisms. The method includes creating a DNA library from a plurality of nucleic acid sequences of a mixed population of organisms and separating clones containing a polynucleotide sequence of interest on an analyzer detects a detectable molecule on a probe or bioactive substrate. The analyzer includes FACS devices, SQUID devices and MSC devices. The separated or enrich library can then be further process by activity based screening or sequence based screening. In addition, the enriched sequence can be compared to a database and to identify sequences in the database which have homology to a clone in the library thereby obtaining a nucleic acid profile of the mixed population of organisms.
PCT/US2001/031806 2000-10-10 2001-10-10 High throughput or capillary-based screening for a bioactivity or biomolecule WO2002031203A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
AU11642/02A AU1164202A (en) 2000-10-10 2001-10-10 High throughput or capillary-based screening for a bioactivity or biomolecule
MXPA02005717A MXPA02005717A (en) 2000-10-10 2001-10-10 High throughput or capillary-based screening for a bioactivity or biomolecule.
EP01979708A EP1364052A2 (en) 2000-10-10 2001-10-10 High throughput or capillary-based screening for a bioactivity or biomolecule
JP2002534567A JP2005501217A (en) 2000-10-10 2001-10-10 High-throughput or capillary-based screening for bioactivity or biomolecules
IL15002001A IL150020A0 (en) 2000-10-10 2001-10-10 High throughput or capillary-based screening for a bioactivity or biomolecule
CA002393374A CA2393374A1 (en) 2000-10-10 2001-10-10 High throughput or capillary-based screening for a bioactivity or biomolecule

Applications Claiming Priority (10)

Application Number Priority Date Filing Date Title
US68543200A 2000-10-10 2000-10-10
US09/685,432 2000-10-10
US09/738,871 US20010041333A1 (en) 1997-06-16 2000-12-15 High throughput screening for a bioactivity or biomolecule
US09/738,871 2000-12-15
US09/790,321 2001-02-21
US09/790,321 US20020048809A1 (en) 1997-06-16 2001-02-21 Capillary array-based sample screening
US09/894,956 2001-06-27
US09/894,956 US20020015997A1 (en) 1997-06-16 2001-06-27 Capillary array-based sample screening
US30910101P 2001-07-31 2001-07-31
US60/309,101 2001-07-31

Publications (3)

Publication Number Publication Date
WO2002031203A2 WO2002031203A2 (en) 2002-04-18
WO2002031203A9 WO2002031203A9 (en) 2003-07-03
WO2002031203A3 true WO2002031203A3 (en) 2003-09-25

Family

ID=27540945

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/031806 WO2002031203A2 (en) 2000-10-10 2001-10-10 High throughput or capillary-based screening for a bioactivity or biomolecule

Country Status (7)

Country Link
EP (1) EP1364052A2 (en)
JP (1) JP2005501217A (en)
AU (1) AU1164202A (en)
CA (1) CA2393374A1 (en)
IL (1) IL150020A0 (en)
MX (1) MXPA02005717A (en)
WO (1) WO2002031203A2 (en)

