WO2003084385A3 - Redifferentiated cells for repairing cartilage defects - Google Patents

Redifferentiated cells for repairing cartilage defects Download PDF

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Publication number
WO2003084385A3
WO2003084385A3 PCT/US2003/010076 US0310076W WO03084385A3 WO 2003084385 A3 WO2003084385 A3 WO 2003084385A3 US 0310076 W US0310076 W US 0310076W WO 03084385 A3 WO03084385 A3 WO 03084385A3
Authority
WO
WIPO (PCT)
Prior art keywords
proteoglycan
cell
fibroblast
cartilage defects
cells
Prior art date
Application number
PCT/US2003/010076
Other languages
French (fr)
Other versions
WO2003084385A2 (en
Inventor
Margaret M French
Kyriacos A Athanasiou
Original Assignee
Univ Rice William M
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Rice William M filed Critical Univ Rice William M
Priority to AU2003226201A priority Critical patent/AU2003226201A1/en
Priority to JP2003581639A priority patent/JP2005531298A/en
Priority to EP03746108A priority patent/EP1490477A4/en
Priority to CA002479840A priority patent/CA2479840A1/en
Publication of WO2003084385A2 publication Critical patent/WO2003084385A2/en
Publication of WO2003084385A3 publication Critical patent/WO2003084385A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0655—Chondrocytes; Cartilage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/105—Insulin-like growth factors [IGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
    • C12N2506/1307—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Rheumatology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Materials For Medical Uses (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A redifferentiated dermal fibroblast cell that exhibits at least one characteristic of a chondrocyte. A proteoglycan is used to induce re-differentiation of the cell. In some embodiments, the cell expresses of at least one cartilage proteoglycan marker. The proteoglycan may comprise aggrecan and the cell may differentiate from the fibroblast along the chondrogenic lineage. A method of inducing chondrogenesis in a fibroblast cell comprises culturing the fibroblast cell on a surface containing at least one cartilage-derived proteoglycan other than perlecan. A three-dimensional scaffold may be coated with the proteoglycan and seeded with fibroblast cells. The fibroblast cells may be contacted with at least one chondrogenic growth factor or cytokine prior to said culturing.
PCT/US2003/010076 2002-04-02 2003-04-02 Redifferentiated cells for repairing cartilage defects WO2003084385A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU2003226201A AU2003226201A1 (en) 2002-04-02 2003-04-02 Redifferentiated cells for repairing cartilage defects
JP2003581639A JP2005531298A (en) 2002-04-02 2003-04-02 Redifferentiated cells to repair cartilage defects
EP03746108A EP1490477A4 (en) 2002-04-02 2003-04-02 Redifferentiated cells for repairing cartilage defects
CA002479840A CA2479840A1 (en) 2002-04-02 2003-04-02 Redifferentiated cells for repairing cartilage defects

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US36942102P 2002-04-02 2002-04-02
US60/369,421 2002-04-02

Publications (2)

Publication Number Publication Date
WO2003084385A2 WO2003084385A2 (en) 2003-10-16
WO2003084385A3 true WO2003084385A3 (en) 2004-04-01

Family

ID=28791950

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/010076 WO2003084385A2 (en) 2002-04-02 2003-04-02 Redifferentiated cells for repairing cartilage defects

Country Status (6)

Country Link
US (1) US20030215426A1 (en)
EP (1) EP1490477A4 (en)
JP (1) JP2005531298A (en)
AU (1) AU2003226201A1 (en)
CA (1) CA2479840A1 (en)
WO (1) WO2003084385A2 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6755863B2 (en) * 1999-10-08 2004-06-29 Bret A. Ferree Rotator cuff repair using engineered tissues
US7435260B2 (en) * 1999-08-13 2008-10-14 Ferree Bret A Use of morphogenetic proteins to treat human disc disease
US9526746B2 (en) 2004-02-13 2016-12-27 Smith & Nephew, Inc. Wound healing composition
US8637065B2 (en) * 2004-07-09 2014-01-28 William Marsh Rice University Dermis-derived cells for tissue engineering applications
US7931894B2 (en) 2004-07-30 2011-04-26 Otsuka Pharmaceutical Co., Ltd. Method of three-dimensionally culturing chondrocytes
KR100900360B1 (en) * 2004-08-24 2009-06-02 후지소프트 가부시키가이샤 Medium for the redifferentiation of dedifferentiated chondrocytes into chondrocytes
US8017394B2 (en) * 2004-10-01 2011-09-13 Isto Technologies, Inc. Method for chondrocyte expansion with phenotype retention
US7273756B2 (en) * 2004-10-01 2007-09-25 Isto Technologies, Inc. Method for chondrocyte expansion with phenotype retention
GB0505202D0 (en) 2005-03-14 2005-04-20 Intercytex Ltd Skin equivalent culture
AU2006251004C1 (en) 2005-05-26 2013-05-23 Intercytex Limited Tissue repair using allogenic dermal fibroblasts
CA2648327A1 (en) * 2006-04-05 2007-10-11 William Marsh Rice University Tissue engineering with human embryonic stem cells
JP6099312B2 (en) * 2012-03-14 2017-03-22 国立大学法人 和歌山大学 Proteoglycan-immobilized organic material

