WO2005022113A3 - Ion channel assay methods - Google Patents
Ion channel assay methods Download PDFInfo
- Publication number
- WO2005022113A3 WO2005022113A3 PCT/US2004/022047 US2004022047W WO2005022113A3 WO 2005022113 A3 WO2005022113 A3 WO 2005022113A3 US 2004022047 W US2004022047 W US 2004022047W WO 2005022113 A3 WO2005022113 A3 WO 2005022113A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- ion channel
- assay methods
- channel assay
- candidate compound
- cells
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 2
- 238000002780 ion channel assay Methods 0.000 title 1
- 150000001875 compounds Chemical class 0.000 abstract 2
- 102000004310 Ion Channels Human genes 0.000 abstract 1
- 230000004071 biological effect Effects 0.000 abstract 1
- 230000001419 dependent effect Effects 0.000 abstract 1
- 230000005684 electric field Effects 0.000 abstract 1
- 230000003252 repetitive effect Effects 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
- G01N33/5038—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects involving detection of metabolites per se
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
- G01N33/48728—Investigating individual cells, e.g. by patch clamp, voltage clamp
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5076—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving cell organelles, e.g. Golgi complex, endoplasmic reticulum
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5082—Supracellular entities, e.g. tissue, organisms
- G01N33/5088—Supracellular entities, e.g. tissue, organisms of vertebrates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6872—Intracellular protein regulatory factors and their receptors, e.g. including ion channels
Abstract
Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2006520237A JP4768611B2 (en) | 2003-07-14 | 2004-07-08 | Ion channel assay method |
EP04801966A EP1644497A4 (en) | 2003-07-14 | 2004-07-08 | Ion channel assay methods |
CA2532973A CA2532973C (en) | 2003-07-14 | 2004-07-08 | Ion channel assay methods |
AU2004269322A AU2004269322B2 (en) | 2003-07-14 | 2004-07-08 | Ion channel assay methods |
AU2010246565A AU2010246565B2 (en) | 2003-07-14 | 2010-12-01 | Ion channel assay methods |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US10/620,312 | 2003-07-14 | ||
US10/620,312 US7615356B2 (en) | 2000-07-10 | 2003-07-14 | Ion channel assay methods |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2005022113A2 WO2005022113A2 (en) | 2005-03-10 |
WO2005022113A3 true WO2005022113A3 (en) | 2005-05-19 |
Family
ID=34273220
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2004/022047 WO2005022113A2 (en) | 2003-07-14 | 2004-07-08 | Ion channel assay methods |
Country Status (6)
Country | Link |
---|---|
US (4) | US7615356B2 (en) |
EP (1) | EP1644497A4 (en) |
JP (2) | JP4768611B2 (en) |
AU (2) | AU2004269322B2 (en) |
CA (1) | CA2532973C (en) |
WO (1) | WO2005022113A2 (en) |
Families Citing this family (35)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7615356B2 (en) * | 2000-07-10 | 2009-11-10 | Vertex Pharmaceuticals (San Diego) Llc | Ion channel assay methods |
US7399599B2 (en) * | 2000-07-10 | 2008-07-15 | Vertex Pharmaceuticals (San Diego) Llc | Ion channel assay methods |
AUPS192302A0 (en) * | 2002-04-23 | 2002-05-30 | Cochlear Limited | Electrode array for a cochlear implant having one or more adjustable electrodes |
EP1869451B1 (en) | 2005-03-08 | 2013-06-26 | Life Technologies Corporation | Monitoring and manipulating cellular transmembrane potentials using nanostructures |
