WO2004081566A3 - Method and device for detecting biomolecules - Google Patents

Method and device for detecting biomolecules Download PDF

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Publication number
WO2004081566A3
WO2004081566A3 PCT/EP2004/002685 EP2004002685W WO2004081566A3 WO 2004081566 A3 WO2004081566 A3 WO 2004081566A3 EP 2004002685 W EP2004002685 W EP 2004002685W WO 2004081566 A3 WO2004081566 A3 WO 2004081566A3
Authority
WO
WIPO (PCT)
Prior art keywords
nanoelectrodes
biomolecules
tag
segment
probe molecules
Prior art date
Application number
PCT/EP2004/002685
Other languages
French (fr)
Other versions
WO2004081566A2 (en
Inventor
Bernard Mandrand
Agnes Dupont-Filliard
Original Assignee
Apibio Sas
Bernard Mandrand
Agnes Dupont-Filliard
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Apibio Sas, Bernard Mandrand, Agnes Dupont-Filliard filed Critical Apibio Sas
Priority to DE602004011028T priority Critical patent/DE602004011028T2/en
Priority to US10/548,869 priority patent/US7879594B2/en
Priority to EP04720593A priority patent/EP1604042B1/en
Priority to JP2006504693A priority patent/JP4625547B2/en
Publication of WO2004081566A2 publication Critical patent/WO2004081566A2/en
Publication of WO2004081566A3 publication Critical patent/WO2004081566A3/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/125—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being enzymatic, i.e. proteins, and non proteins, such as nucleic acid with enzymatic activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/113—Detection mode being characterised by the assay principle based on agglutination/precipitation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/607—Detection means characterised by use of a special device being a sensor, e.g. electrode
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/70—Nanostructure
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/70—Nanostructure
    • Y10S977/701—Integrated with dissimilar structures on a common substrate
    • Y10S977/702—Integrated with dissimilar structures on a common substrate having biological material component
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/70—Nanostructure
    • Y10S977/701—Integrated with dissimilar structures on a common substrate
    • Y10S977/702—Integrated with dissimilar structures on a common substrate having biological material component
    • Y10S977/704—Nucleic acids, e.g. DNA or RNA
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/70—Nanostructure
    • Y10S977/701—Integrated with dissimilar structures on a common substrate
    • Y10S977/702—Integrated with dissimilar structures on a common substrate having biological material component
    • Y10S977/705—Protein or peptide
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/70—Nanostructure
    • Y10S977/701—Integrated with dissimilar structures on a common substrate
    • Y10S977/72—On an electrically conducting, semi-conducting, or semi-insulating substrate

Abstract

The present invention relates to a method for detecting a target biomolecule in a sample comprising a plurality of biomolecules, whereby the target biomolecule is provided with a tag, said tag comprising a catalytic active moiety which catalyses a reaction yielding an insoluble reaction product which precipitates on flexible electrically conductive nanoelectrodes. The precipitation onto said nanoelectrodes causes a change in their electroconductivity which is accessible to electroanalytical methods. The invention relates further to a a biochip comprising a solid support with nanoelectrodes attached thereto and probe molecules bound to all or to a plurality of said nanoelectrodes which may be the same or different, a segment of said probe molecules being able to interact specifically with a segment of the target biomolecules.
PCT/EP2004/002685 2003-03-14 2004-03-15 Method and device for detecting biomolecules WO2004081566A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
DE602004011028T DE602004011028T2 (en) 2003-03-14 2004-03-15 METHOD AND DEVICE FOR DETECTING BIOMOLECULES
US10/548,869 US7879594B2 (en) 2003-03-14 2004-03-15 Method and device for detecting biomolecules
EP04720593A EP1604042B1 (en) 2003-03-14 2004-03-15 Method and device for detecting biomolecules
JP2006504693A JP4625547B2 (en) 2003-03-14 2004-03-15 Methods and apparatus for biomolecule detection

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10311315A DE10311315A1 (en) 2003-03-14 2003-03-14 Method and device for the detection of biomolecules
DE10311315.0 2003-03-14

Publications (2)

Publication Number Publication Date
WO2004081566A2 WO2004081566A2 (en) 2004-09-23
WO2004081566A3 true WO2004081566A3 (en) 2004-12-02

Family

ID=32920804

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2004/002685 WO2004081566A2 (en) 2003-03-14 2004-03-15 Method and device for detecting biomolecules

Country Status (7)

Country Link
US (1) US7879594B2 (en)
EP (1) EP1604042B1 (en)
JP (1) JP4625547B2 (en)
AT (1) ATE382713T1 (en)
DE (2) DE10311315A1 (en)
ES (1) ES2298730T3 (en)
WO (1) WO2004081566A2 (en)

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DE102004024364A1 (en) * 2004-05-17 2005-12-15 Apibio Sas Process for the preparation of polymers
US20070231794A1 (en) * 2005-09-21 2007-10-04 Combimatrix Corporation Process to detect binding events on an electrode microarray using enzymes
DE102007055386B4 (en) * 2007-11-20 2015-07-16 Boehringer Ingelheim Vetmedica Gmbh Method for calibrating a sensor element
EP2803988B1 (en) * 2008-09-02 2017-04-12 The Governing Council Of The University Of Toronto Nanostructured microelectrodes and biosensing devices incorporating the same
US8945943B2 (en) 2010-05-26 2015-02-03 The Board Of Trustees Of The University Of Illinois Personal glucose meters for detection and quantification of a broad range of analytes
US8877022B2 (en) * 2011-06-21 2014-11-04 National Taiwan University Biosensor
GB201120118D0 (en) 2011-11-22 2012-01-04 Iti Scotland Ltd Detecting analytes
CN103278621A (en) * 2013-04-25 2013-09-04 中国科学院广州生物医药与健康研究院 Method for detecting transcription factor by biosensor and glucometer
CN103558396B (en) * 2013-10-29 2015-08-26 宁波大学 A kind of quantitative detecting method of alpha-fetoprotein
FR3034523B1 (en) * 2015-04-03 2020-01-17 Universite De Bourgogne NOVEL METHOD FOR DETECTING THE PRESENCE OF BACTERIA PRODUCING BETA-LACTAMASES.
KR101701618B1 (en) * 2015-06-23 2017-02-13 국립암센터 Nanostructure for Detecting cell-free DNA using Conductive Polymer and the Use thereof
ZA201607865B (en) 2015-11-18 2019-10-30 Univ Stellenbosch A device for detecting target biomolecules
CN115856046B (en) * 2023-01-09 2023-05-26 中南大学 Application of photoelectrochemical biosensor to detection of ALP (ALP) based on perovskite nano material

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Also Published As

Publication number Publication date
DE10311315A1 (en) 2004-09-30
ES2298730T3 (en) 2008-05-16
US20070037153A1 (en) 2007-02-15
JP4625547B2 (en) 2011-02-02
US7879594B2 (en) 2011-02-01
DE602004011028T2 (en) 2009-01-02
JP2006520469A (en) 2006-09-07
WO2004081566A2 (en) 2004-09-23
EP1604042B1 (en) 2008-01-02
ATE382713T1 (en) 2008-01-15
DE602004011028D1 (en) 2008-02-14
EP1604042A2 (en) 2005-12-14

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