WO2002086051A3 - Nucleic acid analysis by mass differentiable oligomers - Google Patents

Nucleic acid analysis by mass differentiable oligomers Download PDF

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Publication number
WO2002086051A3
WO2002086051A3 PCT/IB2002/002298 IB0202298W WO02086051A3 WO 2002086051 A3 WO2002086051 A3 WO 2002086051A3 IB 0202298 W IB0202298 W IB 0202298W WO 02086051 A3 WO02086051 A3 WO 02086051A3
Authority
WO
WIPO (PCT)
Prior art keywords
mass
oligomers
monomers
differentiable
nucleic acid
Prior art date
Application number
PCT/IB2002/002298
Other languages
French (fr)
Other versions
WO2002086051A2 (en
Inventor
Frank Grosveld
Original Assignee
Univ Erasmus
Frank Grosveld
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Erasmus, Frank Grosveld filed Critical Univ Erasmus
Priority to CA002445248A priority Critical patent/CA2445248A1/en
Priority to EP02735871A priority patent/EP1385932A2/en
Priority to JP2002583567A priority patent/JP2005500824A/en
Priority to BR0209205-0A priority patent/BR0209205A/en
Publication of WO2002086051A2 publication Critical patent/WO2002086051A2/en
Priority to US10/693,308 priority patent/US20040137570A1/en
Publication of WO2002086051A3 publication Critical patent/WO2002086051A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Abstract

The invention comprises a method for constructing a repertoire of oligomers differentiable by mass, providing a heterogeneous pool of monomers, wherein said monomers are modified by addition of one or more of a selection of mass labels; optionally, providing a heterogeneous pool of unlabelled monomers; determining the monomer sequences of the oligomers to be represented in the repertoire and calculating the number and nature of the mass labels to be incorporated into each monomer such that each oligomer differs in mass; and assembling a plurality of labelled monomers and, optionally, one or more unlabelled monomers, to form the oligomers.
PCT/IB2002/002298 2001-04-24 2002-04-24 Nucleic acid analysis by mass differentiable oligomers WO2002086051A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CA002445248A CA2445248A1 (en) 2001-04-24 2002-04-24 Nucleic acid analysis by mass differentiable oligomers
EP02735871A EP1385932A2 (en) 2001-04-24 2002-04-24 Nucleic acid analysis by mass differentiable oligomers
JP2002583567A JP2005500824A (en) 2001-04-24 2002-04-24 Nucleic acid analysis
BR0209205-0A BR0209205A (en) 2001-04-24 2002-04-24 Nucleic acid analysis
US10/693,308 US20040137570A1 (en) 2001-04-24 2003-10-24 Immunoglobulin 2

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0110030.4A GB0110030D0 (en) 2001-04-24 2001-04-24 RNA genomics
GB0110030.4 2001-04-24

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US10/693,308 Continuation US20040137570A1 (en) 2001-04-24 2003-10-24 Immunoglobulin 2

Publications (2)

Publication Number Publication Date
WO2002086051A2 WO2002086051A2 (en) 2002-10-31
WO2002086051A3 true WO2002086051A3 (en) 2003-11-20

Family

ID=9913360

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2002/002298 WO2002086051A2 (en) 2001-04-24 2002-04-24 Nucleic acid analysis by mass differentiable oligomers

Country Status (6)

Country Link
EP (1) EP1385932A2 (en)
JP (1) JP2005500824A (en)
BR (1) BR0209205A (en)
CA (1) CA2445248A1 (en)
GB (1) GB0110030D0 (en)
WO (1) WO2002086051A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0423873D0 (en) * 2004-10-27 2004-12-01 Univ Erasmus Nucleic acid analysis
CN102169118B (en) * 2011-04-15 2012-01-25 华瑞同康生物技术（深圳）有限公司 Method for detecting 8-oxo-deoxyguanosine (8-oxo-dG)
US20140221223A1 (en) * 2012-12-10 2014-08-07 Advandx, Inc. Use of Probes for Mass Spectrometric Identification and Resistance Determination of Microorganisms or Cells

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999002728A1 (en) * 1997-07-11 1999-01-21 Brax Group Limited Characterising nucleic acids
WO1999045150A1 (en) * 1998-03-02 1999-09-10 Isis Pharmaceuticals, Inc. Mass spectrometric methods for biomolecular screening
WO1999057318A2 (en) * 1998-05-07 1999-11-11 Sequenom, Inc. Infrared matrix-assisted laser desorption/ionization mass spectrometric analysis of macromolecules
US6027890A (en) * 1996-01-23 2000-02-22 Rapigene, Inc. Methods and compositions for enhancing sensitivity in the analysis of biological-based assays
US6194144B1 (en) * 1993-01-07 2001-02-27 Sequenom, Inc. DNA sequencing by mass spectrometry

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6194144B1 (en) * 1993-01-07 2001-02-27 Sequenom, Inc. DNA sequencing by mass spectrometry
US6027890A (en) * 1996-01-23 2000-02-22 Rapigene, Inc. Methods and compositions for enhancing sensitivity in the analysis of biological-based assays
WO1999002728A1 (en) * 1997-07-11 1999-01-21 Brax Group Limited Characterising nucleic acids
WO1999045150A1 (en) * 1998-03-02 1999-09-10 Isis Pharmaceuticals, Inc. Mass spectrometric methods for biomolecular screening
WO1999057318A2 (en) * 1998-05-07 1999-11-11 Sequenom, Inc. Infrared matrix-assisted laser desorption/ionization mass spectrometric analysis of macromolecules

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ISOLA ET AL.: "MALDI-TOF mass spectrometric methods for detection of hybridized DNA oligomers", ANAL. CHEM., vol. 73, 1 May 2001 (2001-05-01), pages 2126 - 2131, XP002239467 *

Also Published As

Publication number Publication date
CA2445248A1 (en) 2002-10-31
EP1385932A2 (en) 2004-02-04
WO2002086051A2 (en) 2002-10-31
GB0110030D0 (en) 2001-06-13
JP2005500824A (en) 2005-01-13
BR0209205A (en) 2005-12-13

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