WO2002071929A3 - Methods of decreasing non-specific binding in dual bead assays and system apparatus for detecting medical targets - Google Patents

Methods of decreasing non-specific binding in dual bead assays and system apparatus for detecting medical targets Download PDF

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Publication number
WO2002071929A3
WO2002071929A3 PCT/US2002/007955 US0207955W WO02071929A3 WO 2002071929 A3 WO2002071929 A3 WO 2002071929A3 US 0207955 W US0207955 W US 0207955W WO 02071929 A3 WO02071929 A3 WO 02071929A3
Authority
WO
WIPO (PCT)
Prior art keywords
beads
methods
specific binding
section
reporter
Prior art date
Application number
PCT/US2002/007955
Other languages
French (fr)
Other versions
WO2002071929A2 (en
Inventor
Brigitte Chau Phan
Jorma Antero Virtanen
Amethyst Hoang Lam
Ka-Yeun Yeung
James Howard Coombs
Original Assignee
Burnstein Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/997,741 external-priority patent/US20030003464A1/en
Priority claimed from US10/038,297 external-priority patent/US20030077598A1/en
Application filed by Burnstein Technologies Inc filed Critical Burnstein Technologies Inc
Priority to AU2002258528A priority Critical patent/AU2002258528A1/en
Publication of WO2002071929A2 publication Critical patent/WO2002071929A2/en
Publication of WO2002071929A3 publication Critical patent/WO2002071929A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/72—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating magnetic variables
    • G01N27/74—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating magnetic variables of fluids
    • G01N27/745—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating magnetic variables of fluids for detecting magnetic beads used in biochemical assays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/005—Pretreatment specially adapted for magnetic separation
    • B03C1/01—Pretreatment specially adapted for magnetic separation by addition of magnetic adjuvants
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/02—Magnetic separation acting directly on the substance being separated
    • B03C1/28—Magnetic plugs and dipsticks
    • B03C1/288—Magnetic plugs and dipsticks disposed at the outer circumference of a recipient
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C2201/00—Details of magnetic or electrostatic separation
    • B03C2201/18—Magnetic separation whereby the particles are suspended in a liquid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C2201/00—Details of magnetic or electrostatic separation
    • B03C2201/26—Details of magnetic or electrostatic separation for use in medical applications

Abstract

Methods for decreasing non-specific bindings of beads in dual assays and related optical bio-discs (110) and disc drive system. The optical bio-disc comprise of a magnetic region (242) and magnetic capture region (243). In figure 37, section A refer to individual magnetic beads, section B refers to after binding of reporter beads, and section C refers to dual bead complexes. Methods include identifying whether a target agent is present in a biological sample and mixing capture beads each having at least one transport probe affixed thereto, reporter beads having at least one signal probe thereto, and a biological sample. The methods may further include selectively breaking up non-specific binding between capture beads and reporter beads employing a digestion agent. Also is a method for selectively breaking up non-specific binding between captures beads and reporter beads using wash buffer containing a chemical agent. The methods are applied to detecting medical targets.
PCT/US2002/007955 2001-03-14 2002-03-14 Methods of decreasing non-specific binding in dual bead assays and system apparatus for detecting medical targets WO2002071929A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002258528A AU2002258528A1 (en) 2001-03-14 2002-03-14 Methods of decreasing non-specific binding in dual bead assays and system apparatus for detecting medical targets

Applications Claiming Priority (16)

Application Number Priority Date Filing Date Title
US27564301P 2001-03-14 2001-03-14
US60/275,643 2001-03-14
US27811001P 2001-03-23 2001-03-23
US27810601P 2001-03-23 2001-03-23
US60/278,110 2001-03-23
US60/278,106 2001-03-23
US27869701P 2001-03-26 2001-03-26
US60/278,697 2001-03-26
US31490601P 2001-08-24 2001-08-24
US60/314,906 2001-08-24
US09/997,741 US20030003464A1 (en) 2000-11-27 2001-11-27 Dual bead assays including optical biodiscs and methods relating thereto
US09/997,741 2001-11-27
US10/038,297 2002-01-04
US10/038,297 US20030077598A1 (en) 2001-01-04 2002-01-04 Dual bead assays including covalent linkages for improved specificity and related optical analysis discs
US35227002P 2002-01-30 2002-01-30
US60/352,270 2002-01-30

