WO2003084976A1 - Process for isolating nucleic acid with chaotrope agents and ammonium compounds - Google Patents
Process for isolating nucleic acid with chaotrope agents and ammonium compounds Download PDFInfo
- Publication number
- WO2003084976A1 WO2003084976A1 PCT/IB2003/001202 IB0301202W WO03084976A1 WO 2003084976 A1 WO2003084976 A1 WO 2003084976A1 IB 0301202 W IB0301202 W IB 0301202W WO 03084976 A1 WO03084976 A1 WO 03084976A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- nucleic acid
- solid phase
- chaotrope
- process according
- solution
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
Abstract
Description
Claims
Priority Applications (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US10/509,722 US7517969B2 (en) | 2002-04-05 | 2003-04-02 | Process for isolating nucleic acid with chaotrope agents and ammonium compounds |
DE60303020.3T DE60303020T3 (en) | 2002-04-05 | 2003-04-02 | PROCESS FOR THE PURIFICATION OF NUCLEIC ACIDS WITH THE HELP OF CHAOTROPER AND AMMONIUM REAGENTS |
AT03712478T ATE314385T1 (en) | 2002-04-05 | 2003-04-02 | METHOD FOR OBTAINING NUCLEIC ACIDS USING CHAOTROPERS AND AMMONIUM REAGENTS |
EP03712478.1A EP1492804B2 (en) | 2002-04-05 | 2003-04-02 | Process for isolating nucleic acid with chaotrope agents and ammonium compounds |
JP2003582172A JP4572076B2 (en) | 2002-04-05 | 2003-04-02 | Nucleic acid isolation |
CA002481481A CA2481481A1 (en) | 2002-04-05 | 2003-04-02 | Process for isolating nucleic acid with chaotrope agents and ammonium compounds |
AU2003216572A AU2003216572A1 (en) | 2002-04-05 | 2003-04-02 | Process for isolating nucleic acid with chaotrope agents and ammonium compounds |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GBGB0207975.4A GB0207975D0 (en) | 2002-04-05 | 2002-04-05 | Isolating nucleic acid |
GB0207975.4 | 2002-04-05 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2003084976A1 true WO2003084976A1 (en) | 2003-10-16 |
Family
ID=9934375
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/IB2003/001202 WO2003084976A1 (en) | 2002-04-05 | 2003-04-02 | Process for isolating nucleic acid with chaotrope agents and ammonium compounds |
Country Status (9)
Country | Link |
---|---|
US (1) | US7517969B2 (en) |
EP (1) | EP1492804B2 (en) |
JP (1) | JP4572076B2 (en) |
AT (1) | ATE314385T1 (en) |
AU (1) | AU2003216572A1 (en) |
CA (1) | CA2481481A1 (en) |
DE (1) | DE60303020T3 (en) |
GB (1) | GB0207975D0 (en) |
WO (1) | WO2003084976A1 (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2010065420A2 (en) | 2008-12-03 | 2010-06-10 | Integrated Nano-Technologies, Inc. | Universal biological sample processing |
US8206990B2 (en) | 2006-10-10 | 2012-06-26 | Qiagen Gmbh | Methods and kit for isolating nucleic acids |
US8663918B2 (en) | 2009-05-22 | 2014-03-04 | Integrated Nano-Technologies, Inc. | Method and system for sample preparation |
US9347086B2 (en) | 2009-04-03 | 2016-05-24 | Integrated Nano-Technologies, Llc | Method and system for sample preparation |
Families Citing this family (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20060166223A1 (en) * | 2005-01-26 | 2006-07-27 | Reed Michael W | DNA purification and analysis on nanoengineered surfaces |
JP2006311803A (en) * | 2005-05-06 | 2006-11-16 | Hitachi High-Technologies Corp | Method for purifying nucleic acid and tool for purifying nucleic acid |
US8163535B2 (en) * | 2006-06-26 | 2012-04-24 | Blood Cell Storage, Inc. | Devices and processes for nucleic acid extraction |
US7608399B2 (en) * | 2006-06-26 | 2009-10-27 | Blood Cell Storage, Inc. | Device and method for extraction and analysis of nucleic acids from biological samples |
DE102007035250A1 (en) * | 2007-07-27 | 2009-01-29 | Qiagen Gmbh | Method for separating non-protein-containing biomolecules, in particular nucleic acids from protein-containing samples |