WO2007041684A2 (en) * | 2005-10-04 | 2007-04-12 | The Forsyth Institute | Ion flux in biological processes, and methods related thereto |
US20090032411A1 (en) * | 2006-02-10 | 2009-02-05 | Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. | Method and system for detecting pharmacologically active substances by measuring membrane currents with extracellular sensors |
WO2008013969A2 (en) * | 2006-07-28 | 2008-01-31 | Senomyx, Inc. | IMPROVED CELL-BASED FLUORESCENT ASSAYS FOR IDENTIFYING ALPHA AND DELTA ENaC MODULATORS |
ES2355528T3 (en) * | 2006-09-18 | 2011-03-28 | The University Of North Texas | TEST PLATFORM OF MULTIPLE NETWORK NERVOUS CELLS WITH PARALLEL REGISTRATION CAPACITY. |
US7994174B2 (en) | 2007-09-19 | 2011-08-09 | Vertex Pharmaceuticals Incorporated | Pyridyl sulfonamides as modulators of ion channels |
JP4825768B2 (en) * | 2007-10-03 | 2011-11-30 | 三菱重工鉄構エンジニアリング株式会社 | Coral photosynthetic activity evaluation system |
WO2010002540A2 (en) * | 2008-06-05 | 2010-01-07 | Life Technologies Corporation | Activation and monitoring of cellular transmembrane potentials |
US20120053084A1 (en) * | 2008-09-04 | 2012-03-01 | Galenea Corp. | Synaptic vesicle cycling assays and systems |
US8623496B2 (en) * | 2009-11-06 | 2014-01-07 | Wisconsin Alumni Research Foundation | Laser drilling technique for creating nanoscale holes |
US8957663B2 (en) | 2009-12-01 | 2015-02-17 | Nipro Corporation | Cellular potential measurement container and production method therefor |
US20110288545A1 (en) * | 2010-04-22 | 2011-11-24 | Old Dominion University Research Foundation | Method and Device for Ablation of Cancer and Resistance to New Cancer Growth |
CN103228793B (en) * | 2010-06-29 | 2017-02-15 | 公立大学法人名古屋市立大学 | Material for screening for compound capable of acting on ion channel, and use thereof |
JP5778354B2 (en) | 2012-10-25 | 2015-09-16 | 浜松ホトニクス株式会社 | Cell observation device, electrical stimulation device, and cell observation method |
EP2913391B1 (en) | 2012-10-25 | 2020-07-01 | Hamamatsu Photonics K.K. | Cell observation device, and cell observation method |
WO2014093980A1 (en) | 2012-12-14 | 2014-06-19 | Vala Sciences, Inc. | Analysis of action potentials, transients, and ion flux in excitable cells |
CN104884632B (en) | 2012-12-27 | 2018-01-02 | 索尼公司 | cell analysis system and cell analysis method |
EP3290082A3 (en) | 2013-06-03 | 2018-06-06 | Pulse Biosciences, Inc. | Methods and devices for stimulating an immune response using nanosecond pulsed electric fields |
US9279801B2 (en) * | 2013-07-26 | 2016-03-08 | Axion Biosystems, Inc. | Devices, systems and methods for high-throughput electrophysiology |
CN105683355B (en) * | 2013-11-08 | 2018-07-06 | 索尼公司 | Cell analysis system, cell analysis program and cell analysis method |
US10415010B2 (en) | 2014-01-16 | 2019-09-17 | Hamamatsu Photonics K.K. | Cell observation device, electrostimulation device, and cell observation method |
JP6159486B2 (en) * | 2014-06-12 | 2017-07-05 | アクシオン バイオシステムズ, インコーポレイテッド | Multiwell microelectrode array with optical stimulation |
US10067117B2 (en) | 2014-08-12 | 2018-09-04 | Axion Biosystems, Inc. | Cell-based biosensor array and associated methods for manufacturing the same |
WO2016089781A1 (en) | 2014-12-01 | 2016-06-09 | Electroblate, Inc. | Nanoelectroablation control and vaccination |
US11515007B2 (en) * | 2016-03-11 | 2022-11-29 | Merck Sharp & Dohme Llc | In silico process for selecting protein formulation excipients |
WO2019014603A1 (en) * | 2017-07-14 | 2019-01-17 | StemoniX Inc. | High throughput optical assay of human mixed cell population spheroids |
