WO2004020604A3 - Allele specific primer extension - Google Patents

Allele specific primer extension Download PDF

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Publication number
WO2004020604A3
WO2004020604A3 PCT/US2003/027286 US0327286W WO2004020604A3 WO 2004020604 A3 WO2004020604 A3 WO 2004020604A3 US 0327286 W US0327286 W US 0327286W WO 2004020604 A3 WO2004020604 A3 WO 2004020604A3
Authority
WO
WIPO (PCT)
Prior art keywords
specific primer
allele specific
reaction
primer extension
characterizing
Prior art date
Application number
PCT/US2003/027286
Other languages
French (fr)
Other versions
WO2004020604A2 (en
Inventor
Anup Sood
Shiv Kumar
Carl Fuller
John Nelson
John Macklin
Original Assignee
Amersham Biosciences Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amersham Biosciences Corp filed Critical Amersham Biosciences Corp
Priority to AU2003268322A priority Critical patent/AU2003268322B2/en
Priority to EP03749279A priority patent/EP1546353A4/en
Priority to CA2496462A priority patent/CA2496462C/en
Priority to JP2004532003A priority patent/JP4643263B2/en
Publication of WO2004020604A2 publication Critical patent/WO2004020604A2/en
Publication of WO2004020604A3 publication Critical patent/WO2004020604A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Abstract

A method of characterizing a nucleic acid sample is provided that includes the steps of: (a) conducting a DNA polymerase reaction that includes the reaction of a template, an allele specific primer, at least one terminal phosphate-labeled nucleotide, DNA polymerase, and optionally an enzyme having 3' ? 5' exonuclease activity when the primer is non-hydrolyzable, which reaction results in the production of labeled polyphosphate; (b) permitting the labeled polyphosphate to react with a phosphatase to produce a detectable species; (c) detecting the detectable species; and (d) characterizing the nucleic acid sample based on such detection.
PCT/US2003/027286 2002-08-29 2003-08-29 Allele specific primer extension WO2004020604A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU2003268322A AU2003268322B2 (en) 2002-08-29 2003-08-29 Allele specific primer extension
EP03749279A EP1546353A4 (en) 2002-08-29 2003-08-29 Allele specific primer extension
CA2496462A CA2496462C (en) 2002-08-29 2003-08-29 Allele specific primer extension
JP2004532003A JP4643263B2 (en) 2002-08-29 2003-08-29 Allele-specific primer extension

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US40689402P 2002-08-29 2002-08-29
US60/406,894 2002-08-29

Publications (2)

Publication Number Publication Date
WO2004020604A2 WO2004020604A2 (en) 2004-03-11
WO2004020604A3 true WO2004020604A3 (en) 2004-08-12

Family

ID=31978379

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/027286 WO2004020604A2 (en) 2002-08-29 2003-08-29 Allele specific primer extension

Country Status (5)

Country Link
EP (1) EP1546353A4 (en)
JP (1) JP4643263B2 (en)
AU (1) AU2003268322B2 (en)
CA (1) CA2496462C (en)
WO (1) WO2004020604A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9243284B2 (en) 2000-12-01 2016-01-26 Life Technologies Corporation Enzymatic nucleic acid synthesis: compositions and methods for inhibiting pyrophosphorolysis

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8507662B2 (en) 2001-01-19 2013-08-13 General Electric Company Methods and kits for reducing non-specific nucleic acid amplification
US8361712B2 (en) * 2007-12-17 2013-01-29 General Electric Company Contamination-free reagents for nucleic acid amplification
US7223541B2 (en) * 2001-08-29 2007-05-29 Ge Healthcare Bio-Sciences Corp. Terminal-phosphate-labeled nucleotides and methods of use
CA2581174A1 (en) * 2004-09-16 2006-03-30 Applera Corporation Fluorescent dye compounds, conjugates and uses thereof

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5270185A (en) * 1989-04-21 1993-12-14 Hoffmann-La Roche Inc. High-efficiency cloning of CDNA
US5518900A (en) * 1993-01-15 1996-05-21 Molecular Tool, Inc. Method for generating single-stranded DNA molecules
US5759772A (en) * 1992-07-23 1998-06-02 Wisconsin Alumni Research Foundation Method for determining the sex of an embryo
US6255083B1 (en) * 1998-12-14 2001-07-03 Li Cor Inc System and methods for nucleic acid sequencing of single molecules by polymerase synthesis
US20030134807A1 (en) * 2000-12-01 2003-07-17 Hardin Susan H. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0663447B1 (en) * 1993-12-28 2003-07-09 Eiken Chemical Co., Ltd. Method of detecting a specific polynucleotide
JP3301876B2 (en) * 1993-12-28 2002-07-15 栄研化学株式会社 Method for detecting specific polynucleotide and kit for detection used in the method
US7056661B2 (en) * 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6399335B1 (en) * 1999-11-16 2002-06-04 Advanced Research And Technology Institute, Inc. γ-phosphoester nucleoside triphosphates
WO2001094609A1 (en) * 2000-06-07 2001-12-13 Li-Cor, Inc. Charge-switch nucleotides
JP3638516B2 (en) * 2000-09-28 2005-04-13 株式会社日立製作所 Nucleic acid detection method and nucleic acid detection kit
US7052839B2 (en) * 2001-08-29 2006-05-30 Amersham Biosciences Corp Terminal-phosphate-labeled nucleotides and methods of use
US7041812B2 (en) * 2001-08-29 2006-05-09 Amersham Biosciences Corp Labeled nucleoside polyphosphates
US7033762B2 (en) * 2001-08-29 2006-04-25 Amersham Biosciences Corp Single nucleotide amplification and detection by polymerase
US6546720B2 (en) * 2001-09-04 2003-04-15 Ford Global Technologies, Inc. Method and apparatus for controlling the amount of reactant to be added to a substance using a sensor which is responsive to both the reactant and the substance

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5270185A (en) * 1989-04-21 1993-12-14 Hoffmann-La Roche Inc. High-efficiency cloning of CDNA
US5759772A (en) * 1992-07-23 1998-06-02 Wisconsin Alumni Research Foundation Method for determining the sex of an embryo
US5518900A (en) * 1993-01-15 1996-05-21 Molecular Tool, Inc. Method for generating single-stranded DNA molecules
US6255083B1 (en) * 1998-12-14 2001-07-03 Li Cor Inc System and methods for nucleic acid sequencing of single molecules by polymerase synthesis
US20030134807A1 (en) * 2000-12-01 2003-07-17 Hardin Susan H. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP1546353A4 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9243284B2 (en) 2000-12-01 2016-01-26 Life Technologies Corporation Enzymatic nucleic acid synthesis: compositions and methods for inhibiting pyrophosphorolysis

Also Published As

Publication number Publication date
AU2003268322B2 (en) 2009-07-16
JP2005537001A (en) 2005-12-08
AU2003268322A1 (en) 2004-03-19
EP1546353A2 (en) 2005-06-29
JP4643263B2 (en) 2011-03-02
CA2496462C (en) 2014-01-28
CA2496462A1 (en) 2004-03-11
WO2004020604A2 (en) 2004-03-11
EP1546353A4 (en) 2006-02-01

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