WO2005021720A3 - Method of in vitro differentiation of neural stem cells, motor neurons and dopamine neurons from primate embryonic stem cells - Google Patents

Method of in vitro differentiation of neural stem cells, motor neurons and dopamine neurons from primate embryonic stem cells Download PDF

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Publication number
WO2005021720A3
WO2005021720A3 PCT/US2004/027841 US2004027841W WO2005021720A3 WO 2005021720 A3 WO2005021720 A3 WO 2005021720A3 US 2004027841 W US2004027841 W US 2004027841W WO 2005021720 A3 WO2005021720 A3 WO 2005021720A3
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Prior art keywords
stem cells
neurons
cells
vitro differentiation
embryonic stem
Prior art date
Application number
PCT/US2004/027841
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French (fr)
Other versions
WO2005021720A2 (en
Inventor
Su-Chun Zhang
James A Thomson
Ian David Duncan
Xue-Jun Li
Original Assignee
Wisconsin Alumni Res Found
Su-Chun Zhang
James A Thomson
Ian David Duncan
Xue-Jun Li
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wisconsin Alumni Res Found, Su-Chun Zhang, James A Thomson, Ian David Duncan, Xue-Jun Li filed Critical Wisconsin Alumni Res Found
Priority to AU2004269361A priority Critical patent/AU2004269361B2/en
Priority to JP2006524872A priority patent/JP2007503811A/en
Priority to EP04782339A priority patent/EP1670901A4/en
Priority to GB0605851A priority patent/GB2421029B/en
Priority to CA2536588A priority patent/CA2536588C/en
Publication of WO2005021720A2 publication Critical patent/WO2005021720A2/en
Publication of WO2005021720A3 publication Critical patent/WO2005021720A3/en
Priority to IL173832A priority patent/IL173832A/en
Priority to IL198450A priority patent/IL198450A/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
    • A61P25/16—Anti-Parkinson drugs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0619—Neurons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0623—Stem cells
    • C—CHEMISTRY; METALLURGY
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/44—Thiols, e.g. mercaptoethanol
    • C—CHEMISTRY; METALLURGY
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/46—Amines, e.g. putrescine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/119—Other fibroblast growth factors, e.g. FGF-4, FGF-8, FGF-10
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/13—Nerve growth factor [NGF]; Brain-derived neurotrophic factor [BDNF]; Cilliary neurotrophic factor [CNTF]; Glial-derived neurotrophic factor [GDNF]; Neurotrophins [NT]; Neuregulins
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/41—Hedgehog proteins; Cyclopamine (inhibitor)
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/90—Polysaccharides
    • C12N2501/91—Heparin
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Reproductive Health (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gynecology & Obstetrics (AREA)
  • Psychology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

A method of differentiating embryonic stem cells into neural and motor cells is disclosed. In one embodiment, the invention comprises culturing a population of cells comprising a majority of cells that are characterized by an early rosette morphology and are Sox1-/Pax6+ in the presence of FGF2, FGF4, FGF8, FGF9, or RA wherein the cells are characterized by an neural tube-like rosette morphology and are Pax6+/Sox1+.
PCT/US2004/027841 2003-08-29 2004-08-27 Method of in vitro differentiation of neural stem cells, motor neurons and dopamine neurons from primate embryonic stem cells WO2005021720A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
AU2004269361A AU2004269361B2 (en) 2003-08-29 2004-08-27 Method of in vitro differentiation of neural stem cells, motor neurons and dopamine neurons from primate embryonic stem cells
JP2006524872A JP2007503811A (en) 2003-08-29 2004-08-27 Method for in vitro differentiation of neural stem cells, motor neurons and dopamine neurons from primate embryonic stem cells
EP04782339A EP1670901A4 (en) 2003-08-29 2004-08-27 Method of in vitro differentiation of neural stem cells, motor neurons and dopamine neurons from primate embryonic stem cells
GB0605851A GB2421029B (en) 2003-08-29 2004-08-27 Method of in vitro differentiation of neural stem cells, motor neurons and dopamine neurons from primate embryonic stem cells
CA2536588A CA2536588C (en) 2003-08-29 2004-08-27 Method of in vitro differentiation of neural stem cells, motor neurons and dopamine neurons from primate embryonic stem cells
IL173832A IL173832A (en) 2003-08-29 2006-02-20 Method of in vitro differentiation of neural stem cells, motor neurons and dopamine neurons from primate embryonic stem cells
IL198450A IL198450A (en) 2003-08-29 2009-04-28 Populations of synchronized neural stem cells and methods of producing the same