CN109187504A (en) * | 2018-07-11 | 2019-01-11 | 佛山市顺德区欧罗拉生物科技有限公司 | A kind of ion transport pathway activity assays based on ion channel reader |
ES2887832B2 (en) * | 2020-06-23 | 2022-12-28 | Consejo Superior Investigacion | NANOSTRUCTURED ELECTRODES FOR THE ELECTRICAL STIMULATION OF CELLS IN CULTURE, DEVICES, SYSTEMS AND ASSOCIATED PROCEDURES |
JP2024501847A (en) * | 2020-12-31 | 2024-01-16 | キュー-ステート バイオサイエンシーズ, インコーポレイテッド | plate imager |
CN113607707B (en) * | 2021-08-05 | 2024-03-12 | 国家食品安全风险评估中心 | Ocean neurotoxin fluorescence rapid screening method based on sodium ion channel Nav1.1 |
WO2023150302A1 (en) * | 2022-02-03 | 2023-08-10 | Correlia Biosystems, Inc. | System modules for use with fluid sample processing systems |
CN115015138A (en) * | 2022-05-18 | 2022-09-06 | 药明激创(佛山)生物科技有限公司 | Ion channel-based drug screening device and method |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1996041166A2 (en) * | 1995-06-07 | 1996-12-19 | The Regents Of The University Of California | Detection of transmembrane potentials by optical methods |
Family Cites Families (55)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4067951A (en) | 1975-11-19 | 1978-01-10 | Bactomatic Inc. | Process for making impedance measuring module |
US4461304A (en) | 1979-11-05 | 1984-07-24 | Massachusetts Institute Of Technology | Microelectrode and assembly for parallel recording of neurol groups |
CS224317B1 (en) * | 1981-03-13 | 1984-01-16 | Cyril Doc Dr Csc Simecek | Pulmonary ventilation evaluating apparatus |
US4514500A (en) | 1982-11-19 | 1985-04-30 | General Electric Company | Cell growth on liquid-liquid interfaces |
DE3325843A1 (en) | 1983-07-18 | 1985-02-07 | Kernforschungsanlage Jülich GmbH, 5170 Jülich | METHOD AND DEVICE FOR DIFFERENTIATING PARTICLES OR PARTICLES IN A MEDIUM |
DE3522610A1 (en) | 1985-06-12 | 1986-12-18 | Kernforschungsanlage Jülich GmbH, 5170 Jülich | CHAMBER FOR THE TREATMENT OF CELLS IN THE ELECTRIC FIELD |
US4628933A (en) | 1985-07-23 | 1986-12-16 | Michelson Robin P | Method and apparatus for visual prosthesis |
US4677989A (en) | 1986-02-06 | 1987-07-07 | Eic Laboratories, Inc. | Iridium oxide coated electrodes for neural stimulation |
US4695547A (en) | 1986-04-02 | 1987-09-22 | Jeffrey L. Hilliard | Probe for electrofusion, electroporation, or like procedure |
US4969468A (en) | 1986-06-17 | 1990-11-13 | Alfred E. Mann Foundation For Scientific Research | Electrode array for use in connection with a living body and method of manufacture |
US5128257A (en) | 1987-08-31 | 1992-07-07 | Baer Bradford W | Electroporation apparatus and process |
US4970154A (en) | 1987-10-09 | 1990-11-13 | Baylor College Of Medicine | Method for inserting foreign genes into cells using pulsed radiofrequency |
US5178161A (en) | 1988-09-02 | 1993-01-12 | The Board Of Trustees Of The Leland Stanford Junior University | Microelectronic interface |
US5024223A (en) | 1989-08-08 | 1991-06-18 | Chow Alan Y | Artificial retina device |
US5643742A (en) | 1990-04-03 | 1997-07-01 | Cellstat Technologies, Inc. | System for electronically monitoring and recording cell cultures |
GB2244135B (en) | 1990-05-04 | 1994-07-13 | Gen Electric Co Plc | Sensor devices |
DE4207368A1 (en) | 1991-08-06 | 1993-02-11 | Biotronik Mess & Therapieg | VOICE ELECTRODE |
US5187096A (en) | 1991-08-08 | 1993-02-16 | Rensselaer Polytechnic Institute | Cell substrate electrical impedance sensor with multiple electrode array |