CA2742598A1 (en) * | 2008-11-04 | 2010-05-14 | Blood Cell Storage, Inc. | Nucleic acid extraction on curved glass surfaces |
DE102009022512A1 (en) * | 2009-05-25 | 2010-12-02 | Qiagen Gmbh | Process for the reactivation of silica surfaces for the isolation of nucleic acids |
WO2012159063A2 (en) | 2011-05-19 | 2012-11-22 | Blood Cell Strorage, Inc. | Gravity flow fluidic device for nucleic acid extraction |
US9416356B2 (en) * | 2013-03-15 | 2016-08-16 | Abbott Molecular Inc. | Compositions and methods for nucleic acid extraction |
CN104152436B (en) * | 2014-08-01 | 2017-12-15 | 杭州新景生物试剂开发有限公司 | DNA isolation and purification methods and its kit |
WO2016077294A1 (en) | 2014-11-14 | 2016-05-19 | Corning Incorporated | Methods and kits for post-ivt rna purification |
CA3029274A1 (en) | 2016-09-15 | 2018-03-22 | Sophie Laurenson | Devices and methods for sample analysis |
KR20230007464A (en) | 2020-06-05 | 2023-01-12 | 주식회사 씨젠 | Sample transport kit for detecting respiratory pathogens and method for detecting respiratory pathogens using the same |
WO2022132955A2 (en) * | 2020-12-16 | 2022-06-23 | Proof Diagnostics, Inc. | Coronavirus rapid diagnostics |
Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1987006621A1 (en) * | 1986-05-02 | 1987-11-05 | David Gillespie | Chaotropic method for evaluating nucleic acids in a biological sample |
EP0540170A1 (en) * | 1991-10-31 | 1993-05-05 | Becton, Dickinson and Company | Silicon tetrahydrazides and purification of DNA |
US5234809A (en) * | 1989-03-23 | 1993-08-10 | Akzo N.V. | Process for isolating nucleic acid |
EP0969090A1 (en) * | 1998-05-27 | 2000-01-05 | QIAGEN GmbH | Rapid and simple process for isolation of circular nucleic acids |
US6027945A (en) * | 1997-01-21 | 2000-02-22 | Promega Corporation | Methods of isolating biological target materials using silica magnetic particles |
EP1018549A1 (en) * | 1997-09-22 | 2000-07-12 | The Institute Of Physical & Chemical Research | Method for isolating dna |
US6090288A (en) * | 1996-02-19 | 2000-07-18 | Amersham Pharmacia Biotech Ab | Process for chromatographic separation of peptides and nucleic acid, and new high affinity ion exchange matrix |
US6270970B1 (en) * | 1999-05-14 | 2001-08-07 | Promega Corporation | Mixed-bed solid phase and its use in the isolation of nucleic acids |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0261955A3 (en) * | 1986-09-26 | 1989-06-07 | E.I. Du Pont De Nemours And Company | Process for immobilization of dna |
DE4321904B4 (en) * | 1993-07-01 | 2013-05-16 | Qiagen Gmbh | Method for chromatographic purification and separation of nucleic acid mixtures |
US5990302A (en) * | 1996-07-12 | 1999-11-23 | Toyo Boseki Kabushiki Kaisha | Method for isolating ribonucleic acid |
DE19746874A1 (en) * | 1997-10-23 | 1999-04-29 | Qiagen Gmbh | Isolation of nucleic acids |
JP4025399B2 (en) * | 1997-10-28 | 2007-12-19 | 株式会社日立製作所 | Nucleic acid recovery method and apparatus |
US6111096A (en) * | 1997-10-31 | 2000-08-29 | Bbi Bioseq, Inc. | Nucleic acid isolation and purification |
DE19943374A1 (en) * | 1999-09-10 | 2001-03-29 | Max Planck Gesellschaft | Method for binding nucleic acids to a solid phase |
EP1349951A4 (en) * | 2001-01-09 | 2005-08-24 | Whitehead Biomedical Inst | Methods and reagents for the isolation of nucleic acids |
DE10153957A1 (en) | 2001-11-06 | 2003-05-22 | Quiagen Gmbh | Process for the isolation of nucleic acids |
-
2002
- 2002-04-05 GB GBGB0207975.4A patent/GB0207975D0/en not_active Ceased
-
2003
- 2003-04-02 JP JP2003582172A patent/JP4572076B2/en not_active Expired - Lifetime
- 2003-04-02 AU AU2003216572A patent/AU2003216572A1/en not_active Abandoned
- 2003-04-02 US US10/509,722 patent/US7517969B2/en not_active Expired - Lifetime
- 2003-04-02 WO PCT/IB2003/001202 patent/WO2003084976A1/en active IP Right Grant
