WO2004033634A3 - Transgenic cells transfected with pituitary tumor transforming gene (pttg) expression vectors and uses therefor - Google Patents

Transgenic cells transfected with pituitary tumor transforming gene (pttg) expression vectors and uses therefor Download PDF

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Publication number
WO2004033634A3
WO2004033634A3 PCT/US2003/031393 US0331393W WO2004033634A3 WO 2004033634 A3 WO2004033634 A3 WO 2004033634A3 US 0331393 W US0331393 W US 0331393W WO 2004033634 A3 WO2004033634 A3 WO 2004033634A3
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WO
WIPO (PCT)
Prior art keywords
pttg
expression
cell
tsh
transgenic cells
Prior art date
Application number
PCT/US2003/031393
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French (fr)
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WO2004033634A2 (en
Inventor
Anthony P Heaney
Shlomo Melmed
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Cedars Sinai Medical Center
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Publication date
Application filed by Cedars Sinai Medical Center filed Critical Cedars Sinai Medical Center
Priority to AU2003275420A priority Critical patent/AU2003275420A1/en
Priority to JP2004543127A priority patent/JP2006501847A/en
Priority to EP03759698A priority patent/EP1546323A2/en
Publication of WO2004033634A2 publication Critical patent/WO2004033634A2/en
Publication of WO2004033634A3 publication Critical patent/WO2004033634A3/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/82—Translation products from oncogenes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0613—Cells from endocrine organs
    • C12N5/0617—Thyroid and parathyroid glands
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/998—Proteins not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Endocrinology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Oncology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Disclosed are transgenic cells trasfected with pituitary tumor transfer gene (PTTG)-­containing expression vectors and uses therefor. The nucleic acids may be operatively linked to a vector, optionally provided with control and expression sequences and/or being carried by a host cell. In one embodiment, the host cell is a thyroid stimulating hormone (TSH)-sensitive cell transfected with an expression vector comprising a DNA segment encoding functional PTTG, wherein the cell overexpresses PTTG in response to TSH. Also disclosed is an in vitro cell model for the study of genetic regulation mediated by PTTG in a mammalian cell wherein PTTG expression can be modulated by exposing the cell to TSH or estrogen. In one embodiment, the cell model is used to study the effect of PTTG expression on NIS expression or to modulate NIS expression.
PCT/US2003/031393 2002-10-04 2003-10-03 Transgenic cells transfected with pituitary tumor transforming gene (pttg) expression vectors and uses therefor WO2004033634A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU2003275420A AU2003275420A1 (en) 2002-10-04 2003-10-03 Transgenic cells transfected with pituitary tumor transforming gene (pttg) expression vectors and uses therefor
JP2004543127A JP2006501847A (en) 2002-10-04 2003-10-03 Transgenic cells transfected with pituitary tumor transforming gene (PTTG) expression vector and use thereof
EP03759698A EP1546323A2 (en) 2002-10-04 2003-10-03 Transgenic cells transfected with pituitary tumor transforming gene (pttg) expression vectors and uses therefor

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/264,372 US7097829B2 (en) 1996-11-21 2002-10-04 Transgenic cells transfected with pituitary tumor transforming gene (PTTG)) expression vectors and uses therefor
US10/264,372 2002-10-04

Publications (2)

Publication Number Publication Date
WO2004033634A2 WO2004033634A2 (en) 2004-04-22
WO2004033634A3 true WO2004033634A3 (en) 2004-07-15

Family

ID=32092350

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/031393 WO2004033634A2 (en) 2002-10-04 2003-10-03 Transgenic cells transfected with pituitary tumor transforming gene (pttg) expression vectors and uses therefor

Country Status (5)

Country Link
US (1) US7097829B2 (en)
EP (1) EP1546323A2 (en)
JP (1) JP2006501847A (en)
AU (1) AU2003275420A1 (en)
WO (1) WO2004033634A2 (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998022587A2 (en) * 1996-11-21 1998-05-28 Cedars-Sinai Medical Center Pituitary-tumor-transforming-genes, and related products

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US5831066A (en) * 1988-12-22 1998-11-03 The Trustees Of The University Of Pennsylvania Regulation of bcl-2 gene expression
WO1990009442A1 (en) 1989-02-08 1990-08-23 Uab Research Foundation Antiproliferation factor
US5500343A (en) * 1990-02-07 1996-03-19 Board Of Regents, The University Of Texas System Allelic association of the human dopamine(D2) receptor gene in compulsive disorders
AU4535893A (en) * 1992-06-15 1994-01-04 Regents Of The University Of California, The Screening assay for the identification of immunosuppressive drugs
WO1994011348A1 (en) * 1992-11-19 1994-05-26 The Trustees Of The University Of Pennsylvania Detection of hypoxia
US5684222A (en) * 1993-01-22 1997-11-04 Ontario Cancer Institute Mutant mouse having a disrupted TNFRp55
JPH08509871A (en) * 1993-09-30 1996-10-22 アグラシータス インコーポレイテッド Transgenic cotton plants producing heterologous peroxidase
US5801154A (en) * 1993-10-18 1998-09-01 Isis Pharmaceuticals, Inc. Antisense oligonucleotide modulation of multidrug resistance-associated protein
US5552390A (en) * 1993-12-09 1996-09-03 The Board Of Regents Of The University Of Nebraska Phosphorothioate inhibitors of metastatic breast cancer
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Non-Patent Citations (2)

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Also Published As

Publication number Publication date
AU2003275420A1 (en) 2004-05-04
JP2006501847A (en) 2006-01-19
WO2004033634A2 (en) 2004-04-22
US20030100530A1 (en) 2003-05-29
US7097829B2 (en) 2006-08-29
AU2003275420A8 (en) 2004-05-04
EP1546323A2 (en) 2005-06-29

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