WO2004038369A3 - Ip3 protein binding assay - Google Patents

Ip3 protein binding assay Download PDF

Info

Publication number
WO2004038369A3
WO2004038369A3 PCT/US2003/033262 US0333262W WO2004038369A3 WO 2004038369 A3 WO2004038369 A3 WO 2004038369A3 US 0333262 W US0333262 W US 0333262W WO 2004038369 A3 WO2004038369 A3 WO 2004038369A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein binding
binding assay
conjugate
reagents
detectable label
Prior art date
Application number
PCT/US2003/033262
Other languages
French (fr)
Other versions
WO2004038369A2 (en
Inventor
Tabassum Naqvi
Riaz Rouhani
Peter Fung
Richard Eglen
Rajendra Singh
Original Assignee
Discoverx Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Discoverx Inc filed Critical Discoverx Inc
Priority to CA002503228A priority Critical patent/CA2503228A1/en
Priority to AT03809590T priority patent/ATE445165T1/en
Priority to DE60329618T priority patent/DE60329618D1/en
Priority to AU2003301583A priority patent/AU2003301583A1/en
Priority to JP2004546937A priority patent/JP2006503582A/en
Priority to EP03809590A priority patent/EP1556682B8/en
Publication of WO2004038369A2 publication Critical patent/WO2004038369A2/en
Publication of WO2004038369A3 publication Critical patent/WO2004038369A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6872—Intracellular protein regulatory factors and their receptors, e.g. including ion channels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)

Abstract

Protein binding assays are provided for determining IP3 in a sample employing as reagents a conjugate of IP3 joined at the 2-oxy through a bond or linking group to a detectable label and a truncated portion of the extracellular fragment of an IP3R. The reagents are combined with the sample and the amount of IP3 determined by means of the detectable label. The conjugate with the enzyme donor fragment of β-galactosidase or a fluorescer is specifically described.
PCT/US2003/033262 2002-10-21 2003-10-20 Ip3 protein binding assay WO2004038369A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
CA002503228A CA2503228A1 (en) 2002-10-21 2003-10-20 Ip3 protein binding assay
AT03809590T ATE445165T1 (en) 2002-10-21 2003-10-20 IP3 PROTEIN BINDING TEST
DE60329618T DE60329618D1 (en) 2002-10-21 2003-10-20 IP3-PROTEIN BINDING TEST
AU2003301583A AU2003301583A1 (en) 2002-10-21 2003-10-20 Ip3 protein binding assay
JP2004546937A JP2006503582A (en) 2002-10-21 2003-10-20 IP3 protein binding assay
EP03809590A EP1556682B8 (en) 2002-10-21 2003-10-20 Ip3 protein binding assay

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US42046902P 2002-10-21 2002-10-21
US60/420,469 2002-10-21

Publications (2)

Publication Number Publication Date
WO2004038369A2 WO2004038369A2 (en) 2004-05-06
WO2004038369A3 true WO2004038369A3 (en) 2004-07-01

Family

ID=32176572

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/033262 WO2004038369A2 (en) 2002-10-21 2003-10-20 Ip3 protein binding assay

Country Status (8)

Country Link
US (1) US20040106158A1 (en)
EP (1) EP1556682B8 (en)
JP (1) JP2006503582A (en)
AT (1) ATE445165T1 (en)
AU (1) AU2003301583A1 (en)
CA (1) CA2503228A1 (en)
DE (1) DE60329618D1 (en)
WO (1) WO2004038369A2 (en)

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WO2005029039A2 (en) * 2003-09-19 2005-03-31 Echelon Biosciences Incorporated Non-radioactive inositol phosphate assay for phospholipase c-coupled drug target screening
FR2878850B1 (en) 2004-12-02 2008-10-31 Cis Bio Internat Sa DERIVATIVES OF INOSITOL-1-PHOSPHATE
US7741506B2 (en) * 2005-04-26 2010-06-22 Berry & Associates, Inc. Probe for measuring phytase activity
US20060241308A1 (en) * 2005-04-26 2006-10-26 Berry & Associates, Inc. Probe for measuring phytase activity
GB0920985D0 (en) * 2009-11-30 2010-01-13 Queen Mary & Westfield College Novel inositol phosphate derivatives
CN102410995B (en) * 2011-08-23 2013-07-31 四川汇宇制药有限公司 Screening method for IP3 receptor target medicine based on fluorescence polarization analysis
CN102286094A (en) * 2011-08-23 2011-12-21 四川汇宇制药有限公司 Truncated IP3 (inositol trisphosphate) receptor V33K-NT and application thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5977311A (en) * 1997-09-23 1999-11-02 Curagen Corporation 53BP2 complexes

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US5977311A (en) * 1997-09-23 1999-11-02 Curagen Corporation 53BP2 complexes

Also Published As

Publication number Publication date
AU2003301583A1 (en) 2004-05-13
EP1556682B8 (en) 2010-02-17
ATE445165T1 (en) 2009-10-15
US20040106158A1 (en) 2004-06-03
AU2003301583A8 (en) 2004-05-13
WO2004038369A2 (en) 2004-05-06
CA2503228A1 (en) 2004-05-06
JP2006503582A (en) 2006-02-02
EP1556682B1 (en) 2009-10-07
EP1556682A4 (en) 2007-06-06
EP1556682A2 (en) 2005-07-27
DE60329618D1 (en) 2009-11-19

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