WO2005056755A3 - Compositions and methods for propagation of neural progenitor cells - Google Patents

Compositions and methods for propagation of neural progenitor cells Download PDF

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Publication number
WO2005056755A3
WO2005056755A3 PCT/US2004/040224 US2004040224W WO2005056755A3 WO 2005056755 A3 WO2005056755 A3 WO 2005056755A3 US 2004040224 W US2004040224 W US 2004040224W WO 2005056755 A3 WO2005056755 A3 WO 2005056755A3
Authority
WO
WIPO (PCT)
Prior art keywords
neural progenitor
progenitor cells
propagation
compositions
methods
Prior art date
Application number
PCT/US2004/040224
Other languages
French (fr)
Other versions
WO2005056755A2 (en
Inventor
Oleg V Kopyov
Original Assignee
Catholic Healthcare West
Oleg V Kopyov
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Catholic Healthcare West, Oleg V Kopyov filed Critical Catholic Healthcare West
Priority to CN2004800396751A priority Critical patent/CN101065478B/en
Priority to JP2006542710A priority patent/JP4676442B2/en
Priority to CA2547827A priority patent/CA2547827C/en
Priority to EP04812676.7A priority patent/EP1709151B1/en
Priority to ES04812676.7T priority patent/ES2579804T3/en
Publication of WO2005056755A2 publication Critical patent/WO2005056755A2/en
Publication of WO2005056755A3 publication Critical patent/WO2005056755A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0623—Stem cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0221—Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0226—Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/14—Calcium; Ca chelators; Calcitonin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/148—Transforming growth factor alpha [TGF-a]

Abstract

Compositions and methods for the culturing, propagation, cryopreservation and manipulation of neural progenitor cells (NPC) and pluripotent stem cells (PSC) are provided. The cells exhibit rapid doubling times and can be maintained in vitro for extended periods. Also provided is a method of propagating neural progenitor cells, and a method of transplanting human NPC and/or PSC to a host. The cells can be genetically modified to express a therapeutic agent prior to the transplanting.
PCT/US2004/040224 2003-12-02 2004-12-02 Compositions and methods for propagation of neural progenitor cells WO2005056755A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CN2004800396751A CN101065478B (en) 2003-12-02 2004-12-02 Compositions and methods for propagation of neural progenitor cells
JP2006542710A JP4676442B2 (en) 2003-12-02 2004-12-02 Compositions and methods for growing neural progenitor cells
CA2547827A CA2547827C (en) 2003-12-02 2004-12-02 Compositions and methods for propagation of neural progenitor cells
EP04812676.7A EP1709151B1 (en) 2003-12-02 2004-12-02 Compositions and methods for propagation of neural progenitor cells
ES04812676.7T ES2579804T3 (en) 2003-12-02 2004-12-02 Compositions and methods for the propagation of neural progenitor cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US52624203P 2003-12-02 2003-12-02
US60/526,242 2003-12-02

Publications (2)

Publication Number Publication Date
WO2005056755A2 WO2005056755A2 (en) 2005-06-23
WO2005056755A3 true WO2005056755A3 (en) 2007-07-05

Family

ID=34676605

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/040224 WO2005056755A2 (en) 2003-12-02 2004-12-02 Compositions and methods for propagation of neural progenitor cells

Country Status (7)

Country Link
US (1) US7632681B2 (en)
EP (1) EP1709151B1 (en)
JP (1) JP4676442B2 (en)
CN (1) CN101065478B (en)
CA (1) CA2547827C (en)
ES (1) ES2579804T3 (en)
WO (1) WO2005056755A2 (en)

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US20070269412A1 (en) 2003-12-02 2007-11-22 Celavie Biosciences, Llc Pluripotent cells
WO2005072790A1 (en) 2004-02-02 2005-08-11 I.M.T. Interface Multigrad Technology Ltd. Device for directional cooling of biological matter
US7935478B2 (en) 2004-02-02 2011-05-03 Core Dynamics Limited Biological material and methods and solutions for preservation thereof
JP5096148B2 (en) 2004-06-07 2012-12-12 コア・ダイナミクス・リミテッド Method for disinfecting biological samples
EA200700395A1 (en) 2004-08-02 2008-12-30 Оулстоун Лтд. SPECTROMETER OF ION MOBILITY
WO2006016372A1 (en) 2004-08-12 2006-02-16 I.M.T. Interface Multigrad Technology Ltd. Method and apparatus for freezing or thawing of a biological material
US8198085B2 (en) 2005-08-03 2012-06-12 Core Dynamics Limited Somatic cells for use in cell therapy
US8114668B2 (en) * 2007-05-14 2012-02-14 Cardiac Pacemakers, Inc. Composition for cold storage of stem cells
US8283160B2 (en) 2007-09-11 2012-10-09 Frey Ii William H Methods, pharmaceutical compositions and articles of manufacture for administering therapeutic cells to the animal central nervous system
US20100209399A1 (en) * 2009-02-13 2010-08-19 Celavie Biosciences, Llc Brain-derived stem cells for repair of musculoskeletal system in vertebrate subjects
US11963977B2 (en) * 2012-04-24 2024-04-23 Vcell Therapeutics Inc. Generating pluripotent cells de novo
CA3143449A1 (en) 2013-03-14 2014-09-25 The Regents Of The University Of California In vitro production of medial ganglionic eminence precursor cells
CN104450619B (en) * 2014-12-11 2018-04-27 温州医科大学附属第一医院 The cultural method of rat pituitary prolactin pituitary cell line Multidrug-resistant
EP3945815A4 (en) * 2019-04-03 2023-04-19 Akron Biotechnology, LLC Cryopreservation and cell culture media
CN116410921B (en) * 2023-02-09 2024-01-23 北京益华生物科技有限公司 Human umbilical cord mesenchymal stem cell induction culture medium, induction method and application

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Also Published As

Publication number Publication date
CA2547827C (en) 2015-02-03
CA2547827A1 (en) 2005-06-23
JP4676442B2 (en) 2011-04-27
JP2007524405A (en) 2007-08-30
EP1709151A2 (en) 2006-10-11
CN101065478B (en) 2012-07-04
WO2005056755A2 (en) 2005-06-23
CN101065478A (en) 2007-10-31
EP1709151B1 (en) 2016-04-13
EP1709151A4 (en) 2008-01-02
ES2579804T3 (en) 2016-08-16
US7632681B2 (en) 2009-12-15
US20050118561A1 (en) 2005-06-02

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