WO2003068919A3 - Non-invasive method for measuring rates of biosynthesis of biological molecules by label incorporation - Google Patents

Non-invasive method for measuring rates of biosynthesis of biological molecules by label incorporation Download PDF

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Publication number
WO2003068919A3
WO2003068919A3 PCT/US2003/004183 US0304183W WO03068919A3 WO 2003068919 A3 WO2003068919 A3 WO 2003068919A3 US 0304183 W US0304183 W US 0304183W WO 03068919 A3 WO03068919 A3 WO 03068919A3
Authority
WO
WIPO (PCT)
Prior art keywords
biosynthesis
biological molecules
invasive method
label incorporation
measuring rates
Prior art date
Application number
PCT/US2003/004183
Other languages
French (fr)
Other versions
WO2003068919A2 (en
Inventor
Marc K Hellerstein
Original Assignee
Univ California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ California filed Critical Univ California
Priority to AU2003217385A priority Critical patent/AU2003217385B2/en
Priority to EP03713429A priority patent/EP1483399B1/en
Priority to DE60324393T priority patent/DE60324393D1/en
Priority to JP2003568034A priority patent/JP2005517904A/en
Priority to CA2475924A priority patent/CA2475924C/en
Publication of WO2003068919A2 publication Critical patent/WO2003068919A2/en
Publication of WO2003068919A3 publication Critical patent/WO2003068919A3/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K51/00—Preparations containing radioactive substances for use in therapy or testing in vivo
    • A61K51/02—Preparations containing radioactive substances for use in therapy or testing in vivo characterised by the carrier, i.e. characterised by the agent or material covalently linked or complexing the radioactive nucleus
    • A61K51/04—Organic compounds
    • A61K51/0493—Steroids, e.g. cholesterol, testosterone
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/60—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances involving radioactive labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/52—Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/24—Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation

Abstract

Methods of determining rate of biosynthesis or breakdown of biological molecules from metabolic derivatives and catabolic products are disclosed herein. In particular, methods of measuring the rates of biosynthesis and breakdown of biological molecules inaccessible or not easily accessible to direct sampling by sampling metabolic derivatives and catabolic products in accessible biological samples are disclosed herein.
PCT/US2003/004183 2002-02-12 2003-02-12 Non-invasive method for measuring rates of biosynthesis of biological molecules by label incorporation WO2003068919A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
AU2003217385A AU2003217385B2 (en) 2002-02-12 2003-02-12 Non-invasive method for measuring rates of biosynthesis of biological molecules by label incorporation
EP03713429A EP1483399B1 (en) 2002-02-12 2003-02-12 Measurement of biosynthesis and breakdown rates of biological molecules that are inaccessible or not easily accessible to direct sampling, non-invasively, by label incorporation into metabolic derivatives and catabolitic products
DE60324393T DE60324393D1 (en) 2002-02-12 2003-02-12 NONINVASIVE MEASUREMENT OF BIOSYNTHESIS AND DEFROSTING SPEEDS OF BIOLOGICAL MOLECULES WHICH ARE ACCESSIBLE TO A DIRECT SAMPLING, OR NOT EASILY ACCESSIBLE, BY INSTALLING A MARKER IN METABOLIC DERIVATIVES AND KATABOLE PRODUCTS
JP2003568034A JP2005517904A (en) 2002-02-12 2003-02-12 A non-invasive method for measuring the rate of biosynthesis of biological molecules by label incorporation
CA2475924A CA2475924C (en) 2002-02-12 2003-02-12 Non-invasive method for measuring rates of biosynthesis of biological molecules by label incorporation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US35600802P 2002-02-12 2002-02-12
US60/356,008 2002-02-12

Publications (2)

Publication Number Publication Date
WO2003068919A2 WO2003068919A2 (en) 2003-08-21
WO2003068919A3 true WO2003068919A3 (en) 2004-08-19

