WO2004019038A3 - Method for the detection of antibodies and/or antigens in a test liquid, particularly for determining the blood type - Google Patents

Method for the detection of antibodies and/or antigens in a test liquid, particularly for determining the blood type Download PDF

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Publication number
WO2004019038A3
WO2004019038A3 PCT/EP2003/008995 EP0308995W WO2004019038A3 WO 2004019038 A3 WO2004019038 A3 WO 2004019038A3 EP 0308995 W EP0308995 W EP 0308995W WO 2004019038 A3 WO2004019038 A3 WO 2004019038A3
Authority
WO
WIPO (PCT)
Prior art keywords
antigens
buffer
antibodies
mixture
test liquid
Prior art date
Application number
PCT/EP2003/008995
Other languages
German (de)
French (fr)
Other versions
WO2004019038A2 (en
Inventor
Joerg Spindler
Original Assignee
Deutsches Rotes Kreuz Blutspen
Joerg Spindler
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Deutsches Rotes Kreuz Blutspen, Joerg Spindler filed Critical Deutsches Rotes Kreuz Blutspen
Priority to CA2495728A priority Critical patent/CA2495728C/en
Priority to AU2003260413A priority patent/AU2003260413A1/en
Priority to EP03792313A priority patent/EP1532454A2/en
Publication of WO2004019038A2 publication Critical patent/WO2004019038A2/en
Publication of WO2004019038A3 publication Critical patent/WO2004019038A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells

Abstract

The invention relates to a method for detecting antibodies or antigens in a test liquid by means of a reaction with a given specific bonding partner. According to the inventive method, the antigens are tied to a carrier, and the resulting sample mixture is subjected to an incubation step and then centrifuged by means of a particle-buffer mixture. An agglutinate comprising the antigen that is to be detected, the carrier, and antibodies is formed in case of a positive antigen-antibody reaction. A predefined quantity of the incubated sample mixture is added to a predefined quantity of a buffer-particle mixture which is disposed inside a detection receptacle. The buffer-particle mixture is provided with an embedding buffer consisting of an aqueous solution of dextran and/or polyethylene glycol, glycine, and/or trisodium citrate.
PCT/EP2003/008995 2002-08-23 2003-08-13 Method for the detection of antibodies and/or antigens in a test liquid, particularly for determining the blood type WO2004019038A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CA2495728A CA2495728C (en) 2002-08-23 2003-08-13 Method for the detection of antibodies and/or antigens in a test liquid, particularly for determining the blood group
AU2003260413A AU2003260413A1 (en) 2002-08-23 2003-08-13 Method for the detection of antibodies and/or antigens in a test liquid, particularly for determining the blood type
EP03792313A EP1532454A2 (en) 2002-08-23 2003-08-13 Method for the detection of antibodies and/or antigens in a test liquid, particularly for determining the blood type

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10239568.3 2002-08-23
DE2002139568 DE10239568A1 (en) 2002-08-23 2002-08-23 Method for the detection of antibodies and / or antigens in a test liquid and for blood group determination

Publications (2)

Publication Number Publication Date
WO2004019038A2 WO2004019038A2 (en) 2004-03-04
WO2004019038A3 true WO2004019038A3 (en) 2004-04-22

Family

ID=31197475

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/008995 WO2004019038A2 (en) 2002-08-23 2003-08-13 Method for the detection of antibodies and/or antigens in a test liquid, particularly for determining the blood type

Country Status (5)

Country Link
EP (1) EP1532454A2 (en)
AU (1) AU2003260413A1 (en)
CA (1) CA2495728C (en)
DE (1) DE10239568A1 (en)
WO (1) WO2004019038A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102006024927B4 (en) * 2006-05-25 2008-12-11 DRK-Blutspendedienst Baden-Württemberg-Hessen gemeinnützige GmbH Method for the detection of antibodies and / or antigens and for blood group determination in a test substance
CN102445550B (en) * 2010-10-09 2014-02-05 苏州苏大赛尔免疫生物技术有限公司 ABO, RhD blood typing reagent card, preparation method thereof
CN103926415B (en) * 2014-03-24 2015-11-25 上海市血液中心 Blood group antibody associating rapid detection system in human blood
CN105628940A (en) * 2015-12-30 2016-06-01 合肥天一生物技术研究所 ABO blood subtype detection card
DE102017114537A1 (en) * 2017-06-29 2019-01-03 Endress+Hauser Conducta Gmbh+Co. Kg Sensor membrane, sensor cap and optical sensor

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5552064A (en) * 1993-02-26 1996-09-03 Ortho Diagnostic Systems, Inc. Column agglutination assay and device using biphasic centrifugation
EP0849595A1 (en) * 1996-12-18 1998-06-24 Stiftung Für Diagnostische Forschung Use of synthetic particles as reagents in agglutionation reactions
ES2126521A1 (en) * 1997-06-05 1999-03-16 Transfusion De La Comunidad Va Method for the detection of antigens present in the red corpuscle membrane, on their own or coupled, and abnormal antibodies in serum samples
US5905028A (en) * 1994-05-17 1999-05-18 Gamma Biologicals, Inc. Method and apparatus useful for detecting bloodgroup antigens and antibodies

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5552064A (en) * 1993-02-26 1996-09-03 Ortho Diagnostic Systems, Inc. Column agglutination assay and device using biphasic centrifugation
US5905028A (en) * 1994-05-17 1999-05-18 Gamma Biologicals, Inc. Method and apparatus useful for detecting bloodgroup antigens and antibodies
EP0849595A1 (en) * 1996-12-18 1998-06-24 Stiftung Für Diagnostische Forschung Use of synthetic particles as reagents in agglutionation reactions
ES2126521A1 (en) * 1997-06-05 1999-03-16 Transfusion De La Comunidad Va Method for the detection of antigens present in the red corpuscle membrane, on their own or coupled, and abnormal antibodies in serum samples

Also Published As

Publication number Publication date
CA2495728A1 (en) 2004-03-04
CA2495728C (en) 2012-08-21
AU2003260413A8 (en) 2004-03-11
AU2003260413A1 (en) 2004-03-11
EP1532454A2 (en) 2005-05-25
WO2004019038A2 (en) 2004-03-04
DE10239568A1 (en) 2004-03-04

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