WO2004002214A3 - Tagetes erecta marigolds with altered carotenoid compositions and ratios - Google Patents

Tagetes erecta marigolds with altered carotenoid compositions and ratios Download PDF

Info

Publication number
WO2004002214A3
WO2004002214A3 PCT/US2003/016921 US0316921W WO2004002214A3 WO 2004002214 A3 WO2004002214 A3 WO 2004002214A3 US 0316921 W US0316921 W US 0316921W WO 2004002214 A3 WO2004002214 A3 WO 2004002214A3
Authority
WO
WIPO (PCT)
Prior art keywords
ratio
enhanced
ratios
flower petals
disclosed
Prior art date
Application number
PCT/US2003/016921
Other languages
French (fr)
Other versions
WO2004002214A2 (en
Inventor
Randal Hauptmann
Blair L Winner
Alan Blowers
Original Assignee
Ball Horticultural Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/180,775 external-priority patent/US6784351B2/en
Application filed by Ball Horticultural Co filed Critical Ball Horticultural Co
Priority to EP03761911A priority Critical patent/EP1531665A4/en
Priority to MXPA04011746A priority patent/MXPA04011746A/en
Priority to AU2003232424A priority patent/AU2003232424B2/en
Priority to CA002486116A priority patent/CA2486116A1/en
Priority to JP2004549820A priority patent/JP2006506060A/en
Publication of WO2004002214A2 publication Critical patent/WO2004002214A2/en
Publication of WO2004002214A3 publication Critical patent/WO2004002214A3/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/825Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving pigment biosynthesis
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/02Flowers
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/14Asteraceae or Compositae, e.g. safflower, sunflower, artichoke or lettuce
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/14Asteraceae or Compositae, e.g. safflower, sunflower, artichoke or lettuce
    • A01H6/1496Tagetes [marigold]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine

Abstract

A marigold plant, a regenerable portion thereof and seed are disclosed whose flower petals, leaves or flower petals and leaves contain one or more of an enhanced neoxanthin plus violaxanthin ratio, an enhanced β-carotene ratio, an enhanced lycopene ratio, an enhanced α-cryptoxanthin ratio, an enhanced phytoene ratio or an enhanced phytofluene ratio relative to that ratio in a non-mutant marigold. A marigold plant, a regenerable portion thereof and seed are also disclosed whose flower petals contain zeaxanthin esters and are substantially free of esters of both neoxanthin and violaxanthin, and wherein zeaxanthin constitutes at least about one-half of the extractable carotenoids when xanthophylls are assayed as alcohols. Also disclosed are methods of preparing such plants, oleoresins and comestible materials that have such carotenoid ratios.
PCT/US2003/016921 2002-06-26 2003-05-30 Tagetes erecta marigolds with altered carotenoid compositions and ratios WO2004002214A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP03761911A EP1531665A4 (en) 2002-06-26 2003-05-30 Tagetes erecta marigolds with altered carotenoid compositions and ratios
MXPA04011746A MXPA04011746A (en) 2002-06-26 2003-05-30 Tagetes erecta marigolds with altered carotenoid compositions and ratios.
AU2003232424A AU2003232424B2 (en) 2002-06-26 2003-05-30 Tagetes erecta marigolds with altered carotenoid compositions and ratios
CA002486116A CA2486116A1 (en) 2002-06-26 2003-05-30 Tagetes erecta marigolds with altered carotenoid compositions and ratios
JP2004549820A JP2006506060A (en) 2002-06-26 2003-05-30 African marigold with altered carotenoid composition and ratio

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US10/180,775 2002-06-26
US10/180,775 US6784351B2 (en) 2001-06-29 2002-06-26 Targetes erecta marigolds with altered carotenoid compositions and ratios
US10/325,265 2002-12-19
US10/325,265 US7033622B2 (en) 2001-06-29 2002-12-19 Tagetes erecta marigolds with altered carotenoid compositions and ratios
US10/447,769 US7575766B2 (en) 2001-06-29 2003-05-29 Tagetes erecta with altered carotenoid compositions and ratios
US10/447/769 2003-05-29

Publications (2)

Publication Number Publication Date
WO2004002214A2 WO2004002214A2 (en) 2004-01-08
WO2004002214A3 true WO2004002214A3 (en) 2004-08-05

Family

ID=32776925

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/016921 WO2004002214A2 (en) 2002-06-26 2003-05-30 Tagetes erecta marigolds with altered carotenoid compositions and ratios

Country Status (7)