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/004183 WO2003068919A2 (en) 2002-02-12 2003-02-12 Non-invasive method for measuring rates of biosynthesis of biological molecules by label incorporation

Country Status (9)

Country Link
US (2) US7449171B2 (en)
EP (1) EP1483399B1 (en)
JP (1) JP2005517904A (en)
AT (1) ATE412906T1 (en)
AU (1) AU2003217385B2 (en)
CA (1) CA2475924C (en)
DE (1) DE60324393D1 (en)
TW (1) TWI263677B (en)
WO (1) WO2003068919A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111007170B (en) * 2019-12-13 2021-06-29 中国农业科学院农产品加工研究所 Biomarker for intervention treatment of osteoporosis by bone peptide, screening method and application

Families Citing this family (56)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5910403A (en) * 1997-05-15 1999-06-08 The Regents Of University Of California Methods for measuring cellular proliferation and destruction rates in vitro and in vivo
AU2002365268B2 (en) * 2001-10-24 2008-02-28 The Regents Of The University Of California Measurement of protein synthesis rates in humans and experimental systems by use of isotopically labeled water
US7449171B2 (en) * 2002-02-12 2008-11-11 The Regents Of The University Of California Measurement of biosynthesis and breakdown rates of biological molecules that are inaccessible or not easily accessible to direct sampling, non-invasively, by label incorporation into metabolic derivatives and catabolic products
AU2003234688A1 (en) * 2002-04-05 2003-10-27 The Regents Of The University Of California Method for isolating and measuring proliferation of long-term label retaining cells and stem cells
CA2494715C (en) * 2002-07-30 2014-07-08 The Regents Of The University Of California Method for automated, large-scale measurement of the molecular flux rates using mass spectrometry
WO2004021863A2 (en) * 2002-09-04 2004-03-18 The Regents Of The University Of California Methods for measuring rates of replication and death of nfectious microbial agents in an infected host organism
DE60324950D1 (en) 2002-09-13 2009-01-08 Univ California METHOD OF MEASURING THE SPEEDS OF CHOLESTER REVERSE TRANSPORT IN VIVO AS AN INDEX FOR ANTI-ARTHEROGENESIS
US20070248540A1 (en) * 2002-09-16 2007-10-25 The Regents Of The University Of California Biochemical methods for measuring metabolic fitness of tissues or whole organisms
US20040081994A1 (en) * 2002-09-16 2004-04-29 Hellerstein Marc K. Biochemical methods for measuring metabolic fitness of tissues or whole organisms
CA2504313C (en) 2002-11-04 2012-01-17 Marc K. Hellerstein Deuterated glucose or fat tolerance tests for high-throughput measurement of the metabolism of sugars or fatty acids in the body
US7262020B2 (en) * 2003-07-03 2007-08-28 The Regents Of The University Of California Methods for comparing relative flux rates of two or more biological molecules in vivo through a single protocol
US20050202406A1 (en) * 2003-11-25 2005-09-15 The Regents Of The University Of California Method for high-throughput screening of compounds and combinations of compounds for discovery and quantification of actions, particularly unanticipated therapeutic or toxic actions, in biological systems