Publications (2)

Publication Number Publication Date
WO2002071929A2 WO2002071929A2 (en) 2002-09-19
WO2002071929A3 true WO2002071929A3 (en) 2003-03-20

Family

ID=27574253

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/007955 WO2002071929A2 (en) 2001-03-14 2002-03-14 Methods of decreasing non-specific binding in dual bead assays and system apparatus for detecting medical targets

Country Status (2)

Country Link
AU (1) AU2002258528A1 (en)
WO (1) WO2002071929A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9103794B2 (en) 2001-08-27 2015-08-11 Platypus Technologies Llc Substrates, devices, and methods for quantitative liquid crystal assays

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002068697A2 (en) * 2001-02-28 2002-09-06 Burstein Technologies, Inc. Methods for decreasing non-specific binding of beads in dual bead assays including related optical biodiscs and disc drive systems
WO2005047863A2 (en) 2003-11-10 2005-05-26 Platypus Technologies, Llc Substrates, devices, and methods for cellular assays
JP4950663B2 (en) 2003-07-25 2012-06-13 プラティパス テクノロジーズ エルエルシー Liquid crystal based analyte detection
US7531366B2 (en) 2004-07-23 2009-05-12 Platypus Technologies, Llc Bead based assays using a liquid crystal reporter
WO2007025129A2 (en) 2005-08-25 2007-03-01 Platypus Technologies, Llc. Compositions and liquid crystals
US7723120B2 (en) 2005-10-26 2010-05-25 General Electric Company Optical sensor array system and method for parallel processing of chemical and biochemical information
US8133741B2 (en) 2005-10-26 2012-03-13 General Electric Company Methods and systems for delivery of fluidic samples to sensor arrays
ES2338165T3 (en) * 2005-10-26 2010-05-04 General Electric Company PROCEDURES AND SYSTEMS TO SUPPLY FLUID SAMPLES TO SENSOR SERIES.
EP1971691A2 (en) * 2006-01-09 2008-09-24 Platypus Technologies, LLC Bead based assays using a liquid crystal reporter
US7842499B2 (en) 2006-08-07 2010-11-30 Platypus Technologies, Llc Substrates, devices, and methods for cellular assays
US7883898B2 (en) 2007-05-07 2011-02-08 General Electric Company Method and apparatus for measuring pH of low alkalinity solutions
EP2193365A4 (en) 2007-08-20 2015-05-13 Platypus Technologies Llc Improved devices for cell assays
US8178355B2 (en) 2008-09-15 2012-05-15 Platypus Technologies, Llc. Detection of vapor phase compounds by changes in physical properties of a liquid crystal
EP3693739A1 (en) 2019-02-06 2020-08-12 Stefan Schreier Method and device for isolating desired cells from a sample of nonmagnetic biological material

Citations (3)

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US5755942A (en) * 1994-11-10 1998-05-26 David Sarnoff Research Center, Inc. Partitioned microelectronic device array
US5922617A (en) * 1997-11-12 1999-07-13 Functional Genetics, Inc. Rapid screening assay methods and devices
US5981180A (en) * 1995-10-11 1999-11-09 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and methods

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5755942A (en) * 1994-11-10 1998-05-26 David Sarnoff Research Center, Inc. Partitioned microelectronic device array
US5981180A (en) * 1995-10-11 1999-11-09 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and methods
US5922617A (en) * 1997-11-12 1999-07-13 Functional Genetics, Inc. Rapid screening assay methods and devices

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9103794B2 (en) 2001-08-27 2015-08-11 Platypus Technologies Llc Substrates, devices, and methods for quantitative liquid crystal assays

Also Published As

Publication number Publication date
AU2002258528A1 (en) 2002-09-24
WO2002071929A2 (en) 2002-09-19

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