Country Link
US (3) US7575766B2 (en)
EP (1) EP1531665A4 (en)
JP (1) JP2006506060A (en)
AU (1) AU2003232424B2 (en)
CA (1) CA2486116A1 (en)
MX (1) MXPA04011746A (en)
WO (1) WO2004002214A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102778436B (en) * 2011-12-20 2015-04-01 金华市农业科学研究院 Method for breeding freshly eaten corn with eye health-care effect

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6784351B2 (en) 2001-06-29 2004-08-31 Ball Horticultural Company Targetes erecta marigolds with altered carotenoid compositions and ratios
US7081478B2 (en) * 2001-06-29 2006-07-25 Chrysantis, Inc. Mixed zeaxanthin ester concentrate and uses thereof
AU2003258623A1 (en) * 2002-08-20 2004-03-11 Sungene Gmbh & Co. Kgaa Method for the production of ketocarotinoids in flower petals on plants
US8592662B2 (en) 2005-02-11 2013-11-26 Kalamazoo Holdings, Inc. Capsicum variety exhibiting a hyper-accumulation of zeaxanthin and products derived therefrom
WO2006086707A2 (en) * 2005-02-11 2006-08-17 Kalamazoo Holdings, Inc. Extracts of asteraceae containing reduced levels of phototoxic thiophene derivatives and methods for preparing same
US7595171B2 (en) * 2005-05-03 2009-09-29 Sinotrade Technology Limited Ketocarotenoids from Adonis palaestina
IL176668A0 (en) * 2006-07-02 2006-10-31 Ibr Ltd Colorless carotenoids for skin whitening
US9717265B2 (en) 2010-01-15 2017-08-01 Kemin Industries, Inc. Rumen-protected lutein product for producing high-lutein dairy products
CN103037706B (en) * 2011-01-17 2015-07-08 建明工业(珠海)有限公司 Rumen-protected lutein product for producing high-lutein dairy products
US9683240B2 (en) * 2011-10-31 2017-06-20 Philip Morris Products S.A. Modulating beta-damascenone in plants
EP2586792A1 (en) * 2011-10-31 2013-05-01 Philip Morris Products S.A. Modulating beta-damascenone in plants
WO2016073467A1 (en) * 2014-11-04 2016-05-12 Ball Horticultural Company Altered pigment deposition in tagetes patula
US9867341B2 (en) * 2014-12-16 2018-01-16 Kemin Industries, Inc. Marigold inbred line denominated KI4537