TW200538738A (en) 2004-02-20 2005-12-01 Univ California Molecular flux rates through critical pathways measured by stable isotope labeling in vivo, as biomarkers of drug action and disease activity
WO2005087943A1 (en) * 2004-03-11 2005-09-22 The Regents Of The University Of California Temporal or spatial characterization of biosynthetic events in living organisms by isotopic fingerprinting under conditions of imposed isotopic gradients
US20050238577A1 (en) * 2004-03-29 2005-10-27 The Regents Of The University Of California Isolation of epithelial cells or their biochemical contents from excreta after in vivo isotopic labeling
AU2005271264A1 (en) * 2004-08-07 2006-02-16 Kinemed, Inc. Method for measuring dynamics of self-assembling systems of biological molecules in vivo and uses for discovering or evaluating therapeutic agents
DK1886112T3 (en) * 2005-04-06 2014-09-08 Univ St Louis Procedure for Measuring the Metabolism of Neurally Derived Biomolecules in Vivo
JP2006292683A (en) * 2005-04-14 2006-10-26 National Institute Of Advanced Industrial & Technology Identification method of oligosaccharide derived from keratan sulfate
US20060251576A1 (en) * 2005-05-03 2006-11-09 The Regents Of The University Of California Methods for measuring cholesterol metabolism and transport
TW200711660A (en) * 2005-06-10 2007-04-01 Univ California Monitoring two dimensions of diabetes pathogenesis separately or concurrently (insulin sensitivity and beta-cell sufficiency): uses in diagnosis, prognosis, assessment of disease risk, and drug development
FI20050814A0 (en) * 2005-08-11 2005-08-11 Procollagen Oy Procedure for observing autoantibodies formed in rheumatoid arthritis
WO2007035397A1 (en) * 2005-09-15 2007-03-29 The Regents Of The University Of Michigan Method and system for measuring cartilage condition biomarkers
AU2006299406A1 (en) * 2005-09-30 2007-04-12 Kinemed, Inc. Microtubule synthesis as a biomarker
CA2636333A1 (en) 2006-01-05 2007-07-19 Kinemed, Inc. Compositions and methods of treatment using modulators of motonueron diseases
GB0612669D0 (en) * 2006-06-27 2006-08-09 Univ Leeds Biomarkers for preeclampsia
WO2009062152A1 (en) * 2007-11-09 2009-05-14 Washington University In St. Louis Methods for measuring the metabolism of cns derived biomolecules in vivo
AU2009248971B2 (en) * 2008-05-21 2015-04-30 Kinemed, Inc. Compositions and methods of treatment using modulators of motoneuron diseases
WO2009153783A1 (en) * 2008-06-16 2009-12-23 Mor Research Applications Ltd. Monitoring skin metabolism products for evaluating burn injury
US20110288114A1 (en) * 2008-06-20 2011-11-24 Kinemed, Inc. Compositions for the treatment of fibrotic diseases or conditions
JP2012508886A (en) * 2008-11-12 2012-04-12 ザ ワシントン ユニバーシティ Simultaneous measurement of in vivo metabolism of biomolecular isoforms
DK2373988T3 (en) * 2008-12-05 2015-05-11 C2N Diagnostics Methods of measuring concentrations of biomolecules
US8809009B2 (en) 2009-01-02 2014-08-19 Biomarin Pharmaceutical Inc. Methods of diagnosing a disease and methods of monitoring treatment of a disease by quantifying a non-reducing end glycan residual compound and comparing to a second biomarker
