WO2004090096A3 - Methods for neural differentiation of embryonic stem cells using protease passaging techniques - Google Patents

Methods for neural differentiation of embryonic stem cells using protease passaging techniques Download PDF

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Publication number
WO2004090096A3
WO2004090096A3 PCT/US2004/010121 US2004010121W WO2004090096A3 WO 2004090096 A3 WO2004090096 A3 WO 2004090096A3 US 2004010121 W US2004010121 W US 2004010121W WO 2004090096 A3 WO2004090096 A3 WO 2004090096A3
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WO
WIPO (PCT)
Prior art keywords
methods
cells
human
stem cells
embryonic stem
Prior art date
Application number
PCT/US2004/010121
Other languages
French (fr)
Other versions
WO2004090096A2 (en
Inventor
Thomas C Schulz
Brian Condie
Allan Robins
Original Assignee
Bresagen Inc
Univ Georgia Res Found
Thomas C Schulz
Brian Condie
Allan Robins
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bresagen Inc, Univ Georgia Res Found, Thomas C Schulz, Brian Condie, Allan Robins filed Critical Bresagen Inc
Priority to EP04758764A priority Critical patent/EP1615997A4/en
Priority to AU2004227366A priority patent/AU2004227366B2/en
Priority to US10/551,603 priority patent/US20060183221A1/en
Priority to CA002521039A priority patent/CA2521039A1/en
Publication of WO2004090096A2 publication Critical patent/WO2004090096A2/en
Publication of WO2004090096A3 publication Critical patent/WO2004090096A3/en
Priority to AU2009201414A priority patent/AU2009201414A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0619—Neurons
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/90—Polysaccharides
    • C12N2501/91—Heparin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells

Abstract

The present invention provides methods for human pluripotent cell culturing and for neural cell production. More particularly, the present invention provides culturing methods employing dissociating cell cultures to an essentially single cell culture, such as by employing antibody selection and bulk passaging treatments utilizing the subsequent application of Collagenase and trypsin. In certain embodiments, the cells are further treated with essentially serum free MEDII conditioned medium, proline, or minimal medium, and are optionally treated with amphiphilic lipid compounds for the generation of human neural cells from pluripotent human cells. In certain embodiments, the cells cultured using these methods have an abnormal karyotype.
PCT/US2004/010121 2002-08-08 2004-03-31 Methods for neural differentiation of embryonic stem cells using protease passaging techniques WO2004090096A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP04758764A EP1615997A4 (en) 2002-08-08 2004-03-31 Methods for neural differentiation of embryonic stem cells using protease passaging techniques
AU2004227366A AU2004227366B2 (en) 2002-08-08 2004-03-31 Methods for neural differentiation of embryonic stem cells using protease passaging techniques
US10/551,603 US20060183221A1 (en) 2003-03-31 2004-03-31 Method for neural differentiation of embryonic stem cells using protease passaging techniques
CA002521039A CA2521039A1 (en) 2002-08-08 2004-03-31 Methods for neural differentiation of embryonic stem cells using protease passaging techniques
AU2009201414A AU2009201414A1 (en) 2002-08-08 2009-04-09 Methods for neural differentiation of embryonic stem cells using protease passaging techniques

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US40196802P 2002-08-08 2002-08-08
US45909003P 2003-03-31 2003-03-31
US60/459,090 2003-03-31

Publications (2)

Publication Number Publication Date
WO2004090096A2 WO2004090096A2 (en) 2004-10-21
WO2004090096A3 true WO2004090096A3 (en) 2005-03-03

Family

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Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2003/024864 WO2004015077A2 (en) 2002-08-08 2003-08-08 Compositions and methods for neural differentiation of embryonic stem cells
PCT/US2004/010121 WO2004090096A2 (en) 2002-08-08 2004-03-31 Methods for neural differentiation of embryonic stem cells using protease passaging techniques

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/US2003/024864 WO2004015077A2 (en) 2002-08-08 2003-08-08 Compositions and methods for neural differentiation of embryonic stem cells

Country Status (5)

Country Link
US (1) US20060121607A1 (en)
EP (2) EP1534068A4 (en)
AU (2) AU2004227366B2 (en)
CA (1) CA2521039A1 (en)
WO (2) WO2004015077A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7105916B2 (en) 2018-04-23 2022-07-25 エンジーケム ライフサイエンシーズ コーポレイション Glycerol derivative, method for producing same, and immunomodulator containing same as active ingredient