Family Cites Families (47)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2918370A (en) 1955-12-23 1959-12-22 Abbott Lab Calcium pantothenate composition
US3997679A (en) * 1974-08-12 1976-12-14 Cpc International Inc. Stabilization of xanthophylls
US4670247A (en) 1983-07-05 1987-06-02 Hoffman-Laroche Inc. Process for preparing fat-soluble vitamin active beadlets
JP3027837B2 (en) * 1988-12-27 2000-04-04 協和醗酵工業株式会社 Automatic solid sample extraction method and solid sample automatic extraction system
US5043170A (en) 1989-02-14 1991-08-27 Hoffmann-La Roche Inc. Animal feed composition containing a vitamin D metabolite
IL90794A (en) 1989-06-29 1995-03-15 Shapira Niva Nutritional sun-exposure supporting composition
US5290605A (en) 1989-06-29 1994-03-01 Niva Shapira Sun-exposure nutritional supporting composition
JP2887848B2 (en) 1989-08-30 1999-05-10 アプライド フード バイオテクノロジー、インコーポレイテッド Production of zeaxanthin and zeaxanthin-containing composition
US5618988A (en) 1990-03-02 1997-04-08 Amoco Corporation Enhanced carotenoid accumulation in storage organs of genetically engineered plants
US5684238A (en) 1990-03-02 1997-11-04 Amoco Corporation Biosynthesis of zeaxanthin and glycosylated zeaxanthin in genetically engineered hosts
GB9105420D0 (en) 1991-03-14 1991-05-01 Ici Plc Expression of genes in transgenic plants
US5270063A (en) 1992-08-03 1993-12-14 Kellogg Company Ready-to-eat cereal products enriched with beta-carotene
US5258189A (en) 1992-08-28 1993-11-02 General Mills, Inc. Method for making vitamin enriched cereal
ATE193421T1 (en) 1993-06-24 2000-06-15 Hoffmann La Roche PIGMENTATION WITH CAROTINOIDS
US6218436B1 (en) 1993-06-28 2001-04-17 The Howard Foundation Pharmaceutically active carotenoids
US6329432B2 (en) 1993-06-28 2001-12-11 The Howard Foundation Mesozeaxanthin formulations for treatment of retinal disorders
CH685189A5 (en) 1993-11-19 1995-04-28 Marigen Sa Ultramicroemulsions spontaneously dispersible concentrates with antitumoral effective xanthophyll esters.
AU702584B2 (en) 1993-12-27 1999-02-25 Kirin Holdings Kabushiki Kaisha DNA strands useful for the synthesis of xanthophylls and the process for producing the xanthophylls
US5382714A (en) * 1994-03-17 1995-01-17 The Catholic University Of America Process for isolation, purification, and recrystallization of lutein from saponified marigold oleoresin and uses thereof
US5558339A (en) * 1994-05-05 1996-09-24 Perlman; Stephen G. Network architecture to support recording and playback of real-time video games
US5523494A (en) 1994-07-20 1996-06-04 Industrial Organica, S.A. De C.V. Process for the isomerization of lutein
ATE218162T1 (en) 1994-08-23 2002-06-15 Kirin Brewery KETO GROUP INTRODUCING ENZYME, DNA CODING THEREOF AND METHOD FOR PRODUCING KETOCAROTENOID
DE69511515T2 (en) 1994-12-21 2000-01-20 Hoffmann La Roche Carotenoid ketones and esters
US5935624A (en) 1995-02-06 1999-08-10 Wisconsin Alumni Research Foundation Low phosphorus animal feed containing 1α-hydroxylated vitamin D compounds and method of preparing
AU719671B2 (en) 1995-06-07 2000-05-18 Howard Foundation, The Pharmaceutically active carotenoids
US5695794A (en) 1995-08-10 1997-12-09 Amoco Corporation Use of 25-hydroxycholecalciferol in a dietary supplement process for ameliorating the effects of tibial dyschondroplasia in poultry while maintaining weight gain
US5827652A (en) 1995-10-31 1998-10-27 Applied Food Biotechnology, Inc. Zeaxanthin formulations for human ingestion
US5747544A (en) 1995-10-31 1998-05-05 Applied Food Biotechnology, Inc. Method of using pure 3R-3'R stereoisomer of zeaxanthin to treat or prevent retinal degeneration in humans
US5854015A (en) 1995-10-31 1998-12-29 Applied Food Biotechnology, Inc. Method of making pure 3R-3'R stereoisomer of zeaxanthin for human ingestion
US5916791A (en) 1995-11-24 1999-06-29 Hirschberg; Joseph Polynucleotide molecule from Haematococcus pluvialis encoding a polypeptide having a β--C--4--oxygenase activity for biotechnological production of (3S,3S)astaxanthin
US5648564A (en) 1995-12-21 1997-07-15 Kemin Industries, Inc. Process for the formation, isolation and purification of comestible xanthophyll crystals from plants
US5858700A (en) 1997-04-03 1999-01-12 Kemin Foods, Lc Process for the isolation and purification of lycopene crystals
AU1271999A (en) * 1997-10-21 1999-05-10 Office Of Technology Liaison Process for extraction and purification of lutein, zeaxanthin and rare carotenoids from marigold flowers and plants
US6309677B1 (en) * 1998-03-24 2001-10-30 Amway Corporation Multi-carotenoid product
US6191293B1 (en) 1998-04-20 2001-02-20 Inexa, Industria Extractora C.A. Trans-xanthophyll ester concentrates of enhanced purity and methods of making same
BR9917159A (en) 1998-05-22 2002-12-17 Univ Maryland Method that uses genes to modify carotenoids for the production of ketocarotenoids carotenoid ketolase genes and products of genesis
US6224876B1 (en) 1998-08-04 2001-05-01 Biyani Milind Kesharlal Isolation and formulations of nutrient-rich carotenoids
US6056962A (en) 1998-08-04 2000-05-02 Kesharlal; Biyani Milind Isolation and formulations of nutrient-rich carotenoids
DE19838636A1 (en) * 1998-08-26 2000-03-02 Basf Ag Carotenoid formulations containing a mixture of beta-carotene, lycopene and lutein
US5955102A (en) 1998-09-04 1999-09-21 Amway Corporation Softgel capsule containing DHA and antioxidants
IL126076A (en) * 1998-09-04 2005-05-17 Ibr Ltd Transparent composition comprising phytoene and phytofluene
US6262284B1 (en) 1998-10-21 2001-07-17 University Of Maryland Process for extraction and purification of lutein, zeaxanthin and rare carotenoids from marigold flowers and plants
US6232530B1 (en) * 1998-11-30 2001-05-15 University Of Nevada Marigold DNA encoding beta-cyclase
US6254898B1 (en) 2000-05-25 2001-07-03 Protective Factors, Inc. Nutraceutical composition for protection against solar radiation
US6329557B1 (en) * 2000-06-09 2001-12-11 Prodemex, S.A. De C.V. Purification of xanthophylls from marigold extracts that contain high levels of chlorophylls
WO2002012438A2 (en) * 2000-08-04 2002-02-14 Ball Horticultural Company Marigold hybrid 50011
US7081478B2 (en) 2001-06-29 2006-07-25 Chrysantis, Inc. Mixed zeaxanthin ester concentrate and uses thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
QUACKENBUSH ET AL: "Composition and analysis of the carotenoids in marigold petals", J. ASS. OFF. ANAL., vol. 55, no. 3, 1972, pages 617 - 621, XP002965713 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102778436B (en) * 2011-12-20 2015-04-01 金华市农业科学研究院 Method for breeding freshly eaten corn with eye health-care effect

