WO2002094998A3 - Analyzing phosphorylated proteins - Google Patents

Analyzing phosphorylated proteins Download PDF

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Publication number
WO2002094998A3
WO2002094998A3 PCT/US2002/016100 US0216100W WO02094998A3 WO 2002094998 A3 WO2002094998 A3 WO 2002094998A3 US 0216100 W US0216100 W US 0216100W WO 02094998 A3 WO02094998 A3 WO 02094998A3
Authority
WO
WIPO (PCT)
Prior art keywords
target
cleavage
target polypeptide
polypeptides
polypeptide
Prior art date
Application number
PCT/US2002/016100
Other languages
French (fr)
Other versions
WO2002094998A2 (en
WO2002094998A9 (en
Inventor
Sharat Singh
Robert A Zivin
Original Assignee
Aclara Biosciences Inc
Johnson And Johnson Pharmaceut
Sharat Singh
Robert A Zivin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aclara Biosciences Inc, Johnson And Johnson Pharmaceut, Sharat Singh, Robert A Zivin filed Critical Aclara Biosciences Inc
Priority to AU2002305658A priority Critical patent/AU2002305658A1/en
Publication of WO2002094998A2 publication Critical patent/WO2002094998A2/en
Publication of WO2002094998A9 publication Critical patent/WO2002094998A9/en
Publication of WO2002094998A3 publication Critical patent/WO2002094998A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/807—Apparatus included in process claim, e.g. physical support structures
    • Y10S436/808—Automated or kit

Abstract

Methods, compositions and kits are disclosed for determining one or more target polypeptides in a sample where the target polypeptides have undergone phosphorylation. A mixture comprising the sample and a first reagent comprising a cleavage-inducing moiety and an IMAC resin for a binding site on a target polypeptide is subjected to conditions under which binding of respective binding moieties occurs. The binding site is the result of phosphorylation activity involving the target polypeptide. The method may be employed to determine the target polypeptide itself. In another embodiment the presence and/or amount of the target polypeptide is related to the presence and/or amount and/or activity of an agent such as an enzyme involved in phosphorylation of the target polypeptide. The interaction between the IMAC resin and the binding site brings the cleavage-inducing moiety into close proximity to a cleavable moiety, which is associated with the polypeptide and is susceptible to cleavage only when in proximity to the cleavage-inducing moiety. In this way, an electrophoretic tag for each of the polypeptides may be released. Released electrophoretic tags are separated and the presence and/or amount of the target polypeptides are determined based on the corresponding electrophoretic tags.
PCT/US2002/016100 2001-05-21 2002-05-21 Analyzing phosphorylated proteins WO2002094998A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002305658A AU2002305658A1 (en) 2001-05-21 2002-05-21 Analyzing phosphorylated proteins

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US29254801P 2001-05-21 2001-05-21
US60/292,548 2001-05-21
US33490201P 2001-10-24 2001-10-24
US60/334,902 2001-10-24

Publications (3)

Publication Number Publication Date
WO2002094998A2 WO2002094998A2 (en) 2002-11-28
WO2002094998A9 WO2002094998A9 (en) 2003-10-09
WO2002094998A3 true WO2002094998A3 (en) 2004-02-05

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/016100 WO2002094998A2 (en) 2001-05-21 2002-05-21 Analyzing phosphorylated proteins

Country Status (4)

Country Link
US (1) US7041459B2 (en)
AU (1) AU2002305658A1 (en)
HU (1) HUP0501021A3 (en)
WO (1) WO2002094998A2 (en)

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Also Published As

Publication number Publication date
US7041459B2 (en) 2006-05-09
WO2002094998A2 (en) 2002-11-28
US20030040016A1 (en) 2003-02-27
HUP0501021A3 (en) 2006-06-28
HUP0501021A2 (en) 2006-02-28
WO2002094998A9 (en) 2003-10-09
AU2002305658A1 (en) 2002-12-03

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