WO2004048402B1 - A hepatitis c virus codon optimized non-structural ns3/4a fusion gene - Google Patents

A hepatitis c virus codon optimized non-structural ns3/4a fusion gene

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Publication number
WO2004048402B1
WO2004048402B1 PCT/IB2003/006361 IB0306361W WO2004048402B1 WO 2004048402 B1 WO2004048402 B1 WO 2004048402B1 IB 0306361 W IB0306361 W IB 0306361W WO 2004048402 B1 WO2004048402 B1 WO 2004048402B1
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
seq
peptide
ribavirin
composition
Prior art date
Application number
PCT/IB2003/006361
Other languages
French (fr)
Other versions
WO2004048402A2 (en
WO2004048402A3 (en
Inventor
Matti Sallberg
Original Assignee
Tripep Ab
Matti Sallberg
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tripep Ab, Matti Sallberg filed Critical Tripep Ab
Priority to ES03782748T priority Critical patent/ES2421132T3/en
Priority to CA 2506820 priority patent/CA2506820A1/en
Priority to KR1020057009575A priority patent/KR101144642B1/en
Priority to JP2004554859A priority patent/JP4660195B2/en
Priority to AU2003290385A priority patent/AU2003290385B2/en
Priority to EP03782748.2A priority patent/EP1567190B1/en
Publication of WO2004048402A2 publication Critical patent/WO2004048402A2/en
Publication of WO2004048402A3 publication Critical patent/WO2004048402A3/en
Publication of WO2004048402B1 publication Critical patent/WO2004048402B1/en
Priority to HK06102404A priority patent/HK1081973A1/en

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    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/08—RNA viruses
    • C07K14/18—Togaviridae; Flaviviridae
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    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K39/12—Viral antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/29—Hepatitis virus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
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    • C12N2770/24211—Hepacivirus, e.g. hepatitis C virus, hepatitis G virus
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Abstract

Aspects of the present invention relate to the creation of a codon-optimized hepatitis C virus (HCV) NS3/4A gene. Embodiments include said NS3/4A gene, fragments of said gene, HCV peptides encoded by said nucleic acids, nucleic acids encoding said HCV peptides, antibodies directed to said peptides, compositions containing said nucleic acids and peptides, as well as methods of making and using the aforementioned compositions including, but not limited to, diagnostics and medicaments for the treatment and prevention of HCV infection.

Claims

AMENDED CLAIMS[received by the International Bureau on 28 July 2004 (28.07.04); original claims 1-36 replaced by new claims 1-39 (3 pages) ]WHAT IS CLAIMED IS:
1. A purified or isolated nucleic acid comprising at least 30 consecutive nucleotides of the sequence of SEQ ID NO:35 or the complement thereof.
2. The purified or isolated nucleic acid of claim 1, wherein said nucleic acid comprises the sequence of SEQ ID NO:35.
3. The purified or isolated nucleic acid of claim 1, wherein said nucleic acid consists of the sequence of SEQ ID NO:35.
4. The nucleic acid of claim 1, comprising at least 50 consecutive nucleotides.
5. The nucleic acid of claim 1, comprising at least 100 consecutive nucleotides.
6. The nucleic acid of claim 1, comprising at least 200 consecutive nucleotides.
7. The nucleic acid of claim 1, comprising at least 500 consecutive nucleotides.
8. A purified or isolated nucleic acid encoding the amino acid sequence of SEQ ID NO:36.
9. A vector comprising the nucleic acid of claim 1.
10. A cell comprising the nucleic acid of claim 1.
11. A purified or isolated peptide comprising at least 30 consecutive amino acids of the sequence of SEQ ID NO:36.
12. A purified or isolated peptide comprising the amino acid sequence of SEQ ID NO:36.
13. A purified or isolated peptide consisting of the amino acid sequence of SEQ ID NO:36.
14. The peptide of claim 12 comprising at least 35 consecutive amino acids.
15. The peptide of claim 12 comprising at least 40 consecutive amino acids.
16. The peptide of claim 12 comprising at least 45 consecutive amino acids.
17. The peptide of claim 12 comprising at least 50 consecutive amino acids.
18. A pharmaceutical composition comprising the nucleic acid of claim 1, 2, 3, 4, 5, 6, 7 or 8 and a pharmaceutically acceptable carrier.
19. The pharmaceutical composition of claim 18, further comprising an adjuvant.
20. The pharmaceutical composition of claim 19, wherein said adjuvant is ribavirin.
21. A pharmaceutical composition comprising any one of the peptides of claims 11, 12, 13, 14, 15, 16 or 17.
22. The pharmaceutical composition of claim 21, further comprising an adjuvant.
23. The pharmaceutical composition of claim 22, wherein said adjuvant is ribavirin.
24. A method of making an immunogen preparation comprising: providing ribavirin; providing the nucleic acid of SEQ TD NO:35; and
77 combining said ribavirin and said nucleic acid so as to formulate said immunogen preparation.
25. A method of making an immunogen preparation comprising: providing ribavirin; providing the peptide of SEQ ID NO:36; and combining said ribavirin and said peptide so as to formulate said immunogen preparation.
26. A method of making an immunogen preparation comprising: providing ribavirin; providing the nucleic acid of SEQ ID NO:35 or a fragment thereof at least 30 consecutive nucleotides in length; and combining said ribavirin and said nucleic acid so as to formulate said immunogen preparation.
27. A method of making an immunogen preparation comprising: providing ribavirin; providing the nucleic acid of SEQ TD NO:36 or a fragment thereof at least 10 consecutive amino acids in length; and combining said ribavirin and said nucleic acid so as to formulate said immunogen preparation.
28. An improved hepatitis C virus immunogen preparation, wherein the improvement comprises a nucleic acid comprising at least 30 consecutive nucleotides of the sequence of SEQ TD NO:35.
29. The immunogen preparation of claim 28, wherein said improvement comprises a nucleic acid consisting of the sequence of SEQ TD NO:35.
30. The immunogen preparation of claim 28, wherein said improvement comprises a nucleic acid comprising the sequence of SEQ TD NO:35.
31. Use of the nucleic acid of claim 1 to prepare an HCV antiviral composition.
78
32. Use of the peptide of claim 12 to prepare an HCV antiviral composition.
33. A composition comprising the nucleic acid of claim 1.
34. A composition comprising the nucleic acid of claim 2.
35. A composition comprising the nucleic acid of claim 3.
36. A composition comprising the nucleic acid of claim 8.
37. A composition comprising the peptide of claim 11.
38. A composition comprising the nucleic acid of claim 12.
39. A composition comprising the nucleic acid of claim 13.
79
PCT/IB2003/006361 2002-11-26 2003-11-25 A hepatitis c virus codon optimized non-structural ns3/4a fusion gene WO2004048402A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
ES03782748T ES2421132T3 (en) 2002-11-26 2003-11-25 A non-structural NS3 / 4A fusion gene optimized for hepatitis C virus codon
CA 2506820 CA2506820A1 (en) 2002-11-26 2003-11-25 A hepatitis c virus codon optimized non-structural ns3/4a fusion gene
KR1020057009575A KR101144642B1 (en) 2002-11-26 2003-11-25 A hepatitis c virus codon optimized non-structural ns3/4a fusion gene
JP2004554859A JP4660195B2 (en) 2002-11-26 2003-11-25 Hepatitis C virus codon optimized nonstructural NS3 / 4A fusion gene
AU2003290385A AU2003290385B2 (en) 2002-11-26 2003-11-25 A hepatitis C virus codon optimized non-structural NS3/4A fusion gene
EP03782748.2A EP1567190B1 (en) 2002-11-26 2003-11-25 A hepatitis c virus codon optimized non-structural ns3/4a fusion gene
HK06102404A HK1081973A1 (en) 2002-11-26 2006-02-23 A hepatitis c virus codon optimized non-structural ns3/4a fusion gene

