WO2002072773A3 - Methods and compositions for amplification of rna sequences - Google Patents
Methods and compositions for amplification of rna sequences Download PDFInfo
- Publication number
- WO2002072773A3 WO2002072773A3 PCT/US2002/007377 US0207377W WO02072773A3 WO 2002072773 A3 WO2002072773 A3 WO 2002072773A3 US 0207377 W US0207377 W US 0207377W WO 02072773 A3 WO02072773 A3 WO 02072773A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- methods
- amplification
- rna
- compositions
- rna sequences
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
Abstract
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2002571829A JP2005508135A (en) | 2001-03-09 | 2002-03-11 | Methods and compositions for amplification of RNA sequences |
CA002439074A CA2439074A1 (en) | 2001-03-09 | 2002-03-11 | Methods and compositions for amplification of rna sequences |
EP02731119.0A EP1390537B1 (en) | 2001-03-09 | 2002-03-11 | Methods and compositions for amplification of rna sequences |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US27423601P | 2001-03-09 | 2001-03-09 | |
US60/274,236 | 2001-03-09 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2002072773A2 WO2002072773A2 (en) | 2002-09-19 |
WO2002072773A3 true WO2002072773A3 (en) | 2003-12-18 |
Family
ID=23047369
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2002/007377 WO2002072773A2 (en) | 2001-03-09 | 2002-03-11 | Methods and compositions for amplification of rna sequences |
Country Status (5)
Country | Link |
---|---|
US (2) | US7094536B2 (en) |
EP (1) | EP1390537B1 (en) |
JP (1) | JP2005508135A (en) |
CA (1) | CA2439074A1 (en) |
WO (1) | WO2002072773A2 (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8143001B2 (en) | 2003-12-29 | 2012-03-27 | Nugen Technologies, Inc. | Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids |
US9206418B2 (en) | 2011-10-19 | 2015-12-08 | Nugen Technologies, Inc. | Compositions and methods for directional nucleic acid amplification and sequencing |
Families Citing this family (40)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6692918B2 (en) * | 1999-09-13 | 2004-02-17 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
ATE455186T1 (en) * | 2000-06-26 | 2010-01-15 | Nugen Technologies Inc | METHODS AND COMPOSITIONS FOR TRANSCRIPTION-BASED DUPLICATION OF NUCLEIC ACIDS |
US7846733B2 (en) | 2000-06-26 | 2010-12-07 | Nugen Technologies, Inc. | Methods and compositions for transcription-based nucleic acid amplification |
US6858413B2 (en) | 2000-12-13 | 2005-02-22 | Nugen Technologies, Inc. | Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof |
ATE361996T1 (en) | 2001-03-09 | 2007-06-15 | Nugen Technologies Inc | METHODS AND COMPOSITIONS FOR DUPLICATION OF RNA SEQUENCES |
JP2005508135A (en) * | 2001-03-09 | 2005-03-31 | ニューゲン テクノロジーズ, インコーポレイテッド | Methods and compositions for amplification of RNA sequences |
US7348142B2 (en) * | 2002-03-29 | 2008-03-25 | Veridex, Lcc | Cancer diagnostic panel |
US20040005614A1 (en) * | 2002-05-17 | 2004-01-08 | Nurith Kurn | Methods for fragmentation, labeling and immobilization of nucleic acids |
DE10315074A1 (en) * | 2003-04-02 | 2004-10-14 | Clondiag Chip Technologies Gmbh | Device for the duplication and detection of nucleic acids |
AU2004230494A1 (en) * | 2003-04-14 | 2004-10-28 | Nugen Technologies, Inc. | Global amplification using a randomly primed composite primer |
US7524672B2 (en) * | 2004-09-22 | 2009-04-28 | Sandia Corporation | Microfluidic microarray systems and methods thereof |