US9029530B2 (en) * 2009-01-02 2015-05-12 Biomarin Pharmaceutical Inc. Detection of oligosaccharides
WO2010078515A2 (en) * 2009-01-02 2010-07-08 Zacharon Pharmaceuticals, Inc. Polymer end group detection
US8232073B2 (en) 2009-01-02 2012-07-31 Zacharon Pharmaceuticals, Inc. Quantification of non-reducing end glycan residual compounds
US20100248380A1 (en) * 2009-03-13 2010-09-30 Arete Therapeutics, Inc. Method and assays for quantitation of acetamide in a composition
EP2575596B1 (en) * 2010-05-24 2016-04-20 The Washington University Methods of determining amyloid beta turnover in blood
US9110008B2 (en) 2010-07-26 2015-08-18 Los Gatos Research Method for isotopic analysis of water in bodily fluids
BR112013008701B1 (en) * 2010-10-13 2021-08-03 Council Of Scientific & Industrial Research METHOD OF DETECTION OF THE PRESENCE OF VALVULAR HEART DISEASE OF RHEUMATIC ORIGIN IN AN INDIVIDUAL
US10814018B2 (en) 2010-10-25 2020-10-27 Hadasit Medical Research Service & Development Ltd. Isotopically labeled deoxy-glucose and derivatives thereof, compositions comprising them and uses thereof
JP6323978B2 (en) 2010-12-24 2018-05-16 アークレイ株式会社 Method for detecting cancer cells
WO2013036885A1 (en) 2011-09-08 2013-03-14 The Regents Of The University Of California Metabolic flux measurement, imaging and microscopy
AU2012345902A1 (en) * 2011-12-02 2014-06-26 C2N Diagnostics Methods for measuring concentrations of biomolecules
ES2668678T3 (en) 2011-12-07 2018-05-21 Glaxosmithkline Llc Procedure for determining total body skeletal muscle mass
US20150140672A1 (en) * 2011-12-19 2015-05-21 Washington University Methods for detecting amyloid beta amyloidosis
US20140370619A1 (en) * 2011-12-19 2014-12-18 Washington University Methods for diagnosing alzheimer's disease
JP5845236B2 (en) * 2012-11-29 2016-01-20 株式会社ニッピ Method for analyzing collagen and method for producing stable isotope-labeled collagen
US9134319B2 (en) 2013-03-15 2015-09-15 The Regents Of The University Of California Method for replacing biomarkers of protein kinetics from tissue samples by biomarkers of protein kinetics from body fluids after isotopic labeling in vivo
ES2705687T3 (en) * 2013-11-05 2019-03-26 Tepha Inc Poly-4-hydroxybutyrate compositions and devices
AU2014352672B2 (en) * 2013-11-25 2018-11-29 Deuteria Biomaterials, Llc Deuterium-enriched hyaluronan
US20170067921A1 (en) * 2014-05-08 2017-03-09 John P. Jasper Isotopic identification and tracing of biologic products
EP3760235A1 (en) 2014-09-30 2021-01-06 Washington University Tau kinetic measurements
US11061005B2 (en) * 2017-04-20 2021-07-13 Metabolon, Inc. Mass spectrometry assay method for detection and quantitation of organic acid metabolites
US11085935B2 (en) 2018-05-03 2021-08-10 Washington University Methods of treating based on site-specific tau phosphorylation
CN108663449A (en) * 2018-05-15 2018-10-16 安徽相王医疗健康股份有限公司 Albumen parameters analysis method in a kind of Coal Workers Pneumoconiosis serum
US11754478B2 (en) 2018-08-16 2023-09-12 Abb Schweiz Ag Rapid equilibrator for water isotope analysis

