WO2003008977A3 - Arrays of buffers for analysing biomolecules by their isoelectric point - Google Patents

Arrays of buffers for analysing biomolecules by their isoelectric point Download PDF

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Publication number
WO2003008977A3
WO2003008977A3 PCT/US2002/022714 US0222714W WO03008977A3 WO 2003008977 A3 WO2003008977 A3 WO 2003008977A3 US 0222714 W US0222714 W US 0222714W WO 03008977 A3 WO03008977 A3 WO 03008977A3
Authority
WO
WIPO (PCT)
Prior art keywords
arrays
isoelectric point
buffers
matrixes
useful
Prior art date
Application number
PCT/US2002/022714
Other languages
French (fr)
Other versions
WO2003008977A2 (en
Inventor
Shamuel Bukshpan
Gleb Zilberstein
Original Assignee
Protein Forest Inc
Shamuel Bukshpan
Gleb Zilberstein
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Protein Forest Inc, Shamuel Bukshpan, Gleb Zilberstein filed Critical Protein Forest Inc
Priority to BRPI0211213-2A priority Critical patent/BR0211213A/en
Priority to CA002454045A priority patent/CA2454045A1/en
Priority to DE60222427T priority patent/DE60222427T2/en
Priority to AU2002322513A priority patent/AU2002322513B2/en
Priority to EP02756507A priority patent/EP1410029B1/en
Priority to JP2003514267A priority patent/JP4108037B2/en
Priority to IL15981002A priority patent/IL159810A0/en
Priority to DK02756507T priority patent/DK1410029T3/en
Publication of WO2003008977A2 publication Critical patent/WO2003008977A2/en
Publication of WO2003008977A3 publication Critical patent/WO2003008977A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44795—Isoelectric focusing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44747—Composition of gel or of carrier mixture

Abstract

The present invention relates to matrixes, arrays, systems and methods for analyzing biomolecules by their isoelectric point, optionally, in combination with a second dimension analysis. The assortment of matrixes, arrays and systems provided herein are useful for causing a biomolecule under the influence of an electrical field to accumulate into an IEF buffer that comprises a pH value that is the same as the isoelectric point of the biomolecule. The methods of this invention are useful for, e.g., research and diagnostic purposes.
PCT/US2002/022714 2001-07-16 2002-07-16 Arrays of buffers for analysing biomolecules by their isoelectric point WO2003008977A2 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
BRPI0211213-2A BR0211213A (en) 2001-07-16 2002-07-16 arrays, ordered arrangement of elements, systems and methods for biomolecule analysis
CA002454045A CA2454045A1 (en) 2001-07-16 2002-07-16 Arrays of buffers for analysing biomolecules by their isoelectric point
DE60222427T DE60222427T2 (en) 2001-07-16 2002-07-16 BUFFER ARRAYS FOR THE DETECTION OF BIOMOLECULES BASED ON ITS ISOELECTRIC POINT
AU2002322513A AU2002322513B2 (en) 2001-07-16 2002-07-16 Arrays of buffers for analysing biomolecules by their isoelectric point
EP02756507A EP1410029B1 (en) 2001-07-16 2002-07-16 Arrays of buffers for analysing biomolecules by their isoelectric point
JP2003514267A JP4108037B2 (en) 2001-07-16 2002-07-16 Array of buffers for analyzing biomolecules by their isoelectric points
IL15981002A IL159810A0 (en) 2001-07-16 2002-07-16 Matrixes, arrays, systems and methods
DK02756507T DK1410029T3 (en) 2001-07-16 2002-07-16 Arrays of buffers for analysis of biomolecules at their isoelectric point

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US30580201P 2001-07-16 2001-07-16
US60/305,802 2001-07-16
US31031601P 2001-08-06 2001-08-06
US60/310,316 2001-08-06
US34069801P 2001-10-29 2001-10-29
US60/340,698 2001-10-29
US37704402P 2002-04-30 2002-04-30
US60/377,044 2002-04-30

Publications (2)

Publication Number Publication Date
WO2003008977A2 WO2003008977A2 (en) 2003-01-30
WO2003008977A3 true WO2003008977A3 (en) 2003-06-19

Family

ID=27501888

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/022714 WO2003008977A2 (en) 2001-07-16 2002-07-16 Arrays of buffers for analysing biomolecules by their isoelectric point

Country Status (12)

Country Link
US (2) US7166202B2 (en)
EP (1) EP1410029B1 (en)
JP (1) JP4108037B2 (en)
CN (1) CN1549924A (en)
AT (1) ATE373236T1 (en)
AU (1) AU2002322513B2 (en)
BR (1) BR0211213A (en)
CA (1) CA2454045A1 (en)
DE (1) DE60222427T2 (en)
DK (1) DK1410029T3 (en)
IL (1) IL159810A0 (en)
WO (1) WO2003008977A2 (en)

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US20120190129A1 (en) * 2011-01-20 2012-07-26 Konica Minolta Holdings, Inc. Test device, reaction apparatus and reactive test method
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CN103412029B (en) * 2013-06-26 2018-10-16 华东理工大学 Tablet electrochromatography separation for amino acids device based on chip level and its application method
JP6791848B2 (en) * 2014-05-21 2020-11-25 アンチェインド ラブス Systems and methods for buffer replacement
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Also Published As

Publication number Publication date
IL159810A0 (en) 2004-06-20
CA2454045A1 (en) 2003-01-30
US7914656B2 (en) 2011-03-29
US7166202B2 (en) 2007-01-23
EP1410029B1 (en) 2007-09-12
US20070138015A1 (en) 2007-06-21
US20030102215A1 (en) 2003-06-05
AU2002322513B2 (en) 2008-02-28
DK1410029T3 (en) 2008-01-07
DE60222427T2 (en) 2008-06-12
ATE373236T1 (en) 2007-09-15
EP1410029A2 (en) 2004-04-21
CN1549924A (en) 2004-11-24
BR0211213A (en) 2006-05-23
DE60222427D1 (en) 2007-10-25
JP4108037B2 (en) 2008-06-25
JP2005526949A (en) 2005-09-08
WO2003008977A2 (en) 2003-01-30

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