US5193540A (en) | 1991-12-18 | 1993-03-16 | Alfred E. Mann Foundation For Scientific Research | Structure and method of manufacture of an implantable microstimulator |
US5545130A (en) | 1992-04-08 | 1996-08-13 | Genetronics, Inc. | Flow through electroporation method |
JP3099049B2 (en) | 1992-07-29 | 2000-10-16 | 農林水産省果樹試験場長 | High-throughput electrodes for electrical cell fusion and electrical nucleic acid transfer |
US5432086A (en) | 1992-11-18 | 1995-07-11 | Sy-Lab Vertriebsgellschaft M.B.H. | Apparatus for the automatic monitoring of microorganism culture |
US5439440A (en) | 1993-04-01 | 1995-08-08 | Genetronics, Inc. | Electroporation system with voltage control feedback for clinical applications |
US5810725A (en) | 1993-04-16 | 1998-09-22 | Matsushita Electric Industrial Co., Ltd. | Planar electrode |
US5422272A (en) | 1993-07-14 | 1995-06-06 | Andrew A. Papp | Improvements to apparatus and method for electroporation |
US5437982A (en) | 1993-09-08 | 1995-08-01 | University Of Washington | Methods of identifying specific inactivation gate inhibitors of the sodium channel |
US5965452A (en) | 1996-07-09 | 1999-10-12 | Nanogen, Inc. | Multiplexed active biologic array |
US5591139A (en) | 1994-06-06 | 1997-01-07 | The Regents Of The University Of California | IC-processed microneedles |
US5563067A (en) | 1994-06-13 | 1996-10-08 | Matsushita Electric Industrial Co., Ltd. | Cell potential measurement apparatus having a plurality of microelectrodes |
AU2788695A (en) | 1994-10-28 | 1996-05-23 | Neurosearch A/S | Patch clamp apparatus and technique having high throughput and low fluid volume requirements |
US5545120A (en) * | 1995-01-18 | 1996-08-13 | Medical Media Systems | Endoscopic viewing system for maintaining a surgeon's normal sense of kinesthesia during endoscopic surgery regardless of the orientation of the endoscope vis-a-vis the surgeon |
DE19529371C3 (en) | 1995-08-10 | 2003-05-28 | Nmi Univ Tuebingen | Microelectrode array |
AU721291C (en) | 1996-05-15 | 2003-02-27 | Hyperion Catalysis International, Inc. | Graphitic nanofibers in electrochemical capacitors |
BR9710708A (en) | 1996-05-15 | 1999-08-17 | Hyperion Catalysis Int | Nanofibers with high surface area |
DE19646505A1 (en) | 1996-11-12 | 1998-05-14 | Itt Ind Gmbh Deutsche | Device for carrying out tests on cell samples and the like |
US5957958A (en) | 1997-01-15 | 1999-09-28 | Advanced Bionics Corporation | Implantable electrode arrays |
US5972694A (en) | 1997-02-11 | 1999-10-26 | Mathus; Gregory | Multi-well plate |
US5935156A (en) * | 1997-03-12 | 1999-08-10 | Microleve International, Ltd. | Method and apparatus for administering microcurrent electrotherapy treatment |
EP0866123B1 (en) | 1997-03-21 | 2005-03-16 | Eppendorf Ag | Method and circuit device for electropermeation or electroporation of living cells |
US5981268A (en) | 1997-05-30 | 1999-11-09 | Board Of Trustees, Leland Stanford, Jr. University | Hybrid biosensors |
US6205016B1 (en) | 1997-06-04 | 2001-03-20 | Hyperion Catalysis International, Inc. | Fibril composite electrode for electrochemical capacitors |
US6024702A (en) | 1997-09-03 | 2000-02-15 | Pmt Corporation | Implantable electrode manufactured with flexible printed circuit |
US6038478A (en) | 1997-10-16 | 2000-03-14 | Huntingdon Medical Research Institutes | Lymphocyte attraction by electrical stimulation |
US6046002A (en) | 1998-01-05 | 2000-04-04 | The Board Of Trustees Of The Leland Stanford Junior University | Highly parallel and sensitive method for identifying drugs and drug targets |