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/307,047 US7022830B2 (en) 2000-08-17 2002-11-26 Hepatitis C virus codon optimized non-structural NS3/4A fusion gene
US10/307,047 2002-11-26

Publications (3)

Publication Number Publication Date
WO2004048402A2 WO2004048402A2 (en) 2004-06-10
WO2004048402A3 WO2004048402A3 (en) 2004-07-29
WO2004048402B1 true WO2004048402B1 (en) 2004-10-07

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US (9) US7022830B2 (en)
EP (1) EP1567190B1 (en)
JP (1) JP4660195B2 (en)
KR (1) KR101144642B1 (en)
CN (1) CN100566753C (en)
AU (1) AU2003290385B2 (en)
CA (1) CA2506820A1 (en)
ES (1) ES2421132T3 (en)
HK (1) HK1081973A1 (en)
WO (1) WO2004048402A2 (en)

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US7022830B2 (en) * 2000-08-17 2006-04-04 Tripep Ab Hepatitis C virus codon optimized non-structural NS3/4A fusion gene
WO2004048403A2 (en) * 2002-11-26 2004-06-10 Tripep Ab Hepatisis c virus nonstructural protein 4a (ns4a) is an enhancer element
US7785875B2 (en) * 2004-07-03 2010-08-31 Mogam Biotechnology Research Institute Polynucleotide encoding HCV epitopes which can bind to various HLA supertypes, immunogenic composition comprising same and method of inducing an HCV-specific immune response using same
WO2008004992A2 (en) * 2005-05-25 2008-01-10 Tripep Ab Transdermal formulations containing hepatitis c virus immunogens and an ethoxylated oil
CA2618429A1 (en) * 2005-05-25 2007-03-22 Tripep Ab A hepatitis c virus non-structural ns3/4a fusion gene
PT1949913E (en) * 2005-10-07 2010-08-24 Proyecto Biomedicina Cima Sl Immunostimulatory combination for the prophylactics and treatment of hepatitis c
TW200732346A (en) * 2005-10-13 2007-09-01 Virexx Medical Corp Chimeric hepatitis C virus antigens for eliciting an immune response
JP5188400B2 (en) * 2006-03-09 2013-04-24 トランスジーン エス．エー． Nonstructural fusion protein of hepatitis C virus
EP2185195A2 (en) 2007-08-16 2010-05-19 Tripep Ab Immunogen platform
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