US7592139B2 (en) | 2004-09-24 | 2009-09-22 | Sandia National Laboratories | High temperature flow-through device for rapid solubilization and analysis |
FR2876116B1 (en) * | 2004-10-01 | 2007-04-06 | Biomerieux Sa | METHOD FOR GENERATING TRANSCRIPTS |
CA2621267A1 (en) | 2005-09-07 | 2007-03-15 | Nugen Technologies, Inc. | Improved nucleic acid amplification procedure |
US20090275486A1 (en) * | 2006-05-16 | 2009-11-05 | Nurith Kurn | Nucleic acid separation and purification method based on reversible charge interactions |
US7833716B2 (en) | 2006-06-06 | 2010-11-16 | Gen-Probe Incorporated | Tagged oligonucleotides and their use in nucleic acid amplification methods |
WO2007141912A1 (en) * | 2006-06-07 | 2007-12-13 | Sumitomo Bakelite Co., Ltd. | Rna detection method |
EP2038429B1 (en) * | 2006-06-30 | 2013-08-21 | Nugen Technologies, Inc. | Methods for fragmentation and labeling of nucleic acids |
US8980561B1 (en) | 2006-08-22 | 2015-03-17 | Los Alamos National Security, Llc. | Nucleic acid detection system and method for detecting influenza |
WO2008105814A2 (en) | 2006-08-22 | 2008-09-04 | Los Alamos National Security, Llc | Miniturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids |
US9388457B2 (en) * | 2007-09-14 | 2016-07-12 | Affymetrix, Inc. | Locus specific amplification using array probes |
US8716190B2 (en) | 2007-09-14 | 2014-05-06 | Affymetrix, Inc. | Amplification and analysis of selected targets on solid supports |
US8034568B2 (en) | 2008-02-12 | 2011-10-11 | Nugen Technologies, Inc. | Isothermal nucleic acid amplification methods and compositions |
WO2009117698A2 (en) | 2008-03-21 | 2009-09-24 | Nugen Technologies, Inc. | Methods of rna amplification in the presence of dna |
CN102084238B (en) | 2008-05-05 | 2016-06-01 | 洛斯阿拉莫斯国家安全有限责任公司 | Prepared by the nucleic acid samples based on highly simplified lateral flow and the flowing of passive fluid controls |
CA2773887A1 (en) * | 2009-09-11 | 2011-03-17 | Nugen Technologies, Inc. | Compositions and methods for whole transcriptome analysis |
US8974651B2 (en) | 2010-04-17 | 2015-03-10 | C.C. Imex | Illuminator for visualization of fluorophores |
WO2012145725A2 (en) | 2011-04-20 | 2012-10-26 | Mesa Tech International, Inc. | Oscillating amplification reaction for nucleic acids |
SG10201504490QA (en) | 2012-01-26 | 2015-07-30 | Nugen Technologies Inc | Compositions And Methods For Targeted Nucleic Acid Sequence Enrichment And High Efficiency Library Generation |
SG11201408478QA (en) | 2012-06-18 | 2015-02-27 | Nugen Technologies Inc | Compositions and methods for negative selection of non-desired nucleic acid sequences |
US20150011396A1 (en) | 2012-07-09 | 2015-01-08 | Benjamin G. Schroeder | Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing |
WO2014144092A1 (en) | 2013-03-15 | 2014-09-18 | Nugen Technologies, Inc. | Sequential sequencing |
EP3030680B1 (en) * | 2013-08-09 | 2018-10-03 | Luminex Corporation | Melt discrimination and multiplexing in nucleic acid assays |
WO2015073711A1 (en) | 2013-11-13 | 2015-05-21 | Nugen Technologies, Inc. | Compositions and methods for identification of a duplicate sequencing read |
WO2015131107A1 (en) | 2014-02-28 | 2015-09-03 | Nugen Technologies, Inc. | Reduced representation bisulfite sequencing with diversity adaptors |
US9835587B2 (en) | 2014-04-01 | 2017-12-05 | C.C. Imex | Electrophoresis running tank assembly |