Families Citing this family (101)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10533998B2 (en) 2008-07-18 2020-01-14 Bio-Rad Laboratories, Inc. Enzyme quantification
GB0307428D0 (en) 2003-03-31 2003-05-07 Medical Res Council Compartmentalised combinatorial chemistry
GB0307403D0 (en) 2003-03-31 2003-05-07 Medical Res Council Selection by compartmentalised screening
US20060078893A1 (en) 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
EP2404931A1 (en) 2003-07-02 2012-01-11 Verenium Corporation Glucanases, nucleic acids encoding them and methods for making and using them
CA2535526C (en) 2003-08-11 2015-09-29 Diversa Corporation Laccases, nucleic acids encoding them and methods for making and using them
EP1681981A4 (en) * 2003-10-10 2008-04-23 Biophysical Corp Generation of biochemical images and methods of use
US20050221339A1 (en) 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
US20080070291A1 (en) 2004-06-16 2008-03-20 David Lam Compositions and Methods for Enzymatic Decolorization of Chlorophyll
AU2005264938B2 (en) 2004-06-16 2011-11-24 Verenium Corporation Compositions and methods for enzymatic decolorization of chlorophyll
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
WO2006099207A2 (en) 2005-03-10 2006-09-21 Diversa Corporation Lyase enzymes, nucleic acids encoding them and methods for making and using them
NZ594810A (en) 2005-03-15 2012-12-21 Verenium Corp Cellulases, nucleic acids encoding them and methods for making and using them
WO2007081386A2 (en) 2006-01-11 2007-07-19 Raindance Technologies, Inc. Microfluidic devices and methods of use
DK2450439T3 (en) 2006-02-10 2014-02-10 Verenium Corp Cellulose-degrading enzymes, nucleic acids encoding them, and methods of preparing and utilizing them
EP2548955A1 (en) 2006-02-14 2013-01-23 Verenium Corporation Xylanases, nucleic acids encoding them and methods for making and using them
EP2112227B1 (en) 2006-03-07 2013-05-15 Cargill, Incorporated Aldolases, nucleic acids encoding them and methods for making and using them
KR101588539B1 (en) 2006-03-07 2016-01-25 바스프 엔자임스 엘엘씨 Aldolases, nucleic acids encoding them and methods for making and using them
US20080014589A1 (en) 2006-05-11 2008-01-17 Link Darren R Microfluidic devices and methods of use thereof
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
BRPI0714876B1 (en) 2006-08-04 2022-04-19 Verenium Corporation Isolated, synthetic or recombinant nucleic acid, expression cassette, cloning vector or vehicle, transformed bacterial, fungal or yeast cell, isolated, synthetic or recombinant polypeptide, composition, as well as production methods and uses thereof
EP2077912B1 (en) 2006-08-07 2019-03-27 The President and Fellows of Harvard College Fluorocarbon emulsion stabilizing surfactants
CA2674721C (en) 2007-01-30 2018-04-03 Verenium Corporation Enzymes for the treatment of lignocellulosics, nucleic acids encoding them and methods for making and using them
US8772046B2 (en) 2007-02-06 2014-07-08 Brandeis University Manipulation of fluids and reactions in microfluidic systems
WO2008109176A2 (en) 2007-03-07 2008-09-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
US8592221B2 (en) 2007-04-19 2013-11-26 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
WO2009045627A2 (en) 2007-10-03 2009-04-09 Verenium Corporation Xylanases, nucleic acids encoding them and methods for making and using them
JP5738597B2 (en) 2007-12-21 2015-06-24 プレジデント アンド フェローズ オブ ハーバード カレッジ Systems and methods for nucleic acid sequencing
JP5563990B2 (en) 2008-01-03 2014-07-30 ヴェレニウム コーポレイション Transferases and oxidoreductases, nucleic acids encoding them and methods for making and using them
WO2010009365A1 (en) 2008-07-18 2010-01-21 Raindance Technologies, Inc. Droplet libraries
WO2010033200A2 (en) 2008-09-19 2010-03-25 President And Fellows Of Harvard College Creation of libraries of droplets and related species
EP2373812B1 (en) 2008-12-19 2016-11-09 President and Fellows of Harvard College Particle-assisted nucleic acid sequencing
US8528589B2 (en) 2009-03-23 2013-09-10 Raindance Technologies, Inc. Manipulation of microfluidic droplets
EP2486409A1 (en) 2009-10-09 2012-08-15 Universite De Strasbourg Labelled silica-based nanomaterial with enhanced properties and uses thereof
JP5791621B2 (en) 2009-10-27 2015-10-07 プレジデント アンド フェローズ オブ ハーバード カレッジ Droplet generation technology
US10837883B2 (en) 2009-12-23 2020-11-17 Bio-Rad Laboratories, Inc. Microfluidic systems and methods for reducing the exchange of molecules between droplets
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
WO2011100604A2 (en) 2010-02-12 2011-08-18 Raindance Technologies, Inc. Digital analyte analysis
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
AU2011221243B2 (en) * 2010-02-25 2016-06-02 Advanced Liquid Logic, Inc. Method of making nucleic acid libraries
