WO2002072773A3 - Methods and compositions for amplification of rna sequences - Google Patents
Methods and compositions for amplification of rna sequences Download PDFInfo
- Publication number
- WO2002072773A3 WO2002072773A3 PCT/US2002/007377 US0207377W WO02072773A3 WO 2002072773 A3 WO2002072773 A3 WO 2002072773A3 US 0207377 W US0207377 W US 0207377W WO 02072773 A3 WO02072773 A3 WO 02072773A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- methods
- amplification
- rna
- compositions
- rna sequences
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Plant Pathology (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP02731119.0A EP1390537B1 (en) | 2001-03-09 | 2002-03-11 | Methods and compositions for amplification of rna sequences |
JP2002571829A JP2005508135A (en) | 2001-03-09 | 2002-03-11 | Methods and compositions for amplification of RNA sequences |
CA002439074A CA2439074A1 (en) | 2001-03-09 | 2002-03-11 | Methods and compositions for amplification of rna sequences |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US27423601P | 2001-03-09 | 2001-03-09 | |
US60/274,236 | 2001-03-09 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2002072773A2 WO2002072773A2 (en) | 2002-09-19 |
WO2002072773A3 true WO2002072773A3 (en) | 2003-12-18 |
Family
ID=23047369
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2002/007377 WO2002072773A2 (en) | 2001-03-09 | 2002-03-11 | Methods and compositions for amplification of rna sequences |
Country Status (5)
Country | Link |
---|---|
US (2) | US7094536B2 (en) |
EP (1) | EP1390537B1 (en) |
JP (1) | JP2005508135A (en) |
CA (1) | CA2439074A1 (en) |
WO (1) | WO2002072773A2 (en) |
Cited By (2)
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---|---|---|---|---|
US8143001B2 (en) | 2003-12-29 | 2012-03-27 | Nugen Technologies, Inc. | Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids |
US9206418B2 (en) | 2011-10-19 | 2015-12-08 | Nugen Technologies, Inc. | Compositions and methods for directional nucleic acid amplification and sequencing |
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US6692918B2 (en) * | 1999-09-13 | 2004-02-17 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
DE60141087D1 (en) | 2000-06-26 | 2010-03-04 | Nugen Technologies Inc | METHODS AND COMPOSITIONS FOR TRANSCRIPTION-REPRODUCTIVE MACHINING OF NUCLEIC ACIDS |
US7846733B2 (en) | 2000-06-26 | 2010-12-07 | Nugen Technologies, Inc. | Methods and compositions for transcription-based nucleic acid amplification |
US6858413B2 (en) | 2000-12-13 | 2005-02-22 | Nugen Technologies, Inc. | Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof |
BR0205268A (en) | 2001-03-09 | 2004-11-30 | Nugen Technologies Inc | Processes and compositions for mRNA sequence mplification |
EP1390537B1 (en) * | 2001-03-09 | 2013-11-13 | Nugen Technologies, Inc. | Methods and compositions for amplification of rna sequences |
US7348142B2 (en) * | 2002-03-29 | 2008-03-25 | Veridex, Lcc | Cancer diagnostic panel |
EP1573056A4 (en) * | 2002-05-17 | 2007-11-28 | Nugen Technologies Inc | Methods for fragmentation, labeling and immobilization of nucleic acids |
DE10315074A1 (en) * | 2003-04-02 | 2004-10-14 | Clondiag Chip Technologies Gmbh | Device for the duplication and detection of nucleic acids |
CA2521084A1 (en) | 2003-04-14 | 2004-10-28 | Nugen Technologies, Inc. | Global amplification using a randomly primed composite primer |
US7524672B2 (en) * | 2004-09-22 | 2009-04-28 | Sandia Corporation | Microfluidic microarray systems and methods thereof |
US7592139B2 (en) | 2004-09-24 | 2009-09-22 | Sandia National Laboratories | High temperature flow-through device for rapid solubilization and analysis |
FR2876116B1 (en) * | 2004-10-01 | 2007-04-06 | Biomerieux Sa | METHOD FOR GENERATING TRANSCRIPTS |
WO2007030759A2 (en) | 2005-09-07 | 2007-03-15 | Nugen Technologies, Inc. | Improved nucleic acid amplification procedure |
US20090275486A1 (en) * | 2006-05-16 | 2009-11-05 | Nurith Kurn | Nucleic acid separation and purification method based on reversible charge interactions |
US7833716B2 (en) | 2006-06-06 | 2010-11-16 | Gen-Probe Incorporated | Tagged oligonucleotides and their use in nucleic acid amplification methods |
US8088580B2 (en) * | 2006-06-07 | 2012-01-03 | Sumitomo Bakelite Company, Ltd. | RNA detection method |
US20100022403A1 (en) * | 2006-06-30 | 2010-01-28 | Nurith Kurn | Methods for fragmentation and labeling of nucleic acids |
US20090047673A1 (en) * | 2006-08-22 | 2009-02-19 | Cary Robert B | Miniaturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids |
US8980561B1 (en) | 2006-08-22 | 2015-03-17 | Los Alamos National Security, Llc. | Nucleic acid detection system and method for detecting influenza |
US9388457B2 (en) | 2007-09-14 | 2016-07-12 | Affymetrix, Inc. | Locus specific amplification using array probes |
US8716190B2 (en) | 2007-09-14 | 2014-05-06 | Affymetrix, Inc. | Amplification and analysis of selected targets on solid supports |
US20090203531A1 (en) * | 2008-02-12 | 2009-08-13 | Nurith Kurn | Method for Archiving and Clonal Expansion |
WO2009117698A2 (en) | 2008-03-21 | 2009-09-24 | Nugen Technologies, Inc. | Methods of rna amplification in the presence of dna |
EP2279403B1 (en) | 2008-05-05 | 2016-03-16 | Los Alamos National Security, LLC | Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control |
WO2011032053A1 (en) * | 2009-09-11 | 2011-03-17 | Nugen Technologies, Inc. | Compositions and methods for whole transcriptome analysis |
US8974651B2 (en) | 2010-04-17 | 2015-03-10 | C.C. Imex | Illuminator for visualization of fluorophores |
DK2699700T3 (en) | 2011-04-20 | 2016-08-01 | Mesa Biotech Inc | Integrated device for nukleinsyreregistrering and identification |
CN105861487B (en) | 2012-01-26 | 2020-05-05 | 纽亘技术公司 | Compositions and methods for targeted nucleic acid sequence enrichment and efficient library generation |
CN104619894B (en) | 2012-06-18 | 2017-06-06 | 纽亘技术公司 | For the composition and method of the Solid phase of unexpected nucleotide sequence |
US20150011396A1 (en) | 2012-07-09 | 2015-01-08 | Benjamin G. Schroeder | Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing |
EP2971130A4 (en) | 2013-03-15 | 2016-10-05 | Nugen Technologies Inc | Sequential sequencing |
EP3461913B1 (en) | 2013-08-09 | 2020-06-24 | Luminex Corporation | Probes for improved melt discrimination and multiplexing in nucleic acid assays |
WO2015073711A1 (en) | 2013-11-13 | 2015-05-21 | Nugen Technologies, Inc. | Compositions and methods for identification of a duplicate sequencing read |
WO2015131107A1 (en) | 2014-02-28 | 2015-09-03 | Nugen Technologies, Inc. | Reduced representation bisulfite sequencing with diversity adaptors |
US9835587B2 (en) | 2014-04-01 | 2017-12-05 | C.C. Imex | Electrophoresis running tank assembly |
CN107075581B (en) | 2014-08-06 | 2022-03-18 | 纽亘技术公司 | Digital measurement by targeted sequencing |
US10738349B2 (en) | 2015-10-23 | 2020-08-11 | Emory University | Polynucleotide based movement, kits and methods related thereto |
US11193153B2 (en) | 2017-03-20 | 2021-12-07 | President And Fellows Of Harvard College | Programmable nucleic acid synthesis cascade |
US11099202B2 (en) | 2017-10-20 | 2021-08-24 | Tecan Genomics, Inc. | Reagent delivery system |
Citations (1)
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US6251639B1 (en) * | 1999-09-13 | 2001-06-26 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences, using a RNA-DNA composite primer |
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-
2002
- 2002-03-11 EP EP02731119.0A patent/EP1390537B1/en not_active Expired - Lifetime
- 2002-03-11 JP JP2002571829A patent/JP2005508135A/en active Pending
- 2002-03-11 US US10/096,221 patent/US7094536B2/en not_active Expired - Fee Related
- 2002-03-11 WO PCT/US2002/007377 patent/WO2002072773A2/en active Search and Examination
- 2002-03-11 CA CA002439074A patent/CA2439074A1/en not_active Abandoned
-
2005
- 2005-06-08 US US11/148,593 patent/US7351557B2/en not_active Expired - Fee Related
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6251639B1 (en) * | 1999-09-13 | 2001-06-26 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences, using a RNA-DNA composite primer |
Non-Patent Citations (1)
Title |
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See also references of EP1390537A4 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8143001B2 (en) | 2003-12-29 | 2012-03-27 | Nugen Technologies, Inc. | Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids |
US9206418B2 (en) | 2011-10-19 | 2015-12-08 | Nugen Technologies, Inc. | Compositions and methods for directional nucleic acid amplification and sequencing |
Also Published As
Publication number | Publication date |
---|---|
US20020164628A1 (en) | 2002-11-07 |
US7351557B2 (en) | 2008-04-01 |
US20060014182A1 (en) | 2006-01-19 |
CA2439074A1 (en) | 2002-09-19 |
EP1390537B1 (en) | 2013-11-13 |
EP1390537A2 (en) | 2004-02-25 |
JP2005508135A (en) | 2005-03-31 |
EP1390537A4 (en) | 2006-04-26 |
WO2002072773A2 (en) | 2002-09-19 |
US7094536B2 (en) | 2006-08-22 |
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