JP6803327B2 (en) | 2014-08-06 | 2020-12-23 | ニューゲン テクノロジーズ, インコーポレイテッド | Digital measurements from targeted sequencing |
US10738349B2 (en) | 2015-10-23 | 2020-08-11 | Emory University | Polynucleotide based movement, kits and methods related thereto |
WO2018175296A2 (en) | 2017-03-20 | 2018-09-27 | President And Fellows Of Harvard College | Programmable nucleic acid synthesis cascade |
US11099202B2 (en) | 2017-10-20 | 2021-08-24 | Tecan Genomics, Inc. | Reagent delivery system |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6251639B1 (en) * | 1999-09-13 | 2001-06-26 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences, using a RNA-DNA composite primer |
Family Cites Families (89)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4582788A (en) | 1982-01-22 | 1986-04-15 | Cetus Corporation | HLA typing method and cDNA probes used therein |
US4786600A (en) | 1984-05-25 | 1988-11-22 | The Trustees Of Columbia University In The City Of New York | Autocatalytic replication of recombinant RNA |
US4683194A (en) | 1984-05-29 | 1987-07-28 | Cetus Corporation | Method for detection of polymorphic restriction sites and nucleic acid sequences |
US4876187A (en) | 1985-12-05 | 1989-10-24 | Meiogenics, Inc. | Nucleic acid compositions with scissile linkage useful for detecting nucleic acid sequences |
US5011769A (en) | 1985-12-05 | 1991-04-30 | Meiogenics U.S. Limited Partnership | Methods for detecting nucleic acid sequences |
DE3634356A1 (en) | 1986-10-08 | 1988-04-21 | Epis Sa | MEDICINE CONTAINING ALPHA-HALOGENED DICARBONIC ACIDS |
US6090591A (en) | 1987-07-31 | 2000-07-18 | The Board Of Trustees Of The Leland Stanford Junior University | Selective amplification of target polynucleotide sequences |
WO1989001050A1 (en) * | 1987-07-31 | 1989-02-09 | The Board Of Trustees Of The Leland Stanford Junior University | Selective amplification of target polynucleotide sequences |
US6004745A (en) | 1987-09-21 | 1999-12-21 | Gen-Probe Incorporated | Hybridization protection assay |
US5403711A (en) | 1987-11-30 | 1995-04-04 | University Of Iowa Research Foundation | Nucleic acid hybridization and amplification method for detection of specific sequences in which a complementary labeled nucleic acid probe is cleaved |
JP2650159B2 (en) | 1988-02-24 | 1997-09-03 | アクゾ・ノベル・エヌ・ベー | Nucleic acid amplification method |
CA1340807C (en) | 1988-02-24 | 1999-11-02 | Lawrence T. Malek | Nucleic acid amplification process |
US5130238A (en) | 1988-06-24 | 1992-07-14 | Cangene Corporation | Enhanced nucleic acid amplification process |
US5185243A (en) | 1988-08-25 | 1993-02-09 | Syntex (U.S.A.) Inc. | Method for detection of specific nucleic acid sequences |
US5508178A (en) | 1989-01-19 | 1996-04-16 | Rose; Samuel | Nucleic acid amplification using single primer |
US5043272A (en) | 1989-04-27 | 1991-08-27 | Life Technologies, Incorporated | Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers |
US5143854A (en) | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
KR100242252B1 (en) | 1989-07-11 | 2000-03-02 | 다니엘 엘. 캐시앙 | Nucleic acid sequence amplification methods |
CA2020958C (en) * | 1989-07-11 | 2005-01-11 | Daniel L. Kacian | Nucleic acid sequence amplification methods |
US5766849A (en) | 1989-07-11 | 1998-06-16 | Gen-Probe Incorporated | Methods of amplifying nucleic acids using promoter-containing primer sequence |
US5545522A (en) | 1989-09-22 | 1996-08-13 | Van Gelder; Russell N. | Process for amplifying a target polynucleotide sequence using a single primer-promoter complex |
