WO2003025160A3 - Neuron culture medium and use of cholesterol for cultivating, differentiating or proliferating neurons - Google Patents

Neuron culture medium and use of cholesterol for cultivating, differentiating or proliferating neurons Download PDF

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Publication number
WO2003025160A3
WO2003025160A3 PCT/EP2002/010397 EP0210397W WO03025160A3 WO 2003025160 A3 WO2003025160 A3 WO 2003025160A3 EP 0210397 W EP0210397 W EP 0210397W WO 03025160 A3 WO03025160 A3 WO 03025160A3
Authority
WO
WIPO (PCT)
Prior art keywords
cholesterol
differentiating
cultivating
culture medium
neuron culture
Prior art date
Application number
PCT/EP2002/010397
Other languages
German (de)
French (fr)
Other versions
WO2003025160A2 (en
Inventor
Frank W Pfrieger
Karl Naegler
Original Assignee
Max Delbrueck Centrum
Frank W Pfrieger
Karl Naegler
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE10204747A external-priority patent/DE10204747A1/en
Application filed by Max Delbrueck Centrum, Frank W Pfrieger, Karl Naegler filed Critical Max Delbrueck Centrum
Publication of WO2003025160A2 publication Critical patent/WO2003025160A2/en
Publication of WO2003025160A3 publication Critical patent/WO2003025160A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0619—Neurons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/36—Lipids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
    • C12N2501/392—Sexual steroids

Abstract

The invention relates to a glia cell- and/or serum-free culture medium that is comprised of cholesterol, cholesterol derivatives, cholesterol analogues, cholesterol precursors and/or cholesterol-promoting substances. The invention further relates to the use of cholesterol, cholesterol derivatives, cholesterol analogues, cholesterol precursors and/or cholesterol-promoting substances for cultivating, allowing the survival of, proliferating, differentiating and/or growing neurons.
PCT/EP2002/010397 2001-09-14 2002-09-16 Neuron culture medium and use of cholesterol for cultivating, differentiating or proliferating neurons WO2003025160A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE10145400 2001-09-14
DE10145400.7 2001-09-14
DE10204747A DE10204747A1 (en) 2001-09-14 2002-02-06 New medium for the cultivation of nerve cells
DE10204747.2 2002-02-06

Publications (2)

Publication Number Publication Date
WO2003025160A2 WO2003025160A2 (en) 2003-03-27
WO2003025160A3 true WO2003025160A3 (en) 2003-09-25

Family

ID=26010141

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/010397 WO2003025160A2 (en) 2001-09-14 2002-09-16 Neuron culture medium and use of cholesterol for cultivating, differentiating or proliferating neurons

Country Status (1)

Country Link
WO (1) WO2003025160A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001011011A2 (en) * 1999-08-05 2001-02-15 Mcl Llc Multipotent adult stem cells and methods for isolation
WO2001042421A2 (en) * 1999-12-07 2001-06-14 Monash University Long-term cell culture compositions and genetically modified animals derived therefrom

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001011011A2 (en) * 1999-08-05 2001-02-15 Mcl Llc Multipotent adult stem cells and methods for isolation
WO2001042421A2 (en) * 1999-12-07 2001-06-14 Monash University Long-term cell culture compositions and genetically modified animals derived therefrom

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
MAUCH DANIELA H ET AL: "CNS synaptogenesis promoted by glia-derived cholesterol.", SCIENCE (WASHINGTON D C), vol. 294, no. 5545, 2001, pages 1354 - 1357, XP002236661, ISSN: 0036-8075 *
NAEGLER KARL ET AL: "Glia-derived signals induce synapse formation in neurones of the rat central nervous system.", JOURNAL OF PHYSIOLOGY (CAMBRIDGE), vol. 533, no. 3, 15 June 2001 (2001-06-15), pages 665 - 679, XP002236660, ISSN: 0022-3751 *

Also Published As

Publication number Publication date
WO2003025160A2 (en) 2003-03-27

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