WO2005067410A3 - Synthetic nucleic acids from aquatic species - Google Patents

Synthetic nucleic acids from aquatic species Download PDF

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Publication number
WO2005067410A3
WO2005067410A3 PCT/US2003/037117 US0337117W WO2005067410A3 WO 2005067410 A3 WO2005067410 A3 WO 2005067410A3 US 0337117 W US0337117 W US 0337117W WO 2005067410 A3 WO2005067410 A3 WO 2005067410A3
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WO
WIPO (PCT)
Prior art keywords
nucleic acid
synthetic nucleic
nucleic acids
acid molecule
acid sequence
Prior art date
Application number
PCT/US2003/037117
Other languages
French (fr)
Other versions
WO2005067410A2 (en
Inventor
Brian D Almond
Monika G Wood
Keith V Wood
Original Assignee
Promega Corp
Brian D Almond
Monika G Wood
Keith V Wood
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Promega Corp, Brian D Almond, Monika G Wood, Keith V Wood filed Critical Promega Corp
Priority to CA002525582A priority Critical patent/CA2525582A1/en
Priority to AU2003297293A priority patent/AU2003297293B2/en
Priority to JP2005513754A priority patent/JP2006512098A/en
Priority to EP03819255A priority patent/EP1654359A4/en
Publication of WO2005067410A2 publication Critical patent/WO2005067410A2/en
Publication of WO2005067410A3 publication Critical patent/WO2005067410A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
    • C12N15/8212—Colour markers, e.g. beta-glucoronidase [GUS], green fluorescent protein [GFP], carotenoid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

A synthetic nucleic acid molecule is provided that includes nucleotides of a coding region for a fluorescent polypeptide having a codon composition differing at more than 25% of the codons from a parent nucleic acid sequence encoding a fluorescent polypeptide. The synthetic nucleic acid molecule has at least 3-fold fewer transcription regulatory sequences relative to the average number of such sequences in the parent nucleic acid sequence. The polypeptide encoded by the synthetic nucleic acid molecule preferably has at least 85% sequence identity to the polypeptide encoded by the parent nucleic acid sequence.
PCT/US2003/037117 2002-12-09 2003-11-20 Synthetic nucleic acids from aquatic species WO2005067410A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA002525582A CA2525582A1 (en) 2002-12-09 2003-11-20 Synthetic nucleic acids from aquatic species
AU2003297293A AU2003297293B2 (en) 2002-12-09 2003-11-20 Synthetic nucleic acids from aquatic species
JP2005513754A JP2006512098A (en) 2002-12-09 2003-11-20 Synthetic nucleic acids of aqueous species
EP03819255A EP1654359A4 (en) 2002-12-09 2003-11-20 Synthetic nucleic acids from aquatic species

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/314,827 US20030157643A1 (en) 2000-08-24 2002-12-09 Synthetic nucleic acids from aquatic species
US10/314,827 2002-12-09

Publications (2)

Publication Number Publication Date
WO2005067410A2 WO2005067410A2 (en) 2005-07-28
WO2005067410A3 true WO2005067410A3 (en) 2005-12-08

Family

ID=34794118

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/037117 WO2005067410A2 (en) 2002-12-09 2003-11-20 Synthetic nucleic acids from aquatic species

Country Status (7)

Country Link
US (2) US20030157643A1 (en)
EP (1) EP1654359A4 (en)
JP (1) JP2006512098A (en)
KR (1) KR20050105977A (en)
AU (1) AU2003297293B2 (en)
CA (1) CA2525582A1 (en)
WO (1) WO2005067410A2 (en)

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US6602677B1 (en) * 1997-09-19 2003-08-05 Promega Corporation Thermostable luciferases and methods of production
US7879540B1 (en) * 2000-08-24 2011-02-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
US20030157643A1 (en) * 2000-08-24 2003-08-21 Almond Brian D Synthetic nucleic acids from aquatic species
US7413874B2 (en) * 2002-12-09 2008-08-19 University Of Miami Nucleic acid encoding fluorescent proteins from aquatic species
US7960530B2 (en) * 2003-06-16 2011-06-14 Riken Fluorescent protein
DE102004024817B3 (en) * 2004-05-17 2005-12-08 Miele & Cie. Kg Vacuum cleaner with a multipart device housing
JP4695073B2 (en) * 2004-05-20 2011-06-08 独立行政法人理化学研究所 Fluorescent protein
US7728118B2 (en) * 2004-09-17 2010-06-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
KR101273829B1 (en) * 2005-05-20 2013-06-11 론자 바이올로직스 피엘씨 High-level expression of recombinant antibody in a mammalian host cell
WO2007120166A2 (en) * 2005-07-11 2007-10-25 University Of Florida Research Foundation, Inc. Miniaturized in vitro protein expression array
SG190572A1 (en) 2008-04-29 2013-06-28 Abbott Lab Dual variable domain immunoglobulins and uses thereof
CA2726087A1 (en) 2008-06-03 2009-12-10 Tariq Ghayur Dual variable domain immunoglobulins and uses thereof
US9145447B2 (en) * 2009-12-04 2015-09-29 Allele Biotechnology & Pharmaceuticals, Inc. Photoconvertible fluorescent proteins
UY33492A (en) 2010-07-09 2012-01-31 Abbott Lab IMMUNOGLOBULINS WITH DUAL VARIABLE DOMAIN AND USES OF THE SAME
KR20130100118A (en) 2010-08-03 2013-09-09 아비에 인코포레이티드 Dual variable domain immunoglobulins and uses therof
US10928340B2 (en) * 2018-06-14 2021-02-23 The Boeing Company Method and apparatus for controlling contact of composite tows

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Also Published As

Publication number Publication date
KR20050105977A (en) 2005-11-08
CA2525582A1 (en) 2005-07-27
AU2003297293A1 (en) 2005-09-08
WO2005067410A2 (en) 2005-07-28
EP1654359A4 (en) 2006-11-22
AU2003297293B2 (en) 2007-09-13
US20090191622A1 (en) 2009-07-30
US20030157643A1 (en) 2003-08-21
EP1654359A2 (en) 2006-05-10
JP2006512098A (en) 2006-04-13

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