US6013431A (en) | 1990-02-16 | 2000-01-11 | Molecular Tool, Inc. | Method for determining specific nucleotide variations by primer extension in the presence of mixture of labeled nucleotides and terminators |
CA2037349C (en) | 1990-03-26 | 2008-06-17 | James G. Wetmur | Branch migration of nucleotides |
US5427911A (en) | 1990-05-01 | 1995-06-27 | Yale University | Coupled amplification and sequencing of DNA |
HU218095B (en) | 1990-05-01 | 2000-05-28 | Amgen Inc. | Process for reducing transitional contaminations in amplification processes |
US5194370A (en) | 1990-05-16 | 1993-03-16 | Life Technologies, Inc. | Promoter ligation activated transcription amplification of nucleic acid sequences |
US5595891A (en) | 1990-07-19 | 1997-01-21 | Behringwerke Ag | Method for producing a polynucleotide for use in single primer amplification |
US5846710A (en) | 1990-11-02 | 1998-12-08 | St. Louis University | Method for the detection of genetic diseases and gene sequence variations by single nucleotide primer extension |
US6004744A (en) | 1991-03-05 | 1999-12-21 | Molecular Tool, Inc. | Method for determining nucleotide identity through extension of immobilized primer |
US5888819A (en) | 1991-03-05 | 1999-03-30 | Molecular Tool, Inc. | Method for determining nucleotide identity through primer extension |
WO1992018521A1 (en) * | 1991-04-10 | 1992-10-29 | Life Technologies, Inc. | Method for amplifying and altering an rna sequence |
US5169766A (en) * | 1991-06-14 | 1992-12-08 | Life Technologies, Inc. | Amplification of nucleic acid molecules |
CA2102784A1 (en) | 1992-03-11 | 1993-09-12 | Peng Liang | Identifying, isolating and cloning messenger rnas |
WO1993022461A1 (en) | 1992-05-06 | 1993-11-11 | Gen-Probe Incorporated | Nucleic acid sequence amplification method, composition and kit |
US5710028A (en) | 1992-07-02 | 1998-01-20 | Eyal; Nurit | Method of quick screening and identification of specific DNA sequences by single nucleotide primer extension and kits therefor |
JPH07509365A (en) | 1992-07-31 | 1995-10-19 | デイド・ベーリング・マルブルク・ゲゼルシヤフト・ミツト・ベシユレンクテル・ハフツング | Method for introducing a specific sequence into the 3' end of polynucleotides |
ZA936016B (en) | 1992-08-24 | 1994-03-10 | Akzo Nv | Method for nucleic acid amplification |
US5591575A (en) | 1993-04-07 | 1997-01-07 | Amersham International Plc | Subtraction hybridization employing aziridinylbenoquinone cross-linking agents |
US6096715A (en) | 1993-05-07 | 2000-08-01 | City Of Hope | Chimeric DNA-RNA catalytic sequences |
US6027923A (en) | 1993-07-23 | 2000-02-22 | Bio-Rad Laboratories, Inc. | Linked linear amplification of nucleic acids |
FR2708288B1 (en) | 1993-07-26 | 1995-09-01 | Bio Merieux | Method for amplification of nucleic acids by transcription using displacement, reagents and necessary for the implementation of this method. |
AU7487294A (en) | 1993-08-18 | 1995-03-14 | Id Biomedical Corporation | Compositions and methods for detecting target nucleic acid sequences utilizing flanking sequence enzyme molecules |
WO1995014106A2 (en) | 1993-11-17 | 1995-05-26 | Id Biomedical Corporation | Cycling probe cleavage detection of nucleic acid sequences |
US5578832A (en) | 1994-09-02 | 1996-11-26 | Affymetrix, Inc. | Method and apparatus for imaging a sample on a device |
US5648211A (en) | 1994-04-18 | 1997-07-15 | Becton, Dickinson And Company | Strand displacement amplification using thermophilic enzymes |
