WO2004094611A3 - Reprogramming nuclear function with somatic cell cytoplasm - Google Patents

Reprogramming nuclear function with somatic cell cytoplasm Download PDF

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Publication number
WO2004094611A3
WO2004094611A3 PCT/US2004/012494 US2004012494W WO2004094611A3 WO 2004094611 A3 WO2004094611 A3 WO 2004094611A3 US 2004012494 W US2004012494 W US 2004012494W WO 2004094611 A3 WO2004094611 A3 WO 2004094611A3
Authority
WO
WIPO (PCT)
Prior art keywords
reprogramming
somatic cell
cell cytoplasm
nuclear function
cell
Prior art date
Application number
PCT/US2004/012494
Other languages
French (fr)
Other versions
WO2004094611A2 (en
Inventor
James M Robl
Original Assignee
Univ Massachusetts
James M Robl
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2004/001375 external-priority patent/WO2005049788A2/en
Application filed by Univ Massachusetts, James M Robl filed Critical Univ Massachusetts
Priority to AU2004232917A priority Critical patent/AU2004232917A1/en
Priority to CA002527041A priority patent/CA2527041A1/en
Priority to EP04760140A priority patent/EP1620546A2/en
Publication of WO2004094611A2 publication Critical patent/WO2004094611A2/en
Priority to IL172145A priority patent/IL172145A0/en
Publication of WO2004094611A3 publication Critical patent/WO2004094611A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/70—Undefined extracts
    • C12N2500/80—Undefined extracts from animals
    • C12N2500/84—Undefined extracts from animals from mammals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/11—Coculture with; Conditioned medium produced by blood or immune system cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2517/00—Cells related to new breeds of animals
    • C12N2517/10—Conditioning of cells for in vitro fecondation or nuclear transfer

Abstract

The invention provides methods for altering the expression profile of a cell to convert the cell from one type to a desired cell type. These reprogrammed cells may be used in a variety of medical applications for treating a mammal in need of a particular cell type.
PCT/US2004/012494 2003-04-21 2004-04-21 Reprogramming nuclear function with somatic cell cytoplasm WO2004094611A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU2004232917A AU2004232917A1 (en) 2003-04-21 2004-04-21 Reprogramming nuclear function with somatic cell cytoplasm
CA002527041A CA2527041A1 (en) 2003-04-21 2004-04-21 Reprogramming nuclear function with somatic cell cytoplasm
EP04760140A EP1620546A2 (en) 2003-04-21 2004-04-21 Reprogramming nuclear function with somatic cell cytoplasm
IL172145A IL172145A0 (en) 2003-04-21 2005-11-23 Reprogramming nuclear function with somatic cell cytoplasm

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US46422703P 2003-04-21 2003-04-21
US60/464,227 2003-04-21
USPCT/US04/001375 2004-01-20
PCT/US2004/001375 WO2005049788A2 (en) 2003-01-17 2004-01-20 Reprogramming of somatic cell nuclei

Publications (2)

Publication Number Publication Date
WO2004094611A2 WO2004094611A2 (en) 2004-11-04
WO2004094611A3 true WO2004094611A3 (en) 2007-01-25

Family

ID=35530791

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/012494 WO2004094611A2 (en) 2003-04-21 2004-04-21 Reprogramming nuclear function with somatic cell cytoplasm

Country Status (5)

Country Link
EP (1) EP1620546A2 (en)
AU (1) AU2004232917A1 (en)
CA (1) CA2527041A1 (en)
IL (1) IL172145A0 (en)
WO (1) WO2004094611A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020142397A1 (en) 2000-12-22 2002-10-03 Philippe Collas Methods for altering cell fate
US20080112937A1 (en) * 2004-07-22 2008-05-15 Lewis T Williams Method of Producing Autologous Embryonic Stem Cells

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6211429B1 (en) * 1997-06-18 2001-04-03 The Curators Of The University Of Missouri Complete oocyte activation using an oocyte-modifying agent and a reducing agent
US20020001842A1 (en) * 1999-06-30 2002-01-03 Chapman Karen B. Cytoplasmic transfer to de-differentiate recipient cells
US20020142397A1 (en) * 2000-12-22 2002-10-03 Philippe Collas Methods for altering cell fate
US20040068760A1 (en) * 1999-11-19 2004-04-08 Robl James M. Transgenic ungulates capable of human antibody production

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6211429B1 (en) * 1997-06-18 2001-04-03 The Curators Of The University Of Missouri Complete oocyte activation using an oocyte-modifying agent and a reducing agent
US20020001842A1 (en) * 1999-06-30 2002-01-03 Chapman Karen B. Cytoplasmic transfer to de-differentiate recipient cells
US20040068760A1 (en) * 1999-11-19 2004-04-08 Robl James M. Transgenic ungulates capable of human antibody production
US20020142397A1 (en) * 2000-12-22 2002-10-03 Philippe Collas Methods for altering cell fate

Also Published As

Publication number Publication date
CA2527041A1 (en) 2004-11-04
WO2004094611A2 (en) 2004-11-04
IL172145A0 (en) 2009-02-11
AU2004232917A1 (en) 2004-11-04
EP1620546A2 (en) 2006-02-01

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