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US49883103P 2003-08-29 2003-08-29
US60/498,831 2003-08-29
US49957003P 2003-09-02 2003-09-02
US60/499,570 2003-09-02

Publications (2)

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WO2005021720A2 WO2005021720A2 (en) 2005-03-10
WO2005021720A3 true WO2005021720A3 (en) 2005-05-19

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PCT/US2004/027841 WO2005021720A2 (en) 2003-08-29 2004-08-27 Method of in vitro differentiation of neural stem cells, motor neurons and dopamine neurons from primate embryonic stem cells

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EP (1) EP1670901A4 (en)
JP (2) JP2007503811A (en)
KR (2) KR20080109943A (en)
AU (1) AU2004269361B2 (en)
CA (1) CA2536588C (en)
GB (1) GB2421029B (en)
IL (2) IL173832A (en)
WO (1) WO2005021720A2 (en)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7588937B2 (en) 2001-10-03 2009-09-15 Wisconsin Alumni Research Foundation Method of in vitro differentiation of neural stem cells, motor neurons and dopamine neurons from primate embryonic stem cells
US8153424B2 (en) 2001-10-03 2012-04-10 Wisconsin Alumni Research Foundation Method of in vitro differentiation of neural stem cells, motor neurons and dopamine neurons from primate embryonic stem cells
US7632679B2 (en) 2002-07-16 2009-12-15 The Trustees Of Columbia University In The City Of New York Systems and methods for screening for modulators of neural differentiation
JP4792290B2 (en) * 2005-12-27 2011-10-12 財団法人 東京都医学総合研究所 Method for producing neural stem cells
EP2422794A3 (en) 2006-03-07 2013-03-13 Geeta Shroff Compositions comprising human embryonic stem cells and their derivatives, methods of use, and methods of preparation
KR100838013B1 (en) * 2006-06-07 2008-06-12 제일약품주식회사 Efficient Generation of Neural Progenitors, Neurons and Dopaminergic Neurons from Human Embryonic Stem Cells
WO2009132156A1 (en) 2008-04-22 2009-10-29 Regenerative Research Foundation Retinal pigment epithelial stem cells
KR101168053B1 (en) * 2009-11-06 2012-07-24 연세대학교 산학협력단 Efficient and Universal Method for Neural Differentiation of Pluripotent Stem Cells
US20130149287A1 (en) 2010-07-30 2013-06-13 Cambridge Enterprise Limited Corticogenesis of Human Pluripotent Cells
WO2012135621A2 (en) * 2011-03-30 2012-10-04 Cellular Dynamics International. Inc Priming of pluripotent stem cells for neural differentiation
JP6015112B2 (en) * 2012-05-11 2016-10-26 株式会社ニコン Cell evaluation apparatus, cell evaluation method and program
ES2852998T3 (en) * 2015-11-12 2021-09-14 Hoffmann La Roche Oligonucleotides to induce expression of paternal UBE3A
CA3013357A1 (en) 2016-02-01 2017-08-10 Cedars-Sinai Medical Center Systems and methods for growth of intestinal cells in microfluidic devices
JPWO2017209290A1 (en) * 2016-06-03 2019-04-11 国立大学法人京都大学 Production and use of pluripotent stem cell-derived motor neurons
JP6376196B2 (en) * 2016-09-29 2018-08-22 株式会社ニコン Cell evaluation apparatus, cell evaluation method and program
WO2018140647A1 (en) 2017-01-25 2018-08-02 Cedars-Sinai Medical Center In vitro induction of mammary-like differentiation from human pluripotent stem cells
US11767513B2 (en) 2017-03-14 2023-09-26 Cedars-Sinai Medical Center Neuromuscular junction
WO2018176001A2 (en) 2017-03-24 2018-09-27 Cedars-Sinai Medical Center Methods and compositions for production of fallopian tube epithelium
CN108048399B (en) * 2017-11-13 2021-03-23 昆明医科大学 Method for inducing tree shrew dopamine neuron primary cell autophagy by METH
JP2019106895A (en) * 2017-12-15 2019-07-04 インダストリー‐ユニバーシティー コーぺレーション ファンデーション ハンヤン ユニバーシティ Method of differentiating neural stem cells or neural precursor cells into dopamine neurons
US20210130774A1 (en) * 2018-04-06 2021-05-06 Cedars-Sinai Medical Center Human pluripotent stem cell derived neurodegenerative disease models on a microfluidic chip
KR20240033089A (en) * 2018-06-06 2024-03-12 더 리젠츠 오브 더 유니버시티 오브 캘리포니아 Neural stem cell compositions and methods to treat neurodegenerative disorders
JP6798533B2 (en) * 2018-07-26 2020-12-09 株式会社ニコン Cell evaluation device, incubator and program
CA3152504A1 (en) * 2019-09-06 2021-03-11 Keio University Method for producing cell aggregate including glial progenitor cells
JP7235027B2 (en) * 2020-11-16 2023-03-08 株式会社ニコン Cell evaluation instruments, incubators and programs

