WO2004027021A3 - Device and methods for isolating target cells - Google Patents

Device and methods for isolating target cells Download PDF

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Publication number
WO2004027021A3
WO2004027021A3 PCT/US2003/021961 US0321961W WO2004027021A3 WO 2004027021 A3 WO2004027021 A3 WO 2004027021A3 US 0321961 W US0321961 W US 0321961W WO 2004027021 A3 WO2004027021 A3 WO 2004027021A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
target cells
conjugated
avid
abf
Prior art date
Application number
PCT/US2003/021961
Other languages
French (fr)
Other versions
WO2004027021A2 (en
Inventor
Steve Berstein
Amelia A Ross Ph D
Original Assignee
Nexell Of California Inc
Steve Berstein
Amelia A Ross Ph D
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nexell Of California Inc, Steve Berstein, Amelia A Ross Ph D filed Critical Nexell Of California Inc
Priority to AU2003251898A priority Critical patent/AU2003251898A1/en
Priority to EP03797812A priority patent/EP1546301A4/en
Publication of WO2004027021A2 publication Critical patent/WO2004027021A2/en
Publication of WO2004027021A3 publication Critical patent/WO2004027021A3/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/069—Absorbents; Gels to retain a fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0825—Test strips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure

Abstract

A cell isolating device and method is provided to concentrate or isolate cells with specific characteristics from a mixture of different cell types. One embodiment may comprise two subtypes of antibodies that are directly conjugated to biotin (Abb) and conjugated to a fluorescent molecule (Abf). The conjugated antibodies (Abb +Abf) bind to the target cells in a mixed cell suspension. The cell suspension is then passed over an immobilized avidin or streptavidin substrate on a glass microscope slide. The biotinylated target cells adhere to the avid in/streptavidin substrate, while the unbound cells are washed off and collected in a wicking member. Captured cells on the avid in/streptavidin substrate may then be visualized directly using a fluorescent microscope or detected and enumerated via an on-board fluorescent detection device.
PCT/US2003/021961 2002-09-18 2003-07-10 Device and methods for isolating target cells WO2004027021A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2003251898A AU2003251898A1 (en) 2002-09-18 2003-07-10 Device and methods for isolating target cells
EP03797812A EP1546301A4 (en) 2002-09-18 2003-07-10 Device and methods for isolating target cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/246,898 2002-09-18
US10/246,898 US7754155B2 (en) 2002-03-15 2002-09-18 Devices and methods for isolating target cells

Publications (2)

Publication Number Publication Date
WO2004027021A2 WO2004027021A2 (en) 2004-04-01
WO2004027021A3 true WO2004027021A3 (en) 2004-07-08

Family

ID=32028966

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/021961 WO2004027021A2 (en) 2002-09-18 2003-07-10 Device and methods for isolating target cells

Country Status (4)

Country Link
US (1) US7754155B2 (en)
EP (1) EP1546301A4 (en)
AU (1) AU2003251898A1 (en)
WO (1) WO2004027021A2 (en)

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US7553625B2 (en) * 2003-12-22 2009-06-30 John Wayne Cancer Institute Method and apparatus for in vivo collection of circulating biological components
KR100670379B1 (en) * 2005-12-15 2007-01-16 삼성에스디아이 주식회사 Organic thin film transistor, method of manufacturing the same, and organic light emitting display apparatus comprising the same
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US9879310B2 (en) * 2011-01-31 2018-01-30 The Regents Of The University Of California Nano/microscale vehicles for capture and isolation of target biomolecules and living organisms
CN104254780B (en) * 2012-02-23 2017-04-12 朱诺治疗有限公司 Chromatographic isolation of cells and other complex biological materials
CN103529202B (en) * 2013-09-13 2015-04-15 中国科学院合肥物质科学研究院 Method for reducing spontaneous mutation background of wild type human-rat hybridoma AL cells
US11274278B2 (en) 2014-04-16 2022-03-15 Juno Therapeutics Gmbh Methods, kits and apparatus for expanding a population of cells
MA45488A (en) 2015-10-22 2018-08-29 Juno Therapeutics Gmbh CELL CULTURE PROCESSES, KITS AND APPARATUS
MA45489A (en) 2015-10-22 2018-08-29 Juno Therapeutics Gmbh CELL CULTURE PROCESSES, ASSOCIATED KITS AND APPARATUS
AU2016341527B2 (en) 2015-10-22 2023-04-27 Juno Therapeutics Gmbh Methods, kits, agents and apparatuses for transduction
WO2017214550A1 (en) 2016-06-09 2017-12-14 Haimachek, Inc. Collector for detection and reversible capturing of cells from body fluids in vivo
EP3523622A1 (en) * 2016-10-06 2019-08-14 Ventana Medical Systems, Inc. System, method and kit for sample preparation
EP3615653A1 (en) 2017-04-27 2020-03-04 Juno Therapeutics GmbH Oligomeric particle reagents and methods of use thereof
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CN114002195A (en) * 2021-10-22 2022-02-01 中国科学院广州生物医药与健康研究院 System for assisting in suspended cell imaging and using method and application thereof
US11435267B1 (en) 2022-06-14 2022-09-06 Angle Europe Limited Cell recovery method and device
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Also Published As

Publication number Publication date
US20030175850A1 (en) 2003-09-18
AU2003251898A1 (en) 2004-04-08
EP1546301A4 (en) 2006-12-20
EP1546301A2 (en) 2005-06-29
WO2004027021A2 (en) 2004-04-01
US7754155B2 (en) 2010-07-13

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