WO2005029039A3 - Non-radioactive inositol phosphate assay for phospholipase c-coupled drug target screening - Google Patents

Non-radioactive inositol phosphate assay for phospholipase c-coupled drug target screening Download PDF

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Publication number
WO2005029039A3
WO2005029039A3 PCT/US2004/030749 US2004030749W WO2005029039A3 WO 2005029039 A3 WO2005029039 A3 WO 2005029039A3 US 2004030749 W US2004030749 W US 2004030749W WO 2005029039 A3 WO2005029039 A3 WO 2005029039A3
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WO
WIPO (PCT)
Prior art keywords
phospholipase
drug target
inositol phosphate
target screening
phosphate assay
Prior art date
Application number
PCT/US2004/030749
Other languages
French (fr)
Other versions
WO2005029039A2 (en
Inventor
Paul O Neilsen
Edson Assis
Angie M Branch
Beth E Drees
Original Assignee
Echelon Biosciences Inc
Paul O Neilsen
Edson Assis
Angie M Branch
Beth E Drees
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Echelon Biosciences Inc, Paul O Neilsen, Edson Assis, Angie M Branch, Beth E Drees filed Critical Echelon Biosciences Inc
Publication of WO2005029039A2 publication Critical patent/WO2005029039A2/en
Publication of WO2005029039A3 publication Critical patent/WO2005029039A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/72—Assays involving receptors, cell surface antigens or cell surface determinants for hormones
    • G01N2333/726—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH

Abstract

A non-radioactive assay which is amenable to high throughput (HT) screening for antagonists of PLC- activated Gαq receptors and/or tyrosine kinase coupled receptors comprising the steps of measuring longer-lived Ins(1,4,5)P3 metabolites (IPn s), i.e., Ins(1,3,4,5)P4 and Ins(1,4)P2, as a means to report the PLC activity in cells or cell lysates.
PCT/US2004/030749 2003-09-19 2004-09-20 Non-radioactive inositol phosphate assay for phospholipase c-coupled drug target screening WO2005029039A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US50444603P 2003-09-19 2003-09-19
US60/504,446 2003-09-19

Publications (2)

Publication Number Publication Date
WO2005029039A2 WO2005029039A2 (en) 2005-03-31
WO2005029039A3 true WO2005029039A3 (en) 2005-06-02

Family

ID=34375497

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/030749 WO2005029039A2 (en) 2003-09-19 2004-09-20 Non-radioactive inositol phosphate assay for phospholipase c-coupled drug target screening

Country Status (1)

Country Link
WO (1) WO2005029039A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9697994B1 (en) * 2015-04-09 2017-07-04 Ricarez Biosciences, LLC Use of stable label compounds with accelerator mass spectrometry

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000075662A1 (en) * 1999-06-09 2000-12-14 Ljl Biosystems, Inc. Cell-signaling assays
US20040106158A1 (en) * 2002-10-21 2004-06-03 Tabassum Naqvi IP3 protein binding assay

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000075662A1 (en) * 1999-06-09 2000-12-14 Ljl Biosystems, Inc. Cell-signaling assays
US20040106158A1 (en) * 2002-10-21 2004-06-03 Tabassum Naqvi IP3 protein binding assay

Also Published As

Publication number Publication date
WO2005029039A2 (en) 2005-03-31

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