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020009743A1 (en) * 2000-05-17 2002-01-24 Carpenter Melissa K. Neural progenitor cell populations
US20020168763A1 (en) * 2000-11-30 2002-11-14 Yan Wen Liang Isolated homozygous stem cells, differentiated cells derived therefrom, and materials and methods for making and using same
US20030211605A1 (en) * 2001-05-01 2003-11-13 Lee Sang-Hun Derivation of midbrain dopaminergic neurons from embryonic stem cells

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7144997B2 (en) * 1997-07-24 2006-12-05 Curis, Inc. Vertebrate embryonic patterning-inducing proteins, compositions and uses related therto
AU743251B2 (en) * 1997-08-04 2002-01-24 Regents Of The University Of California, The Methods for treating neurological deficits
WO2001083715A2 (en) * 2000-05-01 2001-11-08 THE GOVERNMENT OF THE UNITED STATES OF AMERICA as represented by the Secretary, Derivation of midbrain dopaminergic neurons from embryonic stem cells
US6887706B2 (en) * 2001-10-03 2005-05-03 Wisconsin Alumni Research Foundation Method of in vitro differentiation of transplantable neural precursor cells from primate embryonic stem cells

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020009743A1 (en) * 2000-05-17 2002-01-24 Carpenter Melissa K. Neural progenitor cell populations
US20020168763A1 (en) * 2000-11-30 2002-11-14 Yan Wen Liang Isolated homozygous stem cells, differentiated cells derived therefrom, and materials and methods for making and using same
US20030211605A1 (en) * 2001-05-01 2003-11-13 Lee Sang-Hun Derivation of midbrain dopaminergic neurons from embryonic stem cells

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ZHANG S.-C. ET AL.: "In vitro differentiation of transplantable neural precursors for human embryonic stem cells", NAT. BIOTECHNOL., vol. 19, no. 12, December 2001 (2001-12-01), pages 1129 - 1133, XP002971361 *

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GB0605851D0 (en) 2006-05-03
CA2536588A1 (en) 2005-03-10
GB2421029A (en) 2006-06-14
EP1670901A2 (en) 2006-06-21
KR20080109943A (en) 2008-12-17
KR20060115351A (en) 2006-11-08
JP2010162024A (en) 2010-07-29
CA2536588C (en) 2018-04-24
IL173832A (en) 2011-02-28
JP5529561B2 (en) 2014-06-25
WO2005021720A2 (en) 2005-03-10
GB2421029B (en) 2008-04-09
EP1670901A4 (en) 2007-09-05
IL198450A (en) 2011-01-31
AU2004269361B2 (en) 2010-05-20
IL173832A0 (en) 2006-07-05
AU2004269361A1 (en) 2005-03-10
JP2007503811A (en) 2007-03-01

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