FR2724934B1 (en) * | 1994-09-26 | 1997-01-24 | Bio Merieux | CHIMERIC OLIGONUCLEOTIDE AND ITS USE IN OBTAINING NUCLEIC ACID TRANSCRIPTS |
US5556752A (en) | 1994-10-24 | 1996-09-17 | Affymetrix, Inc. | Surface-bound, unimolecular, double-stranded DNA |
US5665545A (en) | 1994-11-28 | 1997-09-09 | Akzo Nobel N.V. | Terminal repeat amplification method |
US5830655A (en) | 1995-05-22 | 1998-11-03 | Sri International | Oligonucleotide sizing using cleavable primers |
US5700642A (en) | 1995-05-22 | 1997-12-23 | Sri International | Oligonucleotide sizing using immobilized cleavable primers |
US5882867A (en) | 1995-06-07 | 1999-03-16 | Dade Behring Marburg Gmbh | Detection of nucleic acids by formation of template-dependent product |
US5916777A (en) | 1995-06-07 | 1999-06-29 | Gen-Probe Incorporated | Enzymatic synthesis of oligonucleotides using 3'-ribonucleotide primers |
US5932450A (en) | 1995-06-07 | 1999-08-03 | Gen-Probe Incorporated | Enzymatic synthesis of oligonucleotides using digestible templates |
US5763178A (en) | 1995-06-07 | 1998-06-09 | Trevigen, Inc. | Oscillating signal amplifier for nucleic acid detection |
JPH0920263A (en) | 1995-07-06 | 1997-01-21 | Jidosha Kiki Co Ltd | Motor-driven pump type power steering device |
FR2737223B1 (en) | 1995-07-24 | 1997-09-12 | Bio Merieux | METHOD OF AMPLIFYING NUCLEIC ACID SEQUENCES BY MOVEMENT USING CHIMERIC PRIMERS |
US5652356A (en) | 1995-08-17 | 1997-07-29 | Hybridon, Inc. | Inverted chimeric and hybrid oligonucleotides |
US5871697A (en) | 1995-10-24 | 1999-02-16 | Curagen Corporation | Method and apparatus for identifying, classifying, or quantifying DNA sequences in a sample without sequencing |
US5854033A (en) | 1995-11-21 | 1998-12-29 | Yale University | Rolling circle replication reporter systems |
AU714486B2 (en) | 1995-11-21 | 2000-01-06 | Yale University | Unimolecular segment amplification and detection |
WO1997021821A1 (en) | 1995-12-15 | 1997-06-19 | Amersham Life Science, Inc. | Thermostable dna polymerase from thermoanaerobacter thermohydrosulfuricus and mutant enzymes thereof with exonuclease activity removed |
US5962271A (en) | 1996-01-03 | 1999-10-05 | Cloutech Laboratories, Inc. | Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end |
EP0912761A4 (en) | 1996-05-29 | 2004-06-09 | Cornell Res Foundation Inc | Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions |
US5858665A (en) | 1996-07-25 | 1999-01-12 | Navix, Inc. | Homogeneous diagnostic assay method utilizing simultaneous target and signal amplification |
US5853990A (en) | 1996-07-26 | 1998-12-29 | Edward E. Winger | Real time homogeneous nucleotide assay |
JP2000516468A (en) | 1996-08-14 | 2000-12-12 | ライフ テクノロジーズ,インコーポレイテッド | Stable compositions for nucleic acid amplification and sequencing |
DE19653439A1 (en) | 1996-12-20 | 1998-07-02 | Svante Dr Paeaebo | Methods for the direct, exponential amplification and sequencing of DNA molecules and their application |
US6136533A (en) | 1997-07-03 | 2000-10-24 | Id Biomedical | Additives for use in cycling probe reactions |
US5932451A (en) * | 1997-11-19 | 1999-08-03 | Incyte Pharmaceuticals, Inc. | Method for unbiased mRNA amplification |
US20010000077A1 (en) | 1998-02-03 | 2001-03-29 | Engelhardt Dean L. | Novel process, construct and conjugate for producing multiple nucleic acid copies |
US6743605B1 (en) | 1998-06-24 | 2004-06-01 | Enzo Life Sciences, Inc. | Linear amplification of specific nucleic acid sequences |
US6358712B1 (en) | 1999-01-05 | 2002-03-19 | Trustee Of Boston University | Ordered gene assembly |
