WO2005003332A3 - Diamagnetically stabilised three-dimensional cell culture - Google Patents

Diamagnetically stabilised three-dimensional cell culture Download PDF

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Publication number
WO2005003332A3
WO2005003332A3 PCT/US2004/020908 US2004020908W WO2005003332A3 WO 2005003332 A3 WO2005003332 A3 WO 2005003332A3 US 2004020908 W US2004020908 W US 2004020908W WO 2005003332 A3 WO2005003332 A3 WO 2005003332A3
Authority
WO
WIPO (PCT)
Prior art keywords
diamagnetically
stabilised
cell culture
dimensional cell
tissue
Prior art date
Application number
PCT/US2004/020908
Other languages
French (fr)
Other versions
WO2005003332A2 (en
Inventor
Jeanne L Becker
Susan B Coffin
Original Assignee
Univ South Florida
Jeanne L Becker
Susan B Coffin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ South Florida, Jeanne L Becker, Susan B Coffin filed Critical Univ South Florida
Publication of WO2005003332A2 publication Critical patent/WO2005003332A2/en
Publication of WO2005003332A3 publication Critical patent/WO2005003332A3/en
Priority to US12/330,071 priority Critical patent/US20090137018A1/en
Priority to US14/096,791 priority patent/US9752139B2/en

Links

Classifications

    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01F—MAGNETS; INDUCTANCES; TRANSFORMERS; SELECTION OF MATERIALS FOR THEIR MAGNETIC PROPERTIES
    • H01F7/00—Magnets
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/14—Scaffolds; Matrices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/16—Particles; Beads; Granular material; Encapsulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/06—Magnetic means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2529/00—Culture process characterised by the use of electromagnetic stimulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/10—Mineral substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

Abstract

The present invention is a culture apparatus and method for growing cells and tissue in a three-dimensional configuration using magnetic, paramagnetic, ferromagnetic and diamagnetic forces. Within this culture apparatus, the cells/tissue are grown with magnetized core particles and are suspended via magnetic forces in a native, non-restricted, three-dimensional configuration, while being maintained in a 1g growth environment without rotational alteration of the gravity vector.
PCT/US2004/020908 2003-06-30 2004-06-30 Diamagnetically stabilised three-dimensional cell culture WO2005003332A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US12/330,071 US20090137018A1 (en) 2003-06-30 2008-12-08 Magnetic three-dimensional cell culture apparatus and method
US14/096,791 US9752139B2 (en) 2003-06-30 2013-12-04 Magnetic three-dimensional cell culture apparatus and method

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US48104203P 2003-06-30 2003-06-30
US60/481,042 2003-06-30

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US30647805A Continuation 2003-06-30 2005-12-29

Publications (2)

Publication Number Publication Date
WO2005003332A2 WO2005003332A2 (en) 2005-01-13
WO2005003332A3 true WO2005003332A3 (en) 2005-09-09

Family

ID=33563823

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/020908 WO2005003332A2 (en) 2003-06-30 2004-06-30 Diamagnetically stabilised three-dimensional cell culture

Country Status (1)

Country Link
WO (1) WO2005003332A2 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090137018A1 (en) 2003-06-30 2009-05-28 University Of South Florida Magnetic three-dimensional cell culture apparatus and method
WO2005010139A2 (en) 2003-07-23 2005-02-03 University Of South Florida Ferromagnetic cell and tissue culture microcarriers
WO2010036957A1 (en) * 2008-09-25 2010-04-01 William Marsh Rice University Systems and methods for magnetic guidance and patterning of cells and materials
US10407660B2 (en) 2010-08-10 2019-09-10 Greiner Bio-One North America, Inc. Hardware for magnetic 3D culture
US20120020885A1 (en) * 2010-07-26 2012-01-26 Searete Llc, A Limited Liability Corporation Of The State Of Delaware MHC-Less cells
US10288603B2 (en) 2011-02-01 2019-05-14 Greiner Bio-One North America, Inc. 3D cell viability assay
BR112013025086A2 (en) * 2011-03-29 2016-08-23 Yongxin Zhang multifunctional bioreactor system and methods for cell classification and culture
CN105121621B (en) * 2012-10-18 2019-08-02 张永新 Bioreactor system and its method for cell sound state alternate culture
CN109153958A (en) * 2016-05-27 2019-01-04 株式会社日立高新技术 The producing device and production method of the stereoisomer of cell
JP7151218B2 (en) * 2018-07-04 2022-10-12 横河電機株式会社 Method for producing cell construct, carrier, and method for producing carrier
EP3911410A1 (en) 2019-01-18 2021-11-24 Voyager Therapeutics, Inc. Methods and systems for producing aav particles
EP4236974A2 (en) 2020-10-29 2023-09-06 RegenxBio Inc. Vectorized factor xii antibodies and administration thereof

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1982000660A1 (en) * 1980-08-20 1982-03-04 Mosbach K Immobilization of animal cells
US5026650A (en) * 1988-06-30 1991-06-25 The United States Of Amercia As Represented By The Administrator Of The National Aeronautics And Space Administration Horizontally rotated cell culture system with a coaxial tubular oxygenator
US6054319A (en) * 1998-02-03 2000-04-25 Board Of Trustees Operating Michigan State University Method and apparatus for growing cells using gas or liquid aphrons
WO2002051985A2 (en) * 2000-12-22 2002-07-04 Keele University Culturing tissue using magnetically generated mechanical stresses
WO2005010139A2 (en) * 2003-07-23 2005-02-03 University Of South Florida Ferromagnetic cell and tissue culture microcarriers

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1982000660A1 (en) * 1980-08-20 1982-03-04 Mosbach K Immobilization of animal cells
US5026650A (en) * 1988-06-30 1991-06-25 The United States Of Amercia As Represented By The Administrator Of The National Aeronautics And Space Administration Horizontally rotated cell culture system with a coaxial tubular oxygenator
US6054319A (en) * 1998-02-03 2000-04-25 Board Of Trustees Operating Michigan State University Method and apparatus for growing cells using gas or liquid aphrons
WO2002051985A2 (en) * 2000-12-22 2002-07-04 Keele University Culturing tissue using magnetically generated mechanical stresses
WO2005010139A2 (en) * 2003-07-23 2005-02-03 University Of South Florida Ferromagnetic cell and tissue culture microcarriers

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
"Diamagnetically stabilised levitation", 16 August 1999 (1999-08-16), XP002331831, Retrieved from the Internet <URL:http://www.hfml.ru.nl/fingertip.html> [retrieved on 20050606] *
GEIM A: "Everyone's magnetism", PHYSICS TODAY, September 1998 (1998-09-01), pages 36 - 39, XP008048406, ISSN: 0031-9228 *
SIMON M D ET AL.: "Diamagnetically stabilized levitation", AMERICAN JOURNAL OF PHYSICS, vol. 69, no. 6, June 2001 (2001-06-01), pages 702 - 713, XP002331832 *

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