WO2003060446A1

WO2003060446A1 - Apparatus and method for imaging

Info

Publication number: WO2003060446A1
Application number: PCT/US2003/001002
Authority: WO
Inventors: Robert A. Lieberman; William Rassman; Lothar U. Kempen; David Ralin
Original assignee: Maven Technologies, Llc
Priority date: 2002-01-12
Filing date: 2003-01-10
Publication date: 2003-07-24
Also published as: AU2003202980A1; IL162965A; EP1470401A4; EP1470401A1; JP2005515424A; WO2003060446A9

Abstract

Apparatus for acquiring an image of a specimen comprising a cassette (300, 302a, 302b) having an optical portion (312) holding a specimen array on a TIR surface (314) and being removably matable to a processing portion having a polarized light beam source and a processing polarization sensitive portion to image the spatially distributed charges in polarization of the specimen array. In one form the array optical portion comprises a transparent slide (312) having a bottom surface with first and second gratings located to direct polarized light to the TIR surface (314) and to direct light reflected by that (TIR) surface to an imager, respectively. The apparatus may include a flow cell (316) integral with the optical portion (312) as well as means for selecting the direction and wavelength of the polarized light.

Description

UTILITY PATENT APPLICATION

TITLE: APPARATUS AND METHOD FOR IMAGING

INVENTORS: ROBERT A. LIEBERMAN, WILLIAM RASSMAN, LOTHAR U. KEMPEN AND DAVID RALIN

REFERENCE TO RELATED APPLICATIONS

This application is a continuation-in-part of application serial number 09/838,700 filed on April 19, 2001 , and is a continuation-in-part of application serial number 09/614,503 filed on My 11, 2000, the Content of which are incorporated herein by reference.

FIELD OF THE INVENTION This invention relates to imaging techniques and apparatus in conjunction with total internal reflection at the boundary of an optically transparent material and more particularly to the use of such techniques and apparatus for detecting the presence, composition, quantity, and spatial distribution of substances on optically transparent substrates.

SUMMARY OF THE INVENTION

This invention relates to imaging of a biochip (also referred to as a gene chip, protein chip, microarray and others) useful in applications, which, for example, utilize local polarization changes detected in the respective parts of the emerging light beam. U.S. Pat. No. 5,633,724 to King, et al. (1997) describes the readout of a biochemical array using the evanescent field. The King et al patent focuses on fluorescent assays, using the evanescent field to excite fluorescent markers attached to the substances to be detected and analyzed. The attachment of fluorescent markers or other molecular tags to the substances to be detected on the surface requires an additional step in performing the measurement, which is not required in the current invention. The King et al patent further describes use of a resonant cavity to provide an evanescent field for exciting analytes.

The formation of an array of biologically or chemically active spots on the surface of a substrate for identifying constituents in test material brought into contact with the array is well known. Typically, such processes require spots of, for example, oligonucleotides, DNA clones, antibodies, peptides, receptors, enzymes, inhibitors, etc. which are processed to exhibit fluorescence, electroluminescence, current change, voltage change - - etc. for providing a detectable signature for the presence of constituents in the material being tested. In accordance with the principles of this invention, light from a light source member providing an extended, polarized light beam, is directed through a transparent substrate and undergoes total internal reflection (TIR) at the surface ofthe substrate by a single reflection within the TIR member. Total Internal Reflection is described in: M. Born, and E. Wolf, "Principles of Optics", 6^th ed., pp 47-51, Pergamon Press, Oxford, 1991. The reflected light is detected by a polarization-sensitive, two-dimensional array detector or other type of detector. The changes ofthe local polarization state in the beam's cross-section caused by the total internal reflection are employed to obtain information about the presence and composition of substances in an array of substances on the substrate surface for each point ofthe surface. In accordance with one aspect of the invention, the light-generating element within the light source member is a quasi- monochromatic light source of moderate bandwidth.

In one embodiment, the light-generating element within the light source member is an Arc lamp. The light from the light source member is directed through an internal reflection member to reflect off a specimen. The total internal reflection at any point within the cross-section of the light beam causes a phase shift between the light component polarized in the plane of incidence and the component polarized perpendicular to the plane of incidence. The reflected light is detected by a polarization- sensitive detector such as a two dimensional array detector and the signal from this detector is then processed in a computer to provide two-dimensional information about substances on the surface of the specimen. Spatially distributed changes in polarization state in the cross-section of the reflected beam are indicative of he substances in the specimen in the location in the specimen array corresponding to a position in the detector. The apparatus and method is especially adapted for imaging material in an aqueous solution. It is furthermore particularly suited for detecting attachment and detachment of analytes to a two-dimensional biomolecular array positioned on the total internal reflection member as part of a molecular thin film system. In various applications a plurality of discrete specimen spots are presented in an array, where the method and apparatus will image the array so as to distinguish each ofthe discrete specimen spots. Fluorescence or molecular tagging is not necessary or practical for use in this invention. Further, in accordance with the principles of this invention, the apparatus disclosed in the above-identified parent application provides an image of an entire array on a biochip or if desired a portion ofthe entire array.