US6951722B2 (en) | 1999-03-19 | 2005-10-04 | Takara Bio Inc. | Method for amplifying nucleic acid sequence |
US6355431B1 (en) | 1999-04-20 | 2002-03-12 | Illumina, Inc. | Detection of nucleic acid amplification reactions using bead arrays |
WO2000070095A2 (en) * | 1999-05-17 | 2000-11-23 | Dade Behring Inc. | Homogeneous isothermal amplification and detection of nucleic acids using a template switch oligonucleotide |
US6132997A (en) | 1999-05-28 | 2000-10-17 | Agilent Technologies | Method for linear mRNA amplification |
US6692918B2 (en) | 1999-09-13 | 2004-02-17 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
US6271002B1 (en) * | 1999-10-04 | 2001-08-07 | Rosetta Inpharmatics, Inc. | RNA amplification method |
ATE455186T1 (en) * | 2000-06-26 | 2010-01-15 | Nugen Technologies Inc | METHODS AND COMPOSITIONS FOR TRANSCRIPTION-BASED DUPLICATION OF NUCLEIC ACIDS |
US6596490B2 (en) | 2000-07-14 | 2003-07-22 | Applied Gene Technologies, Inc. | Nucleic acid hairpin probes and uses thereof |
WO2002029117A2 (en) | 2000-10-06 | 2002-04-11 | Nugen Technologies, Inc. | Methods and probes for detection and/or quantification of nucleic acid sequences |
CA2427319A1 (en) | 2000-10-30 | 2002-12-27 | Gene Logic, Inc. | Partially double-stranded nucleic acids, methods of making, and use thereof |
US6858413B2 (en) | 2000-12-13 | 2005-02-22 | Nugen Technologies, Inc. | Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof |
ATE361996T1 (en) | 2001-03-09 | 2007-06-15 | Nugen Technologies Inc | METHODS AND COMPOSITIONS FOR DUPLICATION OF RNA SEQUENCES |
JP2005508135A (en) * | 2001-03-09 | 2005-03-31 | ニューゲン テクノロジーズ, インコーポレイテッド | Methods and compositions for amplification of RNA sequences |
EP1488001B1 (en) | 2002-03-11 | 2008-08-20 | Nugen Technologies, Inc. | Methods for generating double stranded dna comprising a 3' single stranded portion and uses of these complexes for recombination |
EP1490514A4 (en) | 2002-03-29 | 2008-12-17 | Nugen Technologies Inc | Single primer isothermal nucleic acid amplification-enhanced analyte detection and quantification |
US20040005614A1 (en) | 2002-05-17 | 2004-01-08 | Nurith Kurn | Methods for fragmentation, labeling and immobilization of nucleic acids |
AU2004230494A1 (en) | 2003-04-14 | 2004-10-28 | Nugen Technologies, Inc. | Global amplification using a randomly primed composite primer |
-
2002
- 2002-03-11 JP JP2002571829A patent/JP2005508135A/en active Pending
- 2002-03-11 US US10/096,221 patent/US7094536B2/en not_active Expired - Fee Related
- 2002-03-11 WO PCT/US2002/007377 patent/WO2002072773A2/en active Search and Examination
- 2002-03-11 CA CA002439074A patent/CA2439074A1/en not_active Abandoned
- 2002-03-11 EP EP02731119.0A patent/EP1390537B1/en not_active Expired - Lifetime
-
2005
- 2005-06-08 US US11/148,593 patent/US7351557B2/en not_active Expired - Fee Related
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6251639B1 (en) * | 1999-09-13 | 2001-06-26 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences, using a RNA-DNA composite primer |
Non-Patent Citations (1)
Title |
---|
See also references of EP1390537A4 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8143001B2 (en) | 2003-12-29 | 2012-03-27 | Nugen Technologies, Inc. | Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids |
US9206418B2 (en) | 2011-10-19 | 2015-12-08 | Nugen Technologies, Inc. | Compositions and methods for directional nucleic acid amplification and sequencing |
