WO2003062920A3 - Elastomeric stencils for micropatterning cells - Google Patents

Elastomeric stencils for micropatterning cells Download PDF

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Publication number
WO2003062920A3
WO2003062920A3 PCT/US2002/026003 US0226003W WO03062920A3 WO 2003062920 A3 WO2003062920 A3 WO 2003062920A3 US 0226003 W US0226003 W US 0226003W WO 03062920 A3 WO03062920 A3 WO 03062920A3
Authority
WO
WIPO (PCT)
Prior art keywords
stencil
substrate
cells
portions
stencils
Prior art date
Application number
PCT/US2002/026003
Other languages
French (fr)
Other versions
WO2003062920A2 (en
Inventor
Mehmet Toner
Albert Folch
David J Beebe
Original Assignee
Gen Hospital Corp
Univ Illinois
Mehmet Toner
Albert Folch
David J Beebe
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gen Hospital Corp, Univ Illinois, Mehmet Toner, Albert Folch, David J Beebe filed Critical Gen Hospital Corp
Priority to AU2002365249A priority Critical patent/AU2002365249A1/en
Publication of WO2003062920A2 publication Critical patent/WO2003062920A2/en
Publication of WO2003062920A3 publication Critical patent/WO2003062920A3/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/12—Specific details about manufacturing devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/069—Absorbents; Gels to retain a fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0819—Microarrays; Biochips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0893—Geometry, shape and general structure having a very large number of wells, microfabricated wells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials
    • B01L2300/123—Flexible; Elastomeric
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates

Abstract

The present invention provides new fabrication methods and techniques for making and using stencils to micropattern cells. These techniques may allow the fabrication of thinner stencils. The invention provides a system for selectively applying cells to predetermined regions on a substrate. A stencil is positioned adjacent to a substrate to cover some portions of the substrate, while allowing other portions of the substrate to remain uncovered. The stencil may have one or more channels passing through the stencil, and in certain cases, the channels may have a cross-sectional dimension of less than 1 mm. The stencil may be applied to a cell culture substrate in such a way as to form a microscopic cellular pattern on the substrate, and the stencil may form a seal or other watertight contact with the substrate in some cases. In one embodiment, the stencil may be applied to a substrate before seeding. Cells are applied to the uncovered portions of the surface, then the stencil may be removed if desired. The stencil may be fabricated, for example, by injecting an elastomeric precursor into a mold, or by curing an elastomeric precursor while applying pressure. In some embodiments, the surface of the stencil may also be oxidized, or it may be treated in such a way as to render the surface of the stencil more hydrophilic, for example, by treating the surface using oxygen-plasma treatment.
PCT/US2002/026003 2001-08-15 2002-08-15 Elastomeric stencils for micropatterning cells WO2003062920A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002365249A AU2002365249A1 (en) 2001-08-15 2002-08-15 Elastomeric stencils for micropatterning cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US31240501P 2001-08-15 2001-08-15
US60/312,405 2001-08-15

Publications (2)

Publication Number Publication Date
WO2003062920A2 WO2003062920A2 (en) 2003-07-31
WO2003062920A3 true WO2003062920A3 (en) 2003-10-09

Family

ID=27613173

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/026003 WO2003062920A2 (en) 2001-08-15 2002-08-15 Elastomeric stencils for micropatterning cells

Country Status (2)