In further embodiments the invention relates to a device having a removable portion, which in preferred embodiments is disposable or reusable, the removable portion defining a cassette having an optical element capable of receiving polarized light and directing it to a TIR surface to create an evanescent field in which the material to be imaged is positioned, and then upon a single reflection, reflecting the light beam to exit the optical element; the cassette also having a mounting structure to enable it to be mounted in the processing portion ofthe device that produces the polarized beam and receives it after reflection, for analysis. The cassette is designed to mount on the processing portion for operative interaction with the light beam as described herein. The cassette may be provided with a microarray of spots already in place on the optical element or it may be configured to receive a separate slide on which a microarray of spots have been placed. The cassette may be either disposable or reusable. When a separate slide is used in conjunction with the optical element, it is mounted on the optical element with an index matching fluid so that the surface of the slide on which the microarray is placed is the TIR surface.

In one aspect of the invention the optical element is a prism. A further specific aspect of the invention comprises the use of an optical element having gratings on it to control the direction of the incoming beam to enable TIR to occur instead of using a prism. One ofthe goals is to define a cassette, which is sufficiently inexpensive- that it is practical to dispose of it after a single use. While prisms can be used and reused, they are expensive. The aspect of the invention that uses gratings provides for a much less expensive cassette. Nevertheless the use of gratings on an optical element to enable TIR to occur may be implemented broadly for any apparatus or method as an alternative to use of a prism. Also, certain other techniques are made available by use of gratings such as electronically alterable gratings and the use of holographic gratings.

BRIEF DESCRIPTION OF THE DRAWINGS

Fig. 1 is a block diagram of an illustrative system in accordance with the principles of this invention;

Fig. 2 is a block diagram of an embodiment ofthe system of fig. 1;

Figs. 3, 4 and 5 are block diagrams of alternative portions ofthe system of fig. 1; Fig. 6 is an exploded view of a combined optical assembly and a flow cell;

Fig. 7 is a schematic side view of an alternative optical element;

Fig. 8 is a schematic block diagram of an alternative light source arrangement;

Fig. 9 is a schematic block diagram of an alternative optical assembly;

Fig. 10a is a schematic side view of a specimen cassette ready for positioning in the processing assembly;

Fig. 10b is a schematic side view of a specimen cassette positioned in the processing assembly; and

Figs. 11a, l ib, l ie, and 1 Id are schematic views of a slide and slide holder for an embodiment o the system.

DETAILED DESCRIPTION

The invention comprises a method and apparatus for analyzing a two-dimensional arrangement of chemical substances with an imaging technique. A polarized light source of known polarization state is directed into a total internal reflection member (TIR member) configured for a single reflection at a total internal reflection surface (TIR surface) and then exiting the TIR member. In the context of this document, superposition of reflections as encountered at a layered optical structure where the layer thicknesses are smaller than the coherence length of the illuminating light is referred to as a single reflection. The chemical specimen is in place above the TIR surface in the evanescent field of the reflected light beam. After reflection, the beam is passed to a polarization- sensitive two-dimensional detector such as a polarizer and a camera or other types of detectors. The beam's content can then be processed to determine the change in polarization state, locally in the two-dimensional cross-section ofthe beam. This provides a spatially distributed map of change of polarization state in the specimen. A variety of techniques are available to determine the change in polarization such as measuring the deviation from a null condition or by comparing the input polarization state to the output polarization state.

The refractive index composition ofthe materials within the evanescent field determines the change in the polarization state ofthe beam due to the reflection at the TIR surface. A two-dimensional variation of this composition within the TIR surface is associated with a respective variation of the polarization state spatially distributed across the cross-section ofthe reflected light beam.

In one application, the chemical specimen forms a two-dimensional array of molecules (here referred to as receptors) with specific affinities towards respective other molecules (here referred to as ligands). In this application, the invention is utilized to indicate the presence or absence or rate of binding between ligands and receptors on the array. Such arrays commonly consist of a plurality of discrete specimen spots. The present method and apparatus images the array so as to distinguish each of the discrete specimen spots represented by the local change in polarization state in the cross-section of the reflected beam. Subject to limitations in resolving power of the detector, the invention permits measurement of thickness and/or refractive index composition of the specimen under investigation with a very high resolution, in the sub angstrom range, spatially resolved over an entire area. The invention is particularly useful in applications where the specimen is in an aqueous solution. In a particular application, the present invention is used to determine the presence of biological agents in a solution such as in immunosensor applications by measuring their attachment to antibodies on the TIR surface in the evanescent field. In another application, the present invention is used to determine the presence and structure of nucleic acid sequences in a solution by measuring their attachment to other nucleic acid sequences on the TIR surface in the evanescent field. Described in more detail below are different embodiments ofthe invention. Figs. 1 and 2 show an apparatus, which implements one embodiment ofthe invention. As shown in Fig. 1, the apparatus 10 can be described conveniently as consisting of three general portions. Portion 12 is a polarized light source assembly, portion 14 is a total internal reflection assembly providing a single reflection and portion 16 is a polarization-sensitive imaging detector assembly which can employ for example a two-dimensional array detector. Data from the detector assembly 1 is sent by an electrical signal 24 to processor 18 such as a specially programmed computer and user access system such as a print-out or image display. Data can be presented as an image, a data table, graph or in other forms. The polarized light source assembly 12 passes polarized light of known polarization state, (which may be varied or varying) 20 to the total internal reflection assembly 14 where a single reflection occurs and the reflected light 22, having a changed polarization state passes to the detector assembly 16, where it is recorded spatially over the cross-section ofthe beam. The recorded data are sent to the processor 18 where the change of polarization state, is determined to provide a spatially resolved map of changes in polarization state. Where the specimens are presented as an array of discrete spots, each spot will be imaged for its change in polarization state within the spot area.