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6133230A (en) * 1995-10-23 2000-10-17 Queen's University At Kingston Method and pharmaceutical composition for chondrostimulation with a prostaglandin (e.g. misoprostol) and TGF-β, optionally in combination with IGF-1

Family Cites Families (14)

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Publication number Priority date Publication date Assignee Title
US4829000A (en) * 1985-08-30 1989-05-09 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Reconstituted basement membrane complex with biological activity
US5272138A (en) * 1988-02-12 1993-12-21 The Biomembrane Institute Naturally occurring gangliosides containing de-N-acetyl-sialic acid and their applications as modifiers of cell physiology
US5356630A (en) * 1989-02-22 1994-10-18 Massachusetts Institute Of Technology Delivery system for controlled release of bioactive factors
US5206023A (en) * 1991-01-31 1993-04-27 Robert F. Shaw Method and compositions for the treatment and repair of defects or lesions in cartilage
US5723331A (en) * 1994-05-05 1998-03-03 Genzyme Corporation Methods and compositions for the repair of articular cartilage defects in mammals
AU730749B2 (en) * 1996-07-25 2001-03-15 Genzyme Corporation Chondrocyte media formulations and culture procedures
EP0893493A3 (en) * 1997-07-21 2002-12-04 Aventis Pharma Deutschland GmbH Genetically modified cells and their use for prophylaxis or treatment of diseases
EP0896825B1 (en) * 1997-08-14 2002-07-17 Sulzer Innotec Ag Composition and device for in vivo cartilage repair comprising nanocapsules with osteoinductive and/or chondroinductive factors
CN100529063C (en) * 1997-12-02 2009-08-19 泽恩比奥公司 Differentiation of adipose stromal cells into osteobalsts and uses thereof
US6197586B1 (en) * 1997-12-12 2001-03-06 The Regents Of The University Of California Chondrocyte-like cells useful for tissue engineering and methods
US6197061B1 (en) * 1999-03-01 2001-03-06 Koichi Masuda In vitro production of transplantable cartilage tissue cohesive cartilage produced thereby, and method for the surgical repair of cartilage damage
US6548058B1 (en) * 1999-07-20 2003-04-15 Epitech, S.A. Keratinocyte culture and uses thereof
US6429013B1 (en) * 1999-08-19 2002-08-06 Artecel Science, Inc. Use of adipose tissue-derived stromal cells for chondrocyte differentiation and cartilage repair
KR100394430B1 (en) * 1999-12-14 2003-08-09 (주)이노테크 메디칼 Medium for culturing human cell comprising human serum and method for culturing human cell using same

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6133230A (en) * 1995-10-23 2000-10-17 Queen's University At Kingston Method and pharmaceutical composition for chondrostimulation with a prostaglandin (e.g. misoprostol) and TGF-β, optionally in combination with IGF-1

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
FRENCH ET AL.: "Expression of the heparin sulfate proteoglycan, perlecan, during mouse embryogenesis and perlecan chondrogenic activity in vitro", THE JOURNAL OF CELL BIOLOGY, vol. 145, no. 5, May 1999 (1999-05-01), pages 1103 - 1115, XP002906978 *
GLOWACKI ET AL.: "Induced chodroblastic differentiation of human fibroblasts by three dimensional culture with demineralized bone matrix", MATERIALS SCIENCE AND ENGINEERING, vol. 6, no. 4, 1998, pages 199 - 203, XP002966189 *
See also references of EP1490477A4 *
YANG ET AL.: "Aggrecan and link protein affect cell adhesion to culture plates and to type II collagen", MATRIX BIOLOGY, vol. 16, no. 9, 1998, pages 541 - 561, XP002970806 *

Also Published As

Publication number Publication date
JP2005531298A (en) 2005-10-20
WO2003084385A2 (en) 2003-10-16
AU2003226201A1 (en) 2003-10-20
AU2003226201A8 (en) 2003-10-20
EP1490477A4 (en) 2006-08-30
US20030215426A1 (en) 2003-11-20
EP1490477A2 (en) 2004-12-29
CA2479840A1 (en) 2003-10-16

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