WO2003054516A3 - Devices and methods for isolating and detecting specific substances in complex matrices - Google Patents

Devices and methods for isolating and detecting specific substances in complex matrices Download PDF

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Publication number
WO2003054516A3
WO2003054516A3 PCT/US2002/040991 US0240991W WO03054516A3 WO 2003054516 A3 WO2003054516 A3 WO 2003054516A3 US 0240991 W US0240991 W US 0240991W WO 03054516 A3 WO03054516 A3 WO 03054516A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
isolating
devices
analytes
detecting specific
Prior art date
Application number
PCT/US2002/040991
Other languages
French (fr)
Other versions
WO2003054516A2 (en
Inventor
Virginia C Gordon
Original Assignee
Safetest Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Safetest Inc filed Critical Safetest Inc
Priority to AU2002365053A priority Critical patent/AU2002365053A1/en
Priority to EP02805656A priority patent/EP1463744A4/en
Priority to JP2003555180A priority patent/JP2005533239A/en
Priority to CA002470724A priority patent/CA2470724A1/en
Publication of WO2003054516A2 publication Critical patent/WO2003054516A2/en
Publication of WO2003054516A3 publication Critical patent/WO2003054516A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/61—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving triglycerides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/34—Purifying; Cleaning
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B01L3/50255—Multi-well filtration
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4005—Concentrating samples by transferring a selected component through a membrane
    • G01N2001/4016—Concentrating samples by transferring a selected component through a membrane being a selective membrane, e.g. dialysis or osmosis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/02—Food

Abstract

Methods and systems for determining the presence of one or more analytes in various types of matricies,such as foods, oils, biological fluids, etc. Membranes are used to remove extraneous matter (e.g., solid particles) and/or to bind specific interferents or analytes. Specific assays are provided for determining citric acid, total fat (or % fat), free fatty acids, protein content and specific nucleic acid content indicative of the presence of DNA and/or RNA of certain microbes (e.g., pathogens) or genetically altered substances (e.g., genetically engineered grain).
PCT/US2002/040991 2001-12-20 2002-12-20 Devices and methods for isolating and detecting specific substances in complex matrices WO2003054516A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU2002365053A AU2002365053A1 (en) 2001-12-20 2002-12-20 Devices and methods for isolating and detecting specific substances in complex matrices
EP02805656A EP1463744A4 (en) 2001-12-20 2002-12-20 Devices and methods for isolating and detecting specific substances in complex matrices
JP2003555180A JP2005533239A (en) 2001-12-20 2002-12-20 Apparatus and method for isolating and detecting specific substances in complex substrates
CA002470724A CA2470724A1 (en) 2001-12-20 2002-12-20 Devices and methods for isolating and detecting specific substances in complex matrices

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US34242501P 2001-12-20 2001-12-20
US60/342,425 2001-12-20

Publications (2)

Publication Number Publication Date
WO2003054516A2 WO2003054516A2 (en) 2003-07-03
WO2003054516A3 true WO2003054516A3 (en) 2004-02-12

Family

ID=23341767

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/040991 WO2003054516A2 (en) 2001-12-20 2002-12-20 Devices and methods for isolating and detecting specific substances in complex matrices

Country Status (5)

Country Link
EP (1) EP1463744A4 (en)
JP (1) JP2005533239A (en)
AU (1) AU2002365053A1 (en)
CA (1) CA2470724A1 (en)
WO (1) WO2003054516A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006153488A (en) * 2004-11-25 2006-06-15 Canon Chemicals Inc Optical measurement method
WO2007137257A2 (en) 2006-05-22 2007-11-29 3M Innovative Properties Company System and method for preparing samples
FR3109585A1 (en) 2020-04-28 2021-10-29 Withings Test wafer and automated biological test system
FR3126046A1 (en) 2021-08-04 2023-02-10 Withings Improved bioassay support

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5591580A (en) * 1994-03-31 1997-01-07 Johnson & Johnson Clinical Diagnostics, Inc. Method, test element and test kit for semi-quantitative detection of target nucleic acid
US5958714A (en) * 1996-10-02 1999-09-28 Safety Associates, Inc. Test kits for determining at least two specific analytes in foods and other complex matrices
US6589779B1 (en) * 1999-07-16 2003-07-08 Board Of Regents, The University Of Texas System General signaling protocol for chemical receptors in immobilized matrices

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2649411B1 (en) * 1989-07-07 1996-09-20 Biosem QUANTITATIVE DETERMINATION AND IDENTIFICATION OF BACTERIA
US5491068A (en) * 1991-02-14 1996-02-13 Vicam, L.P. Assay method for detecting the presence of bacteria
US5438128A (en) * 1992-02-07 1995-08-01 Millipore Corporation Method for rapid purifiction of nucleic acids using layered ion-exchange membranes
JP3299330B2 (en) * 1993-03-18 2002-07-08 持田製薬株式会社 Simple measuring device and method
JPH10290691A (en) * 1997-02-19 1998-11-04 Dainippon Printing Co Ltd Gene amplification and apparatus therefor
CA2305613A1 (en) * 1997-10-22 1999-04-29 Safety Associates, Inc. Methods and apparatus for determining analytes in various matrices
US6869532B2 (en) * 2001-06-04 2005-03-22 Cuno Incorporated Nucleic acid binding matrix

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5591580A (en) * 1994-03-31 1997-01-07 Johnson & Johnson Clinical Diagnostics, Inc. Method, test element and test kit for semi-quantitative detection of target nucleic acid
US5958714A (en) * 1996-10-02 1999-09-28 Safety Associates, Inc. Test kits for determining at least two specific analytes in foods and other complex matrices
US6589779B1 (en) * 1999-07-16 2003-07-08 Board Of Regents, The University Of Texas System General signaling protocol for chemical receptors in immobilized matrices

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP1463744A4 *

Also Published As

Publication number Publication date
CA2470724A1 (en) 2003-07-03
AU2002365053A1 (en) 2003-07-09
EP1463744A2 (en) 2004-10-06
EP1463744A4 (en) 2005-10-26
WO2003054516A2 (en) 2003-07-03
JP2005533239A (en) 2005-11-04

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