Fig. 2 shows a more detailed schematic block diagram ofthe preferred embodiment. The polarized light source assembly 12 has a light source 26, a beam forming member 28 (if the nature ofthe light source is such as to make beam forming useful or necessary) a polarizer 30 and an optical retarder 32. The total internal light reflection assembly 14 has an optical element 34 which has an optical surface 36. Also shown is a specimen slide 38 on the optical surface 36, and between them an index matching substance 40. Because substance 40 is index matched a total internal reflection surface (TIR surface) is defined as the upper surface 39 of the specimen slide 38. A specimen 42 is positioned on the total internal reflection surface 39 ofthe slide 38. In one embodiment o the invention, the optical element 34 is a prism configured along with the index-matched slide 38 in relationship to the incoming light beam 20, and the exiting light beam 22 such that the beam reflects only a single time at the TIR surface 39 and then exits the prism. If the specimen is placed directly on the optical surface 36, then the optical surface 36 would be the TIR surface. But this is not the usual application, since the specimen (such as a biochip) is usually prepared more conveniently on a specimen slide 38 and placed in the apparatus. However constructed, the invention incorporates an optical structure having a TIR surface and the beam reflects only a single time at the TIR surface between entering and leaving the optical structure. In other words, there is a TIR surface in optical contact with the specimen, such that the evanescent field associated with the total internal reflection interacts with the specimen, and there is only a single reflection at that TIR surface.

The post reflection detector assembly 16 has a polarizer 44, and an imaging detector, for example a two-dimensional array detector 46, preferably a camera ofthe CCD or CMOS array type. The processor 18 is a specially programmed computer (or processor) and output means for processing the imagery into a representation of film thickness variations spatially resolved over the cross-section ofthe area imaged. The imaging is acquired by detecting changes spatially distributed in the local polarization state in the beam's cross-section caused by the total internal reflection. This provides information about the presence and composition in the array of substances on the substrate surface for each resolvable point on the surface. Different polarization state changes are included in the cross-section ofthe reflected beam indicative ofthe substances on the specimen in the location in the specimen array corresponding to a position in the detector.

The processor 18 receives the data as an electrical signal (on connector 24) and characterizes the change of polarization state spatially over the two-dimensional array. In the processor 18, the analysis and processing is done in one embodiment by comparing the known polarization state of the incoming light from the light processing assembly 12 with the changed polarization state of the reflected light 22, spatially resolved two- dimensionally within the beam which provides a map of spatially distributed points or spots in the specimen array. The polarization shift is then analyzed by the processor 18 to provide information o the presence and properties of elements in the chemical specimen. Other known techniques, such as null processing can be used to determine the change in polarization state.

Alternatively, the light source member 26 may be an LED, an SLD (Super Luminescent Diode), an incandescent light source, or a laser. If an LED or SLD is used, the set-up shown in Fig. 2 is appropriate, where the beam-forming member 28 is a collimator. If an incandescent light source is used, an optical filter is also used. In one embodiment, the light source 26 for the apparatus is a quasi-monochromatic light source of moderate bandwidth. In accordance with the invention the light source 26 is preferably an LED of moderate bandwidth. Preferably the bandwidth is a full width half maximum wavelength in the range of about 10nm-50nm, and more preferably a full width half maximum wavelength in the range of about 30nm-50nm.

In an alternative embodiment, the optical retarder 32 as shown in Fig. 2, could be placed instead in the exiting beam path 16 at the location 22 before the polarizer 44.

Fig. 3 shows an additional embodiment. In embodiments in which the light source is a laser 50, a moving diffuser 52 is adapted to produce speckle-offsetting fluctuation of the minima and maxima in the speckle pattern caused by the laser. The moving diffuser 52 is attached to a mechanical actuator 54 which is preferably a motor and servo- apparatus for providing the speckle offsetting fluctuations. The beam 20 then proceeds through the beam-forming element 28, the polarizer 30 and the optical retarder 32, exiting the light source assembly 12 at the location 20.

The polarizer 30, in embodiments as shown in Figs 2 and 3, employs a polarizer of selected known polarization state. The polarizer 30 may be of the type having a mechanical actuator driven by a motor control signal so as to enable the variation and selection ofthe polarization state ofthe light beam 20. As mentioned above, the total internal reflection optical element 34 (of fig. 2) either alone or in combination with an index matched slide may be arranged for use with a specimen in various ways to define a total internal reflection assembly so long as the specimen is in the evanescent field of he reflected beam 20,22.

As noted above, the specimen 42 (of fig. 2) could be set directly on the optical surface 36 in which case the optical surface 36 would be the TIR surface. But this is inconvenient and repeated use is likely to degrade the optical quality of the optical surface 36. Therefore, consistent with common practice in which a biochip or other chemical assay specimen is provided, a specimen slide 38 or other supporting apparatus is employed. It is common in a biochip to provide an array of discrete specimen spots supported on a structure for obtaining analysis of each spot. The term total internal reflection optical element refers to lαiown optical elements alone or in combination with other elements which provide the phenomenon known as total internal reflection. Fig. 2 shows use of a prism combined with a slide 38, being index matched so that there is a TIR surface 39.