- 2003-04-02 EP EP03712478.1A patent/EP1492804B2/en not_active Expired - Lifetime
- 2003-04-02 DE DE60303020.3T patent/DE60303020T3/en not_active Expired - Lifetime
- 2003-04-02 CA CA002481481A patent/CA2481481A1/en not_active Abandoned
- 2003-04-02 AT AT03712478T patent/ATE314385T1/en not_active IP Right Cessation
Patent Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1987006621A1 (en) * | 1986-05-02 | 1987-11-05 | David Gillespie | Chaotropic method for evaluating nucleic acids in a biological sample |
US5234809A (en) * | 1989-03-23 | 1993-08-10 | Akzo N.V. | Process for isolating nucleic acid |
EP0540170A1 (en) * | 1991-10-31 | 1993-05-05 | Becton, Dickinson and Company | Silicon tetrahydrazides and purification of DNA |
US6090288A (en) * | 1996-02-19 | 2000-07-18 | Amersham Pharmacia Biotech Ab | Process for chromatographic separation of peptides and nucleic acid, and new high affinity ion exchange matrix |
US6027945A (en) * | 1997-01-21 | 2000-02-22 | Promega Corporation | Methods of isolating biological target materials using silica magnetic particles |
EP1018549A1 (en) * | 1997-09-22 | 2000-07-12 | The Institute Of Physical & Chemical Research | Method for isolating dna |
EP0969090A1 (en) * | 1998-05-27 | 2000-01-05 | QIAGEN GmbH | Rapid and simple process for isolation of circular nucleic acids |
US6270970B1 (en) * | 1999-05-14 | 2001-08-07 | Promega Corporation | Mixed-bed solid phase and its use in the isolation of nucleic acids |
Non-Patent Citations (3)
Title |
---|
CHRISTOPHER E. DAHLE ET AL: "Isolation of RNA from Cells in Culture Using Catrimox-14TM Cationic Surfactant", BIOTECHNIQUES, vol. 15, no. 6, 1993, pages 1102 - 1105, XP002245641 * |
HONG-KHANH NGUYEN ET AL: "Smoothing of the thermal stability of DNA duplexes by using modified nucleosides and chaotropic agents", NUCLEIC ACIDS RESEARCH, vol. 27, no. 6, 1999, pages 1492 - 1498, XP002245640 * |
STANLEY B. PRUSINER ET AL: "Thiocyanate and hydroxyl ions inactivate the scrapie agent", PROC. NATL. ACAD. SCI., vol. 78, no. 7, July 1981 (1981-07-01), pages 4606 - 4610, XP002245639 * |
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8206990B2 (en) | 2006-10-10 | 2012-06-26 | Qiagen Gmbh | Methods and kit for isolating nucleic acids |
US8460941B2 (en) | 2006-10-10 | 2013-06-11 | Qiagen Gmbh | Methods and kit for isolating nucleic acids |
WO2010065420A2 (en) | 2008-12-03 | 2010-06-10 | Integrated Nano-Technologies, Inc. | Universal biological sample processing |
EP2373795A2 (en) * | 2008-12-03 | 2011-10-12 | Integrated Nano-Technologies LLC | Universal biological sample processing |
EP2373795A4 (en) * | 2008-12-03 | 2012-10-17 | Integrated Nano Technologies Llc | Universal biological sample processing |
US9347086B2 (en) | 2009-04-03 | 2016-05-24 | Integrated Nano-Technologies, Llc | Method and system for sample preparation |
US9644200B2 (en) | 2009-04-03 | 2017-05-09 | Integrated Nano-Technologies, Llc | Method and system for sample preparation |
US10378045B2 (en) | 2009-04-03 | 2019-08-13 | Integrated Nano-Technologies, Llc | Method and system for sample preparation |
US8663918B2 (en) | 2009-05-22 | 2014-03-04 | Integrated Nano-Technologies, Inc. | Method and system for sample preparation |
Also Published As
Publication number | Publication date |
---|---|
DE60303020T2 (en) | 2006-09-28 |
CA2481481A1 (en) | 2003-10-16 |
US20050214765A1 (en) | 2005-09-29 |
JP4572076B2 (en) | 2010-10-27 |
US7517969B2 (en) | 2009-04-14 |
DE60303020T3 (en) | 2016-07-21 |
AU2003216572A1 (en) | 2003-10-20 |
GB0207975D0 (en) | 2002-05-15 |
ATE314385T1 (en) | 2006-01-15 |
DE60303020D1 (en) | 2006-02-02 |
EP1492804A1 (en) | 2005-01-05 |
JP2005525396A (en) | 2005-08-25 |
EP1492804B1 (en) | 2005-12-28 |
EP1492804B2 (en) | 2016-03-09 |
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