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6010846A (en) * 1997-05-15 2000-01-04 The Regents Of The University Of California Methods for measuring cellular proliferation and destruction rates in vitro and in vivo

Family Cites Families (97)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4065552A (en) 1975-05-05 1977-12-27 Giovanni Giacomo Costa Method of detecting malignant neoplasms
US4332784A (en) 1979-02-06 1982-06-01 The Radiochemical Centre Limited Dual isotope assays
SU968036A1 (en) 1980-03-10 1982-10-23 Научно-исследовательский институт медицинской радиологии АМН СССР Method for preparing marked protein
US5317098A (en) 1986-03-17 1994-05-31 Hiroaki Shizuya Non-radioisotope tagging of fragments
DE3667103D1 (en) 1986-06-09 1989-12-28 Bruker Analytische Messtechnik Method for determining the turnover of organic material in living tissue and nmr spectrometer for performing this method
US4940658A (en) 1986-11-20 1990-07-10 University Patents, Inc. Assay for sulfhydryl amino acids and methods for detecting and distinguishing cobalamin and folic acid deficency
WO1989001342A2 (en) 1987-08-07 1989-02-23 Mallinckrodt, Inc. Diagnostic or radiotherapeutic composition comprising a hydrogen containing compound
US5042488A (en) 1987-09-29 1991-08-27 The Washington University Methods employing deuterium for obtaining direct, observable deuterium magnetic resonance images in vivo and in situ
US6004781A (en) * 1988-01-22 1999-12-21 The General Hospital Corporation Nucleic acid encoding Ig-CD4 fusion proteins
WO1990011371A1 (en) 1989-03-20 1990-10-04 Anticancer Inc. NATIVE-STATE METHOD AND SYSTEM FOR DETERMINING VIABILITY AND PROLIFERATIVE CAPACITY OF TISSUES $i(IN VITRO)
GB2229718B (en) * 1989-03-22 1993-01-06 Inst Molekularnoi Genetik Method for preparing hydrogen-isotape labelled biologically active organic compounds
US5217903A (en) 1990-05-15 1993-06-08 Trustees Of Boston University Measuring connective tissue breakdown products in body fluids
CA2027714C (en) 1990-07-13 2003-01-28 Kenneth W. Turtletaub Method of measurement in biological systems
US5209919A (en) 1990-07-13 1993-05-11 Regents Of The University Of California Method of measurement in biological systems
US5597548A (en) 1990-07-18 1997-01-28 Board Of Regents, The University Of Texas System 13 C Isotopomer analyses in intact tissue using (13 C) homonuclear decoupling
HU208084B (en) * 1991-10-31 1993-08-30 Hyd Kutato Fejlesztoe Kft Process for producing compositions suitable for curing tumorous diseases
US5394236A (en) 1992-02-03 1995-02-28 Rutgers, The State University Methods and apparatus for isotopic analysis
WO1993020800A1 (en) 1992-04-08 1993-10-28 Paavo Kai Johannes Kinnunen Composition for therapeutic or diagnostic use, process for its preparation and its use
US5338686A (en) * 1992-04-29 1994-08-16 Hellerstein Marc K Method for measuring in vivo synthesis of biopolymers
JP2960832B2 (en) * 1992-05-08 1999-10-12 ペルマテック テクノロジー アクチェンゲゼルシャフト Estradiol administration system
US5366885A (en) 1992-06-09 1994-11-22 Barranco Iii Sam C Method and kit for testing tumors for drug sensitivity
US5432058A (en) * 1992-09-30 1995-07-11 Lange, Iii; Louis G. Method for measuring human cholesterol absorption using metabolically stable isotopes
US5345662A (en) * 1993-03-08 1994-09-13 Langis Lambert Apparatus for inserting a spline into a frame groove
US5506147A (en) 1993-04-15 1996-04-09 Kolhouse; J. Fred Non-invasive evaluation of maldigestion and malaborption
DE650396T1 (en) * 1993-05-17 1995-11-30 Amersham Int Plc DEVICE AND METHOD FOR DETECTING CELLULAR AND BIOCHEMICAL PROCESSES.
US5439803A (en) 1993-08-13 1995-08-08 The Regents Of The University Of Michigan Isotope and assay for glycolysis and the pentose phosphate pathway