Fig. 4 shows an alternative optical arrangement in which a flat optical member 56 having an upper surface 58 is surmounted by a specimen slide 60 with an index matching substance 62 on which is a specimen 64 is mounted. The TIR surface 66 is the top ofthe slide 60. The beam 20 enters the assembly, is refracted as it enters, and leaves the optical member 56 after a single reflection at the TIR surface 66 as beam 22. Other mechanisms for providing total internal reflection and an evanescent field can be employed in practicing this invention as long as only a single reflection occurs at the TIR surface upon which the specimen is placed so as to be in the evanescent field associated with the reflection. As seen in Fig. 5, the post-reflection processing arrangement 16 through which the beam 22 passes, can alternatively, consist of a polarizer member 70, a beam forming member 72 and an imaging detector 74 such as a two dimensional array detector or other type of imaging detector.

The method and apparatus can be used in combination with biochips ofthe type having discrete specimen spots or a micro-titer plate containing an array of discrete spots or locations for analysis, where the detected change in polarization state is spatially related to the discrete locations in the reflected beam. Therefore, as used herein the slide and specimen refers to any type of chemical or biological array that is desired to be examined.

The foregoing described apparatus and methods are especially beneficial for imaging materials in an aqueous medium.

The invention as described above provides an extremely sensitive optical imaging system for real-time imaging of the binding status of biochip array elements on the surface of an optically transparent material such as a glass or plastic chip. An exemplary monitored array a 15mm square inscribed in the 20mm circular field, with discrete specimen spots of size commensurate with the lateral resolution of the imaging optics resulting in fully parallel, continuous real-time readout of up to 5 million sensor fields. Sensor sensitivity to surface attachment is in the femtogram/mm² range (e.g. one DNA per square micron).

The apparatus of Fig. 1 operates by imaging the pattern of reactions on the biochip. Those reactions produce changes in the height, surface concentration, or refractive index ofthe material that reacts at each spot. The area imaged could be the entire biochip array or a portion ofthe entire biochip array. By providing an array of spots of different materials, different constituents in test material flowed over the spots bind in a manner which identifies those constituents. By including in a computer memory the positions of the various materials in the different spots ofthe array, the image produced by the apparatus of Fig. 1 not only identifies the constituents in the test material, but also can determine the rate at which the reactions occur. With the apparatus described, height differences can be imaged dynamically over such short periods of time that intermediate height change readings can be recorded and therefore height change rates can be determined as well as allowing comparison ofthe rate of height change or intermediate amount of height change among the spots on the biochip array. A process using the apparatus and a biochip is as follows: Place the biochip surface in a flow cell in combination with the apparatus such that the surface ofthe slide which has the spots is the TIR surface; initially calibrate the apparatus, such that light reflected from the biochip yields a fully-linear polarization; and adjust the analyzer to a null position to fully block the linearly polarized light.

When the null position is achieved, every region where the chip (slide) surface deviates from the initial state stands out as a bright spot the intensity of which is directly related to the thickness (height) differences induced by the deviation. A solution containing target molecules are flowed over the biochip surface. The intensity changes caused by changes in the polarization of the light reflected by the biochip may be monitored continuously across the array to study thickness changes that occur on the biochip. The relative intensity measured at the detector is related to the sample parameters and the setting ofthe polarizing elements using computer programs based on a detailed "Jones calculus" sensor system description. With these programs, theoretical plots are fitted to the acquired measurement data sets, and the outputs' dependence on parameter variations can be visualized. At the start ofthe procedure, the apparatus is adjusted so that the image of the entire biochip is homogeneously dark. As the target molecules begin to bind to the reference probes the intensity of the images of the spots increases. The intensity is expected to be the highest for high affinity interactions and mild changes are expected for low affinity interactions. The rate of intensity change can be related to the affinity constraints ofthe system. For example, using a properly prepared biochip, in a single procedure, the affinity measurements for multiple peptides can be performed. Moreover, the effect of binding density (because different concentrations of peptides are used in spotting) can also be measured.

Although reference is made to biochips in the example, the procedure and the results apply generally to chemically sensitive materials on a TIR surface.

The invention has been described above in terms of a prism having a TIR surface, and having on the surface an array of molecular spots. In another embodiment, also described above the array is formed on a separate slide positioned on the prism with index matching fluid between them so that only the upper surface ofthe slide forms the TIR surface.

In another aspect ofthe invention referred to as the cassette embodiment, the . invention is configured such that the portion on which the micro array spots are placed (also called the specimen array) is a partially or wholly disposable cassette or a reusable cassette.

In embodiments where the cassette is a separate or separable structure, the assembly elements, which include the light source and its related elements and the elements that receive, record and analyze the light after it has been reflected in the specimen cassette, is in aggregate defined herein as the processing assembly.

A basic exemplary separate cassette of this type comprises an optical assembly which may be simply an optical element that produces the evanescent field and the single TIR including, as an integral part, the surface that contains the microarray (the specimen). The cassette also includes a mount or frame portion in which the optical assembly is held. The mount or frame portion is configured along with mating configuration in the processing assembly to fit operatively in the processing assembly. This cassette assembly can be manufactured and provided to users who have available the other portion ofthe device, the processing assembly, that being the portion that provides the light source and light processing elements before the polarized light beam enters the optical assembly ofthe cassette as well as the portions that process the light after it exits the optical assembly ofthe cassette. The two assemblies, the cassette and the processing assembly are constructed so that the cassette is fitable to the processing assembly in such a way that they function cooperatively as described above. Also, the cassette can be constructed to include or receive a flow cell either manufactured as an integral part ofthe cassette or configured to mate to the cassette optionally, as desired. The cassette can be made to be reusable or disposable while the processing assembly comprises a permanent device available to users who will obtain and use the cassettes as needed.

