WO2002086140A3 - Double-strand nucleic acids with one or two individual strand 5' overhangs, method for production and use thereof - Google Patents

Double-strand nucleic acids with one or two individual strand 5' overhangs, method for production and use thereof Download PDF

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Publication number
WO2002086140A3
WO2002086140A3 PCT/EP2002/004358 EP0204358W WO02086140A3 WO 2002086140 A3 WO2002086140 A3 WO 2002086140A3 EP 0204358 W EP0204358 W EP 0204358W WO 02086140 A3 WO02086140 A3 WO 02086140A3
Authority
WO
WIPO (PCT)
Prior art keywords
strand
production
overhangs
nucleic acids
double
Prior art date
Application number
PCT/EP2002/004358
Other languages
German (de)
French (fr)
Other versions
WO2002086140A2 (en
Inventor
Rolf Postina
Original Assignee
Rolf Postina
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rolf Postina filed Critical Rolf Postina
Priority to AU2002315302A priority Critical patent/AU2002315302A1/en
Publication of WO2002086140A2 publication Critical patent/WO2002086140A2/en
Publication of WO2002086140A3 publication Critical patent/WO2002086140A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Abstract

The invention relates to novel double-strand nucleic acids with individual strand overhangs, method for production and use thereof in recombinant reactions.
PCT/EP2002/004358 2001-04-19 2002-04-19 Double-strand nucleic acids with one or two individual strand 5' overhangs, method for production and use thereof WO2002086140A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002315302A AU2002315302A1 (en) 2001-04-19 2002-04-19 Double-strand nucleic acids with one or two individual strand 5' overhangs, method for production and use thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10119203.7 2001-04-19
DE2001119203 DE10119203B4 (en) 2001-04-19 2001-04-19 Process for the production of double-stranded nucleic acids with single-stranded overhangs of any length and nucleotide composition

Publications (2)

Publication Number Publication Date
WO2002086140A2 WO2002086140A2 (en) 2002-10-31
WO2002086140A3 true WO2002086140A3 (en) 2003-08-28

Family

ID=7681979

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/004358 WO2002086140A2 (en) 2001-04-19 2002-04-19 Double-strand nucleic acids with one or two individual strand 5' overhangs, method for production and use thereof

Country Status (3)

Country Link
AU (1) AU2002315302A1 (en)
DE (1) DE10119203B4 (en)
WO (1) WO2002086140A2 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4521509A (en) * 1982-11-24 1985-06-04 Research Corporation Method for degrading DNA
EP0549107A1 (en) * 1991-10-11 1993-06-30 BEHRINGWERKE Aktiengesellschaft Method for producing a polynucleotide for use in single primer amplification and phosphorothioate-containing oligonucleotides as primers in nucleic acid amplification
US5518900A (en) * 1993-01-15 1996-05-21 Molecular Tool, Inc. Method for generating single-stranded DNA molecules

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4372837B2 (en) * 1992-08-04 2009-11-25 ジェン−プローブ・インコーポレイテッド Nucleic acid sequence amplification

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4521509A (en) * 1982-11-24 1985-06-04 Research Corporation Method for degrading DNA
EP0549107A1 (en) * 1991-10-11 1993-06-30 BEHRINGWERKE Aktiengesellschaft Method for producing a polynucleotide for use in single primer amplification and phosphorothioate-containing oligonucleotides as primers in nucleic acid amplification
US5518900A (en) * 1993-01-15 1996-05-21 Molecular Tool, Inc. Method for generating single-stranded DNA molecules

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
COLJEE ET AL: "Seamless gene engineering using RNA- and DNA- overhang cloning", NATURE BIOTECHNOLOGY, NATURE PUBLISHING, US, vol. 18, July 2000 (2000-07-01), pages 789 - 791, XP002142431, ISSN: 1087-0156 *
GAL J ET AL: "DIRECTIONAL CLONING OF NATIVE PCR PRODUCTS WITH PREFORMED STICKY ENDS (AUTOSTICKY PCR)", MOL. GEN. GENET., vol. 260, no. 6, January 1999 (1999-01-01), pages 569 - 573, XP000867543 *
GAL JOZSEF ET AL: "Polymerase dependence of Autosticky polymerase chain reaction.", ANALYTICAL BIOCHEMISTRY, vol. 282, no. 1, 15 June 2000 (2000-06-15), pages 156 - 158, XP002240192, ISSN: 0003-2697 *
NEWTON C R ET AL: "THE PRODUCTION OF PCR PRODUCTS WITH 5' SINGLE-STRANDED TAILS USING PRIMERS THAT INCORPORATE NOVEL PHOSPHORAMIDITE INTERMEDIATES", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 21, no. 5, 1993, pages 1155 - 1162, XP002041613, ISSN: 0305-1048 *
SKERRA A: "PHOSPHOROTHIOATE PRIMERS IMPROVE THE AMPLICATION OF DNA SEQUENCES BY DNA POLYMERASES WITH PROOFREADING ACTIVITY", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 20, no. 14, 25 July 1992 (1992-07-25), pages 3551 - 3554, XP000363599, ISSN: 0305-1048 *
THALER DAVID S ET AL: "Extending the chemistry that supports genetic information transfer in vivo: Phosphorothioate DNA, phosphorothioate RNA, 2'-O-methyl RNA, and methylphosphonate DNA.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 93, no. 3, 1996, 1996, pages 1352 - 1356, XP002240191, ISSN: 0027-8424 *

Also Published As

Publication number Publication date
WO2002086140A2 (en) 2002-10-31
DE10119203A1 (en) 2002-11-28
DE10119203B4 (en) 2004-09-16
AU2002315302A1 (en) 2002-11-05

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