WO2002042421A3 - Cell culture apparatus and methods of use - Google Patents

Cell culture apparatus and methods of use Download PDF

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Publication number
WO2002042421A3
WO2002042421A3 PCT/US2001/044128 US0144128W WO0242421A3 WO 2002042421 A3 WO2002042421 A3 WO 2002042421A3 US 0144128 W US0144128 W US 0144128W WO 0242421 A3 WO0242421 A3 WO 0242421A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell culture
culture apparatus
methods
frame
culture chamber
Prior art date
Application number
PCT/US2001/044128
Other languages
French (fr)
Other versions
WO2002042421A2 (en
Inventor
Emilio Barbera-Guillem
M Bud Nelson
Original Assignee
Biocrystal Ltd
Emilio Barbera-Guillem
M Bud Nelson
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/724,251 external-priority patent/US6479252B1/en
Priority claimed from US09/975,889 external-priority patent/US6749750B2/en
Application filed by Biocrystal Ltd, Emilio Barbera-Guillem, M Bud Nelson filed Critical Biocrystal Ltd
Priority to AU2002230479A priority Critical patent/AU2002230479A1/en
Publication of WO2002042421A2 publication Critical patent/WO2002042421A2/en
Publication of WO2002042421A3 publication Critical patent/WO2002042421A3/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/02—Magnetic separation acting directly on the substance being separated
    • B03C1/28—Magnetic plugs and dipsticks
    • B03C1/288—Magnetic plugs and dipsticks disposed at the outer circumference of a recipient
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/10—Petri dish
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/22—Transparent or translucent parts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/24—Gas permeable parts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/58—Reaction vessels connected in series or in parallel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/02—Membranes; Filters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/04—Seals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C2201/00—Details of magnetic or electrostatic separation
    • B03C2201/26—Details of magnetic or electrostatic separation for use in medical applications

Abstract

Provided is a cell culture apparatus (12) for culturing cells, and optionally, for performing magnetic separation of cells desired to be cultured. The cell culture apparatus (12) preferably comprises a frame (18); two membranes (31, 32), preferably each being gas permeable, which are securedly sealed in a leak-proof sealing to a frame (18) in forming a culture chamber there-between (40); and at least one resealable aperture (23) to allow substances to be introduced into, or withdrawn from, the culture chamber (40).
PCT/US2001/044128 2000-11-21 2001-11-21 Cell culture apparatus and methods of use WO2002042421A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002230479A AU2002230479A1 (en) 2000-11-21 2001-11-21 Cell culture apparatus and methods of use

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US71765100A 2000-11-21 2000-11-21
US72300800A 2000-11-27 2000-11-27
US09/723,008 2000-11-27
US09/724,251 US6479252B1 (en) 1999-03-23 2000-11-28 Cell culture apparatus and methods of use
US09/724,251 2000-11-28
US09/975,889 2001-10-11
US09/975,889 US6749750B2 (en) 2000-11-27 2001-10-11 Magnetic sheet assembly for magnetic separation
US09/717,651 2001-11-21

Publications (2)

Publication Number Publication Date
WO2002042421A2 WO2002042421A2 (en) 2002-05-30
WO2002042421A3 true WO2002042421A3 (en) 2003-01-23

Family

ID=27505540

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/044128 WO2002042421A2 (en) 2000-11-21 2001-11-21 Cell culture apparatus and methods of use

Country Status (2)

Country Link
AU (1) AU2002230479A1 (en)
WO (1) WO2002042421A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7514256B2 (en) 2005-02-11 2009-04-07 Emilio Barbera-Guillem Bioreactor for selectively controlling the molecular diffusion between fluids
GB2456875A (en) * 2008-01-31 2009-08-05 Eclipse Magnetics Ltd A seal for a magentic separator
EP2261325A1 (en) * 2009-06-09 2010-12-15 Helmholtz-Zentrum für Infektionsforschung Process for cell cultivation and cryopreservation
JP6083027B2 (en) * 2011-03-29 2017-02-22 ジクセル インク．Ｚｙｘｅｌｌ Ｉｎｃ． Multifunctional bioreactor system and method for sorting and culturing cells
GB201622341D0 (en) * 2016-12-28 2017-02-08 Auckland Uniservices Ltd Method
CN110438006B (en) * 2019-09-20 2024-03-01 桂林医学院 Wall-attached single-cell double-layer culture device

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0148161A2 (en) * 1984-01-03 1985-07-10 E.I. Du Pont De Nemours And Company Cell culture chamber
US5672505A (en) * 1993-09-27 1997-09-30 Becton, Dickinson And Company Insert for a issue culture vessel
US5935847A (en) * 1994-10-28 1999-08-10 Baxter International Inc. Multilayer gas-permeable container for the culture of adherent and non-adherent cells
US5939314A (en) * 1989-10-31 1999-08-17 The United States Of America As Represented The Administrator Of The National Aeronautics And Space Administration Distributed pore chemistry in porous organic polymers in tissue culture flasks

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0148161A2 (en) * 1984-01-03 1985-07-10 E.I. Du Pont De Nemours And Company Cell culture chamber
US5939314A (en) * 1989-10-31 1999-08-17 The United States Of America As Represented The Administrator Of The National Aeronautics And Space Administration Distributed pore chemistry in porous organic polymers in tissue culture flasks
US5672505A (en) * 1993-09-27 1997-09-30 Becton, Dickinson And Company Insert for a issue culture vessel
US5935847A (en) * 1994-10-28 1999-08-10 Baxter International Inc. Multilayer gas-permeable container for the culture of adherent and non-adherent cells

Also Published As

Publication number Publication date
AU2002230479A1 (en) 2002-06-03
WO2002042421A2 (en) 2002-05-30

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