Country Link
AU (1) AU2002365249A1 (en)
WO (1) WO2003062920A2 (en)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6893850B2 (en) 2000-03-17 2005-05-17 President And Fellows Of Harvard College Method for cell patterning
JP3584933B2 (en) 2002-10-08 2004-11-04 セイコーエプソン株式会社 Manufacturing method of microstructure, optical element, integrated circuit and electronic equipment
EP2161043A1 (en) * 2008-09-05 2010-03-10 Ecole Polytechnique Federale De Lausanne (Epfl) Coated medical device and method of coating a medical device
KR101346063B1 (en) * 2012-02-09 2013-12-31 정원재 Free-standing polymer membrane having through-hole and method of manufacturing the same
WO2014184687A1 (en) * 2013-05-17 2014-11-20 Indian Institute Of Technology Kanpur Microbe-based masters for micro contact printing and methods for their preparation and use
AU2016233335B2 (en) * 2015-03-17 2021-03-25 President And Fellows Of Harvard College Automated membrane fabrication system
MX2018001681A (en) 2015-08-10 2018-07-06 Essenlix Corp Bio/chemical assay devices and methods for simplified steps, small samples, accelerated speed, and ease-of-use.
CN111426822B (en) 2015-09-14 2022-12-13 艾森利克斯公司 Device and system for collecting and analyzing steam condensate, in particular exhaled gas condensate, and method of use
MX2018003148A (en) 2015-09-14 2019-02-20 Essenlix Corp Device and system for analyzing a sample, particularly blood, as well as methods of using the same.
US10628693B2 (en) 2016-12-21 2020-04-21 Essenlix Corporation Devices and methods for authenticating a sample and use of the same
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CN111316096B (en) 2017-02-08 2023-08-11 Essenlix公司 Biological/chemical material extraction and assay
CN110998325A (en) 2017-02-09 2020-04-10 Essenlix公司 Amplification assay
JP7004732B2 (en) 2017-02-09 2022-01-21 エッセンリックス コーポレーション Assay method using different spacing heights
WO2018148609A2 (en) 2017-02-09 2018-08-16 Essenlix Corporation Colorimetric assays
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WO2019028133A1 (en) 2017-08-01 2019-02-07 Essenlix Corporation Devices and methods for examining drug effects on microorganisms
US11280706B2 (en) 2017-08-01 2022-03-22 Essenlix Corporation Dilution calibration
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WO2019075415A1 (en) 2017-10-13 2019-04-18 Essenlix Corporation Devices and methods for authenticating a medical test and use of the same
US11609224B2 (en) 2017-10-26 2023-03-21 Essenlix Corporation Devices and methods for white blood cell analyses
US11237113B2 (en) 2017-10-26 2022-02-01 Essenlix Corporation Rapid pH measurement
US10807095B2 (en) 2017-10-26 2020-10-20 Essenlix Corporation Making and tracking assay card
US11648551B2 (en) 2017-12-12 2023-05-16 Essenlix Corporation Sample manipulation and assay with rapid temperature change
CN112534259A (en) 2017-12-14 2021-03-19 Essenlix公司 Device, system and method for monitoring hair
US11156606B2 (en) 2018-01-11 2021-10-26 Essenlix Corporation Homogeneous assay (II)
US11885952B2 (en) 2018-07-30 2024-01-30 Essenlix Corporation Optics, device, and system for assaying and imaging

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4022521A (en) * 1974-02-19 1977-05-10 Honeywell Inc. Microscope slide
US5324591A (en) * 1987-03-06 1994-06-28 Geo-Centers, Inc. Deep ultraviolet photolithographically defined ultra-thin films for selective cell adhesion and outgrowth and method of manufacturing the same and devices containing the same
US5512131A (en) * 1993-10-04 1996-04-30 President And Fellows Of Harvard College Formation of microstamped patterns on surfaces and derivative articles
US5593814A (en) * 1989-06-03 1997-01-14 Kanegafuchi Kagaku Kogyo Kabushiki Kaisha Control of cell arrangement

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4022521A (en) * 1974-02-19 1977-05-10 Honeywell Inc. Microscope slide
US5324591A (en) * 1987-03-06 1994-06-28 Geo-Centers, Inc. Deep ultraviolet photolithographically defined ultra-thin films for selective cell adhesion and outgrowth and method of manufacturing the same and devices containing the same
US5593814A (en) * 1989-06-03 1997-01-14 Kanegafuchi Kagaku Kogyo Kabushiki Kaisha Control of cell arrangement
US5512131A (en) * 1993-10-04 1996-04-30 President And Fellows Of Harvard College Formation of microstamped patterns on surfaces and derivative articles

Also Published As

Publication number Publication date
WO2003062920A2 (en) 2003-07-31
AU2002365249A1 (en) 2003-09-02

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