Such an embodiment ofthe invention is directed at providing a wholly or partially disposable or reusable cassette in which the optical element and the mount or frame portion define a cassette. The cassette in its most basic configuration has an optical element or optical assembly that has a surface on which a specimen array has been placed, that surface defining the TIR surface. The optical element or optical assembly also has an optical transmission portion, which directs the extended beam of polarized light from the processing assembly to the TIR surface to create an evanescent field by a single reflection ofthe beam, and then directs the beam out ofthe optical transmission portion back into the processing assembly. The frame or mount portion allows the optical element or optical assembly to be carried safely and to be easily and accurately installed for use in the processing assembly.

Alternatively, the specimen array can be provided on a separate slide placed on the optical element, preferably with index matching fluid, so that the upper surface of the slide will be the TIR surface. The other assembly, the processing assembly has a receiving and locating portion that is matingly configured with the configuration of the frame or mount portion o the specimen cassette such that the cassette will fit in sufficiently precise orientation in or on the processing assembly. Orientation ofthe cassette with the processing assembly is important in order to ensure that the polarized light from the processing assembly will be properly directed to reflect a single time at the TIR surface and produce an evanescent field encompassing a single one, a sequence of areas, or all of the desired areas of the specimen array on the TIR surface. In other words, the cassette is oriented such that evanescent effects occur on the TIR surface upon which the desired specimens lie. The reflected light then exits the specimen cassette to be received by a reflected light-analyzing portion ofthe processing assembly. The light analyzing portion determines the change in polarization spatially in the evanescent field to give a two dimensional image.

In a configuration ofthe processing assembly in which the polarized light source section and the reflected light analyzing section are not adjustable, more precise orientation ofthe cassette with the processing assembly is required in order that the incoming beam be directed as desired at the TIR surface and the outgoing beam be redirected to the analyzing section. In particular, the specimen array is placed on the TIR surface in the evanescent field. However, in a configuration in which either or both of the light source section and the analyzing section are adjustable, a number of advantages are available. One advantage is that orientation ofthe cassette need only be sufficiently precise to be within the range of adjustability of those adjustable sections. Other advantages of adjustability address the variable size and arrangement ofthe specimen array or sub-arrays on the TIR surface, the adjustability allowing proper direction and focusing ofthe light beam where the specimen array(s) are in different locations or arrays cover different areas or for selection of sub-arrays to be analyzed separately.

The adjustability allows proper direction and focusing ofthe light beam for single reflection and placement of the evanescent field where varying size and arrangement of specimen arrays and sub-arrays are in different locations, where arrays cover different areas and where selection of imaging sub-arrays is desired. In addition to or as an alternative to making the processing assembly adjustable, this adjustability can be build into the cassette, so as to place a desired portion ofthe specimen array or sub-arrays within the path of the light beam for a single TIR at the TIR surface.

An exemplary configuration of a cassette is shown in Fig. 6 in which a prism 300 is held in prism mounts 302a and 302b by way ofthe retaining surfaces 304a and 304b formed into the inside faces of the prism mounts 302a and 302b respectively (not shown). Therefore, when the prism mounts 302a and 302b are secured together as with screws 306 with the prism 300 between them, the prism is locked in place. In this configuration, the prism 300 and the prism mounts 302a and 302b define a basic cassette when the specimen-array spots are placed on the surface 308 which is the top surface ofthe prism, and operates as the TIR surface. The prism mounts 302a and 302b are configured to be received in the processing assembly in the proper orientation for use. In this configuration as least the prism 308 is disposable or reusable after using the microarray of spots that have been positioned on its top surface 308. Alternatively, a sample slide 312 can be used as a carrier for the micro array spots by being placed on top ofthe prism surface 308 with an index matching fluid between them so that the top surface 314 is the TIR surface. In this configuration only the slide 312 would have to be disposable.

Subject to cost considerations, the entire cassette could be disposable. In any case the cassette can be reusable with a new biochip or even a reclaimed biochip.

Alternatively, when desired the cassette can be defined by the addition as shown in Fig. 6 of a flow cell 316 along with a gasket 318. In a further embodiment, the slide itself can provide a TIR surface in the absence of a prism as described hereinbefore. In effect, the slide can serve the purpose ofthe prism, which is directing the light to and from the TIR surface at a proper angle to cause ^'total internal reflection as well as providing the TIR surface. But in this embodiment, the bottom surface ofthe slide includes a grating operative on light entering the slide at an appropriate angle to create the requisite TIR conditions for the evanescent field above the TIR surface, with an array of localized polarization changes due to the change to specimen array spots, to be imaged in a single reflection at the TIR surface.

Fig. 7 shows schematically a slide 410 with gratings 412a and 412b formed on its bottom surface (as viewed). The gratings are placed so as to intercept the incoming light beam 414a and outgoing light beam 414b respectively, to provide the correct angle for a single TIR at the surface 416 on which a specimen array 418 is placed. It is clear that a slide with a grating on its bottom surface (as viewed) serves the purpose of a prism (directing the light at the correct angle) as well as providing an inexpensive and disposable slide on which specimen array may be formed for imaging. With the optical element therefore being defined as a slide with appropriate gratings as described to achieve a single TIR a very inexpensive fully disposable cassette can be configured. It is to be understood that gratings are operative to direct polarized light in a manner consistent with the TIR, single reflection conditions to receive the reflected light in a polarization sensitive detector so that by resolving changes in polarization spatially over a two dimensional area, the area can be imaged in accordance with the principles of this invention.