JP3391269B2 (en) * | 1998-01-20 | 2003-03-31 | 株式会社村田製作所 | Dielectric ceramic and its manufacturing method, and multilayer ceramic electronic component and its manufacturing method |
US6009347A (en) | 1998-01-27 | 1999-12-28 | Genetronics, Inc. | Electroporation apparatus with connective electrode template |
JP4164233B2 (en) | 1998-03-12 | 2008-10-15 | 株式会社東京大学Tlo | Automatic micromembrane potential measuring device |
US5935155A (en) | 1998-03-13 | 1999-08-10 | John Hopkins University, School Of Medicine | Visual prosthesis and method of using same |
EP1125120A1 (en) | 1998-10-27 | 2001-08-22 | President And Fellows of Harvard College | Biological ion channels in nanofabricated detectors |
WO2000034434A1 (en) | 1998-12-07 | 2000-06-15 | Acacia Biosciences, Inc. | Multi-channel electrode arrays |
US20030170606A1 (en) | 1999-05-10 | 2003-09-11 | Shu-Gui Huang | High-throughput screening assays for modulators of mitochondrial membrane potential |
US7399599B2 (en) | 2000-07-10 | 2008-07-15 | Vertex Pharmaceuticals (San Diego) Llc | Ion channel assay methods |
AU2001275881B2 (en) * | 2000-07-10 | 2006-04-06 | Vertex Pharmaceuticals (San Diego) Llc | Ion channel assay methods |
US7615356B2 (en) | 2000-07-10 | 2009-11-10 | Vertex Pharmaceuticals (San Diego) Llc | Ion channel assay methods |
CA2453489A1 (en) * | 2001-07-12 | 2003-01-23 | Merck & Co., Inc. | Electrical field stimulation of eukaryotic cells |
-
2003
- 2003-07-14 US US10/620,312 patent/US7615356B2/en not_active Expired - Lifetime
-
2004
- 2004-07-08 CA CA2532973A patent/CA2532973C/en active Active
- 2004-07-08 WO PCT/US2004/022047 patent/WO2005022113A2/en active Search and Examination
- 2004-07-08 AU AU2004269322A patent/AU2004269322B2/en not_active Ceased
- 2004-07-08 JP JP2006520237A patent/JP4768611B2/en active Active
- 2004-07-08 EP EP04801966A patent/EP1644497A4/en not_active Ceased
-
2006
- 2006-05-31 US US11/444,214 patent/US7611850B2/en not_active Expired - Lifetime
- 2006-05-31 US US11/443,697 patent/US7923537B2/en not_active Expired - Lifetime
-
2009
- 2009-09-28 US US12/568,604 patent/US8426201B2/en not_active Expired - Fee Related
-
2010
- 2010-12-01 AU AU2010246565A patent/AU2010246565B2/en not_active Ceased
-
2011
- 2011-04-13 JP JP2011089637A patent/JP5588914B2/en active Active
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1996041166A2 (en) * | 1995-06-07 | 1996-12-19 | The Regents Of The University Of California | Detection of transmembrane potentials by optical methods |
Non-Patent Citations (1)
Title |
---|
SINHA S.R. ET AL.: "Simultaneous optical recording of evoked and spontaneous transients of membrane potential and intracellular calcium concentration with high spatio-temporal resolution", J. NEUROSCI. METHODS, vol. 60, no. 1-2, August 1995 (1995-08-01), pages 49 - 60, XP002986061 * |
Also Published As
Publication number | Publication date |
---|---|
AU2010246565A1 (en) | 2010-12-23 |
AU2004269322A1 (en) | 2005-03-10 |
US7611850B2 (en) | 2009-11-03 |
EP1644497A4 (en) | 2007-08-08 |
US20060281069A1 (en) | 2006-12-14 |
US20060216688A1 (en) | 2006-09-28 |
US20040110123A1 (en) | 2004-06-10 |
US20100081163A1 (en) | 2010-04-01 |
AU2010246565B2 (en) | 2011-11-24 |
JP4768611B2 (en) | 2011-09-07 |
US7615356B2 (en) | 2009-11-10 |
US8426201B2 (en) | 2013-04-23 |
WO2005022113A2 (en) | 2005-03-10 |
JP5588914B2 (en) | 2014-09-10 |
EP1644497A2 (en) | 2006-04-12 |
CA2532973A1 (en) | 2005-03-10 |
JP2007534927A (en) | 2007-11-29 |
US7923537B2 (en) | 2011-04-12 |
JP2011174939A (en) | 2011-09-08 |
CA2532973C (en) | 2013-09-10 |