EP2579040A4 (en) * 2010-05-25 2014-02-12 Nanoentek Inc Qualitative and quantitative analytical method for analyzing the activity type of an enzyme that is activated by proteolysis
EP3447155A1 (en) 2010-09-30 2019-02-27 Raindance Technologies, Inc. Sandwich assays in droplets
WO2012109600A2 (en) 2011-02-11 2012-08-16 Raindance Technologies, Inc. Methods for forming mixed droplets
WO2012112804A1 (en) 2011-02-18 2012-08-23 Raindance Technoligies, Inc. Compositions and methods for molecular labeling
WO2012149312A1 (en) * 2011-04-28 2012-11-01 The Regents Of The University Of California Time-gated fluorescence imaging with si-containing particles
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
DK2831587T3 (en) 2012-03-27 2018-07-23 Ventana Med Syst Inc Signaling conjugates and methods of use
US9657290B2 (en) * 2012-07-03 2017-05-23 The Board Of Trustees Of The Leland Stanford Junior University Scalable bio-element analysis
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014028537A1 (en) 2012-08-14 2014-02-20 10X Technologies, Inc. Microcapsule compositions and methods
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA2894694C (en) 2012-12-14 2023-04-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP3862435A1 (en) 2013-02-08 2021-08-11 10X Genomics, Inc. Polynucleotide barcode generation
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
WO2015069634A1 (en) 2013-11-08 2015-05-14 President And Fellows Of Harvard College Microparticles, methods for their preparation and use
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
EP3090063B1 (en) 2013-12-31 2019-11-06 Bio-Rad Laboratories, Inc. Method for detection of latent retrovirus
AU2015243445B2 (en) 2014-04-10 2020-05-28 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
CN106795553B (en) 2014-06-26 2021-06-04 10X基因组学有限公司 Methods of analyzing nucleic acids from individual cells or cell populations
KR20170023979A (en) 2014-06-26 2017-03-06 10엑스 제노믹스, 인크. Processes and systems for nucleic acid sequence assembly
CA2964472A1 (en) 2014-10-29 2016-05-06 10X Genomics, Inc. Methods and compositions for targeted nucleic acid sequencing
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
EP3244992B1 (en) 2015-01-12 2023-03-08 10X Genomics, Inc. Processes for barcoding nucleic acids
JP2018508852A (en) 2015-01-13 2018-03-29 １０エックス ゲノミクス，インコーポレイテッド System and method for visualizing structural variation and phase information
MX2017010142A (en) 2015-02-09 2017-12-11 10X Genomics Inc Systems and methods for determining structural variation and phasing using variant call data.
US10370653B2 (en) 2015-02-22 2019-08-06 The Board Of Trustees Of The Leland Stanford Junior University Micro-screening apparatus, process, and products
EP3262407B1 (en) 2015-02-24 2023-08-30 10X Genomics, Inc. Partition processing methods and systems
BR112017018054A2 (en) 2015-02-24 2018-07-24 10X Genomics Inc Methods for Covering Targeted Nucleic Acid Sequences
US10647981B1 (en) 2015-09-08 2020-05-12 Bio-Rad Laboratories, Inc. Nucleic acid library generation methods and compositions
WO2017066231A1 (en) 2015-10-13 2017-04-20 President And Fellows Of Harvard College Systems and methods for making and using gel microspheres
SG10202108763UA (en) 2015-12-04 2021-09-29 10X Genomics Inc Methods and compositions for nucleic acid analysis
SG11201806757XA (en) 2016-02-11 2018-09-27 10X Genomics Inc Systems, methods, and media for de novo assembly of whole genome sequence data
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
WO2018089953A1 (en) 2016-11-14 2018-05-17 Orca Biosystems, Inc. Methods and apparatuses for sorting target particles
US11085039B2 (en) 2016-12-12 2021-08-10 xCella Biosciences, Inc. Methods and systems for screening using microcapillary arrays
CA3046827A1 (en) 2016-12-12 2018-06-21 xCella Biosciences, Inc. Methods and systems for screening using microcapillary arrays
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
AU2017388058B2 (en) 2016-12-30 2023-02-02 xCella Biosciences, Inc. Multi-stage sample recovery system
WO2018140966A1 (en) 2017-01-30 2018-08-02 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
WO2018213774A1 (en) 2017-05-19 2018-11-22 10X Genomics, Inc. Systems and methods for analyzing datasets
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
SG11201901822QA (en) 2017-05-26 2019-03-28 10X Genomics Inc Single cell analysis of transposase accessible chromatin
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
SG11202009889VA (en) 2018-04-06 2020-11-27 10X Genomics Inc Systems and methods for quality control in single cell processing
CN109682786B (en) * 2019-01-16 2021-11-12 四川省畜牧科学研究院 Method for identifying haemonchus contortus eggs and application thereof
CN110047572A (en) * 2019-03-14 2019-07-23 周军河 A kind of intelligence warm spring system and application method
CN116675285B (en) * 2023-05-15 2023-11-10 苏州科技大学 Degradation method of perfluoro compound

