WO2002061485A3 - High-resolution optical microscope - Google Patents

High-resolution optical microscope Download PDF

Info

Publication number
WO2002061485A3
WO2002061485A3 PCT/US2001/046397 US0146397W WO02061485A3 WO 2002061485 A3 WO2002061485 A3 WO 2002061485A3 US 0146397 W US0146397 W US 0146397W WO 02061485 A3 WO02061485 A3 WO 02061485A3
Authority
WO
WIPO (PCT)
Prior art keywords
light
combined
optical microscope
intensity
resolution optical
Prior art date
Application number
PCT/US2001/046397
Other languages
French (fr)
Other versions
WO2002061485A2 (en
Inventor
Vitaly J Vodyanoy
William Charles Neely
Original Assignee
Univ Auburn
Vitaly J Vodyanoy
William Charles Neely
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Auburn, Vitaly J Vodyanoy, William Charles Neely filed Critical Univ Auburn
Priority to AU2002248163A priority Critical patent/AU2002248163A1/en
Priority to EP01997041A priority patent/EP1384103A2/en
Publication of WO2002061485A2 publication Critical patent/WO2002061485A2/en
Publication of WO2002061485A3 publication Critical patent/WO2002061485A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/65—Raman scattering
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/06—Means for illuminating specimens
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/18—Arrangements with more than one light path, e.g. for comparing two specimens
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements

Abstract

A direct-view optical microscope system is provided which uses high-energy light from a phenomenon known as non-resonant Raman scattering to illuminate a living biological specimen. One embodiment of the system combines two discrete light sources to form a combined incident light source for the microscope. The system includes a method and apparatus for modulating the intensity of the scattered light when two light waves are combined to produce the incident light. By varying the frequency of the two source light waves, the intensity of the combined Raman-scattered light can be modulated to achieve finer resolution.
PCT/US2001/046397 2000-12-01 2001-12-03 High-resolution optical microscope WO2002061485A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2002248163A AU2002248163A1 (en) 2000-12-01 2001-12-03 High-resolution optical microscope
EP01997041A EP1384103A2 (en) 2000-12-01 2001-12-03 High-resolution optical microscope

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US25080000P 2000-12-01 2000-12-01
US60/250,800 2000-12-01

Publications (2)

Publication Number Publication Date
WO2002061485A2 WO2002061485A2 (en) 2002-08-08
WO2002061485A3 true WO2002061485A3 (en) 2003-11-27

Family

ID=22949203

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/046397 WO2002061485A2 (en) 2000-12-01 2001-12-03 High-resolution optical microscope

Country Status (4)

Country Link
US (3) US6690509B2 (en)
EP (1) EP1384103A2 (en)
AU (1) AU2002248163A1 (en)
WO (1) WO2002061485A2 (en)

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Also Published As

Publication number Publication date
US6865013B2 (en) 2005-03-08
US20050168810A1 (en) 2005-08-04
EP1384103A2 (en) 2004-01-28
US20040090669A1 (en) 2004-05-13
WO2002061485A2 (en) 2002-08-08
AU2002248163A1 (en) 2002-08-12
US6690509B2 (en) 2004-02-10
US20020135871A1 (en) 2002-09-26
US7106503B2 (en) 2006-09-12

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