WO2004111218A3 - Method for producing glucoamylases and their uses - Google Patents

Method for producing glucoamylases and their uses Download PDF

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Publication number
WO2004111218A3
WO2004111218A3 PCT/DK2004/000406 DK2004000406W WO2004111218A3 WO 2004111218 A3 WO2004111218 A3 WO 2004111218A3 DK 2004000406 W DK2004000406 W DK 2004000406W WO 2004111218 A3 WO2004111218 A3 WO 2004111218A3
Authority
WO
WIPO (PCT)
Prior art keywords
producing
polypeptides
glucoamylases
nucleic acid
methods
Prior art date
Application number
PCT/DK2004/000406
Other languages
French (fr)
Other versions
WO2004111218A2 (en
Inventor
Anders Viksoe-Nielsen
Barrie Edmund Norman
Sara Landvik
Original Assignee
Novozymes As
Anders Viksoe-Nielsen
Barrie Edmund Norman
Sara Landvik
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As, Anders Viksoe-Nielsen, Barrie Edmund Norman, Sara Landvik filed Critical Novozymes As
Priority to US10/578,992 priority Critical patent/US20060270007A1/en
Priority to EP04736629A priority patent/EP1633874A2/en
Publication of WO2004111218A2 publication Critical patent/WO2004111218A2/en
Publication of WO2004111218A3 publication Critical patent/WO2004111218A3/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • AHUMAN NECESSITIES
    • A21BAKING; EDIBLE DOUGHS
    • A21DTREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
    • A21D8/00Methods for preparing or baking dough
    • A21D8/02Methods for preparing dough; Treating dough prior to baking
    • A21D8/04Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes
    • A21D8/042Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes with enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/30Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
    • A23L29/35Degradation products of starch, e.g. hydrolysates, dextrins; Enzymatically modified starches
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17Amino acids, peptides or proteins
    • A23L33/18Peptides; Protein hydrolysates
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/20Removal of unwanted matter, e.g. deodorisation or detoxification
    • A23L5/25Removal of unwanted matter, e.g. deodorisation or detoxification using enzymes
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • A61K38/16Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46Hydrolases (3)
    • A61K38/47Hydrolases (3) acting on glycosyl compounds (3.2), e.g. cellulases, lactases
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16Organic compounds
    • C11D3/38Products with no well-defined composition, e.g. natural products
    • C11D3/386Preparations containing enzymes, e.g. protease or amylase
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16Organic compounds
    • C11D3/38Products with no well-defined composition, e.g. natural products
    • C11D3/386Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38636Preparations containing enzymes, e.g. protease or amylase containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12CBEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C5/00Other raw materials for the preparation of beer
    • C12C5/004Enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12GWINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
    • C12G1/00Preparation of wine or sparkling wine
    • C12G1/02Preparation of must from grapes; Must treatment and fermentation
    • C12G1/0203Preparation of must from grapes; Must treatment and fermentation by microbiological or enzymatic treatment
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2428Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/14Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Polymers & Plastics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to methods for producing isolated polypeptides having glucoamylase activity, filamentous fungal host cells comprising nucleic acid sequences encoding the polypeptides, and using the polypeptides.
PCT/DK2004/000406 2003-06-13 2004-06-11 Method for producing glucoamylases and their uses WO2004111218A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US10/578,992 US20060270007A1 (en) 2003-06-13 2004-06-11 Method for producing glucoamylases and their uses
EP04736629A EP1633874A2 (en) 2003-06-13 2004-06-11 Method for producing glucoamylases and their uses

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200300884 2003-06-13
DKPA200300884 2003-06-13

Publications (2)

Publication Number Publication Date
WO2004111218A2 WO2004111218A2 (en) 2004-12-23
WO2004111218A3 true WO2004111218A3 (en) 2005-02-24

Family

ID=33547532

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2004/000406 WO2004111218A2 (en) 2003-06-13 2004-06-11 Method for producing glucoamylases and their uses

Country Status (4)

