WO2003001870A3 - Methods for making antibody fragments and compositions resulting therefrom - Google Patents

Methods for making antibody fragments and compositions resulting therefrom Download PDF

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Publication number
WO2003001870A3
WO2003001870A3 PCT/US2002/018573 US0218573W WO03001870A3 WO 2003001870 A3 WO2003001870 A3 WO 2003001870A3 US 0218573 W US0218573 W US 0218573W WO 03001870 A3 WO03001870 A3 WO 03001870A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
antibody fragments
compositions resulting
making antibody
resulting therefrom
Prior art date
Application number
PCT/US2002/018573
Other languages
French (fr)
Other versions
WO2003001870A2 (en
Inventor
Steffen Nock
David S Wilson
Jiangchun Wu
Original Assignee
Zyomyx Inc
Steffen Nock
David S Wilson
Jiangchun Wu
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zyomyx Inc, Steffen Nock, David S Wilson, Jiangchun Wu filed Critical Zyomyx Inc
Priority to AU2002315066A priority Critical patent/AU2002315066A1/en
Publication of WO2003001870A2 publication Critical patent/WO2003001870A2/en
Publication of WO2003001870A3 publication Critical patent/WO2003001870A3/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065Purification, fragmentation
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/40Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41Glycosylation, sialylation, or fucosylation
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/50Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/54F(ab')2
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/866Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof involving immunoglobulin or antibody fragment, e.g. fab', fab, fv, fc, heavy chain or light chain

Abstract

Improved methods for making antibody fragments, preferably F(ab')2 fragments from various classes and subclasses of antibodies is described. Pretreatment of antibodies with deglycosylases or cellular inhibition of glycosylation during expression, yields antibodies having improved susceptibility towards protease cleavage, preferably pepsinolysis, which yields F(ab')2 antibody fragments. Compositions resulting from such methods are further disclosed.
PCT/US2002/018573 2001-06-14 2002-06-12 Methods for making antibody fragments and compositions resulting therefrom WO2003001870A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002315066A AU2002315066A1 (en) 2001-06-14 2002-06-12 Methods for making antibody fragments and compositions resulting therefrom

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/882,814 2001-06-14
US09/882,814 US6720165B2 (en) 2001-06-14 2001-06-14 Methods for making antibody fragments and compositions resulting therefrom

Publications (2)

Publication Number Publication Date
WO2003001870A2 WO2003001870A2 (en) 2003-01-09
WO2003001870A3 true WO2003001870A3 (en) 2003-07-31

Family

ID=25381391

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/018573 WO2003001870A2 (en) 2001-06-14 2002-06-12 Methods for making antibody fragments and compositions resulting therefrom

Country Status (3)

Country Link
US (2) US6720165B2 (en)
AU (1) AU2002315066A1 (en)
WO (1) WO2003001870A2 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040253659A1 (en) * 2003-06-11 2004-12-16 Michele Rasamoelisolo Method for identification of glycosylated polypeptides
JP4667383B2 (en) * 2003-06-13 2011-04-13 バイオジェン・アイデック・エムエイ・インコーポレイテッド Aglycosyl anti-CD154 (CD40 ligand) antibody and use thereof
AU2004266159A1 (en) 2003-08-22 2005-03-03 Biogen Idec Ma Inc. Improved antibodies having altered effector function and methods for making the same
KR20070047327A (en) * 2004-07-26 2007-05-04 비오겐 아이덱 엠에이 아이엔씨. Anti-cd154 antibodies
US20060073534A1 (en) * 2004-10-05 2006-04-06 The University Of Georgia Research Foundation, Inc Method for cleaving and deglycosylating antibodies to promote ligand binding
JP5137016B2 (en) * 2005-11-30 2013-02-06 学校法人日本大学 Ultrasensitive C-reactive protein measuring reagent and measuring method
DE602007008872D1 (en) * 2006-05-02 2010-10-14 Genentech Inc MICROWAVE-BASED DEGLYCOSYLATION OF PROTEINS FOR MOLECULAR WEIGHT DETERMINATION BY MASS SPECTROMETRY
WO2009046168A1 (en) 2007-10-02 2009-04-09 Avaxia Biologics, Inc. Antibody therapy for use in the digestive tract
HU0900195D0 (en) * 2009-04-01 2009-06-29 Debreceni Egyetem Diagnosis and treatment of gluten-induced autoimmune diseases
JP2018513983A (en) * 2015-04-06 2018-05-31 ブルーダイアグノスティックス・インコーポレイテッドBludiagnostics, Inc. Test apparatus and method of use for detecting an analyte in a saliva sample
EP3374500A1 (en) * 2015-11-13 2018-09-19 Mammedov, Tarlan Production of in vivo n-deglycosylated recombinant proteins by co-expression with endo h
CA3063815A1 (en) 2017-05-26 2018-11-29 Genovis Ab Protease and binding polypeptide for o-glycoproteins
CN108445224A (en) * 2018-02-26 2018-08-24 王贤俊 A kind of cardic fatty acid binding protein antibody fragment compounded latex particle and preparation method thereof

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4849352A (en) * 1984-10-09 1989-07-18 Sullivan John B Antibody purification process
US5099005A (en) * 1990-09-28 1992-03-24 Neorx Corporation Method of enhancing immunoglobulin fragment production
US5585097A (en) * 1992-03-24 1996-12-17 British Technology Group Limited Humanized anti-CD3 specific antibodies
US6218149B1 (en) * 1988-09-15 2001-04-17 The Trustees Of Columbus University In The City Of New York Antibodies having modified carbohydrate content and methods of preparation and use
US20020150914A1 (en) * 1995-06-30 2002-10-17 Kobenhavns Universitet Recombinant antibodies from a phage display library, directed against a peptide-MHC complex

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4281061A (en) * 1979-07-27 1981-07-28 Syva Company Double antibody for enhanced sensitivity in immunoassay
GB2226269B (en) * 1988-12-22 1992-11-25 Zdzislaw Bieganski Cable stripper
JP4503706B2 (en) * 1996-06-07 2010-07-14 ポニアード ファーマシューティカルズ,インコーポレイティド Humanized antibodies with modified glycosylation

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4849352A (en) * 1984-10-09 1989-07-18 Sullivan John B Antibody purification process
US6218149B1 (en) * 1988-09-15 2001-04-17 The Trustees Of Columbus University In The City Of New York Antibodies having modified carbohydrate content and methods of preparation and use
US5099005A (en) * 1990-09-28 1992-03-24 Neorx Corporation Method of enhancing immunoglobulin fragment production
US5585097A (en) * 1992-03-24 1996-12-17 British Technology Group Limited Humanized anti-CD3 specific antibodies
US20020150914A1 (en) * 1995-06-30 2002-10-17 Kobenhavns Universitet Recombinant antibodies from a phage display library, directed against a peptide-MHC complex

Also Published As

Publication number Publication date
AU2002315066A1 (en) 2003-03-03
US6720165B2 (en) 2004-04-13
US20020193573A1 (en) 2002-12-19
US20040091957A1 (en) 2004-05-13
WO2003001870A2 (en) 2003-01-09

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