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997031113A1 (en) * 1996-02-23 1997-08-28 Ariad Pharmaceuticals, Inc. Cell-based assay
US5777096A (en) * 1992-04-03 1998-07-07 The Perkin-Elmer Corporation Probe composition containing a binding domain and polymer chain and methods of use
WO1998058085A1 (en) * 1997-06-16 1998-12-23 Diversa Corporation High throughput screening for novel enzymes
US5853984A (en) * 1990-06-11 1998-12-29 Nexstar Pharmaceuticals, Inc. Use of nucleic acid ligands in flow cytometry
WO1999010539A1 (en) * 1997-08-26 1999-03-04 Diversa Corporation Screening for novel bioactivities
US5958672A (en) * 1995-07-18 1999-09-28 Diversa Corporation Protein activity screening of clones having DNA from uncultivated microorganisms
WO2000008212A1 (en) * 1998-08-07 2000-02-17 Cellay, Llc Gel microdrops in genetic analysis

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5853984A (en) * 1990-06-11 1998-12-29 Nexstar Pharmaceuticals, Inc. Use of nucleic acid ligands in flow cytometry
US5777096A (en) * 1992-04-03 1998-07-07 The Perkin-Elmer Corporation Probe composition containing a binding domain and polymer chain and methods of use
US5958672A (en) * 1995-07-18 1999-09-28 Diversa Corporation Protein activity screening of clones having DNA from uncultivated microorganisms
WO1997031113A1 (en) * 1996-02-23 1997-08-28 Ariad Pharmaceuticals, Inc. Cell-based assay
WO1998058085A1 (en) * 1997-06-16 1998-12-23 Diversa Corporation High throughput screening for novel enzymes
WO1999010539A1 (en) * 1997-08-26 1999-03-04 Diversa Corporation Screening for novel bioactivities
WO2000008212A1 (en) * 1998-08-07 2000-02-17 Cellay, Llc Gel microdrops in genetic analysis

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
WEAVER J C ET AL: "RAPID CLONAL GROWTH MEASUREMENTS AT THE SINGLE-CELL LEVEL: GEL MICRODROPLETS AND FLOW CYTOMETRY", BIO/TECHNOLOGY, NATURE PUBLISHING CO. NEW YORK, US, vol. 9, no. 9, 1 September 1991 (1991-09-01), pages 873 - 877, XP000371707, ISSN: 0733-222X *
WITTRUP K D ET AL: "Microencapsulation selection for isolation of yeast mutants with increased secretion of Aspergillus awamori glucoamylase", BIOTECHNOLOGY AND BIOENGINEERING. INCLUDING: SYMPOSIUM BIOTECHNOLOGY IN ENERGY PRODUCTION AND CONSERVATION, JOHN WILEY & SONS. NEW YORK, US, vol. 42, no. 3, 1993, pages 351 - 356, XP002151519, ISSN: 0006-3592 *

Also Published As

Publication number Publication date
WO2002031203A9 (en) 2003-07-03
MXPA02005717A (en) 2003-10-14
IL150020A0 (en) 2002-12-01
AU1164202A (en) 2002-04-22
WO2002031203A2 (en) 2002-04-18
EP1364052A2 (en) 2003-11-26
CA2393374A1 (en) 2002-04-18
JP2005501217A (en) 2005-01-13

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