Country Link
US (1) US20060270007A1 (en)
EP (1) EP1633874A2 (en)
CN (1) CN1816627A (en)
WO (1) WO2004111218A2 (en)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080113418A1 (en) 2004-01-16 2008-05-15 Novozymes A/S Processes for Producing a Fermantation Product
US7332319B2 (en) 2004-05-27 2008-02-19 Genencor International, Inc. Heterologous alpha amylase expression in Aspergillus
EP2365068B1 (en) 2004-12-22 2017-03-01 Novozymes A/S Enzymes for starch processing
US8841091B2 (en) 2004-12-22 2014-09-23 Novozymes Als Enzymes for starch processing
EP1831362B1 (en) 2004-12-30 2011-10-26 Genencor International, Inc. Acid fungal proteases
MX2007014229A (en) * 2005-05-19 2008-02-07 Novozymes North America Inc Production of enzymes.
MX2008013100A (en) 2006-04-19 2008-10-27 Novozymes North America Inc Polypeptides having glucoamylase activity and polynucleotides encoding same.
US7968318B2 (en) 2006-06-06 2011-06-28 Genencor International, Inc. Process for conversion of granular starch to ethanol
US20100048880A1 (en) * 2006-10-31 2010-02-25 Simpson Biotech Co., Ltd. Starch Binding Domain and Use Thereof
US8399224B2 (en) 2007-03-14 2013-03-19 Danisco Us Inc. Production of ethanol from barley and DDGS containing reduced beta-glucan and phytic acid
EP2554666B1 (en) * 2008-01-02 2015-08-05 Danisco US Inc. A process of obtaining ethanol without glucoamylase using pseudomonas saccharophila G4-amylase and variants thereof
EP2271765A1 (en) 2008-03-11 2011-01-12 Danisco US Inc. Use of rhizopus amylases in granular starch hydrolysis
MX2010009652A (en) 2008-03-11 2010-09-30 Danisco Us Inc Glucoamylase and buttiauxiella phytase during saccharification.
US8986955B2 (en) * 2009-05-15 2015-03-24 Simpson Biotech Co., Ltd. Method for increasing thermal stability and retaining activity of a protein
CA2782036C (en) * 2009-12-01 2019-01-15 Novozymes A/S Polypeptides having glucoamylase activity and polynucleotides encoding same
US9617527B2 (en) 2010-04-14 2017-04-11 Novozymes A/S Polypeptides having glucoamylase activity and polynucleotides encoding same
CN102939386B (en) * 2010-04-14 2016-04-13 诺维信公司 Produce the method for tunning
MX338068B (en) * 2010-04-14 2016-04-01 Novozymes As Polypeptides having glucoamylase activity and polynucleotides encoding same.
EP2787070A3 (en) * 2011-07-21 2015-03-11 AB Enzymes GmbH Process of lysing yeast cell walls
CA2846690A1 (en) 2011-08-26 2013-03-07 Novozymes A/S Polypeptides having glucoamylase activity and polynucleotides encoding same
US9994834B2 (en) 2011-09-09 2018-06-12 Novozymes A/S Polynucleotides encoding polypeptides having alpha-amylase activity and methods of making the same
EP2761013A1 (en) 2011-09-29 2014-08-06 Danisco US Inc. Liquefaction and saccharification of granular starch at high concentration
CA3037875A1 (en) 2016-09-23 2018-03-29 Dupont Nutrition Biosciences Aps Use of low ph active alpha-1,4/1,6-glycoside hydrolases as a feed additive for ruminants to enhance starch digestion

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3912590A (en) * 1973-01-03 1975-10-14 Novo Industri As Procedure for liquefying starch
US6558920B1 (en) * 1999-06-01 2003-05-06 Gekkeikan Sake Company Limited High expression system of proteins

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62126989A (en) * 1985-11-26 1987-06-09 Godo Shiyusei Kk Method for saccharifying starch by using enzyme produced by basidiomycetes belonging to genus corticium without steaming or boiling

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3912590A (en) * 1973-01-03 1975-10-14 Novo Industri As Procedure for liquefying starch
US6558920B1 (en) * 1999-06-01 2003-05-06 Gekkeikan Sake Company Limited High expression system of proteins

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL [online] 10 February 1999 (1999-02-10), NAGASAKA Y ET AL: "Corticium rolfsii mRNA for glucoamylase G2, complete cds.", XP002269424, Database accession no. D49448 *
NAGASAKA Y ET AL: "Cloning of Corticium rolfsii glucoamylase cDNA and its expression in Saccharomyces cerevisiae", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 44, no. 3-4, 1995, pages 451 - 458, XP002269423, ISSN: 0175-7598 *
NAGASAKA, YOSUKE ET AL: "Study of glucoamylase from Corticium rolfsii", JOURNAL OF APPLIED GLYCOSCIENCE, vol. 46, no. 2, 1999, pages 169 - 178, XP008027339 *

Also Published As

Publication number Publication date
US20060270007A1 (en) 2006-11-30
EP1633874A2 (en) 2006-03-15
WO2004111218A2 (en) 2004-12-23
CN1816627A (en) 2006-08-09

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