AU2004269322B2 (en) | 2010-09-02 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
WO2005022113A3 (en) | Ion channel assay methods | |
WO2002008748A3 (en) | Ion channel assay methods | |
EP1820021B8 (en) | Improved method of electrochemical analysis of an analyte | |
WO2002077264A3 (en) | Arrayed transfection method and uses related thereto | |
WO2004092708A3 (en) | Methods for the electrochemical detection of target compounds | |
AU2003287527A1 (en) | Single cell analysis of membrane molecules | |
DE60238493D1 (en) | IS OF RARE CELLS USED MARKED CELLS | |
AU6345700A (en) | Derivative nucleic acids and uses thereof | |
DE60031997D1 (en) | UMKEHRTRANSFEKTIONSVERFAHREN | |
AU2003302134A1 (en) | Methods, nucleic acid constructs and cells for treating neurodegenerative disorders | |
WO2009013219A3 (en) | Coil structure for electromagnetic stimulation of a process within a living organism, device using such coil structure and method of driving | |
ATE267396T1 (en) | IDENTIFICATION OF CELLS DURING KINETIC EXPERIMENTS | |
WO2004108208A3 (en) | Methods and apparatus for conversion of eukaryotic cells by application of electric and magnetic fields | |
NO20024048D0 (en) | Method of operation of an electrolytic cell and means for the same | |
WO2003076897A3 (en) | Multiplexed analysis of cellular responses using endogenous reporter genes | |
AU2002244757A1 (en) | Method for specifically detecting, isolating and characterizing cells from body samples by transfecting nucleic acid constructs | |
AU2003303869A1 (en) | Methods for nucleic acid analysis | |
AUPS064202A0 (en) | Improved method for the analysis of nucleic acid samples | |
AU2003205817A1 (en) | Methods of nucleic acid amplification | |
AU2003234210A1 (en) | Direct cell target analysis | |
WO2005001416A3 (en) | High throughput assay of lp-pla2 activity | |
WO2004044175A3 (en) | Fluorescence guided cell capture | |
WO2004079332A3 (en) | Composition and method for increasing efficiency of introduction of target substance into cell | |
AU2003214440A1 (en) | Method of electrochemical cell analysis | |
WO2006081580A3 (en) | System for and method of patch clamp analysis |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AK | Designated states |
Kind code of ref document: A2 Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NA NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW |
|
AL | Designated countries for regional patents |
Kind code of ref document: A2 Designated state(s): BW GH GM KE LS MW MZ NA SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG |
|
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
WWE | Wipo information: entry into national phase |
Ref document number: 2532973 Country of ref document: CA Ref document number: 2004269322 Country of ref document: AU Ref document number: 2006520237 Country of ref document: JP |
|
REEP | Request for entry into the european phase |
Ref document number: 2004801966 Country of ref document: EP |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2004801966 Country of ref document: EP |
|
ENP | Entry into the national phase |
Ref document number: 2004269322 Country of ref document: AU Date of ref document: 20040708 Kind code of ref document: A |
|
WWP | Wipo information: published in national office |
Ref document number: 2004269322 Country of ref document: AU |
|
WWP | Wipo information: published in national office |
Ref document number: 2004801966 Country of ref document: EP |
|
DPEN | Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed from 20040101) |