US5756067A (en) 1993-11-08 1998-05-26 Peptide Delivery Systems Pty Ltd Labelled diagnostic compositions and method of their use
MX9702602A (en) 1995-08-08 1997-07-31 Otsuka Pharma Co Ltd Diagnostic and diagnosis method for central nervous abnormality and phenylketonuria.
US5922554A (en) * 1995-10-30 1999-07-13 The Regents Of The University Of California Inhibition of cellular uptake of cholesterol
CA2213935C (en) 1996-08-27 2002-10-01 Tokyo Gas Co., Ltd. Diagnostic agent for diabetes
EP0979306A4 (en) 1997-02-14 2004-09-01 Univ George Washington An assay for the measurement of dna synthesis rates
CN1127118C (en) 1997-03-14 2003-11-05 乔治华盛顿大学 Device for continuous isotope ratio monitoring following fluorine based chemical reactions
US5961470A (en) 1997-07-09 1999-10-05 Wagner; David A. Breath test for assessing hepatic function
US6329208B1 (en) * 1997-07-16 2001-12-11 Board Of Regents, The University Of Texas System Methods for determining gluconeogenesis, anapleurosis and pyruvate recycling
CA2249173C (en) 1997-10-06 2008-12-16 Tokyo Gas Co., Ltd. Diagnostic agent for liver function
US5924995A (en) 1997-11-10 1999-07-20 Meretek Diagnostics Non-invasive method for the functional assessment of infants and children with an inherited metabolic disorder
US6461870B2 (en) 1998-05-06 2002-10-08 Isotechnika Inc. 13C glucose breath test for the diagnosis of diabetic indications and monitoring glycemic control
ES2161669T1 (en) 1998-05-06 2001-12-16 Isotechnika Inc GLUCOSE TEST 13C PER BREATH FOR THE DIAGNOSIS OF DIABETES INDICATIONS AND MONITORING OF GLYCEMIC CONTROL.
US6284219B1 (en) * 1998-06-30 2001-09-04 Phenome Sciences Inc. In vivo determination of metabolic function for use in therapy management
US6625547B1 (en) 1998-08-05 2003-09-23 Washington State University Research Foundation Relative rates of cytochrome p450 metabolism
DE69912444T3 (en) * 1998-08-25 2010-05-06 University Of Washington, Seattle FAST QUANTITATIVE ANALYSIS OF PROTEINS OR PROTEIN FUNCTIONS IN COMPLEX MIXTURES
DE19839491A1 (en) 1998-08-29 2000-03-02 Hermann Heumann Process for labeling biopolymers with isotopes
JP2002523114A (en) 1998-08-31 2002-07-30 ユニバーシテイ オブ ワシントン Stable isotope metabolic labeling for biopolymer analysis
US6693186B2 (en) 1998-09-01 2004-02-17 Antex Biologics Inc Neisseria meningitidis polypeptide, nucleic acid sequence and uses thereof
US6887712B1 (en) 1998-11-09 2005-05-03 Atherogenics, Inc. Methods and compositions to lower plasma cholesterol levels
US6764817B1 (en) 1999-04-20 2004-07-20 Target Discovery, Inc. Methods for conducting metabolic analyses
US20030119069A1 (en) 1999-04-20 2003-06-26 Target Discovery, Inc. Labeling of protein samples
IL146093A0 (en) 1999-04-20 2002-07-25 Target Discovery Inc Method of separating a polypeptide or protein from a solution containing a plurality thereof
US6391649B1 (en) 1999-05-04 2002-05-21 The Rockefeller University Method for the comparative quantitative analysis of proteins and other biological material by isotopic labeling and mass spectroscopy
US7057168B2 (en) 1999-07-21 2006-06-06 Sionex Corporation Systems for differential ion mobility analysis
US6902712B2 (en) * 1999-10-12 2005-06-07 General Hospital Supply Corporation Absorbent liner for sterilization process and method of sterilizing surgical instruments
US6653090B1 (en) 1999-11-05 2003-11-25 University Of Alberta Methods for measuring the metabolism of and screening for drugs in isolated hearts
JP2001211782A (en) 2000-02-02 2001-08-07 Masa Yamamoto Tob gene deletion knock out non-human mammal
CA2405722A1 (en) 2000-04-13 2001-11-08 Thermo Finnigan Llc Proteomic analysis by parallel mass spectrometry
ATE344456T1 (en) 2000-05-05 2006-11-15 Purdue Research Foundation AFFINITY-SELECTED SIGNATURE EPPTIDES FOR IDENTIFYING AND QUANTIFYING PROTEINS