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US7927587B2 (en) 1999-08-05 2011-04-19 Regents Of The University Of Minnesota MAPC administration for the treatment of lysosomal storage disorders
US8153424B2 (en) 2001-10-03 2012-04-10 Wisconsin Alumni Research Foundation Method of in vitro differentiation of neural stem cells, motor neurons and dopamine neurons from primate embryonic stem cells
US7588937B2 (en) 2001-10-03 2009-09-15 Wisconsin Alumni Research Foundation Method of in vitro differentiation of neural stem cells, motor neurons and dopamine neurons from primate embryonic stem cells
RU2345133C2 (en) 2003-03-12 2009-01-27 Релайанс Лайф Сайенсиз Пвт. Лтд. Production of terminal-differentiated dofaminnergic neurones from embrionic human founder cells
US7820439B2 (en) 2003-09-03 2010-10-26 Reliance Life Sciences Pvt Ltd. In vitro generation of GABAergic neurons from pluripotent stem cells
ES2579804T3 (en) * 2003-12-02 2016-08-16 Celavie Biosciences, Llc Compositions and methods for the propagation of neural progenitor cells
EP1694345A1 (en) 2003-12-04 2006-08-30 Regents Of The University Of Minnesota Compositions and methods for the treatment of lysosomal storage disorders
EP1743012A4 (en) * 2004-04-09 2008-08-27 Ronald S Coldstein Methods for generating neuronal cells from human embryonic stem cells and uses thereof
JP5141016B2 (en) * 2004-06-18 2013-02-13 独立行政法人理化学研究所 Induction of neural differentiation of embryonic stem cells by serum-free suspension culture
WO2006084314A1 (en) * 2005-02-09 2006-08-17 Australian Stem Cell Centre Limited Stem cell populations and classification system
CN101410509B (en) 2006-02-23 2016-05-18 维亚赛特公司 For cultivate can noble cells composition and method
US20100135970A1 (en) * 2006-10-27 2010-06-03 Caritas St. Elizabeth Medical Center Of Boston, In Methods for Reprogramming Adult Somatic Cells and Uses Thereof
US9005964B2 (en) 2006-11-24 2015-04-14 Regents Of The University Of Minnesota Endodermal progenitor cells
MY157964A (en) * 2006-12-19 2016-08-30 Stempeutics Res Private Ltd Germ lineage derived feeder cells and methods thereof
US8008075B2 (en) 2008-11-04 2011-08-30 Viacyte, Inc. Stem cell aggregate suspension compositions and methods of differentiation thereof
US8895300B2 (en) 2008-11-04 2014-11-25 Viacyte, Inc. Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof
WO2012135621A2 (en) * 2011-03-30 2012-10-04 Cellular Dynamics International. Inc Priming of pluripotent stem cells for neural differentiation
WO2016032151A1 (en) * 2014-08-25 2016-03-03 한국생명공학연구원 Method for producing cholinergic neural cells
KR101793722B1 (en) * 2014-08-25 2017-11-03 한국생명공학연구원 Process for preparing astrocyte
US11566221B2 (en) 2016-10-24 2023-01-31 Life Technologies Corporation Methods of differentiation to neuronal cells and kits therefor
US20190167724A1 (en) * 2017-12-04 2019-06-06 Caire Medical-Biotechnology International Co. Neuroprotective composition, preparation process thereof and medical uses thereof
CN111484977B (en) * 2019-01-25 2023-05-16 中国科学院脑科学与智能技术卓越创新中心 Method of reprogramming to produce functional noradrenergic neurons
CN114657119B (en) * 2022-03-16 2023-10-20 广东省农业科学院动物科学研究所 Separation and primary culture method of primary piglet intestinal glial cells

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US20030166272A1 (en) * 1995-02-02 2003-09-04 Abuljadayel Ilham Saleh Method of preparing an undifferentiated cell

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Publication number Priority date Publication date Assignee Title
JP7105916B2 (en) 2018-04-23 2022-07-25 エンジーケム ライフサイエンシーズ コーポレイション Glycerol derivative, method for producing same, and immunomodulator containing same as active ingredient

Also Published As

Publication number Publication date
EP1534068A2 (en) 2005-06-01
US20060121607A1 (en) 2006-06-08
EP1615997A2 (en) 2006-01-18
WO2004015077A2 (en) 2004-02-19
AU2009201414A1 (en) 2009-05-07
EP1534068A4 (en) 2006-08-23
AU2004227366B2 (en) 2009-01-08
EP1615997A4 (en) 2007-08-29
AU2004227366A1 (en) 2004-10-21
CA2521039A1 (en) 2004-10-21
WO2004015077A3 (en) 2004-05-13
WO2004090096A2 (en) 2004-10-21
WO2004015077A9 (en) 2004-06-17

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