However the use of gratings as described, to divert light from total internal reflection, is not limited to the single reflection concept as described above, but can be employed in any case which a TRI surface and an evanescent field are desired. These include for example applications where a plurality of reflection occur and in cases using surface plasmon resonance structures

Gratings may be either ofthe surface- relief type or ofthe refractive index type, and may be fixed or electrically controllable. Fixed surface-relief gratings, and methods for their fabrication, are well understood, and are described in Born and Wolf (op cit.), pp401-418. Electrically controllable surface- relief gratings include surface acoustic wave (SAW) devices. Refractive index gratings, sometimes referred to as Bragg gratings, consist of periodic variations in refractive index, and may be either fixed or, when fabricated in electooptically active materials, electrically controllable. Acousto- optic effects can also be used to create electrically tunable refractive index gratings (Handbook of Optics, op cit., Vol. 2, Chapter 12). Bragg refractive index gratings and methods for their fabrication in optical fibers are discussed in G. Meltz, et al. Optics

Lett., 14, p823, 1989. Fabrication of Bragg gratings in other glass materials, such as slide 410 in Fig. 13 or optical member 600 in Fig. 15 can be accomplished using similar means.

An electrically alterable grating allows for the control ofthe depth of penetration ofthe evanescent field into the specimen array. That is, the height of the evanescent field above the TIR surface can be controlled. This control over the depth of penetration also can be accomplished by a mechanism to permit the movement of the light source along an arcuate path to change the direction o the light input or by the use of a filter at the light source to change the wavelength of the input light. Control over the depth of penetration of the evanescent field permits the acquisition of real time data representative o the changes in height and/or mass of molecular bindings at the various sites of the specimen array by successive output images with varying depth of penetration of the evanescent field.

In embodiments, in accordance with the principles of this invention, it may be desired to employ light of differing wavelengths for examining receptor ligand interactions. Techniques for permitting control over the use of differing wavelengths include the use of a filter wheel and/or the changing ofthe angle at which light is directed from the light source. The use of a dielectric stack filter with collimated light also permits the elimination of light at all wavelengths except a selected wavelength. The inclusion of more than a single imager also is useful in embodiments employing such techniques. These techniques also allow for wavelength selection choices, which avoid any noise, which may be present in the system.

Fig. 8 schematically illustrates an embodiment in which a polarized light source 512 is mounted on a mechanism (not shown) for repositioning the light source along an arcuate path indicated by curve 514. Further, the light from source 512 is directed through a filter wheel 516. Filter wheel 516 and the position of the light source along curve 514 are controlled by controller 518 for selecting the depth of penetration ofthe evanescent field (i.e. its height over the TIR surface). The above depth control techniques may be used separately or in concert.

Fig. 9 also shows an optical member 600 for a use in a cassette having a lower surface 602 on which gratings 604 and 606 have been placed. The TIR surface 608 contains a microarray 610. Also, shown are grating biasing field sources 612 and 614 for altering the spacing in gratings 604 and 606 if electrically alterable gratings are formed on the slide surface 602. The figure also shows a plurality of imagers 616, 618, such as CCD memories; such imagers are located in different positions for capturing (input) light directed at (1) different angles along curve 514 of Fig. 8 or (2) at different angles imposed by the gratings. The output of any of the images are applied to a computer 620 for analysis. Therefore polarized light 624 enters the optical member 600 passing through the gratings 604 and then, after reflection at the TIR surface 608, light 626 passes through the gratings 606. Figs. 10a and 10b show a representative cassette 700 in which the optical element has gratings for directing the light beam. Specifically, Fig. I0a shows an optical element 710 having a lower surface 714 on which gratings 716a and 716b have been placed. It has an upper surface 718 on which the specimen array 720 resides. The cassette 700 has a frame or mounting portion 724 and a retaining collar 726 to receive and retain the optical element 710 in the cassette 700. The frame or mounting portion 724 has pins 728. Also shown is a portion ofthe processing assembly 730 having receptacles 732 for receiving the pins 728, when the cassette 700 is lowered into place as indicated by arrows A- A. Thus, as seen in Fig. 16b when the cassette is in place, the light beam 734a and 734b can enter and exit respectively the optical element 710 properly for TIR at the surface 718. Clamps 736 hold the cassette 700 in place in the processing assembly 730. Another exemplary configuration is cassette 800 using a slide as shown in Figs.

11a through l id. That configuration comprises a frame 802 and an optical member 804 on which a specimen array 806 is applied as shown in Fig. l id. The frame 802 has a first side 808 and a second side 810, each of which has a mating connection portion 812a and 812b in this case on opposite legs ofthe sides 808 and 810 respectively. In this configuration the connection portions 812a and 812b comprise an aperture 814 on one leg and a detent 816 on the other leg. The frames sides 808 and 810 have recessed shelves

819 and 820 that match when the two parts are assembled. The recessed shelves 819 and

820 are sized to retain the optical member slide 804 that is configured to fit into the shelves. The frame has a minimum of two posts 822 extending downwardly, four posts being preferred. The posts fit into mating receptacles in the processing assembly for locating the specimen assembly with the processing assembly. This cassette 800 can be used in conjunction with a prism, or as an independent slide, or the lower surface of the slide 804 can be fitted with gratings as described above. Certainly numerous configurations can be devised to construct a cassette having an optical member, a TIR surface on which a specimen array is placed and a mount, the cassette being configured to fit in or on the processing assembly to function as described herein.