Also Published As
Publication number | Publication date |
---|---|
US7351557B2 (en) | 2008-04-01 |
EP1390537B1 (en) | 2013-11-13 |
WO2002072773A2 (en) | 2002-09-19 |
US20020164628A1 (en) | 2002-11-07 |
EP1390537A4 (en) | 2006-04-26 |
CA2439074A1 (en) | 2002-09-19 |
US20060014182A1 (en) | 2006-01-19 |
JP2005508135A (en) | 2005-03-31 |
EP1390537A2 (en) | 2004-02-25 |
US7094536B2 (en) | 2006-08-22 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
WO2002072773A3 (en) | Methods and compositions for amplification of rna sequences | |
WO2002072772A3 (en) | Methods and compositions for amplification of rna sequences | |
WO2005042759A3 (en) | Expression profiling using microarrays | |
WO2003010328A3 (en) | Complexity management of genomic dna | |
WO2009117698A3 (en) | Methods of rna amplification in the presence of dna | |
WO2002052031A3 (en) | Nucleic acid amplification | |
US20090325183A1 (en) | Sequencing methods | |
WO2006102309A3 (en) | Methods, compositions, and kits for detection of micro rna | |
ATE358733T1 (en) | RAPID METHOD FOR DETECTING MICROORGANISMS IN FOOD SAMPLES | |
WO2006026388A3 (en) | Single-primer nucleic acid amplification methods | |
WO2004081228A3 (en) | Real time gene expression profiling | |
WO2003054209A3 (en) | A method of reducing non-specific amplification in pcr | |
AU7104300A (en) | Method for amplifying signal-flanking sequences from unknown genomic dna | |
WO2005003386A3 (en) | Oligonucleotide ligation assay by detecting released pyrophosphate | |
WO2006138183A3 (en) | Multiplexed polymerase chain reaction for genetic sequence analysis | |
EP3378948B1 (en) | Method for quantifying target nucleic acid and kit therefor | |
EP1862558A4 (en) | Gene detection method | |
WO2003060159A3 (en) | Methods of nucleic acid amplification | |
EP1323835A3 (en) | Method for determining biospecies contained in test specimen and kit used for the same | |
WO2004097003A3 (en) | Multiply-primed amplification of nucleic acid sequences | |
ATE438849T1 (en) | METHOD FOR DETECTING STAPHYLOCOCCUS EPIDERMIDIS | |
EP0872561A3 (en) | Neisseria gonorrhoae-specific oligonucleotides | |
EP2173909A1 (en) | Target preparation for parallel sequencing of complex genomes | |
WO2002059348A3 (en) | Methods for determining the genetic affinity of microorganisms and viruses | |
AU2002312804A1 (en) | Method for detecting gram-positive bacteria having a high gc content |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AK | Designated states |
Kind code of ref document: A2 Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SD SE SG SI SK SL TJ TM TN TR TT TZ UA UG US UZ VN YU ZA ZM ZW |
|
AL | Designated countries for regional patents |
Kind code of ref document: A2 Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG |
|
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
WWE | Wipo information: entry into national phase |
Ref document number: 2002731119 Country of ref document: EP Ref document number: 2439074 Country of ref document: CA |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2002303118 Country of ref document: AU |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2002571829 Country of ref document: JP |
|
DFPE | Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101) | ||
REG | Reference to national code |
Ref country code: DE Ref legal event code: 8642 |
|
WWP | Wipo information: published in national office |
Ref document number: 2002731119 Country of ref document: EP |