JP2003533680A (en) 2000-05-18 2003-11-11 メタボリック ソリューションズ，インコーポレイティド Reverse isotope dilution assay and lactose intolerance assay
US6680203B2 (en) 2000-07-10 2004-01-20 Esperion Therapeutics, Inc. Fourier transform mass spectrometry of complex biological samples
US7048907B2 (en) 2001-02-05 2006-05-23 Biophysics Assay Laboratory, Inc. Synthesis, compositions and methods for the measurement of the concentration of stable-isotope labeled compounds in life forms and life form excretory products
GB0106923D0 (en) 2001-03-20 2001-05-09 Univ Dundee Liver function test
US7256047B2 (en) 2001-05-01 2007-08-14 Board Of Regents, The University Of Texas System Measurement of gluconeogenesis and intermediary metabolism using stable isotopes
EP1421198A4 (en) 2001-06-26 2006-08-30 New York State Office Of Menta Cell-based high-throughput screening methods
US6835927B2 (en) 2001-10-15 2004-12-28 Surromed, Inc. Mass spectrometric quantification of chemical mixture components
AU2002365268B2 (en) 2001-10-24 2008-02-28 The Regents Of The University Of California Measurement of protein synthesis rates in humans and experimental systems by use of isotopically labeled water
ATE403872T1 (en) 2001-12-08 2008-08-15 Micromass Ltd MASS SPECTORMETRY METHOD
US7635841B2 (en) 2001-12-12 2009-12-22 Micromass Uk Limited Method of mass spectrometry
US7449171B2 (en) * 2002-02-12 2008-11-11 The Regents Of The University Of California Measurement of biosynthesis and breakdown rates of biological molecules that are inaccessible or not easily accessible to direct sampling, non-invasively, by label incorporation into metabolic derivatives and catabolic products
US20030180710A1 (en) 2002-03-22 2003-09-25 Lee Wai-Nang Paul Method of enhancing the efficiency of a pharmaceutical business
US20060100903A1 (en) 2002-03-22 2006-05-11 Los Angeles Biomedical Research Institute At Harbor-Ucla Medical Center Method of enhancing the efficiency of a pharmaceutical business
US20050281745A1 (en) 2002-03-22 2005-12-22 Los Angeles Biomedical Research Institute At Harbor-Ucla Medical Center Stable isotope based dynamic metabolic profiling of living organisms for characterization of metabolic diseases, drug testing and drug development
US20030180800A1 (en) 2002-03-22 2003-09-25 Lee Wai-Nang Paul Stable isotope based dynamic metabolic profiling of living organisms for characterization of metabolic diseases, drug testing and drug development
AU2003234688A1 (en) 2002-04-05 2003-10-27 The Regents Of The University Of California Method for isolating and measuring proliferation of long-term label retaining cells and stem cells
US20030211036A1 (en) 2002-05-07 2003-11-13 Hadassa Degani Method and apparatus for monitoring and quantitatively evaluating tumor perfusion
US20030228059A1 (en) * 2002-06-06 2003-12-11 Atul Khanduri Data collator for a plurality of asynchronous/synchronous data generation entities
US7510880B2 (en) 2002-06-26 2009-03-31 Gross Richard W Multidimensional mass spectrometry of serum and cellular lipids directly from biologic extracts
CA2494715C (en) 2002-07-30 2014-07-08 The Regents Of The University Of California Method for automated, large-scale measurement of the molecular flux rates using mass spectrometry
AU2003265448A1 (en) 2002-08-16 2004-03-03 The Regents Of The University Of California Dynamic hepatic recycling glucose tolerance test
WO2004021863A2 (en) 2002-09-04 2004-03-18 The Regents Of The University Of California Methods for measuring rates of replication and death of nfectious microbial agents in an infected host organism
DE60324950D1 (en) 2002-09-13 2009-01-08 Univ California METHOD OF MEASURING THE SPEEDS OF CHOLESTER REVERSE TRANSPORT IN VIVO AS AN INDEX FOR ANTI-ARTHEROGENESIS
US20040081994A1 (en) 2002-09-16 2004-04-29 Hellerstein Marc K. Biochemical methods for measuring metabolic fitness of tissues or whole organisms
