WO2005047521A3 - Methods of preparing nucleic acid for detection - Google Patents

Methods of preparing nucleic acid for detection Download PDF

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Publication number
WO2005047521A3
WO2005047521A3 PCT/US2004/037488 US2004037488W WO2005047521A3 WO 2005047521 A3 WO2005047521 A3 WO 2005047521A3 US 2004037488 W US2004037488 W US 2004037488W WO 2005047521 A3 WO2005047521 A3 WO 2005047521A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
detection
methods
preparing nucleic
sample
Prior art date
Application number
PCT/US2004/037488
Other languages
French (fr)
Other versions
WO2005047521A2 (en
Inventor
Yeon K Choi
Michael S Zwick
Kent Mccue
Original Assignee
Investigen Inc
Yeon K Choi
Michael S Zwick
Kent Mccue
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Investigen Inc, Yeon K Choi, Michael S Zwick, Kent Mccue filed Critical Investigen Inc
Priority to JP2006539800A priority Critical patent/JP2007512811A/en
Priority to EP04818684A priority patent/EP1694869A2/en
Publication of WO2005047521A2 publication Critical patent/WO2005047521A2/en
Publication of WO2005047521A3 publication Critical patent/WO2005047521A3/en
Priority to US11/429,746 priority patent/US20070042400A1/en
Priority to US12/082,573 priority patent/US20080286786A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA

Abstract

Methods of preparing nucleic acid from polysaccharide-containing samples for detection by providing one or more glycosidases to the sample to degrade polysaccharides are provided. The nucleic acids can further be extracted from the sample. The method is particularly useful for detecting nucleic acid in samples with high starch content.
PCT/US2004/037488 2003-11-10 2004-11-10 Methods of preparing nucleic acid for detection WO2005047521A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2006539800A JP2007512811A (en) 2003-11-10 2004-11-10 Methods for preparing nucleic acids for detection
EP04818684A EP1694869A2 (en) 2003-11-10 2004-11-10 Methods of preparing nucleic acid for detection
US11/429,746 US20070042400A1 (en) 2003-11-10 2006-05-08 Methods of preparing nucleic acid for detection
US12/082,573 US20080286786A1 (en) 2003-11-10 2008-04-11 Methods of preparing nucleic acid for detection

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US51889503P 2003-11-10 2003-11-10
US60/518,895 2003-11-10
US55658404P 2004-03-25 2004-03-25
US60/556,584 2004-03-25

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US11/429,746 Continuation US20070042400A1 (en) 2003-11-10 2006-05-08 Methods of preparing nucleic acid for detection

Publications (2)

Publication Number Publication Date
WO2005047521A2 WO2005047521A2 (en) 2005-05-26
WO2005047521A3 true WO2005047521A3 (en) 2005-10-06

Family

ID=34594937

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/037488 WO2005047521A2 (en) 2003-11-10 2004-11-10 Methods of preparing nucleic acid for detection

Country Status (4)

Country Link
US (2) US20070042400A1 (en)
EP (1) EP1694869A2 (en)
JP (1) JP2007512811A (en)
WO (1) WO2005047521A2 (en)

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