An important process available with the devices described above is dynamic imaging, that is in addition to recording an image at the end of a procedure such as a binding experiment run with a flow cell over a microarray, images can be captured and processed intermittently between the start and the end so that intermediate status and time progression of the binding experiment can be analyzed. What has been described herein is merely illustrative ofthe principles of this invention. It is well within the purview of those skilled in the art to design various, modifications ofthe invention within the spirit and scope ofthe following claims. For example, it may be advantageous to image a single portion of an array of interactions on a slide or to scan a succession of such portions. Further, it may be advantageous to aperture the image directed at the imager to obtain scattering cross sections. Such expedients relate to applications ofthe apparatus herein.

Claims

WHAT IS CLAIMED IS:

1. Imaging apparatus comprising an optical assembly, a light source assembly and an imaging detector, said assemblies being operative to provide an image at a TIR surface defined by said optical assembly, said light source assembly directing polarized light at said TIR surface in a manner to generate an evanescent field at said surface, said optical assembly having spaced-apart, top and bottom surfaces, said bottom surface including first and second gratings, said gratings being located and configured to direct light passing through the first grating from said source to said top surface and light reflected from said top surface passing through to said second grating to said detector respectively to cause TIR and an evanescent field associated therewith by a single reflection at the TIR surface.

2. Apparatus as in claim 1 wherein said first and second gratings comprise Bragg gratings.

3. Apparatus as in claim 1 wherein said gratings comprise electrically alterable gratings, said apparatus also including means for altering said gratings in a manner to change the direction of light incident thereto.

4. Apparatus as in claim 3 wherein said gratings comprise electrically alterable holograms.

5. Apparatus as in claim 1 also including a wavelength filter for selecting the wavelength of light from said light source.

6. Apparatus as in claim 1 also including an arcuate adjusting mechanism for moving said light source about an arcuate path to selected positions therealong to alter the angle of the light into the optical element.

7. Apparatus as in claim 1 including a flow cell secured to said TIR surface, said flow cell including an inlet port and an outlet port for flowing analyte across said TIR surface.

8. Apparatus as in claim 1 also including a computer, said computer including means for converting images from said reflected light into a convenient form for analysis.

9. Apparatus as in claim 5 wherein said filter means comprises a filter wheel located to intercept light from said light source, said apparatus including means for rotating said wheel.

10. Apparatus as in claim 5 wherein said filter means comprises a stack filter.

11. Apparatus as in claim 3 also including a plurality of imagers, each of said imagers being located at a position to capture an image generated in response to input light at a corresponding angle.

12. Apparatus as in claim 5 also including a plurality of imagers each of said imagers being located at a position to capture an image generated in response to input light of a corresponding wavelength.

13. Apparatus as in claim 7 wherein said flow cell includes means for securing a slide thereto.

14. A cassette for use with a TIR analyzing instrument, said instrument comprising a processing assembly having a polarized light source portion providing a polarized light beam, a reflected light analyzing portion and a locating portion for locating the cassette for TIR imaging of a specimen array on an optical element of said cassette comprising; an optical element comprising an upper TIR surface on which a specimen array is formed, said optical element being configured to receive polarized light directed to the TIR surface and generating an evanescent field at the TIR surface; a mount or frame coupled to the optical element and having a locating portion engageable with a mating locating element of the TIR instrument said mount or frame being operable to locate at least a selected portion of the specimen array in the evanescent field.

15. Imaging apparatus as in claim 14 wherein said TIR surface comprises the upper surface of a transparent slide said slide having a lower surface, said lower surface comprising at least one grating and said upper surface including an array of receptors thereon.

16. Apparatus as in claim 14 wherein said at least one grating comprises a first grating positioned to intercept said polarized light beam from said light source portion and a second grating positioned to intercept said light beam after it has reflected from said upper TIR surface.

17. Apparatus as in claim 14 wherein said cassette and said processing assembly are configured for a single reflection of the light beam at the TIR surface.

18. Apparatus as in claim 14 further including a flow cell secured to said TIR surface, said flow cell including an inlet port and an outlet port for flowing analyte across said specimen array.

19. Apparatus as in claim 15 wherein at least one grating comprises an electrically alterable grating.

20. An imaging apparatus for imaging a specimen array within the evanescent field present upon reflection of a beam of light at a TIR surface comprising; a polarized light source emitting a polarized extended beam of light; an optical element having a TIR surface on which the specimen array is placed and a surface having grating portions, the optical element being placed to cause the beam of light to pass through a first grating portion to direct the light to the TIR surface for being reflected only a single time at the TIR surface, the specimen array being within the evanescent field associated with the total internal reflection at the TIR surface and the beam of light after said single reflection passing through a second grating portion, said gratings being configured to pass the beam of light through them to the TIR surface and from the TIR surface to enable TIR effect to occur; and a polarization - sensitive, imaging detector, said detector detecting the beam of light reflected from the TIR surface including the spatially distributed polarization change caused by the specimen array.