US20070248540A1 (en) * 2002-09-16 2007-10-25 The Regents Of The University Of California Biochemical methods for measuring metabolic fitness of tissues or whole organisms
EP2595173B1 (en) 2002-10-29 2016-09-14 Target Discovery, Inc. Method for increasing ionization efficiency in mass spectroscopy
CA2504313C (en) 2002-11-04 2012-01-17 Marc K. Hellerstein Deuterated glucose or fat tolerance tests for high-throughput measurement of the metabolism of sugars or fatty acids in the body
US20040121305A1 (en) 2002-12-18 2004-06-24 Wiegand Roger Charles Generation of efficacy, toxicity and disease signatures and methods of use thereof
WO2004089972A2 (en) 2003-04-02 2004-10-21 Merck & Co., Inc. Mass spectrometry data analysis techniques
US20050014181A1 (en) 2003-06-18 2005-01-20 Galis Zorina S. Methods and compositions for the assessment of polymer assembly
EP1644856A2 (en) 2003-06-30 2006-04-12 Ajinomoto Co., Inc. Intracellular metabolic flux analysis method using substrate labeled with isotope
US7262020B2 (en) 2003-07-03 2007-08-28 The Regents Of The University Of California Methods for comparing relative flux rates of two or more biological molecules in vivo through a single protocol
US20050202406A1 (en) 2003-11-25 2005-09-15 The Regents Of The University Of California Method for high-throughput screening of compounds and combinations of compounds for discovery and quantification of actions, particularly unanticipated therapeutic or toxic actions, in biological systems
US8510054B2 (en) 2004-02-05 2013-08-13 Ajinomoto Co., Inc. Intracellular metabolic flux analysis method using substrate labeled with isotope
TW200538738A (en) 2004-02-20 2005-12-01 Univ California Molecular flux rates through critical pathways measured by stable isotope labeling in vivo, as biomarkers of drug action and disease activity
WO2005087943A1 (en) 2004-03-11 2005-09-22 The Regents Of The University Of California Temporal or spatial characterization of biosynthetic events in living organisms by isotopic fingerprinting under conditions of imposed isotopic gradients
US20050238577A1 (en) 2004-03-29 2005-10-27 The Regents Of The University Of California Isolation of epithelial cells or their biochemical contents from excreta after in vivo isotopic labeling
WO2005098024A1 (en) * 2004-03-30 2005-10-20 Kinemed, Inc. In vivo measurement of the relative fluxes through ribonucleotide reductase vs. deoxyribonucleoside pathways using isotopes
JP4654592B2 (en) 2004-04-02 2011-03-23 味の素株式会社 Determination of metabolic flux
WO2006050130A2 (en) 2004-10-29 2006-05-11 Target Discovery, Inc. Glycan analysis using deuterated glucose
US20060251576A1 (en) 2005-05-03 2006-11-09 The Regents Of The University Of California Methods for measuring cholesterol metabolism and transport
TW200711660A (en) 2005-06-10 2007-04-01 Univ California Monitoring two dimensions of diabetes pathogenesis separately or concurrently (insulin sensitivity and beta-cell sufficiency): uses in diagnosis, prognosis, assessment of disease risk, and drug development

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6010846A (en) * 1997-05-15 2000-01-04 The Regents Of The University Of California Methods for measuring cellular proliferation and destruction rates in vitro and in vivo

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
HELLERSTEIN M.K. ET AL: "Mass isotopomer distribution analysis at eight years: theoretical, analytic and experimental considerations", AM. J. OF PHYSIOLOGY, vol. 263, November 1992 (1992-11-01), pages E1146 - E1170, XP002978087 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111007170B (en) * 2019-12-13 2021-06-29 中国农业科学院农产品加工研究所 Biomarker for intervention treatment of osteoporosis by bone peptide, screening method and application

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