Also Published As

Publication number Publication date
AU2003232424A1 (en) 2004-01-19
US20040010826A1 (en) 2004-01-15
JP2006506060A (en) 2006-02-23
WO2004002214A2 (en) 2004-01-08
US20080125497A1 (en) 2008-05-29
US20060265784A1 (en) 2006-11-23
MXPA04011746A (en) 2005-05-16
AU2003232424B2 (en) 2008-04-24
EP1531665A4 (en) 2006-03-22
EP1531665A2 (en) 2005-05-25
US7575766B2 (en) 2009-08-18
CA2486116A1 (en) 2004-01-08

Similar Documents

Publication Publication Date Title
WO2003001901A3 (en) Tagetes erecta marigolds with altered carotenoid compositions and ratios
WO2004002214A3 (en) Tagetes erecta marigolds with altered carotenoid compositions and ratios
Cazzonelli Carotenoids in nature: insights from plants and beyond
Müller Determination of the carotenoid content in selected vegetables and fruit by HPLC and photodiode array detection
CA2070831A1 (en) Dna, constructs, cells and plants derived therefrom
RU2004106151A (en) METHOD FOR EXTRACTION OF CAROTINOIDS
Subagio et al. Stability of lutein and its myristate esters
Liaaen-Jensen Algal carotenoids and chemosystematics
ATE349917T1 (en) CAROTINOID FORMULATIONS CONTAINING A MIXTURE OF BETA-CAROTENE, LYCOPINE AND LUTEIN
WO2002099095A3 (en) Improved isoprenoid production
AU1271999A (en) Process for extraction and purification of lutein, zeaxanthin and rare carotenoids from marigold flowers and plants
WO2001018161A3 (en) Process for the preparation of conjugated linoleic acid (cla)
PL367703A1 (en) Process for the preparation of an xanthophyll
DK1229126T3 (en) Process for the preparation of carotenoid pigments
WO2014096990A8 (en) Carotene hydroxylase and its use for producing carotenoids
Valadon et al. Carotenoids in floral parts of a narcissus, a daffodil and a tulip
NO20063325L (en) New carotenoid ketolases
WO2014033703A8 (en) HUE-CONTROLLED β-CAROTENE FORMULATIONS
WO2002058683A3 (en) Carotenoids as anti-hypertension agents
Valadon et al. Carotenoids of lilies and of red pepper: biogenesis of capsanthin and capsorubin
Gerjets et al. Metabolic engineering of ketocarotenoid biosynthesis in leaves and flowers of tobacco species
Sams et al. Light quality impacts on growth, flowering, mineral uptake and petal pigmentation of marigold
WO2009022786A1 (en) Method for producing thermochromic and photochromic color flower
Katori et al. Cultivar differences in anthocyanin and carotenoid pigments in the petals of the flowering lotus (Nelumbo spp.)
CN203010279U (en) Multiband fluorescent tube and vegetative lamp

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SC SD SE SG SK SL TJ TM TN TR TT TZ UA UG UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2003232424

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 2486116

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: PA/a/2004/011746

Country of ref document: MX

WWE Wipo information: entry into national phase

Ref document number: 2004549820

Country of ref document: JP

WWE Wipo information: entry into national phase

Ref document number: 2003761911

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2003761911

Country of ref document: EP