21. The imaging apparatus of claim 20 wherein the light source and imaging detector are constructed as a first separate assembly defining a processing assembly and the optical element is part of a second separate assembly defining a cassette and the cassette is removably fitted to the processing assembly.

22. The imaging apparatus of claim 21 wherein the cassette comprises the optical element having an upper surface defining the TIR surface on which the specimen array is placed and a lower surface having the first grating portion and the second grating portion each grating portion positioned so that the beam of light from the processing assembly will pass through the first grating portion into the optical element and after reflection will pass through the second grating portion prior to exiting the optical element.

23. The imaging apparatus of claim 22 wherein the cassette has a mount or frame portion to which the optical assembly is attached and the mount or frame portion has mating portions and the processing unit has mating receiving portions such that by matingly fitting the mating portions to the mating receiving portions the cassette is removably fitted to the processing assembly.

24. The apparatus of claim 23 in which at least one of said grating portions is electronically alterable and further comprising controllable electronics altering means for electronically controlling at least one grating whereby the angle of the beam of light exiting from the at least one electrically alterable grating may be varied.

25. The apparatus of claim 23 in which the light source in the processing assembly may be changed by an arcuate adjusting mechanism to be directed at selected different angles toward the optical element of the specimen assembly to alter the angle of the light into the optical element.

■ 26. The apparatus of claim 23 wherein said and said processing assembly are movable in relation to each other to enable selecting portions of the specimen array to be sequentially imaged.

27. Apparatus of the type in which total internal reflection of a light beam occurs at a TIR surface in which an evanescent field is created, by passing a beam of light into an optical member or assembly of members defining an optical portion the improvement comprising; a first grating at a surface of the optical portion at which the light beam is directed into the optical portion the first grating being configured to direct the light beam through the optical portion to the TIR surface to cause total internal reflection at the TIR surface. a second grating at a surface ofthe optical portion the second grating being configured for the light beam to exit the optical portion through the second grating.

28. The apparatus of claim 27 in which the apparatus has material under investigation in the evanescent field and said material causes a change in polarization of the light beam.

29. The apparatus of claim 27 in which the light beam exits the optical portion after a single reflection at the TIR surface.

30. The apparatus of claim 27 in which the light beam is polarized prior to entering the optical portion.

31. The apparatus of claim 30 in which the polarized light beam has a first coherent length and the optical portion has a first distance between the first grating and the TIR surface and second distance between the TIR surface and the second grating, said first and second distances each being smaller than said first coherent length.

32. Imaging apparatus comprising an optical assembly, a light source assembly and an imaging detector, said assemblies being operative to provide an image at a TIR surface defined by a top surface of said optical assembly, said light source assembly directing at said TIR surface polarized light having a first coherent length in a manner to generate an evanescent field at said TIR surface, said optical assembly having a bottom surface spaced apart from said top surface a distance smaller than said first • coherent length said bottom surface including first and second gratings, said gratings being located and configured to direct light passing through the first grating from said source to said TIR surface and light reflected from said TIR surface passing through to said second grating to said detector respectively to cause TIR and an evanescent field associated therewith by a single reflection at the TIR surface.

33. Apparatus as in claim 32 wherein said first and second gratings comprise Bragg gratings.

34. Apparatus as in claim 32 wherein said gratings comprise electrically alterable gratings, said apparatus also including means for altering said gratings in a manner to change the direction of light incident thereto.

35. Apparatus as in claim 34 wherein said gratings comprise electrically alterable holograms.

36. Apparatus as in claim 32 also including a wavelength filter for selecting the wavelength of light from said light source.

37. Apparatus as in claim 32 also including an arcuate adjusting mechanism for moving said light source about an arcuate path to selected positions therealong to alter the angle ofthe light into the optical element.

38. Apparatus as in claim 32 including a flow cell secured to said TIR surface, said flow cell including an inlet port and an outlet port for flowing analyte across said

TIR surface.

39. Apparatus as in claim 32 also including a computer, said computer including means for converting images from said reflected light into a convenient form for analysis.

40. Apparatus as in claim 36 wherein said filter means comprises a filter wheel located to intercept light from said light source, said apparatus including means for rotating said wheel.

41. Apparatus as in claim 36 wherein said filter means comprises a stack filter.

42. Apparatus as in claim 32 also including a plurality of imagers, each of said imagers being located at a position to capture an image generated in response to input light at a corresponding angle.

43. Apparatus as in claim 36 also including a plurality of imagers each of said imagers being located at a position to capture an image generated in response to input light of a corresponding wavelength.

44. Apparatus as in claim 38 wherein said flow cell includes means for securing a slide thereto.

45. Imaging Apparatus comprising an optical assembly, a light source assembly and at least one imaging detector, said assemblies being operative to provide an image reflected from a TIR surface defined by a top surface of said optical assembly, said light source assembly being operative to direct at said TIR surface polarized light in a manner to generate an evanescent field at said TIR surface, said optical assembly having a bottom surface, said bottom surface including first and second spaced apart gratings, said grating being located and configured to direct light passing through said first grating from said source to said TIR surface and light reflected from said TIR surface to said second grating to said detector respectively, said optical assembly being configured to impose on said light reflected from said TIR surface a superposition of images therefrom to provide a single reflection therefrom.

46. Apparatus as in claim 45 wherein said light is monochromatic.

47. Apparatus as in claim 45 including filter means for selecting a single image exiting said second grating.