WO2003033724A3 - Nucleic acid amplification - Google Patents

Nucleic acid amplification Download PDF

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Publication number
WO2003033724A3
WO2003033724A3 PCT/US2002/033244 US0233244W WO03033724A3 WO 2003033724 A3 WO2003033724 A3 WO 2003033724A3 US 0233244 W US0233244 W US 0233244W WO 03033724 A3 WO03033724 A3 WO 03033724A3
Authority
WO
WIPO (PCT)
Prior art keywords
primers
modified nucleotide
replication
nucleic acid
target
Prior art date
Application number
PCT/US2002/033244
Other languages
French (fr)
Other versions
WO2003033724A2 (en
Inventor
Frank B Dean
Roger S Lasken
Linhua Fang
Fawad A Faruqi
Osama A Alsmadi
Mark D Driscoll
Seiyu Hosono
Michele Wisniewski
Wanmin Song
Original Assignee
Molecular Staging Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/977,868 external-priority patent/US6977148B2/en
Application filed by Molecular Staging Inc filed Critical Molecular Staging Inc
Priority to EP02801776.2A priority Critical patent/EP1444355B1/en
Priority to CA002463933A priority patent/CA2463933A1/en
Priority to ES02801776.2T priority patent/ES2622733T3/en
Publication of WO2003033724A2 publication Critical patent/WO2003033724A2/en
Publication of WO2003033724A3 publication Critical patent/WO2003033724A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Abstract

Disclosed are compositions and a method for amplification of nucleic acid sequences of interest. The disclosed method generally involves replication of a target sequence such that, during replication, the replicated strands are displaced from the target sequence by strand displacement replication of another replicated strand. In one form of the disclosed method, the target sample is not subjected to denaturing conditions. It was discovered that the target nucleic acids, genomic DNA, for example, need not be denatured for efficient multiple displacement amplification. The primers used can be hexamer primers. The primers can also each contain at least one modified nucleotide such that the primers are nuclease resistant. The primers can also each contain at least one modified nucleotide such that the melting temperature of the primer is altered relative to a primer of the same sequence without the modified nucleotide(s). The DNA polymerase can be ϕ29 DNA polymerase.
PCT/US2002/033244 2001-10-15 2002-10-15 Nucleic acid amplification WO2003033724A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP02801776.2A EP1444355B1 (en) 2001-10-15 2002-10-15 Nucleic acid amplification
CA002463933A CA2463933A1 (en) 2001-10-15 2002-10-15 Nucleic acid amplification
ES02801776.2T ES2622733T3 (en) 2001-10-15 2002-10-15 Nucleic acid amplification

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US09/977,868 2001-10-15
US09/977,868 US6977148B2 (en) 2001-10-15 2001-10-15 Multiple displacement amplification
US09/982,212 2001-10-18
US09/982,212 US6617137B2 (en) 2001-10-15 2001-10-18 Method of amplifying whole genomes without subjecting the genome to denaturing conditions

Publications (2)

Publication Number Publication Date
WO2003033724A2 WO2003033724A2 (en) 2003-04-24
WO2003033724A3 true WO2003033724A3 (en) 2004-04-29

Family

ID=27130595

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/033244 WO2003033724A2 (en) 2001-10-15 2002-10-15 Nucleic acid amplification

Country Status (5)

Country Link
US (2) US6617137B2 (en)
EP (2) EP1724362A1 (en)
CA (1) CA2463933A1 (en)
ES (1) ES2622733T3 (en)
WO (1) WO2003033724A2 (en)

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US6617137B2 (en) 2003-09-09
US20030118998A1 (en) 2003-06-26
US20030143587A1 (en) 2003-07-31
EP1444355A4 (en) 2007-05-09
ES2622733T3 (en) 2017-07-07
US7074600B2 (en) 2006-07-11
EP1444355B1 (en) 2017-01-18
CA2463933A1 (en) 2003-04-24
WO2003033724A2 (en) 2003-04-24
EP1444355A2 (en) 2004-08-11